Comprehensive analysis of miRNAs, lncRNAs and mRNAs profiles in backfat tissue between Daweizi and Yorkshire pigs

Chen Chen,Yitong Chang,Yuan Deng,Qingming Cui,Yingying Liu,Huali Li,Huibo Ren,Ji Zhu,Qi Liu,Yinglin Peng Asian Australasian Association of Animal Productio 2023 Animal Bioscience Vol.36 No.3

Objective: Daweizi (DWZ) is a famous indigenous pig breed in China and characterized by tender meat and high fat percentage. However, the expression profiles and functions of transcripts in DWZ pigs is still in infancy. The object of this study was to depict the transcript profiles in DWZ pigs and screen the potential pathway influence adipogenesis and fat deposition, Methods: Histological analysis of backfat tissue was firstly performed between DWZ and lean-type Yorkshire pigs, and then RNA sequencing technology was utilized to explore miRNAs, lncRNAs and mRNAs profiles in backfat tissue. 18 differentially expressed (DE) transcripts were randomly selected for quantitative real-time polymerase chain reaction (QPCR) to validate the reliability of the sequencing results. Finally, gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis were conducted to investigate the potential pathways influence adipocyte differentiation, adipogenesis and lipid metabolism, and a schematic model was further proposed. Results: A total of 1,625 differentially expressed transcripts were identified in DWZ pigs, including 27 upregulated and 45 downregulated miRNAs, 64 upregulated and 119 down-regulated lncRNA, 814 upregulated and 556 downregulated mRNAs. QPCR analysis exhibited strong consistency with the sequencing data. GO and KEGG analysis elucidated that the differentially expressed transcripts were mainly associated with cell growth and death, signal transduction, peroxisome proliferator-activated receptors (PPAR), AMP-activated protein kinase (AMPK), PI3K-Akt, adipocytokine and foxo signaling pathways, all of which are strongly involved in cell development, lipid metabolism and adipogenesis. Further analysis indicated that the BGIR9823_87926/miR-194a-5p/AQP7 network may be effective in the process of adipocyte differentiation or adipogenesis. Conclusion: Our study provides comprehensive insights into the regulatory network of backfat deposition and lipid metabolism in pigs from the point of view of miRNAs, lncRNAs and mRNAs.

The Throughput Order of Multicast Traffics with Physical-Layer Network Coding in Random Wireless Ad Hoc Networks

Chen, Chen,Bai, Lin,He, Jianhua,Xiang, Haige,Choi, Jin-Ho The Korea Institute of Information and Commucation 2011 Journal of communications and networks Vol.13 No.3

This paper attempts to address the effectiveness of physical-layer network coding (PNC) on the throughput improvement for multi-hop multicast in random wireless ad hoc networks (WAHNs). We prove that the per session throughput order with PNC is tightly bounded as ${\Theta}((n\sqrt{m}R(n))^{-1})$ if $m=(R^{-2}(n))$, where n is the total number of nodes, R(n) is the communication range, and m is the number of destinations for each multicast session. We also show that per-session throughput order with PNC is tight bounded as ${\Theta}(n^{-1})$, when $m={\Omega}(R^{-2}(n))$. The results of this paper imply that PNC cannot improve the throughput order of multicast in random WAHNs, which is different from the intuition that PNC may improve the throughput order as it allows simultaneous signal access and combination.

Neutrophil to lymphocyte ratio as a predictor of myocardial damage and cardiac dysfunction in acute coronary syndrome patients

Chen Chen,Bai Lin Cong,Min Wang,Muhammad Abdullah,Xiao Long Wang,Yin Hua Zhang,Shun Ji Xu,Lan Cui 한국한의학연구원 2018 Integrative Medicine Research Vol.7 No.2

Background: Neutrophil to lymphocyte ratio (NLR) in peripheral blood is established to correlate with the morbidity and mortality of heart disease patients. We aimed to define the severity of inflammation (NLR) by observing the association of NLR with cardiac functions or myocardial damage parameters in patients with acute myocardial infarction. Methods: Data from 715 patients who underwent percutaneous coronary intervention (PCI) within 72 hours of incidence in 2016 were analysed retrospectively. Results: The NLR ranges from 0.50 to 46 (medium ± SD, 2.76 ± 2.96) in 715 patients. NLR positively correlated with myocardial damage (NLR vs. CK-mB: p < 0.0001) but negatively correlated with myocardial function (NLR vs. EF: p < 0.0001; NLR vs. FS: p < 0.0001). Myocardial damage markers (CK, CK-mB, ASL, LDH) were significantly increased, and cardiac contractile parameters (EF and FS) were reduced at NLR > 2.76 compared to those of NLR < 2.76. ELISA analysis has shown that IL-10 was significantly increased when NLR ≥ 4.6 and TGF-β was increased at NLR > 4. The correlation was diminished between NLR and CK-mB at NLR > 2.76 or at NLR > 4, but that of NLR and EF or FS was maintained in NLR > 2.76 and at NLR > 4. EF and FS were comparable between NLR > 2.76 and NLR > 4. But myocardial damage parameters increased significantly at NLR > 4 compared to those of NLR > 2.76. Conclusion: NLR is a strong predictor of myocardial damage in acute myocardial patients. High NLR are associated with myocardial dysfunction in all the patients. Severe inflammation (NLR) can predict the consequence of the heart in patients with coronary syndrome.

An Active Damping Scheme Based on a Second Order Resonant Integrator for LCL-Type Grid-Connected Converters

Chen Chen,Jian Xiong,Kai Zhang 전력전자학회 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.4

This paper proposes a novel active damping scheme to suppress LCL-filter resonance with only grid-current feedback control in grid-connected voltage-source converters. The idea comes from the concept of the model reference adaptive control (MRAC). A detailed theoretical derivation is given, and the effectiveness of this method is explained based on its physical nature. According to the control structure of this method, the active damping compensator, which is essentially a second order resonant integrator (SORI) filter, provides an effective solution to damp LCL resonance and to eliminate the need for additional sensors. Compared with extra feedback methods, the cost and complexity are reduced. A straightforward tuning procedure for the active damping method has been presented. A stability analysis is illustrated in the discrete domain while considering a one-step delay. Finally, experimental results are presented to validate the analysis and to demonstrate the good performance of the proposed method.

An Analysis of Narrative Art of Motion Graphics in the Film Openings and Closings

Chen Chen 한중경제문화학회 2021 한중경제문화연구 Vol.16 No.-

Wireless power transfer system for angled concave conditions utilizing quasi-bowl-shaped couplers

Chen, Chen,Li, Jiangui,Wang, Longyang,Nie, Hui,Li, Qiang The Korean Institute of Power Electronics 2021 JOURNAL OF POWER ELECTRONICS Vol.21 No.7

Wireless power transfer (WPT) is favored by consumers due to its convenience, safety, and flexible layout. Circular and rectangular coils are the most common coil structures in WPT systems. However, they are not suitable for installation conditions with angled groove areas such as railways, conveyors, and so on. To fully utilize installation space and to improve transfer efficiency, this paper has proposed a quasi-bowl-shaped (QBS) magnetic coupler, which can suitably fit on angled grooves. First, a QBS coupler model was presented. Second, formulas of its spatial magnetic field density were derived. Third, the influences of the bending distance and bending angle on the coupler coefficient were analyzed. Fourth, five magnetic core types were presented and analyzed for coupler optimization. Finally, QBS WPT performances were tested on a newly built experiment platform. Experimental results show that the QBS coupler can realize a constant transfer efficiency of 92.36% at an axial distance of 6 cm, and it can realize a constant transfer efficiency of 84.17% at an axial distance of 10 cm.

Digital Transformation, Manipulation of Asset Evaluation and M&A Performance: Discussion on the Intermediary Effect of Internal Control

Chen Chen,Hee-Jung Lee,Nan Hui,Xue-Hua Qian 강원대학교 경영경제연구소 2023 Asia-Pacific Journal of Business Vol.14 No.1

Purpose - The purpose of this study was to examine the internal relevance between digital transformation, manipulation of asset evaluation and corporate M&A performance and further explores the impact path of manipulation of asset valuation on corporate M&A performance. Design/methodology/approach - This study based on the financial data of A-share listed companies in Shanghai and Shenzhen Stock Exchanges from 2013 to 2021. Findings - First, manipulation of asset evaluation is negatively correlated with M&A performance. Second, Digital transformation significantly weakens the negative correlation between manipulation of asset evaluation and corporate M&A performance. Third, The effectiveness of internal control plays a partially intermediary role in the process of manipulation of asset evaluation affecting M&A performance. Research implications or Originality - Enriching the existing literature on the subject, the study can also provide useful reference for improving the performance of corporate mergers and acquisitions, regulating asset valuation, promoting the digital transformation of enterprises and improving internal control mechanisms, with both theoretical and practical implications.

Teaching Research of Design Thinking Based on Embodied Cognition

Chen Chen,Zhang Junhui 한중경제문화학회 2022 한중경제문화연구 Vol.20 No.-

Smad4 mediates malignant behaviors of human ovarian carcinoma cell through the effect on expressions of E-cadherin, plasminogen activator inhibitor-1 and VEGF

( Chen Chen ),( Ming Zhong Sun ),( Shu Qing Liu ),( Dong Mei Yeh ),( Li Jun Yu ),( Yang Song ),( Lin Lin Gong ),( Li Hong Hao ),( Jun Hu ),( Shu Juan Shao ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.8

Smad4 is involved in cancer progression and metastasis. Using a pair of human syngeneic epithelial ovarian cancer cells with low (HO-8910) and high (HO-8910PM) metastatic abilities, we aimed to reveal the role of Smad4 in ovarian cancer metastasis in vitro. Smad4 was down-regulated in HO-8910PM cell line relative to HO-8910 by implicating Smad4 was probably a potential tumor suppressor gene for ovarian cancer. Re-expression of Smad4 decreased the migration ability and inhibited the invasion capacity of HO-8910PM, while promoted the cell adhesion capacity for HO-8910PM. The stable expression of Smad4 increased the expression of E-cadherin, reduced the expression of plasminogen activator inhibitor-1 (PAI-1) and slightly down-regulated the expression of VEGF. Smad4 suppresses human ovarian cancer cell metastasis potential through its effect on the expressions of PAI-1, E-cadherin and VEGF. Results from current work implicate Smad4 might suppress the invasion and metastasis of human ovarian tumor cells through a TGF-β/Smad-mediated pathway. [BMB reports 2010; 43(8): 554-560]

Fuzzy adaptive control particle swarm optimization based on T-S fuzzy model of maglev vehicle suspension system

Chen Chen,Junqi Xu,Guobin Lin,Yougang Sun,Dinggang Gao 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.1

At present, with the gradual promotion of Maglev vehicles, the stability of the suspension system has gradually become a hotspot. During the operation of Maglev vehicles, vibration may be caused by external disturbances such as track irregularity, non-directional wind load and load variation. When the vibration amplitude is within the controllable range of the current parameters, the restraint effect can be achieved and the stable convergence can be formed. However, when the vibration amplitude exceeds the current controllable range, the maglev vehicle may break the track or even lose stability. In order to solve the possible adverse effects of external disturbances on the stability of the system, a T-S fuzzy model considering both parameter uncertainties and external disturbances is constructed, and a relatively mature fuzzy adaptive control method is used for suspension control. However, considering the tracking performance of the system control parameters and the response speed of the parameter changes when the external disturbance changes, the particle swarm optimization (PSO) algorithm is used to optimize the system. The effectiveness of the optimized fuzzy adaptive control law in coordinating the closed-loop stability of the suspension system is proved in terms of response speed and convergence performance. Based on linear matrix inequality (LMI), the control response region satisfying the control performance after optimization is defined, and Lyapunov method is adopted to prove the stability of the optimized algorithm in controlling vehicle fluctuation operation. The simulation and experimental results show that the fuzzy adaptive control algorithm optimized by particle swarm optimization can further improve the speed of parameter optimization and the tracking performance of the system in the face of external disturbances and internal system parameter perturbations within a given range of control parameters. Compared with previous control strategies, the controller can greatly improve the response speed and the closed-loop information updating ability of the system in the face of disturbances, so that the system has stronger robustness and faster dynamic response.