The in vitro and in vivo efficacy of fluconazole in combination with farnesol against Candida albicans isolates using a murine vulvovaginitis model

Aliz Bozó,Marianna Domán,László Majoros,Gábor Kardos,István Varga,Renátó Kovács 한국미생물학회 2016 The journal of microbiology Vol.54 No.11

Farnesol is a quorum-sensing molecule that inhibits biofilm formation in Candida albicans. Previous in vitro data suggest that, in combination with certain antifungals, farnesol may have an adjuvant anti-biofilm agent. However, the in vivo efficacy of farnesol is very questionable. Therefore, the in vitro and in vivo activity of fluconazole combined with farnesol was evaluated against C. albicans biofilms using fractional inhibitory concentration index (FICI) determination, time-kill experiments and a murine vulvovaginitis model. The median biofilm MICs of fluconazole-sensitive C. albicans isolates ranged between 4 -> 512 mg/L and 150–300 μM for fluconazole and farnesol, respectively. These values were 512 -> 512 mg/L and > 300 μM for fluconazole-resistant clinical isolates. Farnesol decreased the median MICs of fluconazole by 2-64-fold for biofilms. Based on FICI, synergistic interaction was observed only in the case of the sessile SC5314 reference strain (FICIs: 0.16–0.27). In time-kill studies, only the 512 mg/L fluconazole and 512 mg/L fluconazole + 75 μM farnesol reduced biofilm mass significantly at each time point in the case of all isolates. The combination reduced the metabolic activity of biofilms for all isolates in a concentration- and time-dependent manner. Our findings revealed that farnesol alone was not protective in a murine vulvovaginitis model. Farnesol was not beneficial in combination with fluconazole for fluconazole-susceptible isolates, but partially increased fluconazole activity against one fluconazole- resistant isolate, but not the other one.

Sustainable production of acrolein over highly stable and selective WO3 over SiO2-TiO2 catalysts

Boz Ismail,Boroglu Mehtap Safak,Zengin Yasar,Kaya Busra 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.8

The effects of the addition of colloidal silica (cSiO2) and solvents used in the catalyst preparation on the activity and stability of WO3-TiO2 catalysts are reported in this paper. The highly stable and selective WO3 supported cSiO2-TiO2 catalysts were prepared and tested in the vapor-phase glycerol oxy-dehydration. WO3-TiO2 catalysts with and without cSiO2 were characterized by XRD, SEM, NH3-TPD, infrared spectroscopy of pyridine (FTIR-Py), XPS, RAMAN, and N2 adsorption-desorption (BET). The highest medium strength acidity and optimum Brønsted to Lewis acid site ratio of WO3 catalysts were achieved upon the addition of colloidal silica (cSiO2) onto TiO2 support. The medium strength acidity of Brønsted acid sites was responsible for the improved acrolein selectivity and stability. The other major factors in glycerol conversion and acrolein selectivity were the glycerol content and liquid hourly space velocity. The yield to acrolein was up to 70% and kept almost constant in a 50 h continuous run at 300 °C. The gradual decrease in glycerol conversion was due to the build-up of oxygen-containing carbonaceous materials deposited on the catalyst surface.

The effect of process parameters on copper powder particle size and shape produced by electrolysis method

Mustafa Boz,Masood Hasheminiasari 국제구조공학회 2013 Steel and Composite Structures, An International J Vol.15 No.2

In this study, an electrolyzing device for the production of metal powders was designed and fabricated. The production of copper powders was performed using a variety of current densities, anode-cathode distances and power removal times. The effect of these parameters on powder particle size and shape was determined. Particle size was measured using a laser diffraction unit while the powder shape was determined by SEM. Experimental results show that an increase in current density leads to a decrease in powder particle size. In addition particle shape changed from globular dendritic to acicular dendritic with increasing the current density. Distance between the cathode and anode also showed a similar influence on powder particle size and shape. An increase in time of powder removal led to an increase in powder particle size, as the shape changed from acicular dendritic to globular dendritic.

Effect of different oils and sugar syrups on the properties of tray kadayif (traditional Turkish dessert)

Başar Burak,Boz Hüseyin 한국식품연구원 2023 Journal of Ethnic Foods Vol.10 No.13

The aim of this study was to analyze the effect of different oils and sugar syrups on the sensory and textural properties of traditional Turkish dessert the tray kadayif. The textural parameters of the tray kadayif samples were significantly (P < 0.01) affected by the replacement of sunflower oil and hazelnut oil with butter. The cutting hardness values of the tray kadayif samples increased with the increase in the amount of sunflower oil added to the formulations. The highest hardness values were achieved in formulations without butter in terms of both cutting hardness and fork hardness. The stickiness values reached the highest level in formulations containing 50% sunflower oil, 50% butter and 0% hazelnut oil. When the sugar syrups were compared, the stickiness values of the samples with sucrose syrup were found to be higher than the samples with glucose syrup. As a result of the sensory analysis conducted, though all the formulations were accepted by panelists, the highest score from the panelists was received by the formulations containing 75% butter and 25% oil (sunflower and hazelnut oil). It was also determined that the tray kadayif samples prepared with sucrose syrup had higher scores than the samples prepared with glucose syrup in terms of sensory properties.

Poster Session : PS 0123;Cardiology : Evaluation of the Microalbuminuria in Acute Coronary Syndrome Patients

( Suleyman Ahbab ),( Mustafa Boz ),( Ali Atay ),( Esra Atakoglu ),( Faik Oetin ),( Mustafa Yenigun ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Atherosclerosis and endothelial dysfunction are main pathological event for developing ischemic heart disease. It is damaged that the endothelial produced vasoactive and antithrombotic agents in the process of atherosclerosis and endothelial dysfunction occurs. Microalbuminuria is an indicator for the endothelial dysfunction. In this study, it was investigated the relationship between microalbuminuria and acute coronary syndrome. Methods: This study was consisted up 35 acute coronary syndrome patients without diabetes mellitus. Metabolic parameters and microalbuminuria in 24 hours urinary collection of the patients were analyzed. Above 30 mg/dl daily urinary albumin excretion was described as microalbuminuria (30-300 mg/dl). Microalbuminuric patients were followed up for one year. Patients parameters` mean and standart deviation were calculated. It was statiscically meaningful, p<0,05. Results: 27 male and 8 female, total 35 patients were added in the study group. It was determinated that the mortality and reinfarctus were higher in microalbuminuric patient. 3 reinfarctus and 3 mortality were determinated in normoalbuminuric patients. Fasting triglycerid and homocystein levels were lower in microalbuminuric patients. Conclusions: It was demonstrated that the prognostic signifi cance of microalbuminuria in the acute coronary syndrome patients for one year follow up.

Poster Session : PS 0160 ; Diabetes : Qt Dispersion: Comparison Between Type 2 Diabetic with Microalbuminuria Positive and Negative Patients

( Esma Altunoglu ),( Mustafa Boz ),( Cuneyt Muderrisoglu ),( Fusun Erdenen ),( Ender Ulgen ),( Mustafa Duran ),( Nurcan Ozbas ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: It is shown that the variability of distance between QT derivations in twelve derivation ECG; is risk factor for sudden death as a predictor of cardiovascular mortality in both diabetic and general population. Diabetic nephropathy is one of the most common complication of diabetes. The purpose was to investigate the relationship between QT parameters and microalbumin/creatinine ratio; as an indicator of endothelial function and a cardiovascular risk factor. Methods: 100 patients were included our study who were follow up Istanbul Education and Research Hospital Diabetes Outpatient Clinic. 50 patients whose microalbumin/ creatinine ratio less than 30 mg/dl and ages between 36 and 77 years were chosen as control group; 50 patients whose microalbumin/ creatinine ratio between 30- 300 mg/dl and ages between 36 and 79 years were chosen as patient group. The QT interval was measured on the 12-lead resting ECG. QTd was calculated automatically using special software. Results: In patients group QTC min median value was higher; 380,63(361,44-397,30), (p=0,042),QTC max median value was significantly higher; 439,63(420,17-460,22), (p=0,005) and microalbumin/creatinine value 82(60-300), (p=0,0001) was very signifi cantly higher than control group. There was no signifi cant difference QTC dispersion median value 61,25(49,91-81,70, (p=0,066) in both groups. Also a good correlation in positive direction between QTC dispersion values and microalbumin/creatinine value was determined. Conclusions: We researched the relation between microalbumin/creatinine ratio and QTd levels in type 2 diabetic patients. QTc max period was determined signifi cantly high in the group with microalbumin/creatinine between 30-300 mg/dl (p<0,05). As a result of this correlation QT prolongation can be use as a contributory parameter for prediction of cardiovascular risk. This study make us that QTd can be used as early indicator of cardiovascular risk in diabetic patients who aren`t in microalbuminuric stage yet.

The Effect of Problem Posing Oriented Calculus-2 Instruction on Academic Success

Hayri Akay,Nihat Boz 한국수학교육학회 2009 수학교육연구 Vol.13 No.2

Enhanced copper(II) biosorption on SiO2-alginate gel composite: A mechanistic study with surface characterization

Sibel Yalçın,Reşat Apak,İsmail Boz 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.10

A new SiO2-alginate biocomposite was synthesized with improved mechanical properties, showing chelation capability of divalent metal ions, especially Cu(II), through its carboxylic acid ends. The biocomposite was characterized using scanning electron microscopy (SEM), Fourier-transform infrared (FTIR), and X-ray photoelectron spectroscopy (XPS) techniques. The adsorption of copper(II) onto both H- and Ca-forms of sorbent was investigated as a function of pH and contact time, and adsorption data were modeled with the aid of Langmuir and Freundlich isotherms. The release of Ca(II) ions accompanying copper(II) binding was evaluated by selective surface complexation concept involving ion-exchange. The IR spectra gave detailed information on complexation of carbonyl group with copper ions and on the relative contribution of SiO2 involved in copper uptake. The adsorption edge of copper was within the pH range 4.0-5.5, and the sorbent capacity was determined as 1.85 and 1.10mmolg−1 for H- and Ca-forms, respectively.

The role of C-reactive protein ratio in predicting mortality in patients with Fournier gangrene

Eray Ismail Cem,Dalci Kubilay,Gumus Serdar,Yalav Orcun,Saritas Ahmet Gokhan,Boz Asli,Rencuzogullari Ahmet 대한대장항문학회 2023 Annals of Coloproctolgy Vol.39 No.3

Purpose: This study aimed to determine the C-reactive protein (CRP) ratio for the survival of patients with Fournier gangrene (FG). Methods: Fifty-two patients with FG between January 2011 and September 2018 were retrospectively analyzed. Data on clinical presentation, Fournier Gangrene Severity Index (FGSI), CRP ratio, management, and outcome were analyzed. The CRP ratio was calculated as preoperative CRP/postoperative CRP value that measured 48 hours after surgical intervention. Possible alternative cutoff points for the FGSI and CRP were determined by receiver operating characteristic (ROC) analyses. The risk factors related to the prognosis were evaluated by univariate and multivariable logistic regression analyses. Results: The mean CRP ratios were 6.7±6.6 in the survivor group and 1.2±0.8 in the nonsurvivor group (P=0.001). FGSI was significantly higher in the non-survivor group compared to survivor group (8.5±2.5 vs. 3.5±2.2, P=0.001). There was a negative correlation between FGSI and CRP ratio (r=–0.51). ROC analysis determined the cutoff value as 1.78 for CRP (sensitivity, 86%; specificity, 82%; area under the ROC curve, 0.90) to predict death. The incidence of death for patients with CRP ratio of ≤1.78 increased 26.7 fold for those with CRP ratio of >1.78 (95% confidence interval [CI], 4.8–146.5; P=0.001). In the multivariable logistic regression model, CRP ratio (odds ratio [OR], 10.3; 95% CI, 1.5–72.2; P=0.019) and FGSI (OR, 17.8; 95% CI, 2.6–121.1; P=0.003) were independent risk factors for death. Conclusion: The CRP ratio is a simple method to use to predict mortality in FG.

Poster Session : PS 0154 ; Diabetes : Do Diabetic Patients Use Their Glucometers Effectively: Awareness of the Importance of Self-Monitoring in Diabetes

( Feray Akbas ),( Hanife Usta Atmaca ),( Ilker Nihat Okten ),( Eda Nuhoglu ),( Mustafa Boz ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Home glucose monitoring is vital for diabetic patients to maintain acceptable glucose levels. We aimed to demonstrate how effective home glucose monitoring was among diabetic patients who were followed in internal medicine outpatient clinics of our hospital. Methods: Randomly selected 89 diabetic patients who were admitted to internal medicine outpatient clinic in a week and who had glucometers were included in the study and a questionnaire was administered. The answers were categorized and the results were evaluated with SPSS. Results: The questions and the percentage of answers are shown in Table 1. Although diabetes duration of the patients was mostly more than 5 years, only half of them had a glucometer for that period. Most of the patients didn`t calibrate their devices. Most strikingly, only 25% of the patients in general were given a proper education for the usage of glucometers. 72% of patients who claimed to have been educated were mostly given instructions by a pharmacist that cannot be considered as education and only 35% of this group were educated by a diabetes nurse or a doctor. Only 40% of the patients monitored their glucose levels regularly, rest of the patients used their glucometers rarely or none. Conclusions: Home monitoring of glucose levels for diabetic patients would approve blood glucose levels by making necessary treatment adjustments available. Providing the optimal treatment would prevent/delay diabetes related complications, improve quality of life and contribute to decrease health costs. Thus; it`s necessary to educate and follow our diabetic patients better and assure that they use their glucometers more effectively and frequently. To include the patients to the treatment and to monitoring of diabetes seems to be the only way to achieve a better outcome of the disease.