Irrigation Regimes and Bio-stimulant Application Effects on Yield and Morpho-Physiological Responses of Strawberry

Burcak Kapur,Eser Celiktopuz,Mehmet Ali Sarıda?,Sevgi Payda? Kargı 한국원예학회 2018 원예과학기술지 Vol.36 No.3

In the Mediterranean region of Turkey, strawberry production is a vital part of the agricultural sector, providing high rates of employment and farm income. Optimizing water application and effective cultivation practices are of considerable importance in improving strawberry yield. In this study the response of strawberry a cultivar (Fragaria ananassa Duch. cv. Rubygem) to irrigation regimes and bio-stimulant (seaweed extract) use were investigated, by evaluating the yield and morpho-physiological parameters under high tunnel conditions in the Mediterranean environment. The amounts of irrigation water applied were 0.50, 0.75, 1.00 and 1.25 times the water surface evaporation, measured by a standard Class A pan, and the corresponding regimes were denoted as Ir50, Ir75, Ir100, and Ir125, respectively. There was a significant decrease in total berry yield and number of berries in the Ir50 irrigation regime. The maximum total berry yield was attained at Ir75 in both applications (585.7 g/plant and 521.9 g/plant under bio-stimulant and control applications, respectively). However, the results of Ir75, Ir100 and Ir125 did not reveal any significant yield benefit. The bio-stimulant application provided a significant, 17% increase, in berry yield. The stomatal conductance and leaf water potential were reduced as irrigation application rates decreased. Conversely, stomatal conductance and leaf water potential were statistically higher, 41 μmol · m<SUP>-2</SUP> · s<SUP>-1</SUP> and 0.5 bar, respectively, after the use of the bio-stimulant, as it alleviated the negative effects of water stress. Bio-stimulant application also significantly increased fruit nitrogen content by approximately 14%. In conclusion, considering the irrigation regimes of strawberries grown under high tunnels in the Mediterranean environment, water use is recommended at around 274 mm (Ir75), along with bio-stimulant application for optimal yield.

Investigating the Potential of Lipids for Use as Biomarkers for Glioblastoma via an Untargeted Lipidomics Approach

Burcak Soylemez,Zekeriya Bulut,Serap Şahin-Bölükbaşı 대한신경외과학회 2023 Journal of Korean neurosurgical society Vol.66 No.2

Objective : The types and functions of lipids involved in glioblastoma (GB) are not well known. Lipidomics is a new field that examines cellular lipids on a large scale and novel aplication of lipidomics in the biomedical sciences have emerged. This study aimed to investigate the potential of blood lipids for use as biomarkers for the diagnosis of GB via untargated lipidomic approach. Gaining a deeper understanding of lipid metabolism in patients with GB can contribute to the early diagnosis with GB patiens and also development of novel and better therapeutic options. Methods : This study was performed using blood samples collected from 14 patients (eight females and six males) and 14 controls (eight females and six males). Lipids were extracted from blood samples and quantified using phosphorus assay. Lipid profiles of between patients with GB and controls were compared via an untargeted lipidomics approach using 6530 Accurate-Mass Q-TOF LC/MS mass spectrometer. Results : According to the results obtained using the untargeted lipidomics approach, differentially regulated lipid species, including fatty acid (FA), glycerolipid (GL), glycerophospholipid (PG), saccharolipid (SL), sphingolipid (SP), and sterol lipid (ST) were identified between in patients with GB and controls. Conclusion : Differentially regulated lipids were identified in patients with GB, and these lipid species were predicted as potential biomarkers for diagnosis of GB.

Fabricating Tissues: Analysis of Farming Versus Engineering Strategies

Cheema Umber,Nazhat Showan N.,Alp Burcak,Foroughi Farhad,Anandagoda Nelomi,Mudera Vivek,Brown Robert A. Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.1

Tissue Engineering has expanded rapidly towards target applications of tissue repair and regeneration, whilst generating surprisingly novel models to study tissue modelling. However, clinical success in producing effective engineered tissues such as bone, skin, cartilage, and tendon, have been rare and limited. Problems tend to focus on how to stimulate the replacement of initial scaffold with mechanically functional, native extracellular matrix (principally collagen). Typical approaches have been to develop perfused and mechanically active bioreactors, with the use of native collagen itself as the initial scaffold, though the idea remains that cells do the fabrication (i.e. a cultivation process). We have developed a new, engineering approach, in which the final collagen template is fabricated without cell involvement. The first part of this biomimetic engineering involves a plastic compression of cellular native collagen gels to form dense, strong, collagenous neotissues (in minutes). Further steps can be used to orientate and increase collagen fibril diameter, again by non-cell dependent engineering. This allows operator control of cell or matrix density and material properties (influencing biological half life and fate). In addition, this (non-cultivation) approach can incorporate techniques to generate localised 3D structures and zones at a meso-scale. In conclusion, the use of biomimetic engineering based on native collagen, rather than cell-cultivation approaches for bulk matrix fabrication, produces huge benefits. These include speed of fabrication (minutes instead of weeks and months), possibility of fine control of composition and 3D nano-micro scale structure and biomimetic complexity.

Fabricating Tissues: Analysis of Farming versus Engineering Strategies

Robert A. Brown,Umber Cheema,Showan N. Nazhat,Burcak Alp,Farhad Foroughi,Nelomi Anandagoda,Vivek Mudera 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.1

Tissue Engineering has expanded rapidly towards target applications of tissue repair and regeneration, whilst generating surprisingly novel models to study tissue modelling. However, clinical success in producing effective engineered tissues such as bone, skin, cartilage, and tendon, have been rare and limited. Problems tend to focus on how to stimulate the replacement of initial scaffold with mechanically functional, native extracellular matrix (principally collagen). Typical approaches have been to develop perfused and mechanically active bioreactors, with the use of native collagen itself as the initial scaffold, though the idea remains that cells do the fabrication (i.e. a cultivation process). We have developed a new, engineering approach, in which the final collagen template is fabricated without cell involvement. The first part of this biomimetic engineering involves a plastic compression of cellular native collagen gels to form dense, strong, collagenous neo-tissues (in minutes). Further steps can be used to orientate and increase collagen fibril diameter, again by non-cell dependent engineering. This allows operator control of cell or matrix density and material properties (influencing biological half life and fate). In addition, this (non-cultivation) approach can incorporate techniques to generate localised 3D structures and zones at a meso-scale. In conclusion, the use of biomimetic engineering based on native collagen, rather than cell-cultivation approaches for bulk matrix fabrication, produces huge benefits. These include speed of fabrication (minutes instead of weeks and months), possibility of fine control of composition and 3D nano-micro scale structure and biomimetic complexity.

Iron Supplementation in Experimental Hyperthyroidism: Effects on Oxidative Stress in Skeletal Muscle Tissue

Hakki Oktay Seymen,Sabiha Civelek,Arzu Seven,Gunnur Yigit,Husrev Hatemi,Gulden Burcak 연세대학교의과대학 2004 Yonsei medical journal Vol.45 No.SUP

This study was designed to investigate the effects of iron supplementation on the parameters of oxidative stress in the skeletal muscle tissue of hyperthyroidism induced rats. Hyperthyroidism was found to cause an increase in thiobarbituric acid-reactive substances (TBARS) and copper zinc superoxide dismutase (Cu, Zn SOD) activity, but decreases in the glutathione-peroxidase (GSH Px) activity and glutathione (GSH). Iron supplementation caused an increase in TBARS and a decrease in GSH. Iron supplementation in hyperthyroid rats attenuated the hyperthyroid state, but lowered the plasma ferritin level, which is considered an indicator of thyroid hormone action. Iron supplementation caused no additional increase in the TBARS in hyperthyroid rats, ameliorated the decrease in GSH content and abolished the induction of Cu, Zn SOD. Our findings suggested no increase, but a decrease, in the risk of oxidative stress in iron supplemented hyperthyroid rats. Whether supplementation of iron would have similar effects in humans should be further investigated in clinical studies.

Effects of Vitamin E Supplementation on Oxidative Stress in Streptozotocin Induced Diabetic Rats: Investigation of Liver and Plasma

Arzu Seven,Sava Guzel,Oktay Seymen,Sabiha Civelek,Murat Bolayirli,Murat Uncu,Gulden Burcak 연세대학교의과대학 2004 Yonsei medical journal Vol.45 No.4

This experimental study was designed to investigate the effects of vitamin E supplementation, especially on lipid peroxidation and antioxidant status elements¾ namely, glutathione (GSH), CuZn superoxide dismutase (CuZn SOD), and glutathione peroxidase (GSH Px), both in blood and liver tissues of streptozotocin (STZ) diabetic rats. The extent to which blood can be used to reflect the oxidative stress of the liver is also investigated. In diabetic rats, plasma lipid peroxide values were not significantly different,from control,whereas erythrocyte CuZn SOD (p<0.01), GSH Px (p<0.001) activities and plasma vitamin E levels (p<0.001), were significantly more elevated than controls. Vitamin E supplementation caused significant decreases of erythrocyte GSH level (p<0.01) in control rats and of erythrocyte GSH Px activity (p<0.05) in diabetic rats. Liver findings revealed significantly higher lipid peroxide (p<0.001) and vitamin E (p<0.01) levels and lower GSH (p<0.001), CuZn SOD (p<0.001) and GSH Px (p<0.01) levels in diabetic rats. A decreased hepatic lipid peroxide level (p<0.01) and increased vitamin E/lipid peroxide ratio (p<0.001) were observed in vitamin E supplemented, diabetic rats. A vitamin E supplementation level which did not cause any increase in the concentration of the vitamin in the liver or blood, was sufficient to lower lipid peroxidation in the liver. Vitamin E/lipid peroxide ratio is suggested as an appropriate index to evaluate the efficiency of vitamin E activity,independent of tissue lipid values. Further, the antioxidant components GSH, GSH Px and CuZn SOD and the relationships among them, were affected differently in the liver and blood by diabetes or vitamin E supplementation.