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Ra, Chae Hun,Jung, Jang Hyun,Sunwoo, In Yung,Jeong, Gwi-Taek,Kim, Sung-Koo Springer-Verlag 2015 BIOPROCESS AND BIOSYSTEMS ENGINEERING Vol.38 No.6
<P>A total monosaccharide concentration of 59.0 g/L, representing 80.1 % conversion of 73.6 g/L total fermentable sugars from 160 g dw/L G. amansii slurry was obtained by thermal acid hydrolysis and enzymatic hydrolysis. Subsequent adsorption treatment using 5 % activated carbon with an adsorption time of 2 min was used to prevent the inhibitory effect of 5-hydroxymethylfurfural (HMF) > 5 g/L in the medium. Ethanol production decreased with increasing salt concentration using C. tropicalis KCTC 7212 non-acclimated or acclimated to a high concentration of salt. Salt concentration of 90 psu was the maximum concentration for cell growth and ethanol production. The levels of ethanol production by C. tropicalis non-acclimated or acclimated to 90 psu high-salt concentration were 13.8 g/L with a yield (Y-EtOH) of 0.23, and 26.7 g/L with Y-EtOH of 0.45, respectively.</P>
Ra, Chae Hun,Kang, Chang-Han,Kim, Na Kyoung,Lee, Choul-Gyun,Kim, Sung-Koo Elsevier 2015 Renewable energy Vol.80 No.-
<P><B>Abstract</B></P> <P>A two-stage culture strategy was used for maximum biomass production under nutrient-sufficient conditions, followed by cultivation under low-salt stress, to cause the accumulation of oil in the biomass. Controlled conditions of nitrate, salt concentration, and time to exposure to stress were optimized for oil production with four species of microalgae, <I>Isochrysis galbana</I>, <I>Nannochloropsis oculata</I>, <I>Dunaliella salina</I>, and <I>Dunaliella tertiolecta</I>. Using conditions with addition of nitrate to 24.0 mg/L, <I>I. galbana</I> and <I>N. oculata</I> showed higher biomass productions than <I>D. salina</I> and <I>D. tertiolecta</I>. The oil contents of the microalgae increased from 24.0% to 47.0% in <I>I. galbana</I> with 10 psu for 2 days, from 17.0% to 29.0% in <I>N. oculata</I> with 0 psu for 3 days, from 22.0% to 43.0% of <I>D. salina</I> with 10 psu for 1 day, and from 23.0% to 40.0% (w/w) in <I>D. tertiolecta</I> with 0 psu for 2 days as the second stage culture with low-salt stress. Thus, <I>I. galbana</I> could be a suitable candidate microalga for oil production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A two-stage culture was used for algae biomass production and for oil accumulation. </LI> <LI> Oil accumulation under low-salt stress enhanced overall oil productivity. </LI> <LI> The highest oil content was 47.0% with <I>I. galbana</I> at 10 psu salt stress for 2 days. </LI> <LI> <I>I. galbana</I> could be a suitable candidate microalga for oil production. </LI> <LI> Algae cell growth kinetics was determined by Monod equation. </LI> </UL> </P>
Ra, Chae Hun,Jung, Jang Hyun,Sunwoo, In Young,Kang, Chang Han,Jeong, Gwi-Taek,Kim, Sung-Koo The Korean Society for Microbiology and Biotechnol 2015 Journal of microbiology and biotechnology Vol.25 No.6
The objective of this study was to optimize the slurry contents and salt concentrations for ethanol production from hydrolysates of the seaweed Eucheuma spinosum. A monosaccharide concentration of 44.2 g/l as 49.6% conversion of total carbohydrate of 89.1 g/l was obtained from 120 g dw/l seaweed slurry. Monosaccharides from E. spinosum slurry were obtained by thermal acid hydrolysis and enzymatic hydrolysis. Addition of activated carbon at 2.5% (w/v) and the adsorption time of 2 min were used in subsequent adsorption treatments to prevent the inhibitory effect of HMF. The adsorption surface area of the activated carbon powder was 1,400-1,600 m<sup>2</sup>/g and showed selectivity to 5-hydroxymethyl furfural (HMF) from monosaccharides. Candida tropicalis KCTC 7212 was cultured in yeast extract, peptone, glucose, and high-salt medium, and exposed to 80, 90, 100, and 110 practical salinity unit (psu) salt concentrations in the lysates. The 100 psu salt concentration showed maximum cell growth and ethanol production. The ethanol fermentations with activated carbon treatment and use of C. tropicalis acclimated to a high salt concentration of 100 psu produced 17.9 g/l of ethanol with a yield (Y<sub>EtOH</sub>) of 0.40 from E. spinosum seaweed.