B형 간염바이러스의 태아와 성인간세포에 대한 감염성 해명 연구

정태호,김성국,서화정,윤영국,임종완,차상호 경북대학교 의학연구소 1999 경북대학교병원의학연구소논문집 Vol.3 No.1

사람의 태아와 성인의 간세포에 대한 B형 간염바이러스의 감염도를 비교 분석함으로써 HBV 와 간세포간의 친화도내지는 HBV 수용체에 관한 정보를 얻고자 함 대상 및 방법 : 성인이 외상을 입어 부득이 손상된 간조직을 절제하였을 경우와 모자보건법상 임신지속이 어려운 상태에서 부인과적 수술시 21주 전후의 태아 간조직을 얻어 DMEM 을 배지로 50% 용량으로 HBsAg 양성인 사람 혈청을 가하여 5일간 체외 배양하고 병리조직학적 처리후 효소 면역학적 방법으로 HBsAg 를 염색하여 바이러스의 존재 여부를 검색하였다. 결과 : 태아와 성인 간조직을 5일간 체외 배양하였을 때 그 조직상은 간조직임을 인지할 수 있었다. HBV 의 감염여부는 DAKO 사의 효소면역 법으로 조사한 결과 태아의 간조직에서는 HBsAg 양성부위를 발견하지 못하였으나, 성인 간조직에서는 HBsAg 양성부위가 발견되었다. 결론 : 태생 21주 전후의 태아의 간세포는 HBV 와 친화도는 아주 미약하거나 없고, 성인 간세포는 HBV에 대한 친화도가 높다. 따라서 태생 21주의 간세포에는 HBV 의 감염이 어렵다는 것을 인지하게 되었다. Considerable progress has been made in the immune prophylaxis of hepatitis B virus(HBV) infection and in the understanding of its replication and pathogenecity at the molecular level. However, a convenient in vitro assay for HBV infectivity has been lacking and the early steps of viral life cycle are now yet well understood. Primary cultured human hepatocytes are reported to be susceptible to HBV 10-12, but these systems are not practical because they are short-lived and require primary explanted human liver. However, it is uncertain yet whether primary hepatocytes of human fetal liver are susceptible to HBV for infection or not. We have expanded the infectivity of HBV into a primary cultures of human fetal hepatocytes together with controversy of adult primary hepatocytes. To investigate the infectivity of HBV for, adult and fetal hepatocytes After 5 day culturing fetal(1) and adult(2) liver tissues, which obtained from a surgical operation on DMEM culture media with half HBV positive serum, separating liver cells and fixing them, the existence of HBsAg antigen was investigated through the immuno-enzymatic staining. HBsAg stained area was not found in the 21 weeks gestation liver cells until 5 days of invitro-culture, while it was found in the adult liver cells. This test result showed that the HBV infection is very hard to happen in the liver cells 21 weeks gestation or prior one.

光州直轄市 地域의 經濟基盤에 관한 硏究

鄭英煥,金鍾華 德成女子大學校 1989 德成女大論文集 Vol.18 No.-

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환경행정공무원의 전문성과 환경행정의 규제성에 관한 조사연구 : 2001, 2003년 조사사례를 중심으로

정용택,박종안,손부순,이종화,장봉기,박정임 순천향대학교 기초과학연구소 2008 순천향자연과학연구 논문집 Vol.14 No.1

We have analyzed this influence base on survey of central government administration officers and local officers which were taken on 2001 and 2003. Also, we anticipates that this result would help actual field adjustment as well as preparation of speciality of officers. The results are summarized as follows : First, a survey in 2001 shows 68.8 percent of local administration officers answered that their expertise about environmental services were above average. Then, the result was increased to 82 percent of officers responsed that they have more than average expertise and experience in 2003 survey. Secondly, government have to increase effectiveness on regulation or restriction duty. Nonetheless, the survey shows that significant decrease of effectiveness from 2001 to 2003 in both central and local goveniment officers. It means that they spent more effort on local community environmental regulation program other than restriction duty to requisited from central government. Third, moreover, local government do not want to faculty of environmental administration and insists that governments need to set the regulation for particularity of regions.

역량스펙트럼 방법을 이용한 철근 콘크리트 교각의 내진성능 평가

송종걸,장동휘,정영화 江原大學校 産業技術硏究所 2004 産業技術硏究 Vol.24 No.A

To evaluate seismic performance of reinforced concrete piers two procedures for capacity spectrum method are presented. The capacity spectrum procedures include the reduction factor-ductility-period(R_(μ)-μ-T)relationship in order to construct the inelastic demand spectra from the elastic demand spectra. Application of the procedures are illustrated by example analysis. Maximum displacements estimated by the procedures are compared to those by inelastic time history analysis for several artificial earthquakes. The results show that the maximum displacements estimated by the procedures are, on overall, smaller than those by the inelastic time history analysis.

大豆未熟子葉祖織의 營養培地條件에 따른 器官分化

元鍾樂,權貞淑,鄭圭和,張權烈 慶尙大學校 1987 論文集 Vol.26 No.1

大豆의 末熟子葉組織을 사용하여 여러가니 營養培地에서 칼루스와 胚狀體로부터 器官을 分化시키고자 하였던 바 얻어진 결과를 要約하면 다음과 같다. 1. 칼루스誘起는 MS 基本培地에 1.0mg/12,4-D,와 2.0mg/1kinetin을 첨가하거나 (M5培地) 하였을 때 가장 효과적이었다. 2. M4培地에 培養한 칼루스로부터 生長點모양의 완전한 구조를 갖춘 細胞群을 組織學的으로 관찰할 수 있었다. 3.칼루스를 器官分化를 위한 培地에 ?代培養하였을때 根分化, 칼루스로부터胚狀體의 形成, 胚狀體로부터 싹이나 잎의 分化는 供試한 大豆系統이나 使用한 營養培地에 따라 약간의 差異는 있으나 비교적 용이할 것으로 여겨졌다. 4. 供試한大豆系統인 뱀콩의 末熟子葉組織을 M4培地에서 칼루스를 誘起시킨 후 MS基本培地에 0.005mg/1 picloram 과 2.0 mg/1 BAP를 첨가한 培地(OM2培地)에 ?代培養하였을 때 칼루스로부터 胚狀體의 形成이 잘 되었고 胚狀體들로 부터 잎이 分化되었다. The experiments were conducted to investigate organ differentatin rom calls and embryoid under various media using immature ctyledon of soybeam. The results obtained were as followings: 1. 1. Omg 2,4-D plus 0.2mg kinetin or 0.2 mg BAP perr litre on MS basal mediu were efective growth regulators for callus induction. 2. Meristematic structure was observed from the callus induced by 1.0mg 2,4-D plus 0.2mg kinetin per litre in MS basal medium in which callus growth showed best response. 3. Root and embryoid from callus and shoot or leaflet from embryoid were differentiated on the medium subcultured for organ different on the soybean lines and media applied. 4. Embryoid from callus and leaflet from the embryoid were differentiated on the nutritional condition subscultured with 0.005mg picloram plus 2.0mg kinetin per litre on MS in immature cotyledon of spybean line Baemkong.

세라믹 브라켓의 제거 시 Er : YAG 레이저의 효능

서충환,장나영,채종문,조진형,김상철,강경화 대한치과교정학회 2009 대한치과교정학회지 Vol.39 No.4

본 연구에서는 세라믹 브라켓 제거에 Er:YAG 레이저 조사가 도움이 되는지 알아보고, 브라켓 제거에 적합한 레이저 조사 방법을 연구하였으며, 또 이렇게 적용된 레이저가 치수와 법랑질에 손상을 주는지도 알아보았다. 총 190개의 치아, 단결정 세라믹 브라켓(MISO), 다결정 세라믹 브라켓(Transcend series 6000)과 KEY Laser3를 사용하였다. 실험군은 세라믹 브라켓의 종류(단결정, 다결정)와 레이저의 에너지(140, 300, 450, 600 mJ)에 따라 분류하였으며, 레이저를 브라켓당 두 곳에 1펄스씩 조사하고, 전단 강도를 측정하였다. 대조군은 레이저를 조사하지 않는 군으로 하였다. 레이저 조사에 의한 열 효과는 브라켓 하방 법랑질과 치수강에서 측정하였으며, 전단 강도 측정 후 치면에 남아있는 접착제의 양을 접착제 잔류 지수(adhesive remnant index)를 이용하여 평가하였다. 레이저 조사로 인한 접착제의 파괴 양상과 법랑질 표면 변화를 주사전자현미경으로 관찰하였다. 모든 세라믹 브라켓군에서 레이저 에너지가 증가 할수록 전단 강도는 유의하게 감소하였다. 또한, 브라켓 하방 법랑질에서 최대 온도 변화는 평균 3.78˚C 상승에 그쳤으며, 치수강에서 최대 온도 변화는 평균 0.9˚C 상승에 그쳤다. 주사전자현미경을 이용한 법랑질과 접착제 단면 관찰에서 접착제 표면이 레이저에 의해 붕괴되어 분화구 모양의 구덩이로 관찰되었으며, 일부 시편에서 약 10 - 30μm의 법랑질 손상이 발견되었다. Transbond XT로 부착된 단결정 도재 브라켓(MISO)의 디본딩에 Er:YAG 레이저를 이용할 경우, 300 - 450 mJ의 레이저 에너지를, 그리고 다결정 도재 브라켓(Transcend series 6000)의 경우는 450 mJ 정도의 에너지를 사용하는 것이 효과적이고 안전할 것으로 생각된다. Objective: The aim of this study was to find out whether Er:YAG laser can aid in debonding ceramic brackets, and to see what kind of method will be the most appropriate for debonding. Methods: One hundred and ninety teeth, monocrystalline brackets (MISO, HT, Ansan-Si, Korea), polycrystalline brackets (TranscendTM series 6000, 3M Untek, Monrovia, CA, USA) and the KEY Laser3 (KavoDental, Biberach, Germany) were used. Experimental groups were classified according to the type of ceramic brackets, and the amount of laser energy (0, 140, 300, 450, 600 mJ). After applying laser on the bracket at two points at 1 pulse each, the shear bond strength was measured. The effect of heat caused by laser was measured at the enamel beneath the bracket and pulp chamber. After measuring the shear bond strength, adhesive residue was evaluated and enamel surface was investigated using SEM. Results: All ceramic bracket groups showed a significant decrease in shear bond strength as the laser energy increased. The greatest average temperature change was 3.78˚C on the enamel beneath the bracket and 0.9˚C on the pulp chamber. Through SEM, crater shape holes caused by the laser was seen on the enamel and adhesive surfaces. Conclusions: If laser is applied on ceramic brackets for debonding, 300 - 450 mJ of laser energy will be safe and efficient for monocrystalline brackets (MISO), and about 450 mJ for polycrystalline brackets (Transcend series 6000).

웨이블렛 영역에서 Interleaving을 이용한 Gray-level Image Watermarking

이종원,고성식,김정화 조선대학교 전자정보통신연구소 2001 電子情報通信硏究所論文誌 Vol.4 No.2

Watermarking is an emerging technology that is claimed to have an important application in copyright protection. Compared with existing watermarking method, the size of watermark image is not only smaller than that of original image, but data quantity of watermark image is restricted. Thus in this paper, we propose a new watermark method which can embed a 256 Gray-Scale watermark invisibly in wavelet transform domain and enhance image quality by interleaving to keep robustness. Also, it used Median Filter with after control, it improved an image quality. Using the interleaving proposed in this paper, experimental results demonstrate that high quality recovery of the watermark is possible to operations such as LPF or JPEG lossy compression.

횡하중 분배방법을 이용한 교량구조물의 비탄성 변위응답 평가

송종걸,남왕현,정영화 江原大學校 産業技術硏究所 2005 産業技術硏究 Vol.25 No.A

In order to evaluate seismic performance of multi-degree-of-freedom bridge structure, moderate lateral load distribution methods using the pushover analysis were developed by many researchers. One of important variables to improve an accuracy of pushover analysis is lateral load distribution. In this study, pushover analyses were performed using the five types of lateral load distribution and seismic performances were evaluated by capacity spectrum method (CSM). To verify an accuracy of suggested lateral load distribution, the maximum displacement estimates by the CSM were compared to those by inelastic time history analysis.

등가단자유도 방법에 따른 다자유도 교량의 지진응답평가

송종걸,남왕현,정영화 江原大學校 産業技術硏究所 2005 産業技術硏究 Vol.25 No.A

The capacity spectrum method(CSM) can be used for the evaluation of inelastic maximum response of structures and has been recently used in the seismic design using the incorporation of pushover analysis and response spectrum method. To efficiently evaluate seismic performance of multi-degree-of freedom(MDOF) bridge structures, it is important that the equivalent response of MDOF bridge structures should be calculated. To calculate the equivalent response of MDOF system, equivalent responses are obtained by using Song method, Fajfar method and Calvi method. Also, those responses are applied to CSM method and seismic performance of bridge according to the ESDOF method are compared and evaluated straightforwardly.

감의 脫澁處理中 Pectin 含量과 Texture의 變化

崔鍾旭,孫泰華,鄭信敎,李吉雨,姜峻洙 慶北大學校 1989 論文集 Vol.47 No.-

This experiment was made to investigate the changes of the contents of acetaldehyde, alcohol, tannin, and to study the texture changes of persimmon in relation to the contents of pection during the removal of astringency by CO_2. 1. During the removal of astringency of persimmon fruits, the contents of soluble tannin showed the rapid decrease in high concentration of CO_2, but the contents of ethanol and acetaldehyde showed the rapid increase in all treatments. 2. The contents of W.S.P. increased, but H.S.P. decreased in all treatments. 3. All texture paramenters of persimmon fruits except springness and adhesiveness were decreased druing the removal of astringency. 4. The contents of W.S.P. were good correlated with the changes of hardness in all treatments.