http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
IDH1 mutation of gliomas with long-term survival analysis
MYUNG, JAE KYUNG,CHO, HWA JIN,PARK, CHUL-KEE,KIM, SEUNG-KI,PHI, JI HOON,PARK, SUNG-HYE Spandidos Publications 2012 ONCOLOGY REPORTS Vol.28 No.5
<P>A recurrent mutation affecting codon 132 of the isocitrate dehydrogenase 1 (IDH1) gene has been found in ~5% of primary glioblastomas (GBMs), but in >70% of secondary GBMs or oligodendroglial and astrocytic tumors. We investigated IDH1 mutations in a series of 134 brain tumors to determine the prevalence and prognostic impact of IDH1 mutations. We also examined the correlations among histology, p53 and PTEN immunoexpression, MGMT methylation status, 1p 19q co-deletion and EGFR gene amplification. The 134 brain tumors included 41 low-grade oligodendrogliomas (LOs), 47 anaplastic oligodendrogliomas (AOs) and 46 primary GBMs. Data showed that 53.7% (72/134) of cases showed mutations affecting codon 132 of IDH1, including 73.2% of LOs, 82.9% of AOs and three primary GBMs (6.5%). All IDH1 mutations were Arg132His. In a survival analysis, patients with IDH1 mutations had better survival compared to those with wild-type IDH1 (p<0.05) in LOs and AOs, but not in primary GBMs (p=0.587). In addition, in patients with both IDH1 mutation and MGMT methylation, p53 overexpression was a significant poor prognostic factor both in LOs and AOs. However, IDH1 mutation was not correlated with common genetic profiles that affect patient prognosis, including MGMT methylation, 1p 19q co-deletion, PTEN loss and EGFR amplification in LOs, AOs and GBMs. From our results, IDH1 mutation was an independent positive prognostic factor in LOs and AOs, especially in the absence of p53 overexpression.</P>
Two Unrecoreded Species Belonging to Penicillium Section Exilicaulis in South Korea
( Myung Soo Park ),( Sung Hyun Kim ),( Jun Won Lee ),( Ji Seon Kim ),( Yoonhee Cho ),( Young Woon Lim ) 한국균학회 2020 韓國菌學會誌 Vol.48 No.3
Penicillium in section Exilicaulis is characterized by non-vesiculate monoverticillate and biverticillate stipes. Species in sect. Exilicaulis are commonly found in soil and plants in terrestrial environments; however, only a few species have been reported in Korea. To investigate the diversity of Penicillium sect. Exilicaulis, Penicillium species were isolated from terrestrial and marine environments. Based on sequence analyses of β-tubulin, calmodulin, and the second largest subunit of RNA polymerase II loci, 19 strains of Penicillium in sect. Exilicaulis were identified as P. citreonigrum, P. citreosulfuratum, P. corylophilum, P. menonorum, P. rubefaciens, P. velutinum, Penicillium sp. 1, and Penicillium sp. 2. Two of them, P. citreonigrum and P. citreosulfuratum, were confirmed to be new to Korea. Molecular phylogenies and detailed descriptions of the two unrecorded species are provided.
<sup>18</sup>F-flortaucipir uptake patterns in clinical subtypes of primary progressive aphasia
Cho, Hanna,Kim, Hee Jin,Choi, Jae Yong,Ryu, Young Hoon,Lee, Myung Sik,Na, Duk L.,Seo, Sang Won,Lyoo, Chul Hyoung Elsevier 2019 Neurobiology of aging Vol.75 No.-
<P><B>Abstract</B></P> <P>We analyzed <SUP>18</SUP>F-flortaucipir uptake patterns and structural changes in patients with subtypes of primary progressive aphasia (PPA) using <SUP>18</SUP>F-flortaucipir positron emission tomography and volumetric magnetic resonance imaging. We enrolled 34 consecutive patients with PPA (10 nonfluent/agrammatic PPA [nfvPPA], 18 semantic variant PPA [svPPA], and 6 logopenic variant PPA [lvPPA], as well as 20 healthy controls, and 20 patients with Alzheimer's disease. <SUP>18</SUP>F-flortaucipir uptake was increased in the frontal cortex and underlying white matter, and subcortical nuclei in the 10 nfvPPA and 8 nfvPPA-amyloid-β (Aβ)− subgroup patients. In the svPPA patients (both the 13 svPPA-Aβ− and 5 svPPA-Aβ+), uptake generally increased in the widespread neocortex with left anterior temporal predominance. <SUP>18</SUP>F-flortaucipir uptake patterns in the 6 lvPPA and the 5 lvPPA-Aβ+ subgroup patients were similar to those seen in the patients with Alzheimer's disease with mild predominance in the left lateral temporal cortex. Cortical thinning in each PPA subtype corresponded with increased <SUP>18</SUP>F-flortaucipir uptake. <SUP>18</SUP>F-flortaucipir uptake patterns and cortical atrophy were distinct and corresponded to areas related to the specific language functions that are impaired in each subtype of PPA.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We compared <SUP>18</SUP>F-flortaucipir uptake and structural changes in PPA subtypes. </LI> <LI> Increased <SUP>18</SUP>F-flortaucipir uptake corresponds to areas related to language functions. </LI> <LI> Cortical thinning in each PPA subtype corresponded to increased <SUP>18</SUP>F-flortaucipir uptake. </LI> <LI> <SUP>18</SUP>F-flortaucipir uptake and cortical atrophy were distinct in each subtype of PPA. </LI> </UL> </P>
( Myung Woo Choi ),( Jong Hwa Lee ),( Sung Sik Oh ),( Mi Rim Choi ),( Hyun Ju Yang ),( Moon Sik Park ),( Jae Un Lee ),( Shang Hoon Han ),( Young Jae Lee ),( Yong Keun Cho ),( Ji Woong Kim ),( Ji Woong 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Introduction: There are various tumorous lesions in pancreas, but it is diffi cult to differenciate these lesions because of the anatomical and clinical characteristics. Through endoscopic-ultrasound(EUS)-guided biopsy, the effort for increasing diagnostic rate is still ongoing. However, Sometimes it is diffi cult to get enough tissue through EUS and to get accurate diagnostic imaging of tumorous lesion. In that case, the defi nitive diagnosis is only obtained after surgical intervention. Authors report one case, which was diagnosed as the pancreatic cancer before the operation and had a pancreaticoduodenectomy, but the lesion was proved to be tumor forming chronic pancreatitis on histological examination of a frozen-section. Case: A 65-years-old male patient presented with indigestion for about 1week and he was found to have a tumorous lesion in pancreas by abdominal-ultrasonography at local. He was a chronic drinker who consumed 100g of alcohol everyday. On initial laboratory evaluation, PT-1. 02, AST/ALT-22/12U/L, ALP-342IU/L, T-bilirubin-0. 2mg/ dL, Amylase-74U/L, lipase-160U/L, CA19-9-9. 13U/mL, CEA-6. 1ng/mL. In Contrast-enhanced abdominal-CT, 6. 4x5. 0x4. 0cm mass was found in the pancreas head, which was low density and little more enhanced than normal pancreas and the boundary was relatively clear. EUS-guided Procore 25G-needle biopsy and Cytology was done, but only normal pancreas tissues were found. However there was FDG uptake (SUVmax- 3. 79) in the head of pancreas on PET-CT. So we diagnosed this case as pancreas- cancer in T3N1 and he underwent pancreaticoduodenectomy. After the operation, fi nal histological diagnosis was mass forming chronic pancreatitis. Conclusion: Chronic pancreatitis can present with a fi brotic mass. If there is a risk factor like chronic alcohol intake in medical history taking and CA 19-9 is normal, it could be the chronic pancreatitis which has a possibility of forming mass rather than pancreatic cancer. In conclusion, it needs more effort of imaging examination and tissue confi rmation for accurate diagnosis.
Sung-Sik Kang,Sang-Rae Cho,Ui-Hyung Kim,Seok-Dong Lee,Myung-Suk Lee,Boh-Suk Yang 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
Artificial insemination with frozen-thawed epdidymal sperm showed low motility and conception rate compared to those with ejaculated frozen-thawed sperm. Cauda epididymal sperm has not contained seminal plasma. We hypothesized that addition of semenial plasma will affects epididymal sperm motility. Therefore, we examined the effect of different seminal plasma concentrations on frozen-thawed epididymal sperm motility. Two testicles were recovered by castration from one Hanwoo bull (age=441 days, scrotal circumference (cm)=31, weight (kg)=420). Epididymis with vas deferens in each bull was isolated from testis and blood vessels on surface of cauda epididymis. Cauda epididymal sperm were recovered by flushing and floating methods with semen freezing medium (OptixCell, IMV, France). Sperm concentration adjusted to 40×106 cells/mL, semen dilution loaded to 0.5 mL straw and crypreserved in LN2 tank until use. Seminal plasma was collected from a Hanwoo bull. In brief, semen collected by artificial vagina and centrifuged at 5,000 ×g for 10 min. The supernatant recovered and centrifuged 5,000 ×g for 10 min for 30 min. The supernatant was cryopreserved at − 80°C until use. Six straws of frozen-semen were thawed at 37°C for 40 sec, mixed and divided into 5 groups (500 uL/group). Frozen-thawed seminal plasma added to prepared 5 frozen-thawed epididymal sperm groups with 0, 1, 2, 5 and 10% of seminal plasma. Sperm motility was evaluated at 0, 2, 4, 6 and 8 h of incubation periods using a computer assisted sperm analysis system (MicroOptic, Spain). Five replicates in each groups were conducted. Addition of 2, 5 and 10% of seminal plasma to frozen- thawed epididymal sperm improved VSL (μM/sec), VAP (μM/sec), LIN (%) and decreased BCF (Hz) compared to those of 0% of seminal plasma group at 4 to 8 h of incubation periods. In conclusion, addition of seminal plasma to frozen-thawed epididymal sperm will improve longevity of sperm in vitro. It suggested that artificial insemination with epididymal sperm and seminal plasma will improve conception rate in vivo.
Cho, Myung-Yeon,Lee, Dong-Won,Kim, Ik-Soo,Kim, Won-Jung,Koo, Sang-Mo,Lee, Daeseok,Kim, Yoon-Hyun,Oh, Jong-Min Elsevier 2019 CERAMICS INTERNATIONAL Vol.45 No.6
<P><B>Abstract</B></P> <P>Four types of bioceramic films (Al<SUB>2</SUB>O<SUB>3</SUB>, SiO<SUB>2</SUB>, hydroxyapatite (HA), and brushite) were prepared via a simple aerosol deposition (AD) process at room temperature, and their structural and mechanical characteristics were compared to verify their potential for use in orthodontic brackets. The results showed that the HA and brushite films featured considerably high deposition rates compared with other coating technologies. Moreover, it was revealed that the crystallite size of all samples was largely reduced below 100 nm, which can enhance the biological performance and mechanical properties of bioceramic films. The HA and brushite films also had optimum mechanical properties, namely, adhesive strength and shear bond strength. These two instrumental mechanical properties were believed to be achieved by not only thicker bumps due to the high deposition rate but also strong mechanical interlocking at the ceramic-to-resin interface. This study confirmed that AD-prepared HA and brushite films have high potential for application in orthodontic brackets.</P>