Antiangiogenic-Like Properties of Fermented Extracts of Ayurvedic Medicinal Plants

Che´rif Rabhi,Guillaume Arcile,Le´on Cariel,Christine Lenoir,Je´rome Bignon,Joanna Wdzieczak-Bakala,Jamal Ouazzani 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.9

The three ayurvedic medicinal plants, Withania somnifera, Emblica officinalis, and Bacopa monnieri, were extracted by high-pressure static extraction using the Zippertex® technology. The extracts were mixed to reach quantifiable amounts of active compounds identified by high-pressure liquid chromatography-mass spectrometry (HPLC-MS) analysis. The mixture of extracts was incubated with resting cells of the fungus Beauveria bassiana ATCC 7159. The fermentation promoted the fluidization of the starting dense mixture, while HPLC monitoring evidenced the disappearance of glucogallin from E. officinalis extract and the concomitant increase in gallic acid content. Topical exposure of the chick embryo chorioallantoic membrane (CAM) to the nonfermented extract led to the extensive necrosis and destruction of the treated membrane. However, the fermented extract was shown to be free of any toxicity. Furthermore, compared with the untreated CAM, the fermented sample reduced CAM vascularization, suggesting its antiangiogenic potency. The innocuity of the fermented extract was demonstrated using the in vivo LD50 test, the morphological examination of internal organs of treated rats, as well as the evaluation of blood biomarkers of liver damage (aspartate aminotransferase and alanine aminotransferase). The fermented extract was developed as a nutraceutical antiangiogenic treatment of age-related macular degeneration and commercialized in an oral form named Ethnodyne-Visio™.

제주 우수 육상경기 선수들의 년중 훈련효과 분석 및 처방

김철원,임상용,김승곤,오만원,김성찬,이창준,남사웅,이세형,류재청 濟州大學敎 體育科學硏究所 1996 체육과학연구 Vol.2 No.-

The study was undertaken to analysis, evaluate, and prescribe t h e annual effect of training of periodically anaerobic and aerobic motor ability for Che-Ju representive team of elementary, middle and high school athletes. first. the performance can be obtained when it was prescribed proper training after analysis of physical fitness by each events. second, individually fitness. scientific analysis. prescription. proper management of athlete will be necessary to apply. In addition to physiology aspect, it is essential to consider sport biomechanics. sport psychology when we prescribe specific events and necessary approach in demension coach-athlete-researcher for individual.

제주 우수 수영 선수들의 년중 훈련효과 분석 및 처방

김철원,임상용,김승곤,오만원,김성찬,이창준,남사웅,이세형,류재청 濟州大學敎 體育科學硏究所 1996 체육과학연구 Vol.2 No.-

The study was undertaken to analysis, evaluate, and prescribe the annual effect of training of periodically anaerobic and erobic motor ability for Che-Ju representive team of elementary, middle and high school athletes, first the performance can be obtained when it was prescribed proper training after analysis of physical fitness by each events, second individually fitness scientific analysis. prescription. proper management of athlete will be necessary to apply.

의료소비자의 관계혜태 지각이 관계의 질 및 행동의도에 미치는 영향

허철선(Che이-Seon Heo),유광우(Kwang-Woo Yoo) 한국무역연구원 2014 무역연구 Vol.10 No.1

Medical institutions are important to develop and maintain enduring relationship with medical service conswners. So, this research aims to find out the effect of medical service conswners perception of relational benefits on relationship quality and behavioral intention. On the basis of the literature review, the research model is established. This research has been done through the survey utilizing medical service consumers in Gangwondo, and the effective 368 questionnaires are tested for the research model. The main findings are as follows. First, among 5 key dimensions of relational benefits, psychological, customization, economic, and information benefits except social benefits have significant effects on relationship satisfaction. Also, medical institutions type and medical service consumers type as moderating variables are included concurrently, and the significant differences are found in other 4 benefits except relationship between social benefits and relationship satisfaction. Second, relationship satisfaction relates positively to relationship commitment and behavioral intention. But the normative commitment, one of 3 key dimensions of relationship commitment, has no significant effects on recommendation to others. Third, the revisit intention has significant effect on recommendation to others. Finally, the findings reveal that the overall goodness fit index of the model is comparatively good and model set in this research is significant. At the end of the research, managerial implications, limitations and future research directions are suggested.

의료 화상을 위한 전처리 필터들의 특성에 관한 연구

강명아,최종주,정종필,정채영,정남채 조선대학교 기초과학연구소 1997 自然科學硏究 Vol.20 No.1

There has been no fixed rule of pre-processing course required to do image processing that it was barely possible to find the proper pre-processing course, only to process with experience till now. In this paper, lots of application method of pre-processing algorithm, only available by the non-typified and experiential method, shall quantify and the algorithm method of algorithm according to the rule shall calculate the frequency of the value of each pixel gray level and the histogrm of the image. And then average it to all pixel number and finally set a high-ranking or optional % standardized by the average value in teh standard value. The result of a experiment showed the Median filter is superior to the Mean filter in all image of the smoothing filter. The sobel filter extracted feature. The laplacian filter presented sectional feature which not showed in original image.

홍삼 추출물이 감마선에 조사된 생쥐의 혈액상에 미치는 변화

장재철,전철,김동윤,김동조 群山大學校自然科學硏究所 1990 自然科學硏究 Vol.5 No.-

홍삼 추출물이 감마선에 조사된 생쥐의 혈액상에 미치는 변화를 알아보기 위하여 생리적 식염수군을 대조구로 하여, 홍삼추출물 투여구와 홍삼추출물 투여후 5Gy 감마선 전신조사구 및 5Gy 감마선만을 전신조사한 구 등 4구로 분류하였다. 홍삼 추출물 투여에 의한 감마선에 조사된 생쥐의 적혈구 수, 백혈구 수 및 혈소판 수는 회복되어졌으면 반면, 생쥐의 무게나 혈색소량은 거의 유의성이 없었다. 홍삼 추출물 투여구와 생리적 식염수 투여구에 5Gy 방사선 조사구의 백혈구수는 현저히 회복되었다. 이러한 결과는 홍삼 추출물이 방사선에 조사된 생쥐의 혈액상에 방호적인 효과와 회복 효과가 있다고 생각되었다. This study was carried out obtain changes of red ginseng extract on the blood in the gamma ray post-irradiated mice. The experimental groups were control(saline), ginseng, ginseng and wholebody irradiated(??), and wholebody irradiated(??). Gamma ray post-irradiation recoverd by the red ginseng extract were observed in erythrocyte, leucocyte and thrombocyte, while the ginseng extract did not affect weight and hemoglobin. Leucocyte of ginseng and wholebody irradiated groups were significantry recoverd. This results thought that red ginseng extract group before radiation injury have the protective effect and the recovry effects on the mice blood.

Kim,Dong-jo

장재철,김동윤,박영순,전철,김동조 群山大學校 自然科學硏究所 1991 自然科學硏究 Vol.6 No.-

홍삼 추출물이 반치사량정도의 방사선(5Gy)에 조사된 생쥐조직 및 이들 조직에서의 효소활성에 미치는 영향을 관찰하기 위하여 ICR계 생쥐를 대상으로, 생리적 식염수군을 대조구군으로 하여, 홍삼 추출물 투여군과 방사선만을 조사한군 및 홍삼 추출물 투여후 방사선 조사군 등 4군으로 나누어 1일, 4일, 9일, 17일 및 30일 등 5회에 걸쳐 생체의 조직중 비장 및 고환에서의 중량 변화 및 GOT와 GPT의 활성을 측정하여 결과를 얻었다. 비장의 중량변화는 1일째 및 4일째 최소치를 보이다가 이후 증가추세를 보였으며, 고환의 중량 변화는 17일 이후 급격한 감소로 30일에도 증가하지 못했다. GOT의 활성도 변화는 비장에서는 초기에 현저히 증가했으며 이후 회복하는 경향이었다. 방사선 조사군들에서는 홍삼추출물 투여군이 방사선만을 조사한 군보다 빠르게 회복하는 경향이었으며 고환에서의 GOT활성은 매우 불안정하였다. GPT 활성도 변화는 비장, 고환에서 모두 초기에 증가하다가 점차 회복하는 경향이었으며 홍삼 추출물 투여후 방사선 조사군이 방사선만을 조사한군보다 빠르게 회복하는 경향이었다. 이러한 결과로 볼 때 홍삼 추출물은 방사선 조사에 의하여 기인된 비장 및 고환의 GOT와 GPT활성도 증가에 방호효과가 있다고 생각된다. This study was prepared to observe the change of tissues weight, glutamate oxaloacetate transminase(GOT) and glutamate pyruvate transminase(GPT) activities in the spleen and testis treated with red ginseng extract injection in the gamma ray irradiated mice. Groups were divided into control, red ginseng extract injection, irradiation after saline injection and irradiation after red ginseng extract injection. The spleen and testis weights were decreased at 17th day irradiation after saline injection and irradiation after red ginseng extract injection. The GOT activity showed earlier recovery at irradiation after red ginseng extract injection group than irradiation after saline injection group in the spleen. The GPT activity showed earlier recovery at irradiation after red ginseng extract injection group than irradiation after saline injection group in the spleen and testis. The above result suggest that red ginseng extract have the recovery effect on the change GOT and GPT activity after radiation injury in the spleen and testis of mice.

스쿠버 활동시 감압증 이론과 관련한 제요인의 원인과 예방법에 관한 고찰

류재청 濟州大學敎 體育科學硏究所 1997 체육과학연구 Vol.3 No.-

This review was undertaken to search the way for safe diving concerned with decompression sickness and types. Nitrogen saturation diving. Recompression equipments and theories of decompresiion sickness 1. The orgin og decompression theory Recent all theories of decompression sickness was originated from 「Caisson or Bends」 mean and based on Haldene thoery(1908). Theory shifted rom Haldene theory after that were theory of saturation diving. Haldane-Royal Navy table. U.S. Navy Diving Table(1965). Royal Navy Theory. Swiss theory, Canada theory, Dive Science and Technology Corporation(DSAT. 1987) 2. Women and decompression Woman were more suspectible to deccompression sickness than men because of first. having more body fat tissue. second more supectible decompression due to stream of body fluid during mensturation. third unbalanced circulation due to birth control pill. The symptoms generating in diving of pregnanent women consist of squeeze, air embolism. Decompression sickness but these did not any problem on fetus. 3. Equipments for prevention of decompression sickness 1) Dive tables Many decompression tables developed on the basis of on U.S. Navy Diving Table used Haldene theory were NAUI. PEDI. BASAC etc. and the content composed in common was to avoid decompression sicness in safety stop(3min stop at 15 feet). The skill to be practice on dive tables conist of resign of repetitive diving letter. Adjusted Maximum Dive Time(AMDT). min. surface interval time). Allowable max. depth in repetitive diving. RNT. SIT etc. 2) Gagues The most popular gauage using to avoid decompression sickness were capillary type depth gauge. diaphragm type depth gauge, oil-filled type depth gauge, analog digital type, analog digital submersible type. dive computer. Particularly It is recommanded to use dive computer display accurate absorption of Nitrogen, depth to avoid penalty of 「maximum-time at maximum-depth」 in multi-level diving. 3) Decompression prevention It is said to redecompression to treat serious symptom of decompression & air embolism. operated in recompression chambers. If you omitted safety stop. It is necessory to position of shock prevention, must be rest providing with pure 100% oxygen certifing symptom of decompression sickness, and be carried to hyperbaric facility. The height of flying after dive must not be superceded 8000 feet, at least before 24 hours. It is desirable to dive shorter and deeper than shallower and longer diving when scheduled of flight.

Heinrich Heine 詩論 : 特히 "Neue Gedichte"를 中心으로

轉在弘 釜山大學校 師範大學 1978 교사교육연구 Vol.5 No.1

Welch ein Lied! "Eine gewaltige Lust ergreift mich! Wa˝hrend ich sitze, und schreibe, erklingt Musik unter meinem Fenster, und an dem elegischen Grimm der lang-gezogenen Melodie, erkenne ich jene Marseiller Hymne, womit der scho˝ne Barbaroux und seine Gefa˝hrten die. Stadt Paris begru˝Bten, jener Kuhreigen der Freiheit, bei dessen To˝nen die Schweizer in den Tuilerien das Heimweh bekamen, jener triumphierende Todesgesang der Gironde, das alte su˝Be Wiegenlied-Es durehschauert mich mit Feuer und Freude, und entzU˝ndet in mir die glU˝henden Sterne der Begeisterung und die Raketen des Spottes. Ja, diese sollen nicht fehlen, bei dem groBen Feuerwerk der Zeit. Klingende Frammenstro˝me des Gesanges sollen sich ergieBen von der Ho˝he der Freiheitslust, in ku˝hnen Kaskaden, wie sich der Ganges herabstU˝rzt vom Himalaya! Und du, holde Satyra, Tochter der gerechten Themis und des bocksfu˝Bigen Pan, leih mir deine Hu˝lfe, Du bist ja mu˝tterlicher Seite dem Titanengeschlechte entsprossen, und hassest gleich mir die Feinde deiner Sippschaft, die schwa˝chlichen Usurpatoren des Olymps. Leih mir das Schwert deiner Mutter. damit ich sie richte, die verhaBte Brut, und gib mir die Picke-lflo˝te deines Vaters, damit ich sie zu Tode pfeife-">>schrieb Heinrich Heine die Spa˝tere Nachschrift der'〉Reisebilder'<<schon im November 1830. Nachdem Heine den Reisestoff auf die beschriebene Weise aufgearbeitet hatte, war er in politischer Hinsicht fu˝r ihn abgeschlossen. Heines Bedu˝rfnis nach Variation lieB eine einfache Wiederholung nicht zu. So arbeitete er mit neuem Stil das>'Neuer Fru˝hling'<1828/31von der Neuen Gedichte in Hamburg auf. Die Chance fu˝r eine. Realisierung ist hier gro˝Ber. In dieser Phase ist ihm die politisch-gesellschaftliche Aufgabe wichtiger als die dichterische. Denn Heine mo˝chte als politischer Befreier in die Geschichte eingehen. Auch an seinen Werk Neuer Fru˝hling<' wendet sich das Technische von Metrik, Sprache und Form.

해조류 에탄올 농도별 추출물의 항돌연변이원성 및 항균성

박제석,오명철,오창경,현재석,김봉오,김수현 濟州大學校工科大學産業技術硏究所 1996 尖端技術硏究所論文集 Vol.7 No.2

톳. 미역 및 다시마의 에탄올 농도별 추출물이 MMS. ICR191 및 sodium azide에 의해 유도되는 돌연변이원성의 억제효과 및 E. coli, B. subtilis 및 S. serevisiae에 대한 항균효과를 검정하였다. 톳의 90% 에탄올 추출물은 MMS와 ICR191에 대한 항돌연변이 원성이 각각 70 및 95% 이상으로 매우 높았으나, sodium azide에 대한 항돌연변이원성은 없었다. 미역의 에탄올 농도별 추출물은 30∼90% 에탄올 추출물에서 50% 이상의 항돌연변이 효과를 보았으나 MMS와 sodium azide에 대한 항돌연변이 효과는 없었다. 다시마의 30∼90% 에탄올 추출물의 ICR191에 대한 항돌연변이 효과는 70% 이상이었으나, MMS와 sodium azide에 대한 효과는 없었다. 에탄올 농도별 톳의 추출물들에 대한 항균성 검색 결과 70과 90% 추출물들이 E. coli와 B. subtilis에 대하여 뚜렷한 항균효과는 미역 추출물 보다 다시마의 추출물이 더욱 요과적이었다. 미역 추출물은 50% 추출물만이 S. cerevisiae에 대한 성장저지효과를 나타낸 반면, 다시마 추출물은 90% 에탄올 추출물에서는 시험된 모든 공시균주에 대하여, 그리고 70% 에탄올 추출물에서는 E. coli와 B. sutilis에 대하여 높은 성장저지효과를 나타내었다. Desmutagenic against MMS-, ICR191- and sodium azide-induced mutations, and antimicrobial effects against Escherichia coli, Bacillus subtilis and Saccharomyces cerevisiae by the extracts from Hizikia fusiforme. Laminaria japonica and Undaria pinnatifida with ethanol of a different concentrations were investigated. Desmutagenic effects against MMS and ICR191 of 90% ethanol extracts from Hizikia fusiforme were up 70 and 95%. respectively. no against sodium azide. Desmutagenic effects against ICR191 of 30∼90% ethanol extracts from Undaria pinnatifida and Laminaria japonica were up to 50 and 70%. respectively. but no against sodium azide and MMS. Antimicrobial effect of 70 and 90% ethanol extracts from Hizikia fusiforme had potent antimicrobial activities against E. coli and B. subtilis. Ethanol extracts from Laminaria japonica showed more effective antimicrobial effects than those from Undaria pinnatifida. Only 50% ethanol extract from Undaria pinnatifida showed growth inhibition effect against S. cerevisiae. Seventy percent ethanol extract from Laminaria japonica showed growth inhibition effect against all strains tested. while 90% ethanol extract showed against E. coli and B. subtilis.