Application of Response Surface Methodology for Optimization of Lactic Acid Production Using Date Juice

( Chauhan ),( Kishor ),( Ujjval Trivedi ),( K. C. Patel ) 한국미생물생명공학회 2006 Journal of microbiology and biotechnology Vol.16 No.9

Ginkgo biloba Administered Singly and Combined With Antioxidants in Tinnitus Patients

Chauhan Bhushan,Arya Shantanu,Chauhan Komal 대한청각학회 2023 Journal of Audiology & Otology Vol.27 No.1

Background and Objectives: Tinnitus, or ear ringing, involves impulsive and spontaneous activity in the auditory neurons. Its prevalence is high in the elderly, but 10%–15% of adults suffer from tinnitus, affecting their quality of life. Therefore, this study aimed to evaluate the efficacy of <i>Ginkgo biloba</i> administered singly and in combination with antioxidants in tinnitus patients.Subjects and Methods: Patients were randomly allocated to Placebo (T0, n=22), Treatment 1 (T1, n=24), and Treatment 2 (T2, n=23) groups. The patients were educated on the study’s methodology and were instructed to visit at 0, 4, 8, 12, and 14 weeks. The placebo group received starch capsule supplements. Conversely, the treatment groups received <i>Ginkgo biloba</i> (60 mg twice a day) singly and in combination with antioxidants. We enrolled 69 patients aged 40–70 years (41 men, 28 women). The Tinnitus Handicap Index (THI), Visual Analogue Score (VAS), and Short Form 36 (SF-36) Health Scores were determined pre- and post-treatment at each visit.Results: Supplementation of <i>Ginkgo biloba</i>, along with antioxidants, provided marked improvement (<i>p</i><0.05) in post-treatment THI and VAS scores in the T2 group compared to those in the T1 and T0 groups. The greatest (<i>p</i><0.05) percent difference was observed in the pre- and post-treatment THI (-36%) and VAS scores (-22.6%) of T2 patients. Likewise, the SF-36 scores improved significantly (<i>p</i><0.05) in the T2 group in varied parameters.Conclusions: <i>Ginkgo biloba</i>, along with antioxidants, can be a promising therapy for tinnitus patients, providing marked improvement in THI, VAS, and SF-36 scores.

Role of Devnagari and Persian Script in Hindi-Urdu Controversy: A Historical Perspective

( Chauhan Karan Singh ) 한국인도학회 2011 印度硏究 Vol.16 No.1

Controversy regarding the language of the so-called ``Hindustani`` area has been an important issue throughout British period which engulfed the Indian freedom movement. Hindi or ``Hindui`` was the original form adopted and mentioned as such by the earliest of writers of Hindi. Later, word Urdu came into use to denote the same language called Hindi or Hindui, but with a Persian tilt. The common people continued to regard both forms as one language. Due to Moghal rule, Hindi and later Urdu written in Persian script became the official language of governance. When Britishers came to India they found a very interesting phenomena with regard to language and scripts pravelent in northern Hindi belt. Although the spoken language of the people was one, but it was written in a script, imposed from outside. They tried to confirm the real position by doing various language and script surveys and came to the conclusion that it would be better for communication and understanding if beside Persian script, ``Nagari`` script is introduced in Government offices and courts etc. This angered the Muslim community who used to be in an advantageous position with Persian script. Although, language question was never a controversial issue among the common people of this area but due to the two different scripts, it got associated with communal feelings. The emergence of Sanskritized Hindi and Persianized Urdu was the direct outcome of this communal divide. Britishers tried to not only placate this but took full advantage of the issue for dividing people on communal lines. To create communal unity within freedom struggle, Gandhi Ji tried to bring ``Hindustani`` into focus which represent the mix of both spoken Hindi and Urdu. He advocated one common language for both the communities which can be written either in Nagari or Persian script. It attracted lot of criticism from both sides. In such a charged atmosphere the question of National language or a National script became more and more controversial. Progressive cultural movement, being the most important literary movement in all Indian Languages, where both Hindi and Urdu writers worked together, tried to understand and solve the language and script problem. On the basis of its analysis and conclusions it mendated that Hindi written in Devnagari script should be the national language of the country and Urdu written in Persian should be regarded as close associate of it. Later, when Indian constitution was framed and implemented, in article 343 regarding the Official language of the Union it was written that ``the official language of the Union shall be Hindi in Devnagari script.``

STRONG CONVERGENCE THEOREMS FOR A QUASI CONTRACTIVE TYPE MAPPING EMPLOYING A NEW ITERATIVE SCHEME WITH AN APPLICATION

Chauhan, Surjeet Singh,Utreja, Kiran,Imdad, Mohammad,Ahmadullah, Md The Honam Mathematical Society 2017 호남수학학술지 Vol.39 No.1

In this paper, we introduce a new scheme namely: CUIA-iterative scheme and utilize the same to prove a strong convergence theorem for quasi contractive mappings in Banach spaces. We also establish the equivalence of our new iterative scheme with various iterative schemes namely: Picard, Mann, Ishikawa, Agarwal et al., Noor, SP, CR etc for quasi contractive mappings besides carrying out a comparative study of rate of convergences of involve iterative schemes. The present new iterative scheme converges faster than above mentioned iterative schemes whose detailed comparison carried out with the help of different tables and graphs prepared with the help of MATLAB.

Competitive interactions between weedy rice and cultivated rice as a function of added nitrogen and the level of competition

Chauhan, Bhagirath S.,Johnson, David E. The Korean Society of Weed Science and The Turfgra 2011 Weed Biology and Management Vol.11 No.4

The competitive outcomes between weedy rice from Malaysia (MWR), the Philippines (PWR), and Vietnam (VWR) and cultivated rice (IR64) grown in pots were evaluated in a replacement series experiment with added N (0, 50, 100, and 150 kg N $ha^{-1}$) and competition with IR64 plants (no competition, eight weedy rice plants : 0 IR64 plants; low competition, six weedy rice plants : two IR64 plants; and high competition, two weedy rice plants : six IR64 plants). The growth observations were taken at 10 weeks after sowing. When grown in a monoculture (no competition with IR64 plants), the PWR plants had a lower shoot biomass across N rates than did the MWR andVWR plants.The leaf area and shoot biomass of weedy rice across populations significantly increased with an increase in the N application rate. Each weedy rice population and the IR64 population showed linear responses of the leaf area and shoot biomass to the N rate at all levels of competition. The weedy rice and IR64 plants, when grown without competition, had a similar rate of response in the shoot biomass to the N rate. However, when grown in competition, the response to the added N varied among the weedy rice populations. The MWR plants under competition produced a similar amount of shoot biomass to the IR64 plants per unit addition of N. In contrast, the PWR and VWR populations under competition produced a greater amount of shoot biomass with each additional unit of N, compared to the IR64 population. The results illustrate that N fertilizer management might affect the outcome of weedy rice competition. This information could be incorporated into weedy rice management strategies.

Role of Devnagari and Persian Script in Hindi-Urdu Controversy

Chauhan Karan Singh 한국인도학회 2011 印度硏究 Vol.16 No.2

Controversy regarding the language of the so-called ‘Hindustani’ area has been an important issue throughout British period which engulfed the Indian freedom movement. Hindi or ‘Hindui’ was the original form adopted and mentioned as such by the earliest of writers of Hindi. Later, word Urdu came into use to denote the same language called Hindi or Hindui, but with a Persian tilt. The common people continued to regard both forms as one language. Due to Moghal rule, Hindi and later Urdu written in Persian script became the official language of governance. When Britishers came to India they found a very interesting phenomena with regard to language and scripts pravelent in northern Hindi belt. Although the spoken language of the people was one, but it was written in a script, imposed from outside. They tried to confirm the real position by doing various language and script surveys and came to the conclusion that it would be better for communication and understanding if beside Persian script, ‘Nagari’ script is introduced in Government offices and courts etc. This angered the Muslim community who used to be in an advantageous position with Persian script. Although, language question was never a controversial issue among the common people of this area but due to the two different scripts, it got associated with communal feelings. The emergence of Sanskritized Hindi and Persianized Urdu was the direct outcome of this communal divide. Britishers tried to not only placate this but took full advantage of the issue for dividing people on communal lines. To create communal unity within freedom struggle, Gandhi Ji tried to bring ‘Hindustani’ into focus which represent the mix of both spoken Hindi and Urdu. He advocated one common language for both the communities which can be written either in Nagari or Persian script. It attracted lot of criticism from both sides. In such a charged atmosphere the question of National language or a National script became more and more controversial. Progressive cultural movement, being the most important literary movement in all Indian Languages, where both Hindi and Urdu writers worked together, tried to understand and solve the language and script problem. On the basis of its analysis and conclusions it mendated that Hindi written in Devnagari script should be the national language of the country and Urdu written in Persian should be regarded as close associate of it. Later, when Indian constitution was framed and implemented, in article 343 regarding the Official language of the Union it was written that ‘the official language of the Union shall be Hindi in Devnagari script.’

Development of In Vitro Produced Buffalo (Bubalus bubalis) Embryos in Relation to Time

Chauhan, M.S.,Singla, S.K.,Palta, P.,Manik, R.S.,Tomer, O.S. Asian Australasian Association of Animal Productio 1998 Animal Bioscience Vol.11 No.4

The objective of the present study was to examine the developmental rates, and the stage of development in relation to time since fertilization, of in vitro produced buffalo embryos. Buffalo cumulus-oocyte complexes obtained from slaughterhouse ovaries were matured and fertilized in vitro. The fertilized oocytes (n = 248) were then co-cultured with buffalo oviductal epithelial cells and evaluated for the developmental stages on Days 2, 4, 6, 7, 8, 9 and 10 post-insemination. The peak of 4-cell stage embryos was observed on Day 2 (63.7 %), whereas Day 4 was marked by peaks of 6-8-cell stage embryos (20.9%) and 16-cell stage embryos to early morulae (50%). On Days 6, 7, 8, 9, and 10 post-insemination, 49.5, 48.3, 38.3, 33.8 and 33.4% embryos were found to be at morula/compact morula stages, 8.8, 12.5, 25.4, 6.0 and 1.2% at early blastocyst/blastocyst stages, 0, 6.8, 7.2, 15.3 and 2.0% at expanded blastocyst stage and 0, 1.6, 4.8, 19.3 and 38.5% hatching/hatched blastocyst stages, respectively. The peaks of early blastocyst/blastocyst, expanded blastocyst and hatching/hatched blastocyst stages were observed on Days 8, 9 and 10, respectively. The percentages of oocytes which initially became arrested and subsequently degenerated were 3.6, 4.8, 10.4, 14.5, 21.3 and 24.5% on Days 4, 6, 7, 8, 9 and 10 post-insemination, respectively.

Detection and purification of backbone-cyclized proteins using a bacterially expressed anti-myc-tag single chain antibody

Chauhan, Sushma,Hou, Chen Yuan,Jung, Sang Taek,Kang, Taek Jin Academic Press 2017 Analytical biochemistry Vol.532 No.-

<P><B>Abstract</B></P> <P>A myc-tag and of which recognition by an antibody 9E10 has long been used for the detection and purification of recombinant proteins. We have previously expanded the application of the tag to the specific detection and purification of backbone-cyclized proteins. Here we sought a more practical way to using the 9E10 antibody by expressing its single chain antibody (scAb) form in <I>Escherichia coli</I>. The combined use of a strong T7 promoter and auto-induction strategy rather than early to mid-log induction of a Lac promoter resulted in the soluble over-expression of 9E10 scAb. However, the co-expression of a chaperone, Skp, was absolutely necessary for the activity even when the protein was expressed in a soluble manner. We could purify about 4 mg of 9E10 scAb from 1 l of culture, and the resulting scAb could be used to detect and purify the backbone-cyclized protein as the parental full-length 9E10. Moreover, the immunoaffinity resin prepared using 9E10 scAb could be regenerated several times after the elution of bound proteins using an acid, which added more value to the ready preparation of the active antibody in bacteria.</P>

Thymol elicits HCT-116 colorectal carcinoma cell death through induction of oxidative stress

Chauhan, Anil,Bahuguna, Ashutosh,Paul, Souren,Kang, Sun Bentham Science 2017 ANTICANCER AGENTS IN MEDICINAL CHEMISTRY Vol.17 No.999

Use of Terminal Restriction Length Polymorphism (T-RFLP) Analysis to Evaluate Uncultivable Microbial Community Structure of Soil

Chauhan, Puneet Singh,Shagol, Charlotte C.,Yim, Woo-Jong,Tipayno, Sherlyn C.,Kim, Chang-Gi,Sa, Tong-Min Korean Society of Soil Science and Fertilizer 2011 한국토양비료학회지 Vol.44 No.1

Various environmental ecosystems are valuable sources for microbial ecology studies, and their analyses using recently developed molecular ecological approaches have drawn significant attention within the scientific community. Changes in the microbial community structures due to various anthropogenic activities can be evaluated by various culture-independent methods e.g. ARISA, DGGE, SSCP, T-RFLP, clone library, pyrosequencing, etc. Direct amplification of total community DNA and amplification of most conserved region (16S rRNA) are common initial steps, followed by either fingerprinting or sequencing analysis. Fingerprinting methods are relatively quicker than sequencing analysis in evaluating the changes in the microbial community. Being an efficient, sensitive and time- and cost effective method, T-RFLP is regularly used by many researchers to access the microbial diversity. Among various fingerprinting methods T-RFLP became an important tool in studying the microbial community structure because of its sensitivity and reproducibility. In this present review, we will discuss the important developments in T-RFLP methodology to distinguish the total microbial diversity and community composition in the various ecosystems.