Coupling membrane and Fe–Pd bimetallic nanoparticles for trichloroethene removing from water

Bangkai Li,Xi Chen,Kun Li,Chenghao Zhang,Yang He,Runhong Du,Jianzu Wang,Li Chen 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.78 No.-

A novel composite membrane with membrane pores loading acrylic acid microgels coated by Fe–Pdbimetallic nanoparticles (NPs) was prepared for the dechlorination of trichloroethene (TCE) in water. Thismembrane was prepared byfirstly immobilizing acrylic acid microgels in poly (vinylidenefluoride)(PVDF) membrane pores and then in-situ synthesis of Fe–Pd bimetallic nanoparticles (NPs). The Fe–PdNPs coupled with the porous membranes enabled dechlorination to be conducted in a cross-flow modelincluding a penetrativeflow and a tangentialflow. In such a model, a large number of dechlorinationoccurred in penetrativeflowfluid while no dechlorination occurred in tangentialflowfluid. Thus,products and reactants are always timely isolated in the cross-flow dechlorination process. Thecomposite membrane prevents Fe–Pd NPs from contamination because its nanosize surface pores stopcolloids and bovine serum albumin (BSA) from entering into the membrane interior. However, thesesmall surfaces pores cannot slow the diffusion of reactants into membrane pores, and thus make the asformedcomposite membrane also show a fast dechlorination rate in a batch reaction. All in all, TCEdechlorination by the composite membrane shows many advantages including the fast dechlorinationrate, the convenient operation, the timely isolation of products from reactants, and the ignorable extrasteps for separation of metal NPs.

Long Noncoding RNA Expression Profiling During the Neuronal Differentiation of Glial Precursor Cells from Rat Dorsal Root Ganglia

Yunfei Dai,Wei Ma,Tong Zhang,Jinwei Yang,Chenghao Zang,Kuangpin Liu,Xianbin Wang,Jiawei Wang,Zhen Wu,Xingkui Zhang,Chunyan Li,Junjun Li,Xiangpeng Wang,Jianhui Guo,Liyan Li 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.3

Long noncoding RNAs (lncRNAs) play important roles in the process of cell fate determination. However, their function and expression profiles have not yet been systematically investigated during the transdifferentiation of glial precursor cells derived from dorsal root ganglia (DRG) in the peripheral nervous system. Our results demonstrated significant differences in gene architecture and expression among the three transcript types (lncRNA, mRNA, and TUCP). Distinct differences in transcript length, exon number, and ORF length were identified between lncRNAs and mRNAs after comparative analysis of their structure and sequence conservation. We found that the upregulated lncRNAs outnumbered the downregulated lncRNAs in glial precursor cells cultured with proBDNF antiserum compared with the levels in glial precursor cells cultured without proBDNF antiserum. By a series of GO and KEGG analyses, we found that the effects of some lncRNAs on their target genes in cis were related to nerve growth factor-induced cell cycle, cell phenotype change, and neuronal differentiation. The qRT-PCR verification results of lncRNAs ENSRNOT00000091991, ENSRNOT00000087717, and LNC_000429 were mostly consistent with the sequencing results. The candidate lncRNAs may be associated with the neuronal transdifferentiation of glial precursor cells. Our study provides the first evidence for a remarkably diverse pattern of lncRNA expression during neuronal differentiation of glial precursor cells from rat DRG, and also provides a resource for lncRNA studies in the field of cell differentiation.

Association of UDP-galactose-4-epimerase with milk protein concentration in the Chinese Holstein population

Li Cong,Cai Wentao,Liu Shuli,Zhou Chenghao,Cao Mingyue,Yin Hongwei,Sun Dong-Xiao,Zhang Shengli,Loor Juan J. 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.11

Objective: An initial RNA-Sequencing study revealed that UDP-galactose-4-epimerase (GALE) was one of the most promising candidates for milk protein concentration in Chinese Holstein cattle. This enzyme catalyzes the interconversion of UDP-galactose and UDP-glucose, an important step in galactose catabolism. To further validate the genetic effect of GALE on milk protein traits, genetic variations were identified, and genotypes-phenotypes associations were performed. Methods: The entire coding region and the 5’-regulatory region (5’-UTR) of GALE were re-sequenced using pooled DNA of 17 unrelated sires. Association studies for five milk production traits were performed using a mixed linear animal model with a population encompassing 1,027 Chinese Holstein cows. Results: A total of three variants in GALE were identified, including two novel variants (g.2114 A>G and g.2037 G>A) in the 5’-UTR and one previously reported variant (g.3836 G>C) in an intron. All three single nucleotide polymorphisms (SNPs) were associated with milk yield (p<0.0001), fat yield (p = 0.0006 to <0.0001), protein yield (p = 0.0232 to <0.0001) and protein percentage (p<0.0001), while no significant associations were detected between the SNPs and fat percentage. A strong linkage disequilibrium (D’ = 0.96 to 1.00) was observed among all three SNPs, and a 5 Kb haplotype block involving three main haplotypes with GAG, AGC, and AGG was formed. The results of haplotype association analyses were consistent with the results of single locus association analysis (p<0.0001). The phenotypic variance ratio above 3.00% was observed for milk protein yield that was explained by SNP-g.3836G >C. Conclusion: Overall, our findings provided new insights into the polymorphic variations in bovine GALE gene and their associations with milk protein concentration. The data indicate their potential uses for marker-assisted breeding or genetic selection schemes.

A Novel Self-Learning Filters for Automatic Modulation Classification Based on Deep Residual Shrinking Networks

Ming Li,Xiaolin Zhang,Rongchen Sun,Zengmao Chen,Chenghao Liu 한국인터넷정보학회 2023 KSII Transactions on Internet and Information Syst Vol.17 No.6

Automatic modulation classification is a critical algorithm for non-cooperative communication systems. This paper addresses the challenging problem of closed-set and open-set signal modulation classification in complex channels. We propose a novel approach that incorporates a self-learning filter and center-loss in Deep Residual Shrinking Networks (DRSN) for closed-set modulation classification, and the Opendistance method for open-set modulation classification. Our approach achieves better performance than existing methods in both closed-set and open-set recognition. In closed-set recognition, the self-learning filter and center-loss combination improves recognition performance, with a maximum accuracy of over 92.18%. In open-set recognition, the use of a self-learning filter and center-loss provide an effective feature vector for open-set recognition, and the Opendistance method outperforms SoftMax and OpenMax in F1 scores and mean average accuracy under high openness. Overall, our proposed approach demonstrates promising results for automatic modulation classification, providing better performance in non-cooperative communication systems.

Domain Knowledge Actively Recommendation System Based by Process-Driven and Rough Set

XinGang Wang,ChengHao Li,Tao Sun 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.5

Recommend knowledge to staff who need in the work can improve work efficiency, knowledge application and innovation. Enterprise knowledge is described in three dimensions: knowledge attribute, process and domain. Based on this, domain knowledge actively recommendation architecture based by process-driven and rough set is construct, while domain knowledge and rough set active recommendation method is proposed. We will use this architecture to analyze employee, domain, and process. Then will using rough set to analyze the rules from logs of using knowledge. Combination with requirement of employees, process data and the rules to achieve recommend the accurate knowledge.

Identification of genes involved in inbreeding depression of reproduction in Langshan chickens

Xue Qian,Li Guohui,Cao Yuxia,Yin Jianmei,Zhu Yunfen,Zhang Huiyong,Zhou Chenghao,Shen Haiyu,Dou Xinhong,Su Yijun,Wang Kehua,Zou Jianmin,Han Wei 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.6

Objective: Inbreeding depression of reproduction is a major concern in the conservation of native chicken genetic resources. Here, based on the successful development of strongly inbred (Sinb) and weakly inbred (Winb) Langshan chickens, we aimed to evaluate inbreeding effects on reproductive traits and identify candidate genes involved in inbreeding depression of reproduction in Langshan chickens. Methods: A two-sample t-test was performed to estimate the differences in phenotypic values of reproductive traits between Sinb and Winb chicken groups. Three healthy chickens with reproductive trait values around the group mean values were selected from each of the groups. Differences in ovarian and hypothalamus transcriptomes between the two groups of chickens were analyzed by RNA sequencing (RNA-Seq). Results: The Sinb chicken group showed an obvious inbreeding depression in reproduction, especially for traits of age at the first egg and egg number at 300 days (p<0.01). Furthermore, 68 and 618 differentially expressed genes (DEGs) were obtained in the hypothalamus and ovary between the two chicken groups, respectively. In the hypothalamus, DEGs were mainly enriched in the pathways related to vitamin metabolism, signal transduction and development of the reproductive system, such as the riboflavin metabolism, Wnt signaling pathway, extracellular matrix-receptor interaction and focal adhesion pathways, including stimulated by retinoic acid 6, serpin family F member 1, secreted frizzled related protein 2, Wnt family member 6, and frizzled class receptor 4 genes. In the ovary, DEGs were significantly enriched in pathways associated with basic metabolism, including amino acid metabolism, oxidative phosphorylation, and glycosaminoglycan degradation. A series of key DEGs involved in folate biosynthesis (gamma-glutamyl hydrolase, guanosine triphosphate cyclohydrolase 1), oocyte meiosis and ovarian function (cytoplasmic polyadenylation element binding protein 1, structural maintenance of chromosomes 1B, and speedy/RINGO cell cycle regulator family member A), spermatogenesis and male fertility (prostaglandin D2 synthase 21 kDa), Mov10 RISC complex RNA helicase like 1, and deuterosome assembly protein 1) were identified, and these may play important roles in inbreeding depression in reproduction. Conclusion: The results improve our understanding of the regulatory mechanisms underlying inbreeding depression in chicken reproduction and provide a theoretical basis for the conservation of species resources. Objective: Inbreeding depression of reproduction is a major concern in the conservation of native chicken genetic resources. Here, based on the successful development of strongly inbred (Sinb) and weakly inbred (Winb) Langshan chickens, we aimed to evaluate inbreeding effects on reproductive traits and identify candidate genes involved in inbreeding depression of reproduction in Langshan chickens.Methods: A two-sample t-test was performed to estimate the differences in phenotypic values of reproductive traits between Sinb and Winb chicken groups. Three healthy chickens with reproductive trait values around the group mean values were selected from each of the groups. Differences in ovarian and hypothalamus transcriptomes between the two groups of chickens were analyzed by RNA sequencing (RNA-Seq).Results: The Sinb chicken group showed an obvious inbreeding depression in reproduction, especially for traits of age at the first egg and egg number at 300 days (p<0.01). Furthermore, 68 and 618 differentially expressed genes (DEGs) were obtained in the hypothalamus and ovary between the two chicken groups, respectively. In the hypothalamus, DEGs were mainly enriched in the pathways related to vitamin metabolism, signal transduction and development of the reproductive system, such as the riboflavin metabolism, Wnt signaling pathway, extracellular matrix-receptor interaction and focal adhesion pathways, including stimulated by retinoic acid 6, serpin family F member 1, secreted frizzled related protein 2, Wnt family member 6, and frizzled class receptor 4 genes. In the ovary, DEGs were significantly enriched in pathways associated with basic metabolism, including amino acid metabolism, oxidative phosphorylation, and glycosaminoglycan degradation. A series of key DEGs involved in folate biosynthesis (gamma-glutamyl hydrolase, guanosine triphosphate cyclohydrolase 1), oocyte meiosis and ovarian function (cytoplasmic polyadenylation element binding protein 1, structural maintenance of chromosomes 1B, and speedy/RINGO cell cycle regulator family member A), spermatogenesis and male fertility (prostaglandin D2 synthase 21 kDa), Mov10 RISC complex RNA helicase like 1, and deuterosome assembly protein 1) were identified, and these may play important roles in inbreeding depression in reproduction.Conclusion: The results improve our understanding of the regulatory mechanisms underlying inbreeding depression in chicken reproduction and provide a theoretical basis for the conservation of species resources.

Multiple Faults Detection and Isolation via Decentralized Sliding Mode Observer for Reconfigurable Manipulator

Zhao, Bo,Li, Chenghao,Ma, Tianhao,Li, Yuanchun The Korean Institute of Electrical Engineers 2015 Journal of Electrical Engineering & Technology Vol.10 No.6

This paper considers a decentralized multiple faults detection and isolation (FDI) scheme for reconfigurable manipulators. Inspired by their modularization property, a global sliding mode (GSM) based stable adaptive fuzzy decentralized controller is investigated for the system in fault free, while for the system suffering from multiple faults (actuator fault and sensor fault), the decentralized sliding mode observer (DSMO) is employed to detect their occurrence. Hereafter, the time and location of faults can be determined by a fault isolation scheme via a bank of DSMOs. Finally, the effectiveness of the proposed schemes in controlling, detecting and isolating faults is illustrated by the simulations of two 3-DOF reconfigurable manipulators with different configurations successfully.

Multiple Faults Detection and Isolation via Decentralized Sliding Mode Observer for Reconfigurable Manipulator

Bo Zhao,Chenghao Li,Tianhao Ma,Yuanchun Li 대한전기학회 2015 Journal of Electrical Engineering & Technology Vol.10 No.6

This paper considers a decentralized multiple faults detection and isolation (FDI) scheme for reconfigurable manipulators. Inspired by their modularization property, a global sliding mode (GSM) based stable adaptive fuzzy decentralized controller is investigated for the system in fault free, while for the system suffering from multiple faults (actuator fault and sensor fault), the decentralized sliding mode observer (DSMO) is employed to detect their occurrence. Hereafter, the time and location of faults can be determined by a fault isolation scheme via a bank of DSMOs. Finally, the effectiveness of the proposed schemes in controlling, detecting and isolating faults is illustrated by the simulations of two 3-DOF reconfigurable manipulators with different configurations successfully.

Sperm maturation screening and the effect of ecdysone on sperm development of silkworm Bombyx mori

Cheng Hao,Wang Yanyan,Chang Meiling,Zhang Kun,Cai Zhiyan,Qian Yuxin,Li Dandan 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2

There are two sperm morphs of silkworm, the nucleated spermatozoa (eupyrene) and anucleated spermatozoa (apyrene). Eupyrene sperm cannot complete fertilization successfully without the apyrene sperm. Here a modified rapid and efficient method for sperm identification was developed, after 10 s of fixation in para formaldehyde and 30 s of 4 ′ 6-diamidino-2-phenylindole (DAPI) or propidium Iodide (PI) staining, the sperm bundles can be detected easily using a fluorescence microscope. Sperm maturation process of silkworm from the fifth instar larvae to the adult was described with the above method, the precise time of earliest elongate apyrene bundles was detected on day 2 of pre-pupation, with a ratio of 5% in total sperm bundles, after which the percentage of apyrene sperm bundles increased rapidly and attained a relatively stable ratio of 75% at the end of pupation and nearly 80% after eclosion. Delayed mating leads to apyrene sperm accumulation and damaged fertilization. Previous study showed that ecdysone can increase the frequency of apyrene sperm bundles in vitro. Here 20-hydroxyecdysone (20E) was injected into hemolymph of the 2-d-old fifth instar larvae, the worms entered into mounting period after three days injection, but no apyrene sperm bundles were induced unless day 2 of pre-pupation. Interestingly, maturation of eupyrene sperm bundles were accelerated, and the ratio of eupyrene sperm bundles increased and exhibited a dose-dependent effect after 20E injection, which indicated that the development of eupyrene sperm can be accelerated by ecdysone before pupation of silkworm in vivo. These results will provide new clues for lepidopteran pest control.