Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

정준상관 행렬도와 군집분석을 응용한 KLPGA 선수의 기술과 경기성적요인에 대한 연관성 분석

최태훈,최용석,Choi, Tae-Hoon,Choi, Yong-Seok 한국통계학회 2008 응용통계연구 Vol.21 No.3

정준상관 행렬도(canonical correlation biplot)는 정준상관분석에서 두 변수 집단에 의해서 측정된 다변량 자료에서 변수 집단 간의 관계와 개체들의 관계를 탐색하기 위한 2차원 그림이다. 이는 일반적으로 최용석 (2006, 1장)의 한 변수 집단에 의한 행렬자료에 대한 일반적인 행렬도를 두 변수 집단에 의한 행렬자료로 확장한 것으로 볼 수 있다. 최근에 Choi와 Kim (2008)은 개체들이 많은 대용량 자료에서 행렬도의 해석상 힘든 문제점을 지적하고 이를 극복하는 데 군집분석을 활용하는 방법을 제시하고 있다. 일반적인 행렬도에서 발생하는 대용량 자료에 대한문제는 정준상관 행렬도에서도 동일하게 발생하곤 한다. 본 연구에서는 2006년도 KLPGA 선수 중 상금 순위 상위 50명을 대상으로 정준상관 행렬도를 통해 기술요인변수군(평균 퍼팅수. 그린 적중율, 파 세이브율, 파 브레이크율)과 경기성적요인변수군(상금, 평균 타수)간의 관련성을 살펴보고 군집분석을 활용하여 각 선수들의 군집을 시도하려한다. Canonical correlation biplot is 2-dimensional plot for investigating the relationship between two sets of variables and the relationship between observations and variables in canonical correlation analysis graphically. In general, biplot is useful for giving a graphical description of the data. However, this general biplot and also canonical correlation biplot do not give some concise interpretations between variables and observations when the number of observations are large. Recently, for overcoming this problem, Choi and Kim (2008) suggested a method to interpret the biplot analysis by applying the K-means clustering analysis. Therefore, in this study, we will apply their method for investigating the relationship between skill and competition score factors of KLPGA players using canonical correlation biplot and cluster analysis.

방울토마토중 Dichlofluanid 및 Iprodione의 생산단계별 잔류농약 경시변화

최규일,성기용,정태균,이주환,허장현,고광용,이규승 한국환경농학회 2002 한국환경농학회지 Vol.21 No.4

방울토마토의 생산단계에서의 잔류허용기준 설정과 수확후 저장기간중 경시변화 및 세척에 의한 잔류농약의 감소양상을 파악하였다. 해당약제를 안전사용기준의 기준량 및 배량을 각각 시설재배 포장에서 살포후 10일간 잔류양상을 조사하였고, 실온 및 냉장조건 하에서의 저장실험 및 세척에 따른 전류농약의 잔소량을 파악하여 생산단계부터, 출하, 저장, 소비단계까지의 잔류량을 예측할 수 있는 모델개발을 위한 자료로 이용하고자 연구를 수행하였다. Dichlofluanid와 iprodione은 포장조건에서 반감일수가 기준량 2.2, 3.3일 이었으며, 배량은 3.5, 5.4일로 나타났다. 저장조건하에서의 약제별 반감기는 포장조건보다 잔류량의 감소속도가 느린 것으로 나타나 반감일수가 증가하였고, 세척방법에 의한 잔류농약의 감소량을 조사한 결과 세제 사용시의 평균제거율은 dichlofluanid는 73.7%, iprodione은 64.3%로 나타났고, tap-water 사용시에는 dichlofluanid 73.5%, iprodione 63.5%로 나타났다. 이러한 결과는 작물의 생산단계에서 구한 합리적인 희귀식을 이용하여 잔류농약의 경시변화를 파악하고, 저장 및 세척과정을 거쳐 최종 소비단계에서의 잔류농약의 수준을 평가할 수 있었다. We studied the residual patterns of two fungicides, dichlofluanid and iprodione, in cherry tomato greenhouse after applying with the recommended and double dose. Also, the degradation patterns during storage periods of up to ten days were compared between at room temperature (20℃) and at cold temperature (4℃). Removal rates of fungicides by washing with tap-water and detergent solution (0.1%, 0.2%) were measured. Half-lives of dichlofluanid and iprodione in greenhouse cherrytomato were 2.2~3.5 and 3.3~5.4 days, respectively. During the storage period, the residues were dissipated more slow. Removal rates were 62.8~80.3% by tap-water, 60.4~83.1% by 0.1% detergent solution, and 65.3~77.6% by 0.2% detergent solution. So, we can predict of terminal residues from cultivation period to marketing, storage and consuming.

만성 심부전 환자에서의 Denopamine 투여효과

최석구,김현대,정태기,김대현,김용복,유원상 인제대학교 1994 仁濟醫學 Vol.15 No.2

만성 심부전 환자에게 새로운 경구용 비 digitalis 강심제인 denopamine 1T(5mg)을 1일 3회 3개월간 투여하여 그 유효성 및 안전성을 검토했다. Background : Patients with congestive heart failure have a high mortality rate and are also hospitalized frequently. We studied the effects of a new oral inotrophic agent, denopamine in patients with chronic heart failure. Methods : 16 patients receiving conventional treatments for heart failure were treat ed with denopamine 5mg tad for at shortest 3 months. Their history were taken and the physical examination, general laboratory test and the echocardiographic examination were done before and after treatment. Results: There were 3 deaths and 1 withdrawal because of worsening heart failure. 5 patients (42%) among 12 patients showed the improvement in their subjective symptoms. The fractional shortening and ejection fraction of the left ventricle were increased by 3.2% and 5.6% respectively in their absolute values. Conclusion : The addition of denopamine to conventional therapy considerably improved the subjective symptoms and the left ventricular function in the limited number of patients with chronic heart failure.

표고 폐골목으로 제조한 목질보드의 성질

최용순,황원중,한태형,김남훈,권진헌 江原大學校 森林科學硏究所 1998 Journal of Forest Science Vol.14 No.-

要約표고폐골목의 재활용을 목적으로 폐골목과 정상재의 칩을 일정비율로 혼합하여 얻은 목질보드의 성질을 조사, 검토하였다. 그 결과 폐골목의 목섬유는 정상재보다 다소 짧고 표 면이 거칠었으며 폐골목 변재부의 밀도는 정상재에 비해 상당히 낮았다. 파티클보드의 두께 팽윤율 및 파괴계수의 시험결과 폐골목을 25~50% 정도 혼합한 경우는 정상재와 비슷한 값 을 나타냈다.결론적으로 폐골목은 파티클보드의 재료로 사용될 수 있고 정상재와의 혼합비율은 25~50% 범위에서 조정되어야 할 것으로 생각되었다.ABSTRACTTo provide further information for reutilization of the bed-logs decayed by oak mushroomfLentinus edodes), the properties of wood-based board prepared with mixed materials of normal wood and bed-logs were examined.Wood fibers in bed-logs showed different morphology from normal wood fibers, that is, shorter length and rough surface. Thickness swelling increased with increasing mixing ratio of bed-log. On the other hand, modulus of rupture(MOR) in bending strength decreased. In products, the visual surface of board prepared from higher mixing rate of bed-log chips was smooth.Consquently, it is considered that the optimum mixing rate of bed-log to normal wood is the range of 25-50% and the bed-log can be used for raw material of board instead of normal wood.

신생아의 두뇌 초음파검사상 뇌실주위 고반향음영에 관한 연구

최영칠,이태용,조영채,이동배 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Neurosonography is an excellent modality for imaging a wide array of intracranial pathology of the infant and the newborn. Periventricular high echos were prominent for the premature infant, but there are some difficulties in differentiate normal periventricular high echo from periventricular leukomalacia. White matter indices on neurosonography of 152 healthy newborn babies were measured and analized, with their gestational age, method of delivery, birth weight, APGAR score(1 min and 5 min), diagnosis and labaratory data. The gestational age of babies at birth (p=0.0001) and the delivery with Caesarian section (p=0.0263) were related statistcally to high white matter index. The babies with metabolic disease had higher white matter index than other disease group or normal control group. On the basis of these results, We speculate that in addition to gestational age of infants, delivery method and their disease status may play an additional role in presenting ldgh periventricular echo on neurosonography. It is possible to predict the size of non pathologic periventricular high echo and to give more information for further study plan.

CRP* 의존성 maltose 대사 촉진 유전자 sfs4의 클로닝 및 염기배열 결정

최용락,정희태,조무제,정수열 東亞大學校附設遺傳工學硏究所 1996 遺傳工學硏究 Vol.- No.3

CRP (cAMP receptor protein)은 cAMP와 결합하여 cAMP-CRP 복합체를 형성하여 전사조절의 조절인자로서 작용한다. crp 유전자에 변이를 도입하여 cAMP의 비존재 상태에서 cAMP-CRP와 비슷한 기능을 가진 crp* 유전자가 도입된 대장균 MK2001 (crp*¹, cya::km)을 숙주로 사용하여 cAMP 혹은 cGMP의 비존재하에서도 mal 유전자의 발현을 촉진시키는 유전자 sfs (sugar fermentation stimulation) 수 종을 클로닝하였다. 본 실험에서는 이미 밝혀진 nlp (Ner like protein) 유전자와 같이, sfs의 새로운 유전자를 탐색하여, 그 중 sfs4의 2126bp 전 염기배열을 결정하고, 잠정적인 sfs4의 promoter 영역에는 CRP 단백질과의 결합 DNA 공통 염기배열(5' AAT TGTGA ACACCA TCACC CGT 3')이 존재함을 확인했다. lacZ 융합 유전자를 작성하여 TP2010R1과 MK2001의 균주에서 cAMP를 첨가할 경우 각각 2.3배, 1.8배의 β-galactosidase 활성이 증가 하는 것으로 보아 sfs4는 cAMP-CRP에 의해 발현 조절을 받는 것으로 나타났다 In Escherichia coli, CRP forms a complex with cAMP and acts as a transcriptional regulator of many genes, including sugar metabolism operons. The E. coli MK2001, which is introduced the altered crp*¹, is functional in the expression of lac, ara and man, in the absence of cAMP. However, the expression of mal gene is fully activated by the addition of cAMP or cGMP. The object of the study is cloning of the sfs (sugar fermentation stimulation) genes, which was involved in regulation of mal gene expression with the altered crp*¹gene, and structural analysis and characterization of the genes at the molecular level. We have cloned 5 different E. coli genes which stimulate the maltose metabolism in a crp*¹, cya::km (MK2001) background. Newly identified genes were designated as sfs. One of the sfs genes (pPC1), located at the 53.2 min map position in the E. coli chromosome, was further analyzed. Expression of the genes, which is involved in maltose metabolism, malQ (amylomaltase), was increased to 5.8-fold in the presence of a plasmid, pAP5, containing the subcloned sfs4 gene. The nucleotide sequence of a common 2,126bp segment of the pPCM1 was determined and two open reading frames (ORF1 and ORF2) were detected. The ORF1 encodes the sfs4 gene and ORF2 encodes a truncated protein. Potential CRP binding site is located in the upstream of the putative promoter in the regulatory region. Expression of the cloned sfs4 gene was positively regulated by the cAMP-CRP complex.

4극 이방성 Sr페라이트·플라스틱자석의 제조에 관한 연구

최희태,문현욱,신용진,진성빈 明知大學校 産業技術硏究所 1995 産業技術硏究所論文集 Vol.14 No.-

This thesis deals with the fabrication of 4 poles anisotropic Sr ferrite plastic magnets. After Polyamide6 and polyamide12 are kneaded respectively with Sr ferrite powder, silane coupling and calcium stearate of lwt% are added for coating and pelleting. The pelleted specimen injection-moulded under magnetic field using 4 poles mould. In the case of using polyamide6 as a binder, for 4 poles anisotropic Sr ferrite plastic magnets the surface magnetic flux density distribution is +943.8∼-943.8kG and the deviation is 5.2%, polyamide12, the distribution is +1040.9∼-1040.9KG and the deviation is 5.4%.The magnet distribution shows stability. As the results of experiments, we find 4 poles anistropic Sr ferrite plastic magnets have properties as the materials appropriate for manufacturing magnet type synchronous motors.

하악 제3대구치와 하악관과의 위치관계에 대한 파노라마 방사선사진과 cone beam형 전산화단층영상의 비교

최형수,김규태,최용석,황의환 대한구강악안면방사선학회 2008 Imaging Science in Dentistry Vol.38 No.3

Purpose : To assess the diagnostic accuracy and value in an imaging technique field through the comparison of cone beam computed tomography and conventional panoramic radiography in assessing the topographic relationship between the mandibular canal and impacted third molars. Materials and Methods : Participants consisted of 100 patients offered the images through cone beam computed tomography and panoramic radiography. PSR-9000 Dental CT system (Asahi Roentgen Ind. Co., Ltd, Japan) was used as the unit of cone beam computed tomography. CE-II (Asahi Roentgen Ind. Co., Ltd, Japan) and Pro Max (Planmeca Oy, Finland) were used as the unit of panoramic radiography. The images obtained through panoramic radiography were classified into 3 types according to the distance between mandibular canal and root of mandibular third molar. And they were classified into 4 types according to the proximity of radiographic feature. The images obtained through cone beam computed tomography based on the classification above were classified into 4 types according to the location between the mandibular canal and the root and were analyzed. And they were classified into buccal, inferior, lingual, and between roots, according to the location between mandibular canal and root. The data were statistically analyzed and estimated by χ²-test. Results : 1. There was no statistical significance according to 3 types (type l, type ll, type lll) through CBCT. 2. The results of 4 types (type A, type B, type C, type D) through CBCT were as high prevalence of CBCT 1 in type A, CBCT 2 in type B, CBCT 3 in type C, and CBCT1 in type D and those of which showed statistical significance (P value=0.03). 3. The results according to location between mandibular canal and root through CBCT recorded each 49, 25, 17, 9 as buccal, inferior, lingual, between roots. Conclusion : When estimating the mandibular canal and the roots through the panoramic radiography, it could be difficult to drive the views of which this estimation was considerable. Thus it is required to have an accurate diagnostic approaching through CBCT that could estimate the location between mandibular canal and roots.

원자력 사고후 쌀알과 배추내 ^90Sr 및 ^137Cs 농도 예측

최용호,임광묵,황원태,이한수,이창우 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.3

원자력 사고후 시간경과에 따른 작물체내 핵종 농도를 보다 현실적으로 예측할 수 있는 방법을 수립하고 단위 건침적에 대하여 백미와 배추내 ^90Sr 및 ^137Cs의 50 년간 농도를 예측하였다. 비생육기 사고의 경우 농도는 두 핵종 모두 시간경과에 따라 서서히 감소하였고 50년 내내 ^90Sr이 ^137Cs보다 높았다. 생육기 사고의 경우 처음 1년간 농도는 비생육기 사고에 비해 대체로 ^90Sr은 최고 30 배, ^137Cs은 최고 1,000 배 정도까지 높았다. 50 년간의 누적농도는 백미에서는 비 생육기 사고의 경우 ^90Sr이, 생육기 사고의 경우 ^137Cs이 더 높았으나 배추에서는 어느 경우에나 ^90Sr이 더 높았다. 생육기 사고시 50년간의 누적농도에 대한 지배적 경로는 대체로 ^90Sr의 경우 뿌리흡수, ^137Cs의 경우 작물체 직접오염이었다. 재부유의 영향은 무시할 수 있을 정도였다. 예측 결과에 입각하여 사고 조건별로 대책의 방향을 제시하였다. A method of more realistically predicting radionuclide concentrations in crop plants varying with time after a nuclear accident was established to estimate 50 years' concentrations of ^90Sr and ^137Cs in polished rice seeds and Chinese cabbage for unit dry deposition. After non-growing season accidents, concentrations of both nuclides decreased gradually with time and ^90Sr concentrations were higher than those of ^137Cs throughout the whole period. Radionuclide concentrations in the 1st year after growing season accidents were on the whole higher than those after non-growing season accidents by factors of up to 30 for ^90Sr and up to 1,000 for ^137Cs. In polished rice seeds, the 50 years-integrated concentration was higher for ^90Sr than for ^137Cs after non-growing season accidents, whereas the opposite was true after growing season accidents. In Chinese cabbage, however, it was higher for ^90Sr than for ^137Cs after both types of the accident. Generally speaking, the dominant pathway for the integrated concentration after the growing season accident was root uptake for ^90Sr and direct plant contamination for ^137Cs. The effect of resuspension was negligible. Based on the predicted results, the direction of planning countermeasures was suggested for various accident conditions.