Carbohydrate-containing Triton X-100 analogues for membrane protein solubilization and stabilization

Chae, Pil Seok,Wander, Marc J.,Cho, Kyung Ho,Laible, Philip D.,Gellman, Samuel H. The Royal Society of Chemistry 2013 Molecular bioSystems Vol.9 No.4

<P>Membrane protein manipulation is a challenging task owing to limited tertiary and quaternary structural stability once the protein has been removed from a lipid bilayer. Such instability can be overcome by embedding membrane proteins in detergent micelles formed from amphiphiles with carefully tuned properties. This study introduces a class of easy-to-synthesize amphiphiles, which are designated CGT (Chae's Glyco-Triton) detergents. Some of the agents are well suited for membrane protein solubilization and stabilization.</P> <P>Graphic Abstract</P><P>This study introduces a class of easy-to-synthesize amphiphiles that display favorable behaviour for membrane protein solubilization and stabilization. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3mb25584k'> </P>

식물추출물의 Tyrosinase 활성 억제효과

이정숙,채은경,계경채,문석식,조순장,김창덕,박장규,이증훈 大韓皮膚硏究學會 2003 大韓皮膚硏究學會誌 Vol.10 No.4

광주지역 영유아의 예방접종실태에 관한 조사연구

채경님 中央醫學社 1976 中央醫學 Vol.31 No.3

A survey on status of vaccination in children was carried out upon 314 housewives by questionnaire from September to December, 1975 in Mother-Children Health Center at Chonnam University Hospital and Health Center, Kwangju. The results obtained were as follows: 1. 55.4% of children received vaccination at general hospitals or private clinics, and 35. 0% of children at. Health Center. 2. The vast majority of respondents recognized the necessity of vaccination, and 88.5% of the subjects knew about the vaccination schedule. 3. The knowledge about various vaccination in order of the frequency was as follows: poliomyelitis(91.3%), small pox(90.7%), measles(85.7%); BCG(79.5%), and DPT(73.2%). 4. The frequency of the number of vaccinated children with various vaccines was as follows: poliomyelitis(84.7%), small pox( 82.2%), BCG(68. 20), .DPT (51. 6%), and measles(45. 9%).

내과 입원 환자에 대한 통계적 관찰

채경님 中央醫學社 1977 中央醫學 Vol.32 No.4

The interviews with 50 parents whose children are admitted to the S.N.U.H from August 1st 1976 to September 30th disclosed following results. The interview were carried on a predetermined questionnaire to evaluate the emotional reactions of parents and most of the children were pediatric patients. 1) The reaction of the parents to the disease of their children did not show any significant difference according to the age of children. 2) When their children were diseased, the reactions of the parents among different age groups did not show any significant difference. 3) The degrees of understanding of the parents on the diseases of their children did not show any significant difference, although the educational level of the parents were quite variable. 4) The differences between their social milieu also did not make any significant difference in the understand of their children's diseases. 5) - The most concerned subject of the parents was the recovery of their children. 6) Most of the parents are disappointed for the nurses to wake up their sleeping children to check vital signs and give injection, and not to share enough time for their children.

임의의 횟수의 휴가를 갖는 M^x/G/1 및 Geo^x/G/1 대기행렬의 분석

채경철,김남기,이호우 한국경영과학회 2002 韓國經營科學會誌 Vol.27 No.2

By using the arrival time approach of Chase et al. [6], we derive various performance measures including the queue length distributions (in PGFs) and the waiting time distributions (in LST and PGF) for both M^x/G/1 and Geo^x/G/1 queueing systems, both under the assumption that the server, when it becomes idle, takes multiple vacations up to a random maximum number. This is an extension of both Choudhury[7] and Zhang and Tian[11]. A few mistakes in Zhang and Tian are correscied and meaningful interpretations are supplemented.

가솔린機關의 室素酸化物 放出 低減을 위한 硏究

慶在浩 弘益大學校 1976 弘大論叢 Vol.8 No.-

In this paper, studies on the reduction in nitrogen oxide emmission is made by analysis with exhaust gas in automobiles. In combining with oxygen in air, nitrogen oxide generated under the conditions of high temperature and pressure when combustion is conducted in cylinder. In order to reduce the quantity of this undesirable emissions, the following method is taken. ① It is neccessary for intake vaccuum pressure to increase and temperature of gas mixture to decrease. ② a study on reduction of NOχ by water injection in cylinder ③ EGR control system.

전류밀도 향상을 위한 금속 산화물 피막 제조에 관한 연구

채경선,유국현 동국대학교 자연과학연구원 2000 자연과학연구 논문집 Vol.5 No.-

구조물의 부식을 저감시키기 위한 재료로 사용되려면 전기 전도성이 좋아야 하며, 여러 산업환경에 적용될 수 있도록 내식성이 뛰어나야한다. 이리 듐-루테늄 산화물 피막은 이러한 성질을 만족시키는 물질로, 이들 산화물 피막 의 성질은 대개 양극 제조 공정에 의해 크게 좌우되므로 본 연구에서는 전류 밀도 향상을 위한 제조 공정을 최적화하고자 하였다. Dip-coating법으로 전도성 코팅층인 이리듐-루테늄 산화물 피막을 기지금속인 티타늄 위에 피복 시키는데, 이 코팅층의 내식성 향상을 위해서 다구치 방법을 도입하여 최적 공정 조건을 구하였다. 이때 사용된 변수는 전처리 방법, 열처리 온도, 열처리시 주입 가스량, 열처리 시간으로 이들 변수를 3가지로 변화시켜 실험하였다. 실험결과 이리듐-루테늄 산화물 피막 제조시 기판위에 티타늄산화물 중간층을 plasma spray법으로 제조하고, 열처리 온도 450℃, 주입가스량 3sccm, 열처리시간을 120분으로 하였을 때 방식전류밀도가 가장 우수한 코팅층(활성화 코팅층)을 얻었다.

이상적인 측정환경이 아닌 장소에서 Headphone Audiocup의 부착유무가 현장 근로자의 청력검사에 미치는 영향

채경석,구정완,이원철 가톨릭대학교 산업의학센터 2000 韓國의 産業醫學 Vol.39 No.4

The purpose of this study was to assess the effect of headphone audiocup. The subjects were composed of workers of noise-exposed population. The noise level of workplace was over 85 dB. They were examined otoscopic test and audiometry of 1,000 Hz and 4,000 Hz. The data were analysed according to wearing audiocups for 612 subjects in -59 years of age and major results are as follows : 1.Hearing thresholds of group audiocups were significantly decreased than that of non-audiocups in -19, 20-29, 40-49 and 50-59 years of age on left ear of male workers and in -19, 20-29 years of age on left ear of female for 1,000 Hz. 2.Hearing thresholds of group audiocups were significantly decreased than that of non-audiocups in 20-29, 40-49 and 50-59 years of age on right ear of male and in -19, 20-29 and 40-49 years of age on right ear of female for 1,000 Hz. 3.Hearing thresholds of group audiocups were significantly decreased than that of non-audiocups in 20-29, 30-39 and 40-49 years of age on left ear of male worriers and in 20-29 years of age on left ear of female for 4,000 Hz. 4.Hearing thresholds of group audiocups were significantly decreased than that of non-audiocups in 20-29, 30-39 and 50-59 years of age on right ear of male and in 20-29 and 40-49 years of age on right ear of female for 4,000 Hz.

전기천공법에 의한 Bacillus brevis P176-2의 형질전환

채기수,엄경일 東亞大學校附設基礎科學硏究所 1993 基礎科學硏究論文集 Vol.10 No.1

균체외단백질 생성균 Bacillus brevis P176-2 균주를 pUB110 plasmid DNA로 전기천공법에 의한 형질전환을 시도하여 본 방법의 유효성과 최적조건을 검토하였다. 본 실험에 사용한 전기천공 장치로 전장강도 12.5kV/㎝, capacitor 용량 7.3㎌의 조건하에서 고전압 pulse를 세포에 가했을 때 가장 높은 형질전환율(1.26× 10⁴/㎍ plasmid DNA)을 나타내었으며 세포 생존율은 10% 정도이었다. 세포의 생육도가 ??에서 약 1.2정도로 증식하였을 때 세포를 수확하여 형질전환 시키는 것이 가장 좋았으며 그 이상의 생육도에서는 세포의 생존율은 증가되었지만 형질전환율은 낮아졌다. 전강강도 12.5kV/㎝, capacitor용량 7.3㎌ 조건의 pulse를 세포에 2회 연속적으로 가할 때 최대의 형절전환율(2.40× 10⁴/㎍ plasmid DNA)을 보였다. 세포의 생존과 pulus의 지속시간에 영향을 주는 electroporation burrer는 7 mM HEPES(pH 7.4), 272mM sucrose 및 1mM MgCl₂을 함유하는 buffer를 사용하여 세포를 수확하고 현탁하여 형질전환할 때 형질전환율과 세포 생존율이 가장 높았다. 세포현탁액에 첨가되는 plasmid DNA의 농도가 10㎍/ml일 때 까지는 농도에 비례하여 형질전환율이 높아졌으나 그 상의 농도에서는 비례하지 않았다. The optimum conditions and mechanisms for the plasmid-mediated genetic transformation of intact cells of bacillus brevis P176-2, an extracellular protein producing bacterium, by electroporation were investigated. It was found that pUB110 plasmid DNA can be introduced into intact bacterial cells by electroporation. The frequency of transformation by this electroporation system depended upon the initial electric field strength, the capacity of the clectric discharge capacitor, growth stage, number of successive pulses and composition of electroporation buffer. It was effective for transformation that cells were harvested, washed and resuspended with HSM[7mM HEPES(pH 7.4), 272mM sucrose, 1mM MgCl₂] electroporation buffer when cell growth was attained to 1.2 at ?? A maximum frequency of transformation of 2.40× 10⁴ transformants per ㎍ plasmid DNA was obtained by two succesive pulses with an initial electric field strength of 12.5kV/㎝ and with a capacitance of 7.3㎌. Key words : Bacillus breves, pUB110, plamid DNA, transformation, electroporation.

素問·氣交變大論에 對한 硏究

蔡英?,鄭憲瑩,琴坰樹,朴炅 대한한의진단학회 1998 大韓韓醫診斷學會誌 Vol.2 No.2

The contents of Ki Kyo Byun Dae Ron(氣交燮大論) are explained on the basis of the so-called "Five elements motion and six kinds of natural factorsc(五運大氣)" theory. The relevant adaptation of the human body to natural enviroment is one of the basic theoties in Oriental medicine, according to which the physical structure and physiological phenomena of human body as well as the physiological changes are in adaptative confirmity with the variations of the natural environment, and hence in diagnosis and treatment the influences of environmental factors such as climatic conditions, geographical localities, etc., should be considered. The theory of Fve Circuit Phases and Six Atomsphric Influences is based on this thought. Summeriiing the contents of the Ki Kyo Byun Dae Ron(氣交燮大論) in reference to the contents of successive generation note, the contents were as follows; What attract our attention in chapter Ⅰ are climatic changes and pathologic explanations on the diseases which are invited by a certain excessive circuit phase. What attract our attention in chapter Ⅱ are climatic changes and pathologic explanations on the diseases which are invited by a certain insufficient circuit phase. The main point of chapter Ⅲ is the promoting and counteracting relations of the five circuit phases taken as a whole, which forms a self-limiting balanced process. What attract our attention in chapter Ⅳ, Ⅴ is the change of five planets brigtness which is invited by excessive or insufficient circuit phase.