The Association of Treponema denticola with Human Gingival Keratinocytes

Camargo, Paulo M.,Wolinsky, Lawrence E.,Park, No-Hee Korean Academy of Oral Biology and the UCLA Dental 1996 International Journal of Oral Biology Vol.21 No.1

The adhesion of Treponema denticola to human gingival kerationcytes was quantified amd examined with electron microscopy. This study was undertaken as an initial step in defining the potential role of oral treponemes in the initiation and progression of periodontal disease. The adhesion of ^35S-labeled T. denticola to gingival keratinocytes was very similar to the adhesion to human gingival fibroblasts. Heat-treatment of T. denticola resulted in a significant reduction in bacterial adhesion to the keratinocytes. Treatment of T. denticola cells with proteinase K prior to incubation with keratinocytes resulted in a dose-dependent adhesion reduction (41% at 0.5 mg/ml of proteinase K). Treatment of the keratinocytes with neuraminidase prior to incubation with T. denticola led to a 29% increase in bacterial adhesion to gingival keratinocytes over control. Exposure of T. denticola to 50 mM D-galactose, 50 mM methyl-β-D-galactopyranoside and 50mM D-N-acetyl galactosamine reduced subsequent T. denticola adhesion to gingival keratinocytes by up to 70%. The adhesion of T. denticola was significantly inhibited by heat-inactivated rabbit serum. Electron microscopic examination revealed a "body"-oriented localization of T. denticola at the intercellular junctions of the gingival keratinocyte monolayer. No T. denticola cells were seen penetrating the cell membranes or within the cytoplasm of the keratinocytes. The results suggest that T. denticola adhesion to human gingival keratinocytes may be mediated through carbohydrate-protein interactions localized at the intercellular junctional region of the gingival keratinocyte monolayers.

Concentrations of Cyclosporin A and Metabolites in the Gingival Tissues of Transplant Patients

Camargo, Paulo M.,Wolinsky, Lawrence E.,McBride, James H.,Barrie Kenney, E.,Carranza, Fermin A.,Klokkevold, Perry R.,Robert Ettinger,Jennifer Marik Korean Academy of Oral Biology and the UCLA Dental 1999 International Journal of Oral Biology Vol.24 No.4

Cyclosporin A (CyA) is a potent immunosuppressive drug used to avert rejection following human organ transplant surgery and as therapy for various atuoimmune diseases. One complication associated with CyA therapy is gingival enlargement observed post transplantation. The purpose of this study was to determine if concentrations of CyA and its metabolites in the gingival tissues of patients presenting with gingival enlargement (responders) were higher than those in patients without enlargement (nonresponders). Ten organ transplant recipients (9 kidney, 1 liver) woh were receiving CyA for at least 6 months were classified clinically as responders and nonresponders. Site selection for biopsy was based on the degree of gingival enlargement and all biopsy sites presented minimal plaque and gingival inflammation. One biopsy was taken from each of the nonresponders and two were taken from the responders (one enlarged and one nonenlarged area). CyA concentrations in the biopsies were determined by HPLC and CyA plus metabolites was determined by Abbott TDx. Mean results demonstrated that enlarged and nonenlarged sites in responders presented with similar concentrations of CyA and CyA plus metabolites No statistically significantdifferences were found between the concentration of cya or cya plus metabolites in nonenlarged sites of responders when compared to nonenlarged sites of nonresponders, although nonresponders demonstrated higher values. Finally, enlarged sites in responders showed lower but not statistically significant concentrations of CyA or CyA plus metabolites than nonenlarged sites in nonresponders. This study demonstrates that the concentration of CyA and CyA plus metabolites in the gingival tissues of individuals taking the drug is not the single determinant factor in the development of gingival enargement.

Improved survival of gastric cancer with tumour Epstein–Barr virus positivity: an international pooled analysis

Camargo, M Constanza,Kim, Woo-Ho,Chiaravalli, Anna Maria,Kim, Kyoung-Mee,Corvalan, Alejandro H,Matsuo, Keitaro,Yu, Jun,Sung, Joseph J Y,Herrera-Goepfert, Roberto,Meneses-Gonzalez, Fernando,Kijima, Yuk BMJ Publishing Group Ltd 2014 Gut Vol.63 No.2

<P><B>Background and objective</B></P><P>About 9% of gastric carcinomas have Epstein–Barr virus (EBV) in the tumour cells, but it is unclear whether viral presence influences clinical progression. We therefore examined a large multicentre case series for the association of tumour EBV status with survival after gastric cancer diagnosis, accounting for surgical stage and other prognostic factors.</P><P><B>Methods</B></P><P>We combined individual-level data on 4599 gastric cancer patients diagnosed between 1976 and 2010 from 13 studies in Asia (n=8), Europe (n=3), and Latin America (n=2). EBV positivity of tumours was assessed by in situ hybridisation. Mortality HRs for EBV positivity were estimated by Cox regression models stratified by study, adjusted for distributions of sex (71% male), age (mean 58 years), stage (52% tumour-node-metastasis stages III or IV), tumour histology (49% poorly differentiated, 57% Lauren intestinal-type), anatomic subsite (70% non-cardia) and year of diagnosis. Variations by study and continent were assessed using study-specific HRs for EBV positivity.</P><P><B>Results</B></P><P>During median 3.0 years follow-up, 49% of patients died. Stage was strongly predictive of mortality, with unadjusted HRs (vs stage I) of 3.1 for stage II, 8.1 for stage III and 13.2 for stage IV. Tumour EBV positivity was 8.2% overall and inversely associated with stage (adjusted OR: 0.79 per unit change). Adjusted for stage and other confounders, EBV positivity was associated with lower mortality (HR, 0.72; 95% CI 0.61 to 0.86), with low heterogeneity among the study populations (p=0.2). The association did not significantly vary across patient or tumour characteristics. There was no significant variation among the three continent-specific HRs (p=0.4).</P><P><B>Conclusions</B></P><P>Our findings suggest that tumour EBV positivity is an additional prognostic indicator in gastric cancer. Further studies are warranted to identify the mechanisms underlying this protective association.</P>

No Relationship between the Amount of DNA Damage and the Level of hMLH1 and RASSF1A Gene Expression in Bladder Cancer Cells Treated with Cisplatin and Gemcitabine

de Camargo, Elaine Aparecida,da Silva, Glenda Nicioli,Gobette, Camila Pereira,de Castro Marcondes, Joao Paulo,Salvadori, Daisy Maria Favero Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Tumor response to antineoplastic drugs is not always predictable. This is also true for bladder carcinoma, a highly recurrent neoplasia. Currently, the combination of cisplatin and gemcitabine is well accepted as a standard protocol for treating bladder carcinoma. However, in some cases, this treatment protocol causes harmful side effects. Therefore, we investigated the roles of the genes TP53, RASSF1A (a tumor suppressor gene) and hMLH1 (a gene involved in the mismatch repair pathway) in cell susceptibility to cisplatin/gemcitabine treatment. Two bladder transitional carcinoma cell (TCC) lines, RT4 (wild-type TP53) and 5637 (mutated TP53), were used in this study. First, we evaluated whether the genotoxic potential of cisplatin/gemcitabine was dependent on TP53 status. Then, we evaluated whether the two antineoplastic drugs modulated RASSF1A and hMLH1 expression in the two cell lines. Increased DNA damage was observed in both cell lines after treatment with cisplatin or gemcitabine and with the two drugs simultaneously, as depicted by the comet assay. A lack of RASSF1A expression and hypermethylation of its promoter were observed before and after treatment in both cell lines. On the other hand, hMLH1 downregulation, unrelated to methylation status, was observed in RT4 cells after treatment with cisplatin or with cisplatin and gemcitabine simultaneously (wild-type TP53); in 5637 cells, hMLH1 was upregulated only after treatment with gemcitabine. In conclusion, the three treatment protocols were genotoxic, independent of TP53 status. However, cisplatin was the most effective, causing the highest level of DNA damage in both wild-type and mutated TP53 cells. Gemcitabine was the least genotoxic agent in both cell lines. Furthermore, no relationship was observed between the amount of DNA damage and the level of hMLH1 and RASSF1A expression. Therefore, other alternative pathways might be involved in cisplatin and gemcitabine genotoxicity in these two bladder cancer cell lines.

Effects of a bleaching agent on properties of commercial glass-ionomer cements

de Camargo, Fernanda Lucia Lago,Lancellotti, Ailla Carla,de Lima, Adriano Fonseca,Martins, Vinicius Rangel Geraldo,de Souza Goncalves, Luciano The Korean Academy of Conservative Dentistry 2018 Restorative Dentistry & Endodontics Vol.43 No.3

Objectives: This study evaluated the effects of a bleaching agent on the composition, mechanical properties, and surface topography of 6 conventional glass-ionomer cements (GICs) and one resin-modified GIC. Materials and Methods: For 3 days, the specimens were subjected to three 20-minute applications of a 37% $H_2O_2$-based bleaching agent and evaluated for water uptake (WTK), weight loss (WL), compressive strength (CS), and Knoop hardness number (KHN). Changes in surface topography and chemical element distribution were also analyzed by energy-dispersive X-ray spectroscopy and scanning electron microscopy. For statistical evaluation, the Kruskal-Wallis and Wilcoxon paired tests (${\alpha}=0.05$) were used to evaluate WTK and WL. CS specimens were subjected to 2-way analysis of variance (ANOVA) and the Tukey post hoc test (${\alpha}=0.05$), and KH was evaluated by one-way ANOVA, the Holm-Sidak post hoc test (${\alpha}=0.05$), and the t-test for independent samples (${\alpha}=0.05$). Results: The bleaching agent increased the WTK of Maxxion R, but did not affect the WL of any GICs. It had various effects on the CS, KHN, surface topography, and the chemical element distribution of the GICs. Conclusions: The bleaching agent with 37% $H_2O_2$ affected the mechanical and surface properties of GICs. The extent of the changes seemed to be dependent on exposure time and cement composition.

Co-Composting of Solid Waste Organic Urban with Sludge

Gloria Lucia Camargo Millán 한국외국어대학교 국제지역연구센터 2017 국제지역연구 Vol.21 No.2

Sludge from wastewater treatment plants (WWTP) in municipalities of Boyacá has low levels of heavy metals as required by Colombian regulations, which allows a great potential for use as soil improvers. These, when used in the production of ornamental crops or in the recovery of soils, give rise to a positive environmental impact. This project developed a field trial of co-composting municipal solid organic waste with WWTP sludge in a municipality of Boyacá, finding the optimum proportion of these materials, characterizing the two raw materials and the most suitable mixture. Currently, the requirements for registering the biofertilizer or soil improver are being reviewed before national entities (Super intendency of industry and trade, and Colombian Institute of Agriculture (ICA) with a view to its future commercialization.

Effects of a bleaching agent on properties of commercial glass-ionomer cements

Fernanda Lúcia Lago de Camargo,Ailla Carla Lancellotti,Adriano Fonseca de Lima,Vinícius Rangel Geraldo Martins,Luciano de Souza Gonçalves 대한치과보존학회 2018 Restorative Dentistry & Endodontics Vol.43 No.3

Objectives: This study evaluated the effects of a bleaching agent on the composition, mechanical properties, and surface topography of 6 conventional glass-ionomer cements (GICs) and one resin-modified GIC. Materials and Methods: For 3 days, the specimens were subjected to three 20-minute applications of a 37% H2O2-based bleaching agent and evaluated for water uptake (WTK), weight loss (WL), compressive strength (CS), and Knoop hardness number (KHN). Changes in surface topography and chemical element distribution were also analyzed by energy-dispersive X-ray spectroscopy and scanning electron microscopy. For statistical evaluation, the Kruskal-Wallis and Wilcoxon paired tests (ɑ = 0.05) were used to evaluate WTK and WL. CS specimens were subjected to 2-way analysis of variance (ANOVA) and the Tukey post hoc test (α = 0.05), and KH was evaluated by one-way ANOVA, the Holm-Sidak post hoc test (ɑ = 0.05), and the t-test for independent samples (ɑ = 0.05). Results: The bleaching agent increased the WTK of Maxxion R, but did not affect the WL of any GICs. It had various effects on the CS, KHN, surface topography, and the chemical element distribution of the GICs. Conclusions: The bleaching agent with 37% H2O2 affected the mechanical and surface properties of GICs. The extent of the changes seemed to be dependent on exposure time and cement composition.

Experimental and Theoretical Study of the Heat Transfer Coefficient in Horizontal Two-Phase Intermittent Flow

R. M. T. Camargo,A . C. Bannwart,F. A. Franca 대한기계학회 1993 International Symposium on Transport Phenomena in Vol.2 No.1

The Effect of Auricular and Systemic Acupuncture on the Electromyographic Activity of the Trapezius Muscle with Trigger Points—A Pilot Study

Patrı´cia Silva de Camargo,Carla Rigo Lima,Maria Luiza de Andrade E Rezende,Adriana Teresa Silva Santos,Joa˜o Wagner Rodrigues Hernandez,Andre´ia Maria Silva 사단법인약침학회 2018 Journal of Acupuncture & Meridian Studies Vol.11 No.1

The purpose of this study was to analyze and compare intra and intergroup the immediate effect of the auricular and LR8 systemic acupuncture on the electromyographic activity of the trapezius with the trigger points. This is an experimental clinical trial; 40 people were split in 4 distinct groups (n = 10): GI mustard seed application in the auricular acupoint; GII bilateral needle application in the LR8 acupoint; GIII combination of the techniques; GIV/Control Group mustard seed application in an acupoint not linked to the muscle tension. The EMG was used to assess the muscle contraction for 5 seconds during the resting time and during the isometric contraction time. The EMG signal was first collect without the acupuncture intervention; then both techniques were applied for 5 minutes; and the EMG was collected again right after these applications. The Shapiro-Wilk test was used, the t test was paired with the Wilcoxon test to the intragroup comparison; One-way analysis of variance test for intergroup comparison. There was no statistical difference in the intragroup comparison for the groups. The same happened to the intergroup comparison before and after application. Systemic and auricular acupuncture did not promote immediate changes in the EMG activity of the trapezius muscle in individuals with MTrPs.

日本內 外國人投資企業의 R&D 活動 및 環境

Justin L. Bloom,Orlando Camargo,김기국(번역자) 과학기술정책연구원 1992 과학기술정책 Vol.- No.22