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Montazeri, Hamed,Bouzari, Saeid,Azadmanesh, Kayhan,Ostad, Seyed Nasser,Ghahremani, Mohammad Hossein Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.17
Cyclin E, a key coordinator of the G1 to S transition in the cell cycle, may be deregulated in several malignancies, including breast cancer. The most significant aberration in cyclin E is its elastase mediated proteolytic cleavage into tumor specific low molecular weight isoforms (LMW-Es). LMW-Es are biochemically hyperactive and biologically drive tumorigenesis in transgenic mouse models. Additionally, expression of LMW-Es has been correlated with poor survival in breast cancer cases. Here we determine whether expression of LMW-Es in a breast cancer cell line that is naturally devoid of these deregulated forms would alter their progression through each phase of the cell cycle. The results revealed that LMW-Es expression resulted in an increased doubling time, concomitant with a predominant increase in the population in the S phase of the cell cycle. Moreover, downregulation of p53 in LMW-Es cells resulted in additional shortening of the doubling time and enrichment of cells in the S and G2/M phases of the cell cycle. Furthermore, expression of LMW-Es sensitized cells to ${\beta}$-estradiol (E2) mediated growth and changed expression patterns of estrogen receptor and Bcl-2. Intriguingly, expression of LMW-Es could surpass anti-apoptotic effects raised by p53 upregulation. Taken together these studies suggest that overexpression of LMW-Es in collaboration with p53 loss results in altered g rowth properties of MCF-7 cells, enhancing the oncogenic activity of these ER positive breast cancer cells.
Jafari Erfaneh,Mostaan Saeid,Bouzari Saeid 질병관리본부 2020 Osong Public Health and Research Persptectives Vol.11 No.5
Objectives Infectious diarrhea is one of the most common causes of pediatric death worldwide and enteropathogenic Escherichia coli (EPEC) is one of the main causes. There are 2 subgroups of EPEC, typical and atypical, based on the presence or absence of bundle forming pili (bfp), of which atypical EPEC is considered less virulent, but not less pathogenic. Antimicrobial resistance towards atypical EPEC among children is growing and is considered a major problem. In this study the pattern of antibiotic resistance in clinical isolates was determined. Methods Using 130 isolates, antibiotic resistance patterns and phenotypes were assessed, and genotypic profiles of extended spectrum β-lactamase (ESBL) production using disc diffusion and PCR was carried out. Phylogenetic groups were analyzed using quadruplex PCR. Results There were 65 E. coli isolates identified as atypical EPEC by PCR, among which the highest antibiotic resistance was towards ampicillin, followed by trimethoprim-sulfamethoxazole, and tetracycline. Multidrug resistance was detected in 44.6% of atypical EPEC isolates. Around 33% of isolates were determined to be extended spectrum β-lactamase producers, and in 90% of isolates, genes responsible for ESBL production could be detected. Moreover, the majority of atypical EPEC strains belonged to Group E, followed by Groups B1, B2 and C. Conclusion High rates of multidrug resistance and ESBL production among atypical EPEC isolates warrant periodical surveillance studies to select effective antibiotic treatment for patients. It is considered a critical step to manage antibiotic resistance by avoiding unnecessary prescriptions for antibiotics.
Javadi, Maryam,Oloomi, Mana,Bouzari, Saeid Korea Genome Organization 2017 Genomics & informatics Vol.15 No.2
In this study, cytolethal distending toxin (CDT) producer isolates genome were compared with genome of pathogenic and commensal Escherichia coli strains. Conserved genomic signatures among different types of CDT producer E. coli strains were assessed. It was shown that they could be used as biomarkers for research purposes and clinical diagnosis by polymerase chain reaction, or in vaccine development. cdt genes and several other genetic biomarkers were identified as signature sequences in CDT producer strains. The identified signatures include several individual phage proteins (holins, nucleases, and terminases, and transferases) and multiple members of different protein families (the lambda family, phage-integrase family, phage-tail tape protein family, putative membrane proteins, regulatory proteins, restriction-modification system proteins, tail fiber-assembly proteins, base plate-assembly proteins, and other prophage tail-related proteins). In this study, a sporadic phylogenic pattern was demonstrated in the CDT-producing strains. In conclusion, conserved signature proteins in a wide range of pathogenic bacterial strains can potentially be used in modern vaccine-design strategies.
In silico analysis of Brucella abortus Omp2b and in vitro expression of SOmp2b
Maryam Golshani,Nafise Vaeznia,Mehdi Sahmani,Saeid Bouzari 대한백신학회 2016 Clinical and Experimental Vaccine Research Vol.5 No.1
Purpose: At present, there is no vaccine available for the prevention of human brucellosis. Brucella outer membrane protein 2b (Omp2b) is a 36 kD porin existed in common Brucella pathogens and it is considered as priority antigen for designing a new subunit vaccine. Materials and Methods: In the current study, we aimed to predict and analyze the secondary and tertiary structures of the Brucella abortus Omp2b protein, and to predict T-cell and B-cell epitopes with the help of bioinformatics tools. Subsequently, cloning and expression of the short form of Omp2b (SOmp2b) was performed using pET28a expression vector and Escherichia coli BL21 host, respectively. The recombinant SOmp2b (rSOmp2b) was purified with Ni-NTA column. Results: The recombinant protein was successfully expressed in E. coli host and purified under denaturation conditions. The yield of the purified rSOmp2b was estimated by Bradford method and found to be 220 μg/mL of the culture. Conclusion: Our results indicate that Omp2b protein has a potential to induce both B-cell. and T-cell.mediated immune responses and it can be evaluated as a new subunit vaccine candidate against brucellosis.
Maryam Javadi,Mana Oloomi,Saeid Bouzari 한국유전체학회 2017 Genomics & informatics Vol.15 No.2
In this study, cytolethal distending toxin (CDT) producer isolates genome were compared with genome of pathogenic and commensal Escherichia coli strains. Conserved genomic signatures among different types of CDT producer E. coli strains were assessed. It was shown that they could be used as biomarkers for research purposes and clinical diagnosis by polymerase chain reaction, or in vaccine development. cdt genes and several other genetic biomarkers were identified as signature sequences in CDT producer strains. The identified signatures include several individual phage proteins (holins, nucleases, and terminases, and transferases) and multiple members of different protein families (the lambda family, phage-integrase family, phage-tail tape protein family, putative membrane proteins, regulatory proteins, restriction-modification system proteins, tail fiber-assembly proteins, base plate-assembly proteins, and other prophage tail-related proteins). In this study, a sporadic phylogenic pattern was demonstrated in the CDT-producing strains. In conclusion, conserved signature proteins in a wide range of pathogenic bacterial strains can potentially be used in modern vaccine-design strategies.
Abdollah Khadivi-Khub,Zabihollah Zamani,Naser Bouzari 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.3
Sweet cherry (Prunus avium L.), a member of Rosaceae family, is an economically important fruit of the temperate zone. In Iran, various sweet cherry genotypes are grown in different areas. For estimation of genetic diversity, 23 RAPD decamer primers data as well as 23 morphological traits were used on 39 sweet cherry cultivars and genotypes, 28 of Iranian and 11 of foreign origin. Random Amplified Polymorphic DNA (RAPD) data was used for clustering of genotypes using UPGMA method. Based on the results, in some cases, clustering of genotypes by RAPD data was in agreement with morphological data; however, the correlation between the two sets of data was not significant (r = 0.2). The coephenitic coefficients between genotypes varied from 0.43 to 0.83 and the value of calculated polymorphism was 81.7 percent, indicating the presence of a high variation between the studied cultivars. This could be due to the presence of both Iranian and foreign genotypes in the experiment. In the main subcluster, genotypes from both origins were present and some genotypes were showing close relationships. Significant regression associations were found between 7 morphological traits and RAPD markers and some informative markers were found for the traits. Also, in clustering of genotypes good agreements were found between the subclusters and the pollination incompatibility groups reported by other workers. The results showed that RAPD is an effective maker for study of genetic diversity among sweet cherry genotypes.