Application of Response Surface Methodology for Optimization of Lactic Acid Production Using Date Juice

( Chauhan ),( Kishor ),( Ujjval Trivedi ),( K. C. Patel ) 한국미생물생명공학회 2006 Journal of Microbiology and Biotechnology Vol.16 No.9

Ginkgo biloba Administered Singly and Combined With Antioxidants in Tinnitus Patients

Chauhan Bhushan,Arya Shantanu,Chauhan Komal 대한청각학회 2023 Journal of Audiology & Otology Vol.27 No.1

Background and Objectives: Tinnitus, or ear ringing, involves impulsive and spontaneous activity in the auditory neurons. Its prevalence is high in the elderly, but 10%–15% of adults suffer from tinnitus, affecting their quality of life. Therefore, this study aimed to evaluate the efficacy of <i>Ginkgo biloba</i> administered singly and in combination with antioxidants in tinnitus patients.Subjects and Methods: Patients were randomly allocated to Placebo (T0, n=22), Treatment 1 (T1, n=24), and Treatment 2 (T2, n=23) groups. The patients were educated on the study’s methodology and were instructed to visit at 0, 4, 8, 12, and 14 weeks. The placebo group received starch capsule supplements. Conversely, the treatment groups received <i>Ginkgo biloba</i> (60 mg twice a day) singly and in combination with antioxidants. We enrolled 69 patients aged 40–70 years (41 men, 28 women). The Tinnitus Handicap Index (THI), Visual Analogue Score (VAS), and Short Form 36 (SF-36) Health Scores were determined pre- and post-treatment at each visit.Results: Supplementation of <i>Ginkgo biloba</i>, along with antioxidants, provided marked improvement (<i>p</i><0.05) in post-treatment THI and VAS scores in the T2 group compared to those in the T1 and T0 groups. The greatest (<i>p</i><0.05) percent difference was observed in the pre- and post-treatment THI (-36%) and VAS scores (-22.6%) of T2 patients. Likewise, the SF-36 scores improved significantly (<i>p</i><0.05) in the T2 group in varied parameters.Conclusions: <i>Ginkgo biloba</i>, along with antioxidants, can be a promising therapy for tinnitus patients, providing marked improvement in THI, VAS, and SF-36 scores.

PTK7⁺ Mononuclear Cells Express VEGFR2 and Contribute to Vascular Stabilization by Upregulating Angiopoietin-1

Chauhan, Sunil K.,Lee, Hyung Keun,Lee, Hyun Soo,Park, Eun Young,Jeong, Eunae,Dana, Reza American Heart Association, Inc. 2015 Arteriosclerosis, thrombosis, and vascular biology Vol.35 No.7

<P><B>Objective—</B></P><P>In angiogenesis, circulating mononuclear cells are recruited to vascular lesions; however, the underlying mechanisms are poorly understood.</P><P><B>Approach and Results—</B></P><P>Here, we characterize the functional role of protein tyrosine kinase 7 (PTK7)–expressing CD11b<SUP>+</SUP> mononuclear cells in vitro and in vivo using a mouse model of angiogenesis. Although the frequencies of PTK7<SUP>+</SUP>CD11b<SUP>+</SUP> cells in the bone marrow remained similar after vascular endothelial growth factor-A–induced neovascularization, we observed an 11-fold increase in the cornea. Importantly, vascular endothelial growth factor-A–induced chemotaxis of PTK7<SUP>+</SUP> cells was mediated by vascular endothelial growth factor receptor 2. In a coculture with endothelial cells, PTK7<SUP>+</SUP>CD11b<SUP>+</SUP> cells stabilized the vascular network for 2 weeks by expressing high levels of angiopoietin-1. The enhanced vascular stability was abolished by knockdown of angiopoietin-1 in PTK7<SUP>+</SUP>CD11b<SUP>+</SUP> cells and could be restored by angiopoietin-1 treatment.</P><P><B>Conclusions—</B></P><P>We conclude that PTK7 expression in perivascular mononuclear cells induces vascular endothelial growth factor receptor 2 and angiopoietin-1 expression and thus contributes to vascular stabilization in angiogenesis.</P>

Use of Terminal Restriction Length Polymorphism (T-RFLP) Analysis to Evaluate Uncultivable Microbial Community Structure of Soil

Chauhan, Puneet Singh,Shagol, Charlotte C.,Yim, Woo-Jong,Tipayno, Sherlyn C.,Kim, Chang-Gi,Sa, Tong-Min Korean Society of Soil Science and Fertilizer 2011 한국토양비료학회지 Vol.44 No.1

Various environmental ecosystems are valuable sources for microbial ecology studies, and their analyses using recently developed molecular ecological approaches have drawn significant attention within the scientific community. Changes in the microbial community structures due to various anthropogenic activities can be evaluated by various culture-independent methods e.g. ARISA, DGGE, SSCP, T-RFLP, clone library, pyrosequencing, etc. Direct amplification of total community DNA and amplification of most conserved region (16S rRNA) are common initial steps, followed by either fingerprinting or sequencing analysis. Fingerprinting methods are relatively quicker than sequencing analysis in evaluating the changes in the microbial community. Being an efficient, sensitive and time- and cost effective method, T-RFLP is regularly used by many researchers to access the microbial diversity. Among various fingerprinting methods T-RFLP became an important tool in studying the microbial community structure because of its sensitivity and reproducibility. In this present review, we will discuss the important developments in T-RFLP methodology to distinguish the total microbial diversity and community composition in the various ecosystems.

Ancient Indian Approach to Environment

Chauhan. Karan S. 한국외국어대학교 외국학종합연구센터 남아시아연구소 2000 남아시아연구 Vol.5 No.-

Development of In Vitro Produced Buffalo (Bubalus bubalis) Embryos in Relation to Time

Chauhan, M.S.,Singla, S.K.,Palta, P.,Manik, R.S.,Tomer, O.S. Asian Australasian Association of Animal Productio 1998 Animal Bioscience Vol.11 No.4

The objective of the present study was to examine the developmental rates, and the stage of development in relation to time since fertilization, of in vitro produced buffalo embryos. Buffalo cumulus-oocyte complexes obtained from slaughterhouse ovaries were matured and fertilized in vitro. The fertilized oocytes (n = 248) were then co-cultured with buffalo oviductal epithelial cells and evaluated for the developmental stages on Days 2, 4, 6, 7, 8, 9 and 10 post-insemination. The peak of 4-cell stage embryos was observed on Day 2 (63.7 %), whereas Day 4 was marked by peaks of 6-8-cell stage embryos (20.9%) and 16-cell stage embryos to early morulae (50%). On Days 6, 7, 8, 9, and 10 post-insemination, 49.5, 48.3, 38.3, 33.8 and 33.4% embryos were found to be at morula/compact morula stages, 8.8, 12.5, 25.4, 6.0 and 1.2% at early blastocyst/blastocyst stages, 0, 6.8, 7.2, 15.3 and 2.0% at expanded blastocyst stage and 0, 1.6, 4.8, 19.3 and 38.5% hatching/hatched blastocyst stages, respectively. The peaks of early blastocyst/blastocyst, expanded blastocyst and hatching/hatched blastocyst stages were observed on Days 8, 9 and 10, respectively. The percentages of oocytes which initially became arrested and subsequently degenerated were 3.6, 4.8, 10.4, 14.5, 21.3 and 24.5% on Days 4, 6, 7, 8, 9 and 10 post-insemination, respectively.

RIP1-dependent reactive oxygen species production executes artesunate-induced cell death in renal carcinoma Caki cells

Chauhan, A. K.,Min, K. j.,Kwon, T. K. Springer Science + Business Media 2017 MOLECULAR AND CELLULAR BIOCHEMISTRY - Vol.435 No.1

STRONG CONVERGENCE THEOREMS FOR A QUASI CONTRACTIVE TYPE MAPPING EMPLOYING A NEW ITERATIVE SCHEME WITH AN APPLICATION

Chauhan, Surjeet Singh,Utreja, Kiran,Imdad, Mohammad,Ahmadullah, Md The Honam Mathematical Society 2017 호남수학학술지 Vol.39 No.1

In this paper, we introduce a new scheme namely: CUIA-iterative scheme and utilize the same to prove a strong convergence theorem for quasi contractive mappings in Banach spaces. We also establish the equivalence of our new iterative scheme with various iterative schemes namely: Picard, Mann, Ishikawa, Agarwal et al., Noor, SP, CR etc for quasi contractive mappings besides carrying out a comparative study of rate of convergences of involve iterative schemes. The present new iterative scheme converges faster than above mentioned iterative schemes whose detailed comparison carried out with the help of different tables and graphs prepared with the help of MATLAB.

Indian Science and Technology at the Time of British Occupation

Chauhan, Karan S. 한국외국어대학교 남아시아연구소 2001 남아시아연구 Vol.6 No.-

Competitive interactions between weedy rice and cultivated rice as a function of added nitrogen and the level of competition

Chauhan, Bhagirath S.,Johnson, David E. The Korean Society of Weed Science and The Turfgra 2011 Weed Biology and Management Vol.11 No.4

The competitive outcomes between weedy rice from Malaysia (MWR), the Philippines (PWR), and Vietnam (VWR) and cultivated rice (IR64) grown in pots were evaluated in a replacement series experiment with added N (0, 50, 100, and 150 kg N $ha^{-1}$) and competition with IR64 plants (no competition, eight weedy rice plants : 0 IR64 plants; low competition, six weedy rice plants : two IR64 plants; and high competition, two weedy rice plants : six IR64 plants). The growth observations were taken at 10 weeks after sowing. When grown in a monoculture (no competition with IR64 plants), the PWR plants had a lower shoot biomass across N rates than did the MWR andVWR plants.The leaf area and shoot biomass of weedy rice across populations significantly increased with an increase in the N application rate. Each weedy rice population and the IR64 population showed linear responses of the leaf area and shoot biomass to the N rate at all levels of competition. The weedy rice and IR64 plants, when grown without competition, had a similar rate of response in the shoot biomass to the N rate. However, when grown in competition, the response to the added N varied among the weedy rice populations. The MWR plants under competition produced a similar amount of shoot biomass to the IR64 plants per unit addition of N. In contrast, the PWR and VWR populations under competition produced a greater amount of shoot biomass with each additional unit of N, compared to the IR64 population. The results illustrate that N fertilizer management might affect the outcome of weedy rice competition. This information could be incorporated into weedy rice management strategies.