Staphylococcin 감수성(感受性)에 의한 Staphylococcus hyicus subsp. hyicus의 균형별(菌型別)

박청규,Park, Cheong-kyu 대한수의학회 1993 대한수의학회지 Vol.33 No.3

Strains of Staphylococcus hyicus subsp. hyicus were compared and differentiated by staphylococcin sensitivity. Strains were tested by the cross-streaking method on brain heart infusion agar. From 31 staphylococcin producers, a typing set of 9 producers was selected and used to type all of the strains. All 81 strains of Staph hyicus subsp. hyicus tested were typable and were differentiated into 12 different sensitivity patterns. Strains of the same serotype from different sources could be further subdivided by their staphylococcin sensitivity patterns. Other staphylococcus species could also be typed by use of the 9 standard strains of Staph hyicus subsp. hyicus.

젖소 유방염유래(乳房炎由來) Gram 음성간균(陰性桿菌)의 Ampicillin 내성(耐性) 및 전달성(傳達性) β-Lactamase Plasmids

박청규,Park, Cheong-kyu 대한수의학회 1985 大韓獸醫學會誌 Vol.25 No.1

One hundred and twenty seven strains of Gram-negative rods (72 E. coli, 45 Klebsieila pneumoniae, 8 Enterobacter spp. and 2 Pseudomonas aeruginosa) isolated from bovine mastitis were examined for resistance to ampicilin, carbenicillin and cefazolin, ${\beta}$-lactamase activity and transferable ${\beta}$-lactamase plasmids. Stains resistant to ampicillin were 13.9% in E. coli, 93.3% in Klebsiella pneumoniae, 87.5% in Enterobacter. spp. and all in Pseudomonas aeruginosa, Resistance of E. coli, Klebsiella pneumoniae and Enterobacter spp. to ampicillin was due to the ${\beta}$-lactamases, but all Pseudomonas aeruginosa exhibited a high level of the non-enzymic resistance. Transferable plasmid-mediated ${\beta}$-lactamase synthesis was demonstrated in 61.9% of Klebsiella pneumoniae, 50% of E. coli and 42.9% of Enterobacter spp. The same ${\beta}$-lactamase plasmids specified different resistance levels to various ${\beta}$-lactam antibiotics in different recipients.

돼지 삼출성(渗出性) 표피염(表皮炎)에 관한 연구(硏究) : I. 발증돈(發症豚) 및 건강돈(健康豚)으로부터 Staphylococcus hyicus subsp. hyicus의 분리(分離) 및 그 성장(性狀)

박청규,강병규,Park, Cheong-kyu,Kang, Byong-kyu 대한수의학회 1986 大韓獸醫學會誌 Vol.26 No.2

Isolation of Staphylococcus hyicus subsp. hyicus from piglets suffering from exudative epidermitis and healthy pigs was performed, and some properties of the isolates were examined. Twentry-six litters of exudative epidermitis observed in the field were sucking piglets with ages ranging from 1 to 5 weeks and most (73.1%) of these cases occurred in piglets between 2 and 3 weeks of age. Staphylococcus hyicus subsp. hyicus was isolated front 192(38.9%) of 494 healthy pigs. The rate of isolation of these organism from healthy pigs was found to vary greatly among farms at an isolation rate of 33.3% to 45.5% and this organism was isolated more frequently front sucking piglets below the age of 8 weeks than adult pigs. All of the isolates originated from the diseased and healthy pigs were positive in heat-stable DNase, Tween 80 hydrolysis, gelatinase, protease, but negative in clumping factor and hemelysin on sheep blood agar. There was no difference related to origin in the production of extracellular active substances. Typical lesions of the classic exudative epidermitis were produced by inoculation of Staphylococcus hyicus subsp. hyicus isolated from the diseased and healthy pigs. The pathogenic potentials of isolates from healthy pigs were no different from that of the isolates obtained from diseased pigs.

젖소 유방유래(乳房由來) 난포구원(蘭葡球園)에 관한 연구(硏究) : III. 분리균주(分離菌株)에 대한 Penicillins 및 Gentamicin의 항균효과(抗菌效果)

박청규,Park, Cheong-kyu 대한수의학회 1984 大韓獸醫學會誌 Vol.24 No.2

The in vitro activity of penicillins(penicillin G, ampicillin, carbenicillin, methicillin and cloxacillin) and gentamicin to Staphylococcus aureus and coagulase-negative staphylococci isolated from bovine mastitic milk samples was determined. The growth of all isolates of staphylococci tested was inhibited by cloxacillin and methicillin at a concentration of $0.78{\mu}g/ml$ and by gentamicin at a minimum inhibitory concentration (MIC) of $1.56{\mu}g/ml$. Of the 140 strains of Staphylococcus aureus isolated 99(70.7%) gave positive reactions for penicillinase on the starch-iodine test. Of 121 isolates of coagulase-negative staphylococci, 58(47.9%) showed penicillinase production, but all the Staph. xylosus lacked the ability to produce penicillinase. MIC of penicillin G of the penicillinase-positive strains was $0.2{\mu}g/ml$ or more. The combination of cloxacillin with gentamicin showed a synergistic effect by inhibiting regrowth of the tested organism.

젖소 유방염유래(乳房炎由來) 포도구균(葡萄球菌)에 관한 연구(硏究) II. Coagulase음성(陰性) Staphylococci의 분류(分類) 및 생화학적(生化學的) 특성(特性)

박청규,조용준,Park, Cheong-Kyu,Cho, Yong-Joon 대한수의학회 1983 大韓獸醫學會誌 Vol.23 No.2

The distribution of slide coagulase-negative staphylococci isolated from bovine mastitic milk samples was investigated mainly according to Kloos and Schleifer's classification scheme, and toxigenic and enzymatic characteristics of these strains were also examined. One-hundred-and-twenty-one strains of coagulase-negative staphylococci isolated were classified into 8 species. Of these species, Staphylococcus epidermidis, Staph. xylosus, Staph. haemolyticus and Staph. simulans were more frequently found in bovine mastitic milk samples, and toxin and enzyme production of these species were observed in relatively high frequency. Staph. hyicus subsp. hyicus was isolated from the 4 quarters with clinical mastitis included in this investigation. By the use of Baird-Parker and Pelzer's classification system, 44.6% and 18.2% of the strains could not be classified in any subgroup, respectively.

돼지 삼출성표피염에 관한 연구(硏究) : III. Staphylococcus hyicus subsp. hyicus의 항균제(抗菌劑)에 대한 감수성(感受性)

박청규,강병규,Park, Cheong-kyu,Kang, Byong-kyu 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.2

A total of 204 strains of Staphylococcus hyicus subsp. hyicus isolated from piglets suffering from exudative epidermitis and healthy pigs were tested for antibiotic susceptibility. The growth of all isolates of Staph. hyicus subsp. hyicus tested was inhibited by cloxacillin(Cx) at a concentration of $0.8{\mu}g/ml$ and by cefazolin (Cf) at a concentration of $0.4{\mu}g/ml$, and by gentamicin and methicillin at a minimum inhibitory concentration of $3.2{\mu}g/ml$. Of total isolates obtained from the diseased and healthy pigs, 69.6% were resistant to penicillin G, 53.4% to tetracycline, 11.8% to kanamycin and 0.5% to chloramphenicol, with considerable variations among farms in the occurrence of resistant strains and resistance patterns to the drugs. By application of Cx or Cf which was active to the organism, piglets exposed to infection were successfully protected from the disease, but piglets suffering from already severe exudative epidermitis did not respond to treatment.

API STAPH-IDENT system에 의한 돼지 및 닭유래(由來) staphylococcus hyicus subsp hyicus의 동정(同定)

박청규,Park, Cheong-kyu 대한수의학회 1994 대한수의학회지 Vol.34 No.2

The API STAPH-IDENT system was evaluated as a means for identifying Staphylococcus hyicus subsp hyicus straints isolated from swine and poultry. Of 80 strains from swine, 68 (85%) were correctly identified by the API STAPH-IDENT system alone after 5 h of incubation. When results were determined after 24 h of incubation, the accuracy of this system alone was 93.8%. By additional tests in conjunction with the API STAPH-IDENT system, however, all 80 strains could be correctly identified. Of 120 strains from poultry, 87 (72.5%) required additional testing to achieve a correct identification, and 33 (27.5%) were incorrectly identified by this system after 5 h of incubation. After 24 h of incubation, 99 of 120 (82.5%) avian strains were incorrectly identified as Staph epidermidis owing to false-negative mannose and trehalose utilizations. Seventy-seven (96.3%) of swine strains were positive for ${\beta}-glucuronidase$, whereas all 120 strains recovered tram poultry were negative for it.

젖소 유방염유래(乳房炎由來) 장내세균(腸內細菌)의 약제내성(藥劑耐性) 및 R. Plasmids

박청규,Park, Cheong Kyu 대한수의학회 1981 大韓獸醫學會誌 Vol.21 No.1

A total of 250 enteric bacteria (148 Escherichia coli, 41 Klebsiella pneumoniae, 46 Enterobacter spp. and 15 Proteus spp.) isolated from bovine udder infections in 1979 through 1980 were examined for drug resistance and prevalence of R. plasmids. The drug tested were streptomycin (SM), kanamycin (KM), ampicillin (AP), chloramphenicol (CP), tetracycline (TC), gentamicin (GM), oxolinic acid (OA) and nalidixic acid (NA). The detection of R. plasmids was performed with Escherichia coli ML 1410 NAr as the recipient. Of the 148 Escherichia coli isolated, 68(45.9%) were found to be resistant to one or more drugs tested, and about 50% of the resistant strains were multiply resistant. of the 68 drugresistant strains, 13(19.1%) were found to carry R. plasmids which were capable of performing a conjugal transfer. CP resistance was transfered together with the other resistance. Of 41 strains of Klebsiella pneumoniae isolated, 90.2% were resistant to the drugs, alone or in combination thereof. Strains resistant to AP and TC were 63.4%, and 48.8%, respectively. R. plasmids were detected in 78.4% of the drug-resistant strains, and these strains transfered all or a part of their drug resistance pattern. AP and CP resistance were transfered in 100% of AP and CP-resistant strains. Eleven (37.9%) of 29 R. plasmids showed a thermosensitive transfer. Of the 46 strains of Enterobacter spp. isolated, 37(80.4%) were resistant to the drugs tested. A high percentage of resistance was noted for AP(65.2%). All strains resistant to four or more drugs transferred their resistances to Escherichia coli ML 1410, but strains resistant to three or fewer drugs did not transfer the resistances. All of the 15 Proteus strains isolated were resistant to more than two drugs. of them, 6 were quadruple resistance to SM, KM, CP and TC, and 9 were double one to AP and TC. Three (20.0%) of the drug-resistant isolates had R.plasmids conferring AP and TC resistance. GM, OA and NA of the drugs tested were very active to all of 250 Gram-negative enteric bacteria isolated from bovine udder infections.

비육한우(肥育韓牛)에 집단발생(集團發生)한 콕시듐증(症)

박청규,장인호,Park, Cheong Kyu,Jang, In Ho 대한수의학회 1978 大韓獸醫學會誌 Vol.18 No.1

Enteritis occured in a herd of Korean native cattle in Gyeonbug district in February 1977. of 40 cattle ranging in age from 15 to 20 months, 17 had been sick for the past six days with main clinical findings of feces containing mucus and blood. Among affected cattle, feces of 8 cattle were examined bacteriologically and parasitologically to investigate the causative agent. The disease was diagnosed as bovine coccidiosis caused by Eimeria zurnii based on the detection of $o{\ddot{o}}cysts$ and one of cattle was infected E. bukidnonensis, too.

돼지 유래(由來) Staphylococcus hyicus subsp. hyicus의 Bacteriocin(Staphylococcin)형별(型別)

박청규,Park, Cheong-kyu 대한수의학회 1992 대한수의학회지 Vol.32 No.4

A total of 113 Staphylococcus hyicus subsp. hyicus which had been isolated from healthy and diseased pigs were examined for their ability to produce staphylococcins. For selection of staphylococcin indicator strains, 80 strains were randomly selected from 113 strains, and were tested as indicator and as donator. The 8 indicators were chosen and used to type all of the strains. The ability to produce staphylococcin could be demonstrated in 43 strains (38.1%) of 113 Staph hyicus subsp. hyicus. The frequency of activity was higher in diseased pig strains (44.4%) than in healthy pig strains(36.8%). 43 staphylococcin producer strains were differentiated into 10 reaction types. The inhibitory effects of the staphylococcin (N27 and R 3) on other staphylococci differed by the staphylococcal species tested. All the gram-negative species were completely resistant to the three producing strains.