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Brito-Zeró,n, Pilar,Acar-Denizli, Nihan,Zeher, Margit,Rasmussen, Astrid,Seror, Raphaele,Theander, Elke,Li, Xiaomei,Baldini, Chiara,Gottenberg, Jacques-Eric,Danda, Debashish,Quartuccio, Luca,Prio BMJ Publishing Group Ltd 2017 Annals of the Rheumatic Diseases Vol.76 No.6
<P>Conclusions This study provides the first evidence of a strong influence of geolocation and ethnicity on the phenotype of primary SjS at diagnosis.</P>
Brito Giovani G.,Concenço Germani,Costa Vladimir E.,Fagundes Paulo Ricardo R.,da Silva-Filho João Luis,Parfitt José Maria B.,Magalhães Ariano,Silva Giovana T.,Jardim Thaís M.,Luccas Nathália Furtado,S 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.1
Phenotyping is the major bottleneck in the efort to develop varieties of rice (Oryza sativa L.) suitable for growing under a water-saving irrigation management, such as alternate wetting and drying irrigation techniques (AWD). To analyze if the genotypic variability for carbon isotope discrimination (CID) in rice leaves could be used as a relatively high-throughput tracer to early select superior genotypes highlighting improved root architecture traits when submitted to AWD, a set of twenty varieties grown under semi-natural conditions were submitted to two water irrigation regimes, continuous fooding (CF) and AWD cycles. Coefcients of genetic variance (π2g) obtained for root architecture, micro-morphological and physiological traits were signifcant for all of them regardless of the adopted irrigation system, except to mean root diameter. The three signifcant principal components (PCs) with eigenvalue>1, explain most of the total variation across cycles and water regimes. For most of analyzed traits, the values of heritability coefcients were higher regardless of adopted irrigation management and trait category; for CID, the magnitudes of broad heritability at an individual level (greater than 0.80) were similar in the two irrigation techniques, evidencing that the success of the selection is independent of irrigation management. The higher CID values after three AWD cycles are associated with varieties with higher total root length and volume. To our knowledge, this is the frst study demonstrating the potential application of CID as a tracer to select root architecture traits in rice when water-saving irrigation management is of concern
Brito-Carreón Cesar Armando,Zavala-Maldonado Karla,Suárez-Andino Erika Ivette,David Randy E.,Perez- Ramírez Gerardo,Diaz-Badillo Alvaro,Muñoz María de Lourdes 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2
Currently, there are no antiviral drugs that effectively reduce the risks and treat the symptoms associated with dengue virus (DENV). Consequently, efforts remain primarily focused on transmission reduction. One such effort concerns DENV receptors in mosquito vectors Aedes aegypti and Aedes albopictus. Despite a lack of direct evidence demonstrating the binding of DENV to cells in mosquito vectors, one putative DENV binding protein has been α-enolase. To develop a deeper understanding, this study tested whether DENV proteins bind to enolase localized in the cytoplasmic membrane of C6/36 cells using both anti-enolase-specific antibodies, and by colocalization analysis, using confocal microscopy. Additionally, to probe the interaction of enolase with the DENV E protein, we performed a docking analysis using PatchDock and FireDock software packages. Study results demonstrate that the DENV E protein interacts with enolase in the plasma membrane of C6/36 cells of Ae. albopictus. Specific anti-enolase antibodies were found to inhibit DENV infection of these cells. Moreover, enolase was found to be localized to the cytoplasmic mem brane, cytoplasm, and nucleus. These combined findings suggest that enolase participates in the entrance mechanism of DENV into vector cells.
GPU-enabled back-propagation artificial neural network for digit recognition in parallel
Brito, R.,Fong, S.,Cho, K.,Song, W.,Wong, R.,Mohammed, S.,Fiaidhi, J. Springer Science + Business Media 2016 The Journal of supercomputing Vol.72 No.10
<P>In this paper, we show that the GPU (graphics processing unit) can be used not only for processing graphics, but also for high speed computing. We provide a comparison between the times taken on the CPU and GPU to perform the training and testing of a back-propagation artificial neural network. We implemented two neural networks for recognizing handwritten digits; one consists of serial code executed on the CPU, while the other is a GPU-based version of the same system which executes in parallel. As an experiment for performance evaluation, a system for neural network training on the GPU is developed to reduce training time. The programming environment that the system is based on is CUDA which stands for compute unified device architecture, which allows a programmer to write code that will run on an NVIDIA GPU card. Our results over an experiment of digital image recognition using neural network confirm the speed-up advantages by tapping on the resources of GPU. Our proposed model has an advantage of simplicity, while it shows on par performance with the state-of-the-arts algorithms.</P>
Zinc and Its Transporters in Epigenetics
Brito, Sofia,Lee, Mi-Gi,Bin, Bum-Ho,Lee, Jong-Soo Korean Society for Molecular and Cellular Biology 2020 Molecules and cells Vol.43 No.4
Epigenetic events like DNA methylation and histone modification can alter heritable phenotypes. Zinc is required for the activity of various epigenetic enzymes, such as DNA methyltransferases (DNMTs), histone acetyltransferases (HATs), histone deacetylases (HDACs), and histone demethylases, which possess several zinc binding sites. Thus, the dysregulation of zinc homeostasis can lead to epigenetic alterations. Zinc homeostasis is regulated by Zinc Transporters (ZnTs), Zrt- and Irt-like proteins (ZIPs), and the zinc storage protein metallothionein (MT). Recent advances revealed that ZIPs modulate epigenetics. ZIP10 deficiency was found to result in reduced HATs, confirming its involvement in histone acetylation for rigid skin barrier formation. ZIP13 deficiency, which is associated with Spondylocheirodysplastic Ehlers-Danlos syndrome (SCD-EDS), increases DNMT activity, leading to dysgenesis of dermis via improper gene expressions. However, the precise molecular mechanisms remain to be elucidated. Future molecular studies investigating the involvement of zinc and its transporters in epigenetics are warranted.
( Pilar Brito Zeron ),( Hoda Gheitasi ),( Marta Perez De Lis Novo ),( Noelia Armiger ),( Maria Jose Soto Cardenas ),( Myriam Gandia ),( Albert Bove ),( Xavier Bosch ),( Soledad Retamozo ),( Miriam Aka 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: To investigate the mechanisms involved in lymphocyte activation mediated by the CD5 immunoreceptor in patients with systemic autoimmune diseases related to B-cell hyperactivity. Methods: A total of 508 patients were analyzed (273 primary Sjogren syndrome patients, 135 SLE patients, 47 SS associated HCV patients and 53 controls). CD5 genotyping receptor was analyzed by PCR-SBT technique with the detection of the SNP7 (rs2241002) andSNP26 (rs2229177). The homozygous haplotype CC/CC was classifi ed as inductor of autoimmunity, whereas the heterozygous haplotype CC/TT was a protective factor. Results: The haplotype CC/CC was found in 7.5% of controls vs 19% of patients with primary SS fulfi lling the 2002 criteria (p=0.047), 12% of SS-1993 criteria (p=0.39), 18% of SLE (p=0.10) and 21% of SS-HCV patients (p=0.049); the protective haplotype CC/TT was observed in 28% of controls vs 19% of patients with primary SS-2002 criteria (p=0.15), 13% of SS-1993 criteria (p=0.01), 15% of SLE (p=0.04) and 15% of SS-HCV patients (p=0.10). A specifi c analysis was carried out in patients with primary SS-2002 criteria comparing the clinical and immunological features of patients carrying protective haplotypes (n=33) and those carrying inductor haplotypes (n=33). A higher frequency of anti-La/SS-B antibodies (67% vs 39%, p=0.048) and a trend for an increased frequency of extraglandular involvement (52% vs 36%), neutropenia (33% vs 18%), ANA (91% vs 82%), FR (53% vs 48%) and low levels of C3 (12% vs 3%) was found in carriers of inductor haplotypes with respect to those carrying protective haplotypes. Conclusions: We found a higher frequency of the CC/CC haplotype (associated with increased autoimmunity) of the gene that encodes the immunoreceptor lymphocytic CD5 in SS patients, that was related to an increased extraglandular systemic activity, especially a higher frequency of anti-La/SS-B antibodies.
Serum Albumin Modulates the Bioactivity of Rosmarinic Acid
Elsa Brito,Andre Silva,Pedro Luis Vieira Fale,Rita Pacheco,Antonio Serralheiro,Parvez I. Haris,Lia Ascensao,Maria Luısa Serralheiro 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.8
Rosmarinic acid (RA) is a phenolic compound with biological activity. The objective of the present study was to investigate whether this compound kept its biological activity in the presence of proteins. For this purpose, bovine serum albumin (BSA) was used as a model protein, and the capacity of the RA to inhibit acetylcholinesterase (AChE) and affect antioxidant activity was evaluated in the absence and presence of BSA. A mixture of phenolic compounds containing RA, obtained from a medicinal plant was added to this study. The AChE inhibitory activity of RA was reduced by ∼57% in the presence of BSA, while the antioxidant activity increased. These results lead to the investigation of the effect of RA on the BSA structure using Fourier transform infrared spectroscopy (FTIR). At 37°C and higher temperatures, RA caused a decrease in the temperature modifications on the protein structure. Furthermore, FTIR and native-gel analysis revealed that protein aggregation/precipitation, induced by temperature, was reduced in the presence of RA. The novelty of the present work resides in the study of the enzyme inhibitory activity and antioxidant capacity of polyphenols, such as RA, in the presence of a protein. The findings highlight the need to consider the presence of proteins when assessing biological activities of polyphenols in vitro and that enzyme inhibitory activity may be decreased, while the antioxidant capacity remains or even increases.
( Pilar Brito Zeron ),( Marta Perez De Lis Novo ),( Pilar Rosich ),( Carles Tolosa ),( Joaquim Oristrell ),( Luis Saez Comet ),( Mercedes Perez Conesa ),( Jose Antonio Vargas Hitos ),( Jose Mario Sabi 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: To analyze the outcomes and therapeutic management of affected pregnancies with autoimmune congenital heart block (aCHB). Methods: Retrospective multicenter study in Internal Medicine Departments; inclusion criteria consisted of: aCHB of any type (I, II or III), fetal EFE and/or cardiomyopathy, cardiac block diagnosed in utero or in the first postpartum month, and mothers carrying anti-Ro52, Ro60 and/or La autoantibodies. Results: A total of 25 pregnancies with aCHB were retrospectively analyzed in 21 anti-Ro/La+ mothers. The mean maternal age at the time of pregnancy with aCHB was 33,25 years. Only 2 mothers received treatment prior to the first affected pregnancy (hydroxychloroquine and hydroxychloroquine+prednisone). Cardiac block consisted of type I (n=1), type II (n=6) and type III (n=18). At diagnosis of aCHB, 15/22 women were treated with dexamethasone (one of them, along with IVIG) and 2/22 with ritodrine. Preventive treatment with IVIG was administered in 2 pregnancies in which a recurrence was observed. Pregnancy was interrupted in 7/25 pregnancies at a mean week of 23.43 (18.2-37), while 18/25 of pregnancies gave a live birth baby with a mean age of 35.71 weeks of birth (30-40). 11 babies required pacemaker implantation, 9 immediately after birth and 2 in the neonatal period (1 at 5 years of age and another at 12). Of the 15 pregnancies with aCHB treated with dexamethasone, 12 achieved pregnancy to term (1 type II disappeared, with no changes in the remaining cases) and there were 3 fetal deaths despite treatment. Of the 7 pregnancies not treated with dexamethasone, 3 babies were born alive (no reversal of the blockade) and there were 4 fetal deaths. Conclusions: aCHB is a serious problem with a fetal mortality of 28% and a high requirement for neonatal pacemaker placement (61%).
Panoramic radiographs underestimate extensions of the anterior loop and mandibular incisive canal
de Brito, Ana Caroline Ramos,Nejaim, Yuri,de Freitas, Deborah Queiroz,Santos, Christiano de Oliveira Korean Academy of Oral and Maxillofacial Radiology 2016 Imaging Science in Dentistry Vol.46 No.3
Purpose: The purpose of this study was to detect the anterior loop of the mental nerve and the mandibular incisive canal in panoramic radiographs (PAN) and cone-beam computed tomography (CBCT) images, as well as to determine the anterior/mesial extension of these structures in panoramic and cross-sectional reconstructions using PAN and CBCT images. Materials and Methods: Images (both PAN and CBCT) from 90 patients were evaluated by 2 independent observers. Detection of the anterior loop and the incisive canal were compared between PAN and CBCT. The anterior/mesial extension of these structures was compared between PAN and both cross-sectional and panoramic CBCT reconstructions. Results: In CBCT, the anterior loop and the incisive canal were observed in 7.7% and 24.4% of the hemimandibles, respectively. In PAN, the anterior loop and the incisive canal were detected in 15% and 5.5% of cases, respectively. PAN presented more difficulties in the visualization of structures. The anterior/mesial extensions ranged from 0.0 mm to 19.0 mm on CBCT. PAN underestimated the measurements by approximately 2.0 mm. Conclusion: CBCT appears to be a more reliable imaging modality than PAN for preoperative workups of the anterior mandible. Individual variations in the anterior/mesial extensions of the anterior loop of the mental nerve and the mandibular incisive canal mean that is not prudent to rely on a general safe zone for implant placement or bone surgery in the interforaminal region.