Relationship between Stress Gene Polymorphisms and Litter Size by AI in Pigs

H. J. Jin,I. C. Kim,M. S. Wee,S. H. Yeon,C. D. Kim,S. S. Lee,C. Y. Cho,S. R. Cho,D. S. Son,C. K. Park,Z. D. Li 사단법인 한국동물생명공학회 2007 한국동물생명공학회지 Vol.22 No.4

This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire(p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.

VP2 capsid domain of the H-1 parvovirus determines susceptibility of human cancer cells to H-1 viral infection

Cho, I-R,Kaowinn, S,Song, J,Kim, S,Koh, S S,Kang, H-Y,Ha, N-C,Lee, K H,Jun, H-S,Chung, Y-H Nature America, Inc. 2015 Cancer gene therapy Vol.22 No.5

Although H-1 parvovirus is used as an antitumor agent, not much is known about the relationship between its specific tropism and oncolytic activity. We hypothesize that VP2, a major capsid protein of H-1 virus, determines H-1-specific tropism. To assess this, we constructed chimeric H-1 viruses expressing Kilham rat virus (KRV) capsid proteins, in their complete or partial forms. Chimeric H-1 viruses (CH1, CH2 and CH3) containing the whole KRV VP2 domain could not induce cytolysis in HeLa, A549 and Panc-1 cells. However, the other chimeric H-1 viruses (CH4 and CH5) expressing a partial KRV VP2 domain induced cytolysis. Additionally, the significant cytopathic effect caused by CH4 and CH5 infection in HeLa cells resulted from preferential viral amplification via DNA replication, RNA transcription and protein synthesis. Modeling of VP2 capsid protein showed that two variable regions (VRs) (VR0 and VR2) of H-1 VP2 protein protrude outward, because of the insertion of extra amino-acid residues, as compared with those of KRV VP2 protein. This might explain the precedence of H-1 VP2 protein over KRV in determining oncolytic activity in human cancer cells. Taking these results together, we propose that the VP2 protein of oncolytic H-1 parvovirus determines its specific tropism in human cancer cells.

Relationship between Stress Gene Polymorphisms and Litter Size by AI in Pigs

Jin, H.J.,Kim, I.C.,Wee, M.S.,Yeon, S.H.,Kim, C.D.,Lee, S.S.,Cho, C.Y.,Cho, S.R.,Son, D.S.,Park, C.K. 韓國受精卵移植學會 2007 한국동물생명공학회지 Vol.22 No.4

This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire (p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.

Antiviral phenolics from the leaves of Cleistocalyx operculatus

Ha, T.K.Q.,Dao, T.T.,Nguyen, N.H.,Kim, J.,Kim, E.,Cho, T.Oh.,Oh, W.K. Elsevier 2016 Fitoterapia Vol.110 No.-

<P>During the screening program for anti-influenza agents from medicinal plants, the ethanolic extract of Cleistocalyx operculatus leaves was found to exhibit potential neuraminidase (NA) inhibitory activity. Bioassay directed fractionation led to the isolation of two new acetophenones (1 and 2) and one new flavanone (3), along with six known compounds (4-9). The structures of all isolated compounds were elucidated using various spectroscopic methods and through comparison with the previous literature. Compounds 6 and 8 exhibited strong enzymatic inhibition on various neuraminidases from different influenza viruses, including H1N1, H9N2, novel H1N1, and oseltamivir-resistant novel H1N1 (H274Y mutation) expressed in HEM293 cells (IC50 values ranging from 5.07 +/- 0.94 mu M to 9.34 +/- 2.52 mu M, respectively). Kinetic experiments revealed the non-competitive inhibitory mode of both compounds 6 and 8. Furthermore, these flavonoids reduced the cytopathic effect of the H1N1 virus in MDCK cells. The present study suggests the potential of two flavonoids (6 and 8) as new lead compounds for the development of novel NA inhibitors in the future. (C) 2016 Elsevier B.V. All rights reserved.</P>

The Effects of Stress Related Genes on Carcass Traits and Meat Quality in Pigs

H. J. Jin,B. Y. Park,J. C. Park,I. H. Hwang,S. S. Lee,S. H. Yeon,C. D. Kim,C. Y. Cho,Y. K. Kim,K. S. Min,S. T. Feng,Z. D. Li,C. K. Park,C. I. Kim 아세아·태평양축산학회 2006 Animal Bioscience Vol.19 No.2

The current study was conducted to investigate the relationship between stress related gene and meat quality in pigs. A total number of 212 three-way cross bred (Landrace-Yorkshire횞Duroc) and 38 Duroc were sampled from the Korean pig industry to determine genotype requency of porcine stress syndrome (PSS) and heat shock protein 70 kDa (HSP70) genes and their relationship with carcass traits and longissimus meat quality. Screen of HSP70 was performed by the single strand conformation polymorphism (SSCP) technique. Based on the analysis of restriction fragment length polymorphism (RFLP) in ryanodine receptor 1 (RYR1) gene, genetic disorder of PSS was related to a mutation at 18,168th (C to T) of exon 17. There was no significant difference in ultimate meat pH and backfat thickness between HSP70 K1-AA type and -BB type in pure Duroc breed. In Landrace-Yorkshire횞Duroc (L-YxD) cross bred pig, our results indicated that HSP70 derivate type in Duroc had a limited effect on backfat thickness, but L-YxD type had a noticeable linkage with HSP70 K1-AA and K3-AB. This tendency was also observed in hot carcass weight where HSP70 K1-AA and K3-AB resulted in heavier weight with 86.3 kg compared to HSP70 K1-AB and K3-BB of 74.3 kg. Results imply that stress related HSP70 genotype has a potential association with backfat thickness and carcass weight.

Attenuation of oxidative neuronal cell death by coffee phenolic phytochemicals

Cho, E.S.,Jang, Y.J.,Hwang, M.K.,Kang, N.J.,Lee, K.W.,Lee, H.J. Elsevier 2009 Mutation research Vol.661 No.1

Neurodegenerative disorders such as Alzheimer's disease (AD) are strongly associated with oxidative stress, which is induced by reactive oxygen species (ROS) including hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>). Recent studies suggest that moderate coffee consumption may reduce the risk of neurodegenerative diseases such as AD, but the molecular mechanisms underlying this effect remain to be clarified. In this study, we investigated the protective effects of chlorogenic acid (5-O-caffeoylquinic acid; CGA), a major phenolic phytochemical found in instant decaffeinated coffee (IDC), and IDC against oxidative PC12 neuronal cell death. IDC (1 and 5μg/ml) or CGA (1 and 5μM) attenuated H<SUB>2</SUB>O<SUB>2</SUB>-induced PC12 cell death. H<SUB>2</SUB>O<SUB>2</SUB>-induced nuclear condensation and DNA fragmentation were strongly inhibited by pretreatment with IDC or CGA. Pretreatment with IDC or CGA also inhibited the H<SUB>2</SUB>O<SUB>2</SUB>-induced cleavage of poly(ADP-ribose) polymerase (PARP), and downregulation of Bcl-X<SUB>L</SUB> and caspase-3. The accumulation of intracellular ROS in H<SUB>2</SUB>O<SUB>2</SUB>-treated PC12 cells was dose-dependently diminished by IDC or CGA. The activation of c-Jun N-terminal protein kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) by H<SUB>2</SUB>O<SUB>2</SUB> in PC12 cells was also inhibited by IDC or CGA. Collectively, these results indicate that IDC and CGA protect PC12 cells from H<SUB>2</SUB>O<SUB>2</SUB>-induced apoptosis by blocking the accumulation of intracellular ROS and the activation of MAPKs.

자외선의 상이한 선량이 브로일러 병아리의 중족골 광물질 함량에 미치는 영향

조인호,장윤환,여영수,강훈석,김강수,배은경 한국축산학회 1994 한국축산학회지 Vol.36 No.2

This study was carried out to investigate the concentrations of Ca, P and ash in the metatarsus of broiler chicks exposed to 0.204 mJ/㎠ 30 min) or 0.409 mJ/㎠(60 min) of medium wave ultraviolet(UVB) fight. Eighty two Hubbard dayold broiler chicks(2 doses × 9 elapsed times × 4 replicate + 10 negative controls) were fed vitamin D(VD)deficient diets for 31 days in a windowless subdued light room. The metatarsus were collected at 0, 6, 12, 18, 30, 42, 66, 90, or 138 h after UVB irradiation. The metatarsus bones were separated from adhering tissue, ether extracted, dried and ashed(A.O.A.C., 1984). The Ca content in non-fat dry metatarsus was measured by atomic absorption spectrophotometry and P by urnmonium metavanadate colorimetry. The mean Ca concentration in metatarsus of negative control birds was 21 %, and those of chicks exposed to UVB light for 30 and 60 min were 30 and 38%, respectively with the maximum values at 42 h after irradiation. Thereafter, the Ca contents decreased throughout the l38 h period of this research. The mean P level of control chicks was 8.5%, and the level peaked at 15% (30 min treatment) and 11%(60 min) at 66 h after exposure. The decreasing trend continued later than 66 h. This mean ash content of the control group was 43% with the ash contents of the 0.204 mJ/㎠(30 min) and 0.409 mJ/㎠(60 min) groups decreasing slightly until 42 h, but increasing to 46%(30 min) and 47%(60 min) at l38 h after irradiation. This verified that Ca and P contents increased first and ash amount increased later in metatarsal bones of broiler chicks when they were exposed to 0.204 and 0.409 mJ/㎠ UVB light. Since there was not a big difference between minerals in metatarsal bones of chicks exposed to 0.204 or 0.409 mJ/㎠ UVB light, it was thought that further experiments would be necessary to elucidate the optimum dose of UVB light less than 0.204 mJ/㎠.

k-NN 분류를 이용한 유압유 미립자 검출 기반 연구

최병희(B. H. Choi),조예찬(Y. C. Jo),박정현(J. H. Park),최성운(S. W. Choi),조상훈(S. H. Cho),김바다(B. D. Kim),박기협(K. H. Park),김주호(J. H. Kim),이대엽(D. Lee) 유공압건설기계학회 2023 유공압건설기계학회 학술대회논문집 Vol.2023 No.5

In many equipment, including construction machinery that operates with hydraulic oil, it is very important to solve the problem of the generation of fine particles in the hydraulic circuit and the inability to use the equipment due to causes such as damage of parts and repair. In this study, as a base study to solve these problems, a study that can detect the color change of hydraulic oil due to particles mixed in hydraulic oil in real time using machine learning was conducted. Using the k-NN classification algorithm, the color change of hydraulic oil is classified into ten classes so that the color can be recognized in real time. It is planned to carry out the development of the sensor system in the following study.

자외선의 조사간격이 브로일러 병아리의 볏 피부중 비타민 D₃함량에 미치는 영향

조인호,장윤환,이은택,여영수,배은경,김중달 한국축산학회 1994 한국축산학회지 Vol.36 No.1

This study was conducted to determine the content, of previtamin D₃(PreD₃), lumisterol₃(L₃), vitamin D₃(VD₃) and provitamin D₃(ProD₃) in comb skski of broiler chicks exposed to medium ware ultraviolet(UVB) lights in different interval. The broiler Hubbard line day old chicks(199 = 10 control + 3 irradiation interval × 9 elapsed time × 7 replica) were fed vitamin D deficient diet for 3 weeks in a windowless subdued light room and exposed to 297 ㎚ UVB light by 0.068 mJ/㎝-(10 min) three times in 0, 12 or 24 h interval. The comb skin were taken at 0, 6, 12, 18, 24, 48, 96, 144 or 240 h after last irradiation, and epidermis and dermis were separated. The lipid in sample was extracted by 9% ethyl acetate/hexane and purified by Sep-Pak silica catridge. The stright phase HPI-C was applied to analyze the concentration of Prop; and its photoproducts. When chicks were exposed once to UVB light for 30 min without interval, the mole % of ProD₃ in comb epidermis were 100% at control and 52.65% at 0 h after irradiation, thereafter it increased gradually to 88.17% at 240 h. PreD₃ and L₃ presented the maximum mole % at 0 h. VD₃ showed the peak value at 12 h. then decreased slowly. As UVB light was utilized to irradiate the chicks for 10 thin three times in 12 h interval, the ProD₃ mole portion in epidermis at 0 h was 76.4%, the lowest value among tested. PreD₃ and 1-3 preserved the highest level at 24 and 0 h, respectively, thereafter decreased gradully. VD₃ showed a peak at 6 h after exposure. When 24 h interval system was treated, the lowest value of ProD₃ 83.52% was appeared at 0 h. PreD₃ and L3 showed the highest level at 6 and 0 h, respectively. Mole ale of VD₃ had a peak value at 6 h and thin decreased. The mole % of ProD₃ and its photoproduets in comb dermis presented similar trends of time course variation as in those in epidermis. In respecting the method of UVB irradiation the PreD₃, L, and VDT were produced more quickly and largely in no intend system as compared to the time and amount produced in 12 or 24 h interval system.

Cooperative Activation of PI3K by Ras and Rho Family Small GTPases

Yang, H.,Shin, M.G.,Lee, S.,Kim, J.R.,Park, W.,Cho, K.H.,Meyer, T.,Do Heo, W. Cell Press 2012 Molecular cell Vol.47 No.2

Phosphoinositide 3-kinases (PI3Ks) and Ras and Rho family small GTPases are key regulators of cell polarization, motility, and chemotaxis. They influence each other's activities by direct and indirect feedback processes that are only partially understood. Here, we show that 21 small GTPase homologs activate PI3K. Using a microscopy-based binding assay, we show that K-Ras, H-Ras, and five homologous Ras family small GTPases function upstream of PI3K by directly binding the PI3K catalytic subunit, p110. In contrast, several Rho family small GTPases activated PI3K by an indirect cooperative positive feedback that required a combination of Rac, CDC42, and RhoG small GTPase activities. Thus, a distributed network of Ras and Rho family small GTPases induces and reinforces PI3K activity, explaining past challenges to elucidate the specific relevance of different small GTPases in regulating PI3K and controlling cell polarization and chemotaxis.