두경부편평상피암 연구의 3차원적 In Vitro 실험 모델 확립 : Spheroid 모델과 Raft 배양모델 Spheroid Model and Raft Culture Model

변성완,김춘동 梨花女子大學校 醫科大學 醫科學硏究所 2001 EMJ (Ewha medical journal) Vol.24 No.1

Objectives : To establish new in vitro model systems that better reflect in vivo condition, multicellular tumor spheroids(MTS) and raft culture were developed using cell lines of squamous cell carcinoma(SCCHN) of the head and neck in these 3-dimensional systems. Materials and Methods : Four SCCHN cell lines were used for MTS and raft culture. Results : All cell lines formed MTS, but only Tu-138 showed a good stratification at the airliquid interface in the raft culture system. Conclusion : MTS and raft culture system were established successfully from the SCCHN cell lines.

소아에서 구개 및 인두편도 절제술 후 체중 변화

김춘동,변성완 梨花女子大學校 醫科大學 醫科學硏究所 2001 EMJ (Ewha medical journal) Vol.24 No.1

Objectives : There are controversial evidences that children gain weight to a significant extent after adenotonsillectomy, usually associated with a general improvement in health. This study is aimed to evaluate the growth disturbance in children with adenotonsillar hypertrophy and the effect of adenotonsillectomy on subsequent growth in them. Methods : Ninty-one children adenotonsillectomized had their weights recorded preoperatively, and reweighted twelve months after surgery. Results : The results showed the patients were generally not underweighted before surgery. Their median weight was on the 59.5 percentile. Their weights after surgery had increased by an average of 20 percent over what would have been expected. This increase in weight was not confined to underweight children but to the entire group. Conclusion : Upper airway obstruction resulting from adenotonsillar hypertrophy might be suspected as a possible cause in the workup of children with suboptimum growth.

발달장애유아의 언어행동발달 연구

김춘희,변찬석,서경희,유은정,이효신,전헌선,최성규 大邱大學校 特殊敎育·再活科學硏究所 2000 特殊敎育硏究 Vol.23 No.1

본 논문은 발달장애유아 언어행동발달 컨텐츠 개발에 요구되는 기초연구로써 연구의 이론적 바탕을 확립하고자 하였다. 연구에 사용된 방법은 문헌조사 방법 및 현장 인터뷰기법을 병행하였다. 본 연구의 주된 목적은 크게 3가지로 구분된다. 첫째, 발달장애유아의 가정 내 교육 욕구를 분석하고자 하였다. 둘째, 발달장애유아의 언어발달 양상을 검토하고자 하였다. 세 번째 목적은 유아의 언어행동발달 컨텐츠 구성의 주요 지침을 설정하고자 하였다. 이상의 연구목적에 따른 연구결과를 요약하면 다음 몇 가지로 제시된다. 1.가정 내 부모-유아간 관계형성이 우선 고려 되어야하고, 이에 따른 상호작용은 발달적 애착형성이 가능한 방향으로 정립되어야한다. 2.발달장애유아의 언어행동발달양상을 검토한 결과에 따르면, Piaget는 언어행동발달에 있어 감각운동기적 환경탐색 기능을 강조하고 있고, 이는 현재까지 중요한 유아 발달의 기저로 정착되었다. 따라서, 발달장애유아의 언어행동발달을 위한 컨텐츠 개발 내용에 있어서도 이러한 기저에 충실함이 합리적이다. 3.발달장애유아들에게는 언어행동 양상의 갈래가 크게 운동장애 및 감각결함으로 나누어질 수 있으므로, 이 두 가지 갈래의 각기 다른 특성이 컨텐츠 구성에서 충분히 고려되어야한다. 4.자폐성과 같은 중증 전반적 발달장애 유아를 위한 언어행동발달 컨텐츠 구성에서 특히 고려해야 할 두 가지 요소는 모방행동기능과 반복단순훈련기능 향상으로 결론지을 수 있다. This article planed to establish the theoretical foundations of the research as a basic study needed for developing the contents of verbal(language) behavior development for the infants with developmental disabilities. The methods used in the study was previous literature researches and interviews with subjects. The purposes of the study were divided into three parts. First, it was to analyze the educational needs of the infants with developmental disabilities in home settings. Second, it was to review and discover the language patterns of the infants with developmental disabilities. The third purpose was to devise the major guidelines of their language development's contents. The results of the study were described as the followings. 1.The relationships between the infants with developmental disabilities and their parents and their interactions should move to build the developmental attachments between them. 2.According to the literature findings of their language development patterns, Piaget concentrated on the functions of sensory motor environmental exploring in the language behavior development. The functions have been settled down into the important foundation for the infants' development. Thus, it was resonable that the content's development of the language behaviors for the infants with developmental disabilities should based on the function addressed by Piaget. 3.The language behaviors' patterns of the infants with developmental disabilities generally were classified into motor disabilities and sensory defect(deficiency). Therefore, the two factors should be considered for the constructions of the contents. 4.Constructing the contents of the language behavior developments for the infants with severely pervasive developmental disabilities such as autism, the improvements of two functions, imitative behavior and repetitive simple training should be considered.

아로마 요법이 신생아의 스트레스 호르몬에 미치는 영향

김재철,천성희,변상현 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

Aromatherapy, one of complementary medicine was known that specific essential oil extracted from plants affect emotion and physiology of human by transmission of smell signal from olfactory cells to limbic system of brain. This study was intended to reveal the effect of aromatherapy by comparison of stress hormone and vital sign between the aromatherapy group and the control group. From June, 2001 to August, 2002, the aromatherapy group(n=12) and the control group (n=13) were selected randomly in NICU, Chungnam National University Hospital. We applied aromatherapy to the aromatherapy group 3 times every 20 minutes for 7 days and 24 hours urine collection for free cortisol and epinephrine was performed day 0(before initial aromatherapy) and day 7(after the last aromatherapy). After aromatherapy, no difference of change of vital sign was noted in aromatherapy group. Epinephrine concentration after aromatherapy decreased from 1.28±1.13μg/d to 0.84±0.58μg/d(p-value<0.05). Free cortisol concentration after aromatherapy decreased from 4.74±3.99μg/d to 1.60±0.84μg/d.(p-value<0.05). At birth, there was a marked increase in plasma stress hormone and after birth, stress hormone of neonate except acute or chronic ill baby decrease gradually. But in aromatherpy group, neonatal stress hormone decreased faster than nonaromatherapy group.

측정방법에 따른 치과용 수경성 시멘트의 경화시간

박정우,기형춘,변상기,이해형 大韓齒科器材學會 2001 대한치과재료학회지 Vol.28 No.4

Information on setting behavior of luting cements is essential to accomplish successful cementation of dental restoration. The purpose of this study is to investigate the influence of test variables on the setting time of dental water-based luting cements. Six kinds of luting cements were investigated: two zinc phosphate, two zinc polycarboxylate and two glass ionomer cements. Cements were mixed with the standard consistency for luting according to their respective manufacturer's recommendation. Setting time of cements was measured with two test apparatus (penetrating indentor and oscillating rheometer) under one of following environmental conditions (temperature/humidity); 37℃/90%, 23℃/50% and 37℃/20%. The Setting time was recorded as the time elapsed from start of mixing to the time indicated at the ISO specification (9917:1991) or when the oscillation is reduced to 95% of the original width. Setting time of luting cements was decreased with increase in temperature and decrease in humidity, and it was significantly influenced by environmental temperature and humidity. Some cements tested showed significant differences in setting time measured between indentor and rheometer under laboratory environment. Percent water loss of cements was different under various environmental conditions. This was largely arisen at the early stage of setting. Zinc Phosphate was hygroscopic under high humidity. these results indicate that working and setting time should be separately evaluated in office and oral environments, respectively. Oscillating rheometer was useful to investigate setting behavior of dental water-based luting cement. However, control of test environment and sensitivity should be considered in the rheometry.

국내에서 유행하는 HIV의 전파 경로에 따른 Subtype 분포

이주실,남정구,김성순,강춘,최병선,김옥진,박미선,성봉모,서순덕,전수경,변승옥,신영오,조해월 대한감염학회 2001 감염 Vol.33 No.5

Background : Previous data have been reported that subtype B is prevalent in South Korea, but neither the extent nor the proportion of subtypes could be evaluated. This study was designed to analyze the distribution of HIV-1 subtypes, temporal instructions and transmission dynamics between epidemiological groups. Methods : 1,280 Koreans had been diagnosed as HIV seropositive during the period 1985 to 2000. Among them, 134 individuals were selected for this molecular epidemiological study. 134 DNAs were isolated from uncultured or cultured peripheral blood mononuclear cells. V3-V5 (0.7 kb) fragment of HIV-1 env gene was amplified by nested polymerase chain reaction and was sequenced. Results : HIV-1 isolates from thirty-seven homosexuals were all subtype B (100%). On the other hand, 66 isolates from 94 heterosexuals were subtype B (70%) and 28 were non B subtypes (30% : 13 A, 4 C, 2 D, 8 E , 1 G). Only subtype B strains were isolated from 73 males who were infected with HIV inside Korea while 16 B and 20 non B subtype strains were isolated from 36 males who were HIV infected outside of Korea. However, B and non B strains were isolated half and half from females who were infected inside Korea except one. Conclusion : The HIV-1 subtype B strains are prevalent in Korea from the early HIV infection until present in both homo and heterosexuals. Non B strains have been transmitted from men who were infected outside Korea to their spouses and casual partners. So, we need further study to monitor subtype B and non B HIV transmission in epidemiological groups of Korea, (Korean J Infect Dis 33:311∼318, 2001)

Bark Constituents from Mushroom-detoxified $Rhus$ $verniciflua$ Suppress Kainic Acid-induced Neuronal Cell Death in Mouse Hippocampus

Byun, Jong-Seon,Han, Yoon-Hee,Hong, Sung-Jun,Hwang, Sung-Mi,Kwon, Yong-Soo,Lee, Hee-Jae,Kim, Sung-Soo,Kim, Myong-Jo,Chun, Wan-Joo The Korean Society of Pharmacology 2010 The Korean Journal of Physiology & Pharmacology Vol.14 No.5

Urushinol, a plant allergen, has significantly restricted the medical application of $Rhus$ $verniciflua$, although it has been reported to possess a wide variety of biological activities such as anti-inflammatory, antioxidant, and anti-cancer actions. To reduce the urushinol content while maintaining the beneficial biological activities, mushroom-mediated fermentation of $Rhus$ $verniciflua$ was carried out and this method resulted in significantly attenuated allergenicity [1]. In the present study, to examine the neuroprotective properties of mushroom-fermented stem bark of $Rhus$ $verniciflua$, two constituents were isolated from mushroom-fermented bark and their neuroprotective properties were examined in a mouse model of kainic acid (KA)-induced excitotoxicity. KA resulted in significant apoptotic neuronal cell death in the CA3 region of mouse hippocampus. However, seven daily administrations of RVH-1 or RVH-2 prior to KA injection significantly attenuated KA-induced pyramidal neuronal cell death in the CA3 region. Furthermore, pretreatment with RVH-1 and RVH-2 also suppressed KA-induced microglial activation in the mouse hippocampus. The present study demonstrates that RVH-1 and RVH-2 isolated from $Rhus$ $verniciflua$ and detoxified using mushroom species possess neuroprotective properties against KA-induced excitotoxicity. This leads to the possibility that detoxified $Rhus$ $verniciflua$ can be a valuable asset in herbal medicine.

신경회로망을 이용한 전기자동차용 바테리 잔존용량계

변성천(Sung-Chun Byun),김의선(Eui-Sun Kim),류영재(Young-Jae Ryoo),임영철(Young-Cheol Lim) 대한전기학회 1998 대한전기학회 학술대회 논문집 Vol.1998 No.7

두경부암 환자의 등록 체제

변성완(Sung Wan Byun),김춘동(Chun Dong Kim),홍순관(Soon Kwan Hong),성명훈(Myung Whun Sung),김광현(Kwang Hyun Kim) 대한두경부종양학회 1998 대한두경부 종양학회지 Vol.14 No.1

재발, 추시 중 소실, 다른 원발성 암의 병발 등으로 복잡해질 수 있는 두경부암 환자 등록에 있어서, '단일등록양식다중항목체제'라는 새로운 체제로, 서울대학교 병원에서 진단한 486명의 두경부암 환자를 대상으로 하여, 데이터베이스를 구성하고, 이의 운용을 '다중등록양식체제'라는 기존의 체제와 비교하였다. 새로운 방법의 구조와 흐름이 보다 간단하고 자료의 검색이 더 효율적이었다. 두경부암 환자 등록의 전용 프로그램 개발에도 이 체제의 도입이 필요할 것으로 사료된다. Objectives: Many complicated problems exist in establishing head and neck cancer(HNCa) patient registry system. In this study, a newly devised and simplified approach named as 'Single Sheet Multiple Event System' was compared with a conventional approach named as 'Multiple Sheet System'. Material and Method: According to several kinds of registry sheets in the two systems, data were collected from 486 patients with HNCa diagnosed at Department of Otolaryngology, Seoul National University Hospital from 1979 through 1994. Results: The new system produced more simple and efficient data retrieval. Conclusion: It could make the implementation of HNCa patient registry system more simple and efficient.

늑골 골절의 위치가 동요흉의 이환율 및 사망률에 미치는 요인

변천성 ( Chun Sung Byun ),박일환 ( Il Hwan Park ),배금석 ( Geum Suk Bae ),정필영 ( Pil Yeong Jeong ),오중환 ( Joong Hwan Oh ) 대한외상학회 2013 大韓外傷學會誌 Vol.26 No.3

Purpose: A flail chest is one of most challenging problems for trauma surgeons. It is usually accompanied by significant underlying pulmonary parenchymal injuries and mayled to a life-threatening thoracic injury. In this study, we evaluated the treatment result for a flail chest to determine the effect of trauma localization on morbidity and mortality. Methods: Between 2004 and 2011, 46 patients(29 males/17 females) were treated for a flail chest. The patients were divided into two group based on the location of the trauma in the chest wall; Group I contained patients with an anterior flail chest due to a bilateral costochondral separation (n=27) and Group II contained patients with a single-side posterolateral flail chest due to a segmental rib fracture (n=19). The location of the trauma in the chest wall, other injuries, mechanical ventilation support, prognosis and ISS (injury severity score) were retrospectively examined in the two groups. Results: Mechanical ventilation support was given in 38 patients(82.6%), and 7 of these 38 patients required a subsequent tracheostomy. The mean ISS for all 46 patients was 19.08±10.57. Between the two groups, there was a significant difference in mean ventilator time (p<0.048), but no significant difference in either traumarelated morbidity (p=0.369) or mortality (p=0.189). Conclusion: An anterior flail chest frequently affects the two underlying lung parenchyma and can cause a bilateral lung contusion, a hemopneumothorax and lung hemorrhage. Thus, it needs longer ventilator care than a lateral flail chest does and is more frequently associated with pulmonary complications with poor outcome than a lateral flail chest is. In a severe trauma patient with a flail chest, especially an anterior flail chest, we must pay more attention to the pulmonary care strategy and the bronchial toilet.