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Kong, Byung-Seon,Geng, Jianxin,Jung, Hee-Tae Royal Society of Chemistry 2009 Chemical communications Vol.2009 No.16
<P>Layer-by-layer films comprised of alternating graphene and gold nanoparticle layers are readily produced by the two-step procedure involving the use of vacuum filtration of a reduced graphene oxide solution to fabricate the graphene thin film on the quartz substrate, followed by gold nanoparticle formation by spontaneous reduction of gold ions in a gold salt solution on the graphene films.</P> <P>Graphic Abstract</P><P>Layer-by-layer films comprised of alternating graphene and gold nanoparticle layers are readily produced by the vacuum filtration of a reduced graphene oxide solution and the spontaneous reduction of gold ions on the graphene films. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b821920f'> </P>
Kong, Hyun-Gi,Lee, Hyoung-Ju,Bae, Ju-Young,Kim, Nam-Hee,Moon, Byung-Ju,Lee, Seon-Woo The Korean Society of Plant Pathology 2010 Plant Pathology Journal Vol.26 No.3
Spatial and temporal distribution of Bacillus licheniformis N1 was investigated over time on the leaves, petioles and crowns of the strawberry plants. Bacterial population on the strawberry plants was quantified over time by selective plating. Bacterial population of N1 containing a plasmid pWH43G carrying green fluorescent protein (GFP) declined relatively faster on the plant surface as compared to the Strain N1 itself. However, this result was found to be enough to utilize the strain to visualize bacterial colonization on the plant surface. When B. licheniformis N1 was treated together with Silwet L-77 at 0.03%, the bacterial population on plant surface persisted for up to 7 days. B. licheniformis N1 (pWH43G) containing Silwet L-77 was applied on the strawberry plants and the GFP expressing bacteria were visualized by confocal laser scanning microscopy. Bacterial persistence was also investigated in a growth chamber and in a plastic house after N1 bioformulation treatment on the strawberry plant. The Strain N1 colonized three different tissues well and persisted over 3 to 5 days on the strawberry plants. They formed bacterial aggregates on plant surfaces for at least 3 days, resulting in a biofilm to resist fluctuating plant surface environment. However, the bacterial persistence dramatically declined after 7 days in all tested tissues in a plastic house. This study suggest that B. licheniformis N1 colonizes the strawberry plant surface and persists for a long time in a controlled growth chamber, while it can not persist over 7 days on the plant surface in a plastic house.
Kong, Hyun-Gi,Kim, Jin-Cheol,Choi, Gyoung-Ja,Lee, Kwang-Youll,Kim, Hyun-Ju,Hwang, Eul-Chul,Moon, Byung-Ju,Lee, Seon-Woo The Korean Society of Plant Pathology 2010 Plant Pathology Journal Vol.26 No.2
Bacillus licheniformis N1, previously developed as a biofungicide formulation N1E to control gray mold disease of plants, was investigated to study the bacterial traits that may be involved in its biological control activity. Two N1E based formulations, bacterial cell based formulation PN1E and culture supernatant based formulation SN1E, were evaluated for disease control activity against gray mold disease of tomato and strawberry plants. Neither PN1E nor SN1E was as effective as the original formulation N1E. Fractionation of antifungal compounds from the bacterial culture supernatant of B. licheniformis N1 indicated that two different cyclic lipopeptides were responsible for the antimicrobial activity of the N1 strain. These two purified compounds were identified as iturin A and surfactin by HPLC and LCMS. The purified lipopeptides were evaluated for plant disease control activity against seven plant diseases. Crude extracts and purified compounds applied at 500 ${\mu}g/ml$ concentration controlled tomato gray mold, tomato late blight and pepper anthracnose effectively with over 70% disease control value. While iturin showed broad spectrum activity against all tested plant diseases, the control activity by surfactin was limited to tomato gray mold, tomato late blight, and pepper anthracnose. Although antifungal compounds from B. licheniformis N1 exhibited disease control activity, our results suggested that bacterial cells present in the N1E formulation also contribute to the disease control activity together with the antifungal compounds.
Kong, Hyun-Gi,Choi, Ki-Hyuck,Heo, Kwang-Ryool,Lee, Kwang-Youll,Lee, Hyoung-Ju,Moon, Byung-Ju,Lee, Seon-Woo The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.2
Marking biocontrol bacteria is an essential step to monitor bacterial behavior in natural environments before application in agricultural ecosystem. In this study, we presented the simple green fluorescent protein (GFP) reporter system driven by the promoter active in Bacillus species for tagging of the biocontrol bacteria. A constitutive promoter P43 from Bacillus subtilis was fused to an enhanced promoterless gfp gene by overlap extension PCR. The GFP expression was demonstrated by the high fluorescence intensity detected in B. subtilis and Escherichia coli transformed with the P43-gfp fusion construct, respectively. The GFP reporter system was further investigated in two bacterial biocontrol strains B. licheniformis and Pseudomonas fluorescens. When the reconstructed plasmid pWH34G was introduced into B. licheniformis, GFP level measured with the fluorescence intensity in B. licheniformis was almost equivalent to that in B. subtilis. However, GFP expression level was extremely low in other biocontrol bacteria P. fluorescens by transposon based stable insertion of the P43-gfp construct into the bacterial chromosome. This study provides information regarding to the efficient biomarker P43-gfp fusion construct for bio-control Bacillus species.
Kim, Byung Gon,Shin, Weon Ho,Lim, Soo Yeon,Kong, Byung Seon,Choi, Jang Wook The Korean Electrochemical Society 2012 Journal of electrochemical science and technology Vol.3 No.3
As an effort to address the chronic capacity fading of Si anodes and thus achieve their robust cycling performance, herein, we develop a unique electrode in which silicon nanoparticles are embedded in the carbon nanotubes network. Utilizing robust contacts between silicon nanoparticles and carbon nanotubes, the composite electrodes exhibit excellent electrochemical performance : 95.5% capacity retention after 140 cycles as well as rate capability such that at the C-rate increase from 0.1C to 1C to 10C, the specific capacities of 850, 698, and 312 mAh/g are obtained, respectively. The present investigation suggests a useful design principle for silicon as well as other high capacity alloying electrodes that undergo large volume expansions during battery operations.
Fe-Cr-Al계 분말 다공체 금속과 박판재의 고온 산화 거동
임선희 ( Seon Hui Lim ),오재성 ( Jae Sung Oh ),공영민 ( Young Min Kong ),김병기 ( Byung Kee Kim ),박만호 ( Man Ho Park ),이기안 ( Kee Ahn Lee ) 대한금속재료학회(구 대한금속학회) 2013 대한금속·재료학회지 Vol.51 No.10
This study investigated the high temperature oxidation behaviors of Fe-Cr-Al powder porous metal (565 μm pore size) and a strip (as a reference of bulk material). A 24-hour TGA test was conducted at three different temperatures, 900 ℃, 1000 ℃, and 1100 ℃. High temperature oxidation results showed that the strip exhibited even higher levels of oxidation resistance than that of porous metal regardless of temperature. Oxidation weight gain gradually increased with increasing oxidation temperature. In the porous metal oxidized at 900 ℃ and 1000 ℃, the oxides Al2O3 and Cr2O3 were mainly observed. The porous metal oxidized at 1100 ℃ also revealed the presence of Fe-based oxides in a large quantity in addition to the oxides formed at lower temperature. The porous metal with a larger specific area represented increasingly faster dwindling of Al and Cr elements and easy progress to internal oxidation, compared to the oxidation of the bulk strip. (Received January 28, 2013)
GO-23 : Chemosensitivity testing based on gene expression profiling in patients with ovarian cancer
( Geum Seon Sohn ),( Hyeong Ju Kim ),( Ji Yun Lee ),( Jinkyoung Kong ),( Ji Hee Choi ),( Hanbyoul Cho ),( Eun Ji Nam ),( Sang Wun Kim ),( Sunghoon Kim ),( Jae Hoon Kim ),( Young Tae Kim ),( Doo Byung 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-
목적: To evaluate the association between clinical response of treatment agents and results of chemosensitivity testing in ovarian cancer. 방법: Tissue was obtained from 21 ovarian cancer patients and gene expression was evaluated by quantitative real-time polymerase chain reaction (PCR). Selected gene panel with expression of specific genes in the pathways that are related to drug responses in ovarian cancer were analyzed( AKT, Aurora A, BCRP, CD31, ERCC1, GSTpi, HER2, MDR1, Mitosin, PI3 Kinase, RRM1, Survivin, TOP1, TOP2A, TS, VEGF, VEGFR2, XIAP, P73). Gene expression were matched with therapeutic agent including Platinum, Taxanes, Bevacizumab, Gemcitabine, Topoisomerase I Inhibitors, Topoisomerase II Inhibitors, Cyclophosphamide, Herceptin, and 5-fluorouracil for chemosensitivity. 결과: Chemosensitivity testing revealed sensitivity rate of 66%, 81%, 96%, 56% and 61% for Platinum, Taxanes, Topoisomerase I Inhibitors, Topoisomerase II Inhibitors, and Bevacizumab, respectively. Treatment response rate was 70% (Complete Response: 40%, Partial Response: 30%). Treatment response was not significantly increased in the platinum sensitive patients (p=0.613), and overall response rate did not significantly differ according to the chemosensitivity test. 결론: This study may provide useful information in optimizing individual chemotherapy in the treatment of ovarian cancer.
강병주(Byung-Joo Kang),정우재(Woo-Jae Jung),이호선(Ho-Seon Lee),공동욱(Tong-Ook Kong),김재영(Jae-Young Kim),전정익(Jung-ik Chun),전종훈(Jong-Hoon Chun) 한국전자파학회 2020 한국전자파학회논문지 Vol.31 No.3
본 논문에서는 연직바람관측장비에 적용 가능한 64배열 능동위상배열안테나에 대한 연구 결과를 기술하였다. 주요 구성부인 위상배열안테나는 인접 안테나와 격리도를 개선한 Bended 다이폴 안테나의 형태이며, 시스템 파라미터 분석에 근거하여 64개의 단일 송수신 모듈을 설계하였다. 특히 단일 송수신 모듈은 위상 및 감쇄 제어 기능이 있어, 능동위상배열안테나의 핵심 역할을 한다. 이를 통해 개발된 64배열 능동위상배열안테나는 적합한 안테나 이득 및 빔폭 특성과 함께 전자적 제어에 의한 20° 빔 조향 각도에서 왜곡 없는 우수한 방사 특성을 확인하였다. 그러므로 본 논문에서 개발한 64배열 능동위상배열안테나는 연직바람관측장비 개발에 적용 가능함을 검증하였다. In this study, we demonstrate the results of a 64-array active phased antenna for radar wind profiler. The main part, the active phased array antenna, had the form of a bent dipole antenna with improved isolation from the adjacent antenna. Based on the system parameter analysis, 64 transmit/receive modules(TRMs) were designed. In particular, the TRMs have the function of phase/attenuation control, which plays a key role in the active phased array antenna. Measurement results of the 64-array active phased antenna, with a 20° beam steering angle with electronic control, showed the proper antenna gain, good beamwidth characteristics, and excellent radiation characteristics without distortion. These results indicate that the active phased array antenna can be applied to radar wind profilers.
Choi, Byung-Hyun,Mesalam, Ayman,Song, Seok-Hwan,Joo, Myeong-don,Hwang, Ji-Yoon,Oh, Seon-Hwa,Lee, Kyeong-Lim,Kong, Il-Keun The Korean Society of Embryo Transfer 2018 한국동물생명공학회지 Vol.33 No.4
Zona pellucida (ZP), a primarily representative coat of mammalian egg and embryo, has an extremely heterogeneous morphology during different developmental stages. The objective of the present study was to compare the morphological changes of the ZP surface of immature, in vitro and in vivo matured canine oocytes by using scanning electron microscopy (SEM). Canine ovaries were collected from local veterinary hospitals to recover immature oocytes. The ovaries were sliced and the released cumulus oocyte complexes (COCs) were washed with TL-HEPES. The selected COCs were randomly divided into two groups, first group was processed immediately at immature state and the second group was processed 72 h after in vitro maturation, and compared with in vivo derived oocytes. Oocytes were fixed, critical point dried and examined under SEM. The diameters of oocyte and outer holes of the ZP were measured on a total of 249 oocytes; the results were analyzed using One-way ANOVA. Our results showed that, the diameter of immature oocytes significantly differed (p < 0.05) from that of in vivo matured oocytes ($79.60{\pm}0.77{\mu}m$ vs. $101.46{\pm}1.07{\mu}m$, respectively). Similarly, a significant difference (p < 0.05) in the diameters between those of in vitro and in vivo matured oocytes were found ($79.51{\pm}2.36{\mu}m$ vs. $101.46{\pm}1.07{\mu}m$, respectively). Moreover, the diameters of the outer holes of the ZP were significantly (p < 0.05) larger in in vivo matured ($1.48{\pm}0.42{\mu}m$) than in vitro matured for 72 and immature oocytes ($1.10{\pm}0.16$ and $0.43{\pm}0.12{\mu}m$, respectively). Taken together, these data indicates that the ZP surface is related to oocyte maturity in canine.