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RISS 인기검색어
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- 저자 : Cahe, Yang Seok (검색결과 6 건)
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배재근,심혁성,김혜경 서울産業大學校 産業大學院 1997 大學院論文集 Vol.5 No.-
생활계에서 배출되는 분뇨 및 하수처리장 등에서 발생되는 농축슬러지는 고농도폐수로서 그 처리에 많은 문제점을 안고 있다. 현재는 호기성소화 및 혐기성소화방식에 의하여 처리하고 있으나, 처리효율이 아직까지 낮은 상태이다. 이러한 문제점을 개선하기 위하여 여러 처리방법이 개발되고 있으나, 최근에 주목받고 있는 처리방법이 고온호기성소화방식이다. 고온호기성소화방식은 고열성세균 즉 고온에서 서식하는 미생물을 이용하여 처리하는 방식으로 이전에는 제어기술이 부족하여 응용화되지 않았으나, 최근에는 각종 제어기술이 확립되면서 점차 응용의 가능성이 밝혀지고 있다. 본 연구에서는 현재 처리상 문제로 되어 있는 분뇨의 처리실태를 조사하고, 실제 고온호기성소화공정에 의하여 유기물의 분해정도를 측정하였으며, 또한 이 공정의 효율을 증대시키는 방안으로서 미생물에 초점을 두고 미생물의 활성 및 그 이용의 가능성에 대하여 검토하였다. 기존 처리공정인 액상부식조에서 COD cr, COD Mn, BOD, TS 등의 제거율이 각각 31.3%, 48.9%, 62.9%, 4.08%로 나타났으나, 고온호기성소화장치로 운전한 결과, COD cr, COD Mn, BOD, TS, SS 등의 제거율은 각각 35%, 42%, 68%, 30%, 65%를 나타났다. 즉 최종처리수의 농도값에는 차이가 없으나, 체류시간 대폭 단축된다는 점에서 고온호기성소화가 유용한 것으로 판단되었다. 또한 고온에서 반응이 이루어지므로 대장균군의 사멸효과가 더 크다는 면에서 기존의 액상부식조와 비교할 때 우위에 있다는 것도 확인할 수 있었다. 고온호기성 미생물을 분리하고 미생물의 활성을 검토한 결과, 활성이 뛰어난 균주가 최종적으로 3종이 분리되었으며, 그 중에서도 사상성세균인 CA균의 활성이 뛰어나, 이 균의 특성을 면밀하게 분석하여, 고온호기성소화장치와 접목시킨다면, 유기물의 분해효율을 향상시킬 수 있는 결과가 얻어질 것으로 예측되었다. The most common maures wastewater disposal operations is the aerobic digestion, but with occupy much area, increasing treatment period, more stringent regulatory requirement is rapidly becoming cost prohibitive and impractical. Thermophilic aerobic digestion system sometimes referred to as liquid composting, is proprietary process which generates heat via the aerobic metabolism of organic matter. Under precisely controlled operating conditions the thermophilic bacteria thrive. This biological process will give rise the true thermophilic organism and generate sufficients heat to make the system self-sustaining in the thermophilic range. In comparison with the removal efficiency of Aerobic digestion process which is operating at K-environmental business place(COD cr 31.3%, COD Mn 48.9%, BOD 62.9%, TS 4.08%), the removal efficiencies of Thermophilic aerobic digestion process were COD cr 35%, COD Mn 42%, BOD 68%, TS 30%. And soluble COD cr, COD Mn removal efficiency were 66% and 49% in AD process and Pilot Scale, respectively. These results of TAD were seem nearly same but HRT of Ad process is 20days and HRT of Pilot Scale Plant is 8days compare with these two HRT, we can know the efficiency treatment of Pilot Scale is much higher than AD process. Moreover because of Pilot Scale Plant is operated at high temperature the destruction efficiency of E. Coli is much better Finally, we separate Thermophilic Aerobic microorganism to observe organic material degradation ability. so we confirm that degradation ability of to be inoculated microorganism is much higher than not to be inoculated.
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Cahe, Yang Seok,Hedley-Whyte, E. Tessa 고려대학교 의과대학 1996 고려대 의대 잡지 Vol.33 No.2
Flow cytometric analysis was applied to embedded tissue to measure the proliferative activity and the DNA ploidy of sixteen recurrent and seventeen nonrecurrent pituitary adenomas. The results were compared with data from a previous study which demonstrated that proliferationg cell nuclear antigen (PCNA) labelling index was higher in recurrent adenomas than in nonrecurrent adenomas. Flow cytometric analysis as a tool for predicting aggressive behavior has been useful in a variety of human tumors, however its prognostic value in pituitary adenoma is controversial. Therefore we decided to explore the relationship of the results of flow cytometry and proliferating cell nuclear antigen labelling indices with the prognosis of pituitary adenomas. Three out of 16 recurrent adenomas and five out of 17 nonrecurrent adenomas demonstrated a DNAaneuploid pattern. All the nonfunctional recurrent adenomas had a diploid pattern, while only 40% of the functional recurrent adenomas had a diploid pattern. The G0/Gl phase fraction was higher in the recurrent adenomas than in the non- recurrent ones. (p=0.0005) In contrast, the S phase fraction and the coefficient of variation were 1 higher in the nonrecurrent adenomas (5.9 1.0%, 7.0 0.75, respectively) than in the recurrent ones(2.5 0.6%, 4.0 0.2%, respectively) (P=0. 003 and p=0. 001, respectively). The proliferating cell nuclear antigen lavelling indices were higher in the recurrent adenomas (18.9 4.5%) than in the nonrecurrent adenomas (2.6 1.6%) (P=0.003). The S phase of flow cytometry correlated weakly with the proliferating cell nuclear antigen labelling indices when the recurrent and the nonrecurrent adenomas were considered as one group. (r=-0. 356, p=0.033) But no significant correlations were observed when the groups of recurrent (r=0.311, p=0.195) and nonrecurrent tumors (r=-0.019, P=0. 942) were compared separately. The results of flow cytometric analysis suggests that recurrent adenomas may have a higher proportion of cells in the presynthetic phase than the nonrecurrent adenomas. This study suggests that flow cytometric analysis is of limited value in predict- ing recurrence of pituitary adenomas.
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Methyl Orange에 의한 Benzalkonium Chloride의 Ion Pair 抽出 吸光度定量
金永洙,白采善,崔宗煥 조선대학교 약학연구소 1989 藥學硏究誌 Vol.11 No.1
Phthalein and azo dyes have been used by forming ion pair agents. Primary, secondary and tertiary amines in aliphatic amines have been fully examined, but aliphatic quaternary amines or their derivatives have not been researched satisfactorily until now. In this thesis, the author investigated the application of extraction method of organic solvents in forming ion pair between quaternary ammonium salts, and methyl orange (MO). In the process of laboratory work, MO and benzalkonium choride(BKC) were mixed and added the buffer solution to 10㎖, and then the solution was shaken for 5minutes. The maximum absorption wavelength of the above stable solution which was extracted with dichloromethane was 420nm. The maximum absorption wavelength of MO aqueous solution was 464nm, and it shifted 44nm toward the long wavelength from 464nm. In this experiment, the most suitable pH range was 2∼8. Dichloromethane was the best of all other solvents as than 1.2-dichloroethane, chloroform, benzene, nitrobenzene and carbon tetrachloride in the extraction solvents of ion pair which was formed between MO and BKC. However, MO has never been extracted with any kinds of solvents either. By using the method of continuous variation, the author investigated that the mole ratio of reaction products formed between MO and BKC was 1:1 ratio. When made the BKC calibration curve to MO in best experiment condition, the Lambert-Beer's law was obeyed in the range of BKC. concentration 3×10^-5∼1.0×10^-4 M.
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