沈菜類 熟成에 따르는 Microflora, 糖, pH 및 Vitamin B_12의 變化와 Pseudomonas sp.의 Vitamin B_12 生産性에 관하여

黃圭贊,朴啓仁 慶熙大學校 1986 論文集 Vol.15 No.-

This study was to investigate the changes of microflora, sugar, pH, and vitamin B_(12) during the aging of fermented vegetables such as Tongkimchi and Dongchimi and the productivity of vitamin B_(12) by Pseudomonas denitrificans D-2-1 selected as vitamin B_(12) producing bacteria from Dongchimi. The main bacteria responsible for Tongkimchi fermertation were Lactobacillus plantarum, Leuconostoc mesenteroides and Streptococcus faecalis, but the main bacteria for Dongchimi fermentation were Leuconostoc mesenteroides and the genus Pseudomonas. In TongKimchi and Dongchimi sugar continuously decreased from ca. 3% and pH reduced from 5.8 to 4.0 and less, but increased as the over-ripening progresses. Vitamin B_(12) content of TonKimchi was higher than those of Dongchimi at the early stage, hut the opposite result at a later period. Vitamin B_(12) content of TongKimchi and Dongchimi reached its maximum at the middle period. Pseudomonas denitrificans D-2-1 cultured for 15 days on the media of radish juice produced vitamin B_(12) up to 274 ng/㎖, and the strain cultured on the media of radish juice containing 1.5% NaCl produced vitamin B_(12) of 261 ng/㎖. The strain cultured on the media of radish juice containing 3% NaCl and 0.1% L-glutamic acid as a nitrogen source produced vitamin B_(12) of 272 ng/㎖. In contrast to the amount of vitamin B_(12)(245 ng/㎖) obtained from the media in which L-glutamic acid was not added, the media containing L-glutamic acid increased the productivity of vitamin B_(12) up to 11%.

脫脂乳에서 Sodium Formate에 依한 Lactobacillus bulgaricus ATCC 11842 生育促進에 關하여

李讚,朴啓仁 慶熙大學校 1984 論文集 Vol.13 No.-

The growth stimulation of Lactobacillus bulgaricus ATCC 11842 by sodium formate, and the mixed culture of Lactobacillus bulgaricus and Streptococcus thermophilus was examined at 45℃ for 10-48 hrs. respectively. The periodical change of lactic acid production and viable count were analyzed. The results obtained are as follows: 1) When the mixed culture of L. bulgaricus and Str. thermophilus in 11% skim milk media was incubated by stationary culture, the lactic acid production was stimulated after 36 hrs. but was not stimulated in case of shaking culture at 140 rpm. 2) The lactic acid production of L. bulgaricus ATCC 11842 was more increased in 11% skim milk media sterilized at 121℃ for 15 minutes than in 11% skin media tyndallized for 2 days. 3) The lactic acid production and viable count of L. bulgaricus ATCC 11842 in 11% skim milk media added 65ppm sodium formate were increased considerably. 4) The maximum lactic acid content of L. bulgaricus ATCC 11842 in 11% skim milk added sodium formate was 0.55% at 100-110 ppm sodium formate. And the lactic acid production was completely inhibited at 200 ppm sodium formate.

沈菜類 熟成細菌의 分離 同定

黃圭贊,朴啓仁 慶熙大學校 1985 論文集 Vol.14 No.-

This study was to investigate the identification of bacteria take part in the aging of fermented vegetable such as Tongkimchi and Dongchimi, the fermented vegetables which are indigenous to Korea were prepared in laboratory scale according to the same method used by private house in Seoul. The analogy of aerobic and anaerobic bacterial flora isolated from Kimchi was found, and their bacterial flora were identified by investigating the cultural, morphological and physiological characteristics. In results obtained are as follows; 1. During the aging of Tongkimchi. bacterial flora found in the early stage were Leuconostoc mesenteriodes, Lactobacillus plantarum, Streptococcus faecails and Pediococcus pentosaceus. In the middle period, however, Lactobacillus plantarum and streptococcus paecalis were predominant, and Lactobacillus brevis and Streptococcus faecalis subsp. liquefaciens were also fund. 2. During the aging of Dongchimi, aeroges found in the early stage were the genus Pseudomonas, and anaerobes such as Leuconostoc mesenteroides, Lactobacillus breyis, Lactobacillus plantarum and Pediococcus pentosaceus were also found. In the middle period, however, Leuconostoc mesenteroides and Pseudomonas denitriflcans were more predominant.

沈菜類 熟成細菌의 分離 同定

黃圭贊,朴啓仁 경희대학교 부설 식량자원개발연구소 1985 硏究論文集 Vol.6 No.-

This study was to investigate the identification of bacteria take part in the aging of fermented vegetable such as Tongkimchi and Dongchimi; the fermented vegetables which are indigenous to Korea were prepared in laboratory scale according to the same method used by private house in Seoul. The analogy of aerobic and anaerobic bacterial flora isolated from Kimchi was found, and their bacterial flora were identified by investigating the cultural, morphological and physiological characteristics. In results obtained are as follows; 1. During the aging of Tongkimchi, bacterial flora found in the early stage were Leuconostoc mesenteriodes, Lactobacillus plantarum. Streptococcus faecalis and Pediococcus pentosaceus. In the middle period, however, Lactobacillus plantarum and Streptococcus faecalis were predominant, and Lactobacillus brevis and Streptococcus faecalis subsp. liquefaciens were also fund. 2. During the aging of Dongchimi, aerobes found in the early stage were the genus Pseudomonas, and anaerobes such as Leuconostoc mesenteroides, Lactobacillus breyis, Lactobacillus plantarum and Pediococcus pentosaceus were also found. In the middle period, however, Leuconostoc mesenteroides and Pseudomonas denitrificans were more predominant.

脫脂乳에서 Sodium Formate에 依한 Lactobacillus bulgaricus ATCC 11842 生育促進에 關하여

李讚,朴啓仁 경희대학교 부설 식량자원개발연구소 1984 硏究論文集 Vol.5 No.-

The growth stimulation of Lactobacillus bulgaricus ATCC 11842 by sodium formate, and the mixed culture of Lactobacillus bulgaricus and Streptococcus thermophilus was examined at 45℃ for 10-48 hrs. respectively. The periodical change of lactic acid production and viable count were analyzed. The results obtained are as follows: 1) When the mixed culture of L. bulgaricus and Str. thermophilus in 11% skim milk media was incubated by stationary culture, the lactic acid production was stimulated after 36 hrs. but was not stimulated in case of shaking culture at 140 rpm. 2) The lactic acid production of L. bulgaricus ATCC 11842 was more increased in 11% skim milk media sterilized at 121℃ for 15 minutes than in 11% skim media tyndallized for 2 days. 3) The lactic acid production and viable count of L. bulgaricus ATCC 11842 in 11% skim milk media added 65 ppm sodium formate were increased considerably. 4) The maximum lactic acid content of L. bulgaricus ATCC 11842 in 11% skim milk media added sodium formate was 0.55% at 100-110 ppm sodium formate. And the lactic acid production was completely inhibited at 200 ppm sodium formate.

沈菜類 熟成에 따르는 Microflora, 糖, pH 및 Vitamin B_(12)의 變化와 Pseudomonas sp.의 Vitamin B_(12) 生産性에 관하여

黃圭贊,朴啓仁 경희대학교 부설 식량자원개발연구소 1986 硏究論文集 Vol.7 No.-

This study was to investigate the changes of microflora, sugar, pH, and vitamin B_(12) during the aging of fermented vegetables such as Tongkimchi and Dongchimi and the productivity of vitamin B_(12) by Pseudomonas denitrificans D-2-1 selected as vitamin B_12 producing bacteria from Dongchimi. The main bacteria responsible for Tongkimchi fermertation were Lactobacillus plantarum, Leuconostoc mesenteroides and Streptococcus faecalis, but the main bacteria for Dongchimi fermentation were Leuconostoc mesenteroides and the genus Pseudomonas. In TongKimchi and Dongchimi sugar continuously decreased from ca 3% and pH reduced from 5.8 to 4.0 and less, but increased as the over-ripening progresses. Vitamin B_(12) content of TonKimchi was higher than those of Dongchimi at the early stage, but the opposite result at a later period Vitamin B_(12) content of TongKimchi and Dongchimi reached its maximum at the middle period. Pseudomonas denitrificans D-2- 1 cultured for 15 days on the media of radish juice produced vitamin B_12 up to 274 ng/㎖ and the strain cultured on the media of radish juice containing 1.5% NaCl produced vitamin B_12 of 261 ng/㎖. The strain cultured on the media of radish juice containing 3% NaCl and 0.1% L-glutamic acid as a nitrogen source produced vitamin B_(12) of 272ng/㎖. In contrast to the amount of vitamin B_(12)(245ng/㎖) obtained from the media in which L-glutamic acid was not added, the media containing L-glutamic acid increased the productivity of vitamin B_(12) up to 11%

The Methodological Concepts of Applied Statistics in Bussiness Forecasts

Chan-Ke Park 中央大學校 政經大學 應用統計學科 1982 統計學會誌 Vol.1982 No.8

형질전환에 의한 Ti Plasmid 의 Bradyrhizobium japonicum 에의 도입

박찬영,이계호,조무제,윤한대 한국농화학회 1987 Applied Biological Chemistry (Appl Biol Chem) Vol.30 No.4

Bradyrhizobium japonicum spheroplasts were prepared by culturing cells in the presence of glycine, follwed by treatment with lysozyme. The cells were examined by electron microscopy during the formation of spheroplast. Then Ti plasmid from Agrobacterium tumefaciens 15955 was introduced into Bradyrhizobium japonicum by glycine-lysozyme induced spheroplast transformation. After cell wall regeneration, transformants were selected by the ability of utilization of octopine. Transformation were received at a frequency of 1×10^(-7). The transformants obtained from spheroplast transformation harbored the introduced Ti plasmid, which was identified by agarose gel electrophoresis. Furthermore, the differences in their gene products were observed between the transformant and the recipient cell by two-dimensional polyacrylamide gel electrophoresis. The transformants which still possessed the same ability nodulate soybean (Glycine max.) as that of the original host strain, acquired the ability to induce tumor on Petunia hybrida like Agrobacterium, but formed the small crown galls in size compared to those of Agrobacterium tumefaciens.

한국전통누룩에 존재하는 사상균의 분리 동정 및 Amylolytic 효소 활성

박정웅,이계호,이찬용 한국산업미생물학회 1995 한국미생물·생명공학회지 Vol.23 No.6

전국에서 재래식 누룩 12개를 지역별로 수집하여 사상균을 분리, 동정한 결과 Absidia sp.가 Absidia corymbifera, Absidia ramosa, 종명은 확인되지 않은 Absidia spp. 등 총 78균주, Rhizopus spp.가 Rhizopus cohnii, Rhizopus arrhizus, Rhizopus oryzae 등 19균주, Circinella spp.가 8균주, Actinomucor sp.가 1균주, Aspergillus spp.가 Aspergillus oryzae, Aspergillus flavus, Aspergillus flavus var. columnaris, Aspergillus wentii, Aspergillus candidus, Aspergillus fumigatus, Aspergillus niger, Aspergillus foetidus 등 49균주, Clasdosporium spp.가 2균주, Botryotrichum spp.가 2균주, 따라서 총 159균주가 분리되었으며 수집지역과 누룩시료에 따라 착생된 사상균의 수와 분포는 많은 차이를 보였다. 사상균의 분포는 Absidia spp.가 모든지역의 누룩시료로 부터 가장높은 빈도로 분리 되었고 Aspergillus oryzae, Aspergillus condidus, Rhizopus cohnii가 비교적 고른분포를 보였으며 Penicillium sp.와 Mucor sp.는 분리되지 않았고 지금까지 보고된 바 없는 Actinomucor sp., Aspergillus foetidus, Botryotrichum sp., Cladosporium sp.가 분리되었다. 또한 분리균주의 amylase 활성과 pH 안정성 그리고 산생성능을 비교한 결과, A06(Aspergillus niger), F05(Aspergillus niger), J04(Aspergillus oryzae), J07(Aspergillus foetidus), D06(Rhizopus oryzae), F07(Rhizopus oryzae) 등이 우수하였다. Through the study of identification of filamentous molds isolated from 12 traditional Nuruk, collected from several regions in Korea, 78 strains of Absidia spp., including Absidia corymbifera, Absidia ramosa, and Absidia sp. of which specific names were not identified, 19 strains of Rhizopus spp. including Rhizopus cohnii, Rhizopus arrhizus, and Rhizopus oryzae, 8 strains of Circinella spp., 1 strain of Actinomucor sp., 49 strains of Aspergillus spp., including Aspergillus oryzae, Aspergillus flavus, Aspergillus flavus var. columnaris, Aspergillus wentii, Aspergillus candidus, Aspergillus fumigatus, Aspergillus niger, and Aspergillus foetidus, 2 strains of Cladosporium spp. and 2 strains Botryotrichum spp., etc. total 159 kinds of filamentous molds were isolated and identified. There were many differences in numbers and distributions of filamentous molds from each Nuruk according to their collected region. Absidia spp. were most frequently isolated from every Nuruk sample. Aspergillus oryzae, Aspergillus candidus, and Rhizopus cohnii showed even distribution. Penicillium sp. and Mucor sp. were not detected. Actinomucor sp., Aspergillus foetidus, Botryotrichum sp., and Cladosporium spp., which have not been reported by far, were found. Amylase activities, pH stability of amylase, and acid productivity of isolated strains were compared.

04 포스터 발표 : 환경 화학 분야(PC) ; PC-02 : 쪽파 중 Cyhalothrin과 Diflubenzuron의 잔류 특성

박효경 ( Hyo Kyoung Park ),노현호 ( Hyun Ho Noh ),이재윤 ( Jae Yun Lee ),김진찬 ( Jin Chan Kim ),정혜림 ( Hye Rim Jeong ),이정우 ( Jung Woo Lee ),진미지 ( Me Jee Jin ),권찬혁 ( Chan Hyeok Kwon ),손영욱 ( Yoeng Uk Son ),경기성 ( Ke 한국환경농학회 2015 한국환경농학회 학술대회집 Vol.2015 No.-

이 연구는 우리나라 대표 조미 채소인 쪽파 중 cyhalothrin(1% EC)과 diflubenzuron(25% WP)잔류특성을 구명하여 소면적 재배 작물의 농약에 대한 안전성을 확보하기 위하여 수행하였다. 포장시험은 쪽파 전문 재배 단지인 충남 예산군 소재 시설단지 내 농가 포장을 임차하여 수행하였으며, 수확 21일전 7일 간격 2회 살포구 등 약제 살포일을 달리하여 4개의 처리구(21-14, 14-7, 10-3,7-0)를 배치하였다. Cyhalothrin과 diflubenzuron은 각각 1,000과 2,500배 희석하여 200 L/10a의 액량으로 살포하였으며, 수확 당일 살포한 처리구는 약제 살포 3시간 경과 후 시료를 채취하였다. 쪽파 중 cyhalothrin과 diflubenzuron의 검출한계는 각각 0.004와 0.02 mg/kg이었으며, 회수율은 각각 92.7-99.3과 84.4-96.1%이었다. 쪽파 중 cyhalothrin의 수확 21일전과 7일전 2회 살포 처리구의 잔류량은 각각 0.014-0.019와 0.248-0.299 mg/kg이었으며, diflubenzuron의 경우 각각 0.38-0.59와2.22-2.89 mg/kg으로 수확일에 근접한 농약 살포가 수확물 중 최종 잔류량에 큰 영향을 미치는 것으로 판단되었다. Lambda-cyhalothrin은 약제 살포 후 빠르게 감소한다는 선행 연구 결과와 유사하였으며(Seenivasan 등, 2009), diflubenzuron의 경우 광분해에 안정하고 가수분해가 거의 없는 물리화학적 특성 때문에 잔류량이 높았다고 판단되었다.