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( Jia Cheng ),( Na Sun ),( Xin Zhao ),( Li Niu ),( Mei Qin Song ),( Yao Gui Sun ),( Jun Bing Jiang ),( Jian Hua Guo2 ),( Yuan Sheng Bai ),( Jun Ping He ),( Hong Quan Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8
Seventeen compounds derived from traditional Chinese medicines (TCMs) were tested for their antiviral activity against porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. Visualization with the cytopathologic effect (CPE) assay and the 3-(4, 5-dimethyithiazol- 2-yl)-2,5-diphenyltetrazolium bromide test were used to determine the 50% cytotoxic concentration (CC50) and 50% effective concentration (EC50) in cultured Marc-145 cells. Among the tested compounds, chlorogenic acid and scutellarin showed potential anti-PRRSV activity. The EC50 values were 270.8 ± 14.6 μg/ml and 28.21 ± 26.0 μg/ml and the selectivity indexes were >5.54 and 35.5, respectively. The time-of-addition and virucidal assay indicated that the anti-PRRSV activity of the two compounds could be due to their inhibiting the early stage of virus replication and/or inactivating the virus directly. The inhibition of the virus attachment was not observed in the adsorption inhibition assay. The inhibition ratios of chlorogenic acid and scutellarin were, respectively, 90.8% and 61.1% at the maximum non-cytotoxic concentrations. The results have provided a basis for further exploration of their antiviral properties and mechanisms in vivo. We believe that the chlorogenic acid and scutellarin have a great potential to be developed as new anti-PRRSV drugs for clinical application.
( Bing Guo ),( Mu Lan Jiang ),( Xia Wan ),( Yang Min Gong ),( Zhuo Liang ),( Chuan Jiong Hu ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.10
The marine microalga Isochrysis sphaerica is rich in the very-long-chain polyunsaturated fatty acids, including eicosapentaenoic acid (EPA, C20:5ω-3) and docosahexaenoic acid (DHA, C22:6ω-3) that are important to human health. Here, we report a functional characterization of a Δ4-fatty acid desaturase gene (FAD4) from I. sphaerica. IsFAD4 contains a 1,284 bp open reading frame encoding a 427 amino acid polypeptide. The deduced amino sequence comprises three conserved histidine motifs and a cytochrome b5 domain at its N-terminus. Phylogenetic analysis indicated that IsFad4 formed a unique Isochrysis clade distinct from the counterparts of other eukaryotes. Heterologous expression of IsFAD4 in Pichia pastoris showed that IsFad4 was able to desaturate docosapentaenoic acid (DPA) to form DHA, and the rate of converting DPA to DHA was 79.8%. These results throw light on the potential industrial production of specific polyunsaturated fatty acids through IsFAD4 transgenic yeast or oil crops.
Bing Guo,Qingliang Zhao,Xinyu Zhao 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.
The conditioned coarse-grained diamond wheels are able to achieve the identical surface roughness, higher form accuracy and largergrinding rate when compared to the traditional fine-grained diamond wheels. However, deeper subsurface damage could be inevitablyintroduced due to the flat tops of the coarse diamond grains. This work presents a micro-structuring method for coarse-graineddiamond wheels by means of nanosecond pulsed laser, which allows the generation of micro grooves arrays on the coarse diamondgrains distributed on the whole grinding wheel cylindrical surface. The influence of laser parameters such as focal point shift, laserpower, scanning speed and scanning passes on micro-structured surface was investigated, and then the laser machining process wasoptimized in fabricating uniform and continuous micro-structures. The grinding experiments indicate that, when compared to the nonmicro-structured grinding wheels, the subsurface cracks depth of ground optical glass was reduced effectually by using the microstructuredcoarse-grained diamond wheels.
Grinding Marks on Ultra-Precision Grinding Spherical and Aspheric Surfaces
Bing Chen,Bing Guo,Shichun Li,Zhaohui Deng,Qingliang Zhao 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.4 No.4
Grinding marks are regard as a great obstacle to manufacture spherical and aspheric surfaces with higher surface quality, lower energy and wastage. The scallop-height was studied for optimizing the grinding parameters firstly to reduce its effect on grinding marks. Secondly, the expression of grinding points distribution was established to characterize the grinding marks caused by the radial run-out of grinding wheel. And then, the aspheric grinding experiments of monocrystalline silicon were carried out to investigate the influence of grinding marks on surface quality. The experiments revealed that the remarkable grinding marks with patterned grinding points distribution would cause more fractures and roughness, deeper grooves, and more inhomogeneous surface quality compared with the weak grinding marks. The discriminating standard of grinding marks was established, and the grinding parameters were optimized for homogenizing the grinding points distribution by this discriminating standard to reduce the grinding marks in actual grinding process. Finally, the large size infrared lens was ground with high surface quality by the optimized grinding parameters, and the results of surface quality demonstrate that the discriminating standard was effective. This research provides references and ideas for grinding aspherical surface with high surface quality and efficiency, low energy and wastage.
Guo, Bing,Zhang, Yiming,Zhang, Jialin,Gao, Junxia The Korean Institute of Power Electronics 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.3
A digital direct phase-shift control (DDPSC) method based on the phase-shifted full-bridge LLC (PSFB-LLC) converter is presented. This work combines DDPSC with the conventional linear control to obtain a hybrid control strategy that has the advantages of linear control and DDPSC control. The strategy is easy to realize and has good dynamic responses. The PSFB-LLC circuit structure is simple and works in the fixed frequency mode, which is beneficial to magnetic component design; it can realize the ZVS of the switch and the ZCS of the rectifier diode in a wide load range. In this work, the PSFB-LLC converter resonator is analyzed in detail, and the concrete realization scheme of the hybrid control strategy is provided by analyzing the state-plane trajectory and the time-domain model. Finally, a 3 kW prototype is developed, and the feasibility and effectiveness of the DDPSC controller and the hybrid strategy are verified by experimental results.
Bing Guo,Yiming Zhang,Jialin Zhang,Junxia Gao 전력전자학회 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.3
A digital direct phase-shift control (DDPSC) method based on the phase-shifted full-bridge LLC (PSFB-LLC) converter is presented. This work combines DDPSC with the conventional linear control to obtain a hybrid control strategy that has the advantages of linear control and DDPSC control. The strategy is easy to realize and has good dynamic responses. The PSFB-LLC circuit structure is simple and works in the fixed frequency mode, which is beneficial to magnetic component design; it can realize the ZVS of the switch and the ZCS of the rectifier diode in a wide load range. In this work, the PSFB-LLC converter resonator is analyzed in detail, and the concrete realization scheme of the hybrid control strategy is provided by analyzing the state-plane trajectory and the time-domain model. Finally, a 3 kW prototype is developed, and the feasibility and effectiveness of the DDPSC controller and the hybrid strategy are verified by experimental results.
Liu, Guo-Bing,Qu, Yan-Juan,Liao, Mei-Yan,Hu, Hui-Juan,Yang, Gui-Fang,Zhou, Su-Jun Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11
Objective: To explore the relationship between computed tomography (CT) manifestations of thymoma and its WHO pathological classification. Methods: One hundred and five histopathologically confirmed cases were collected for their pathological and CT characteristics and results were statistically compared between different pathological types of thymoma. Results: Tumor size, shape, necrosis or cystic change, capsule integrity, invasion to the adjacent tissue, lymphadenopathy, and the presence of pleural effusion were significantly different between different pathological types of thymomas (P<0.05). Type B2, B3 tumors and thymic carcinomas were greater in size than other types. More than 50% of type B3 tumors and thymic carcinomas had a tumor size greater than 10 cm. The shape of types A, AB, and B1 tumors were mostly round or oval, whereas 75% of type B3 tumors and 85% of thymic carcinomas were irregular in shape. Necrosis or cystic change occurred in 67% of type B3 thymomas and 57% of thymic carcinomas, respectively. The respective figures for capsule destruction were 83% and 100%. Increases in the degree of malignancy were associated with increases in the incidence of surrounding tissue invasion: 33%, 75%, and 81% in type B2, type B3, and thymic carcinomas, respectively. Pleural effusion occurred in 48% of thymic carcinomas, while calcification was observed mostly in type B thymomas. Conclusions: Different pathological types of thymic epithelial tumors have different CT manifestations. Distinctive CT features of thymomas may reflect their pathological types.