A SOLUTION TO AN OPEN PROBLEM ON REVERSE TRIGONOMETRIC MASJED-JAMEI INEQUALITY

Bhukya Ravi,A. Venkata Lakshmi,Yogesh J. Bagul 충청수학회 2022 충청수학회지 Vol.35 No.1

In this short note, we prove an open problem for the interval (-∞, ∞), related to a reverse trigonometric Masjed-Jamei inequality presented in [2] and establish a new inequality of a similar kind.

Identification of High Affinity Non-Peptidic Small Molecule Inhibitors of MDM2-p53 Interactions through Structure-Based Virtual Screening Strategies

Bandaru, Srinivas,Ponnala, Deepika,Lakkaraju, Chandana,Bhukya, Chaitanya Kumar,Shaheen, Uzma,Nayarisseri, Anuraj Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Background: Approaches in disruption of MDM2-p53 interactions have now emerged as an important therapeutic strategy in resurrecting wild type p53 functional status. The present study highlights virtual screening strategies in identification of high affinity small molecule non-peptidic inhibitors. Nutlin3A and RG7112 belonging to compound class of Cis-imidazoline, MI219 of Spiro-oxindole class and Benzodiazepine derived TDP 665759 served as query small molecules for similarity search with a threshold of 95%. The query molecules and the similar molecules corresponding to each query were docked at the transactivation binding cleft of MDM2 protein. Aided by MolDock algorithm, high affinity compound against MDM2 was retrieved. Patch Dock supervised Protein-Protein interactions were established between MDM2 and ligand (query and similar) bound and free states of p53. Compounds with PubCid 68870345, 77819398, 71132874, and 11952782 respectively structurally similar to Nutlin3A, RG7112, Mi219 and TDP 665759 demonstrated higher affinity to MDM2 in comparison to their parent compounds. Evident from the protein-protein interaction studies, all the similar compounds except for 77819398 (similar to RG 7112) showed appreciable inhibitory potential. Of particular relevance, compound 68870345 akin to Nutlin 3A had highest inhibitory potential that respectively showed 1.3, 1.2, 1.16 and 1.26 folds higher inhibitory potential than Nutilin 3A, MI 219, RG 7112 and TDP 1665759. Compound 68870345 was further mapped for structure based pharamacophoric features. In the study, we report Cis-imidazoline derivative compound; Pubcid: 68870345 to have highest inhibitory potential in blocking MDM2-p53 interactions hitherto discovered.

Effect of Supplementing Organic Selenium on Performance, Carcass Traits, Oxidative Parameters and Immune Responses in Commercial Broiler Chickens

Rao, Savaram Venkata Rama,Prakash, Bhukya,Raju, Mantena Venkata Laxmi Narasimha,Panda, Arun Kumar,Poonam, Saharia,Murthy, Orugonda Krishna Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.2

An experiment was conducted to determine the effect of supplementing various concentrations (0, 100, 200, 300, or 400 ${\mu}g/kg$ diet) of organic Se on growth performance, carcass traits, oxidative stress, and immune responses in commercial broiler chickens reared in open-sided poultry house under tropical climatic conditions. Each diet was fed ad libitum to eight replicates consisting of six birds in each pen from 1 to 42 d of age. Body weight gain and feed efficiency, and relative weight of liver, abdominal fat and ready to cook yields were not affected (p>0.05) by organic Se supplementation to broiler diets. Lipid peroxidation in plasma decreased, while activities of glutathione peroxidase and glutathione reductase in plasma increased (p<0.01) linearly with Se concentration in diet. The ratios between heterophyls and lymphocytes and relative weight of lymphoid organs (bursa, spleen, and thymus), and antibody production to Newcastle disease vaccination were not affected (p>0.05) by Se supplementation to broiler diets. However, the cell-mediated immunity (lymphocyte proliferation ratio) increased (p<0.01) linearly with dietary Se concentration. The results of the present study indicate that the supplementation of Se did not influence body weight and feed efficiency. However, supplementation of Se increased antioxidant status and lymphocyte proliferation in broiler chickens.

Biochar Impact on Methane Generation and Nitrogen Dynamics in Ruminal Fermentation

Zhengxia Dou,Dipti Pitta,John Toth,Bonnie Vecchiarelli,Bhima Bhukya,Mingxia Guo,James Ferguson 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Effect of methyl donors supplementation on performance, immune responses and anti-oxidant variables in broiler chicken fed diet without supplemental methionine

Savaram Venkata Rama Rao,Raju Mantena Venkata Lakshmi Narasimha,Bhukya Prakash,Paul Shyam Sunder,Devanaboyina Nagalakshmi 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.3

Objective: Methionine (Met) is involved in methyl group transfer besides protein synthesis. As the availability is limited and cost is high for synthetic Met, reductions in its inclusion in broiler diet may be possible by supplementing the low Met diets with methyl donors (MD) like betaine (Bet), folic acid (FA), vitamin B12 (B12), and biotin (Bio). An experiment was conducted to study the effects of supplementing the MD on performance (average daily gain [ADG], daily feed intake, feed efficiency [FE]), anti-oxidant variables, immune responses and serum protein concentration in broilers fed sub-optimal concentrations of dietary Met. Methods: Maize-soybean meal diet was used as control (CD). Different MD like Bet (0.2%), B12 (0.1 mg), FA (4 mg), or Bio (1.5 mg/kg) were supplemented to basal diet (BD) having no supplemental Met. The BD without MD was kept for comparison. Each diet was fed ad libitum to 10 replicates of 25 chicks in each from 1 to 42 d of age. Results: At the end of experiment, the ADG in MD group was higher than BD and lower than CD. The FE improved with FA or Bet compared to the BD. Breast meat weight was higher in Bet compared to the BD, while it was intermediate between BD and CD in other groups. The lipid peroxidation reduced with Bio, B12, or Bet, while the glutathione peroxidase activity improved with Bio or B12 compared to the BD. Lymphocyte proliferation improved with Bet compared to the BD. The serum protein concentrations increased with FA, Bio, or Bet compared to those fed BD. Conclusion: It can be concluded that the ADG can be improved partially with supplementation of MD while the FE improved with FA or Bet. Some MD also reduced the stress indices and improved immune responses compared to the BD fed broilers. Objective: Methionine (Met) is involved in methyl group transfer besides protein synthesis. As the availability is limited and cost is high for synthetic Met, reductions in its inclusion in broiler diet may be possible by supplementing the low Met diets with methyl donors (MD) like betaine (Bet), folic acid (FA), vitamin B12 (B12), and biotin (Bio). An experiment was conducted to study the effects of supplementing the MD on performance (average daily gain [ADG], daily feed intake, feed efficiency [FE]), anti-oxidant variables, immune responses and serum protein concentration in broilers fed sub-optimal concentrations of dietary Met.Methods: Maize-soybean meal diet was used as control (CD). Different MD like Bet (0.2%), B12 (0.1 mg), FA (4 mg), or Bio (1.5 mg/kg) were supplemented to basal diet (BD) having no supplemental Met. The BD without MD was kept for comparison. Each diet was fed ad libitum to 10 replicates of 25 chicks in each from 1 to 42 d of age.Results: At the end of experiment, the ADG in MD group was higher than BD and lower than CD. The FE improved with FA or Bet compared to the BD. Breast meat weight was higher in Bet compared to the BD, while it was intermediate between BD and CD in other groups. The lipid peroxidation reduced with Bio, B12, or Bet, while the glutathione peroxidase activity improved with Bio or B12 compared to the BD. Lymphocyte proliferation improved with Bet compared to the BD. The serum protein concentrations increased with FA, Bio, or Bet compared to those fed BD.Conclusion: It can be concluded that the ADG can be improved partially with supplementation of MD while the FE improved with FA or Bet. Some MD also reduced the stress indices and improved immune responses compared to the BD fed broilers.

Enhancement of performance and anti-oxidant variables in broiler chicken fed diets containing sub-optimal methionine level with graded concentrations of sulphur and folic acid

Savaram, Venkata Rama Rao,Mantena, Venkata Lakshmi Narasimha Raju,Paul, Shyam Sunder,Devanaboyina, Nagalakshmi,Thota, Srilatha,Bhukya, Prakash,Ullengala, Rajkumar Asian Australasian Association of Animal Productio 2022 Animal Bioscience Vol.35 No.5

Objective: An experiment was conducted to determine the effects of supplementing graded concentrations of inorganic sulphur (S) without and with folic acid (FA) in maize-soybean meal diets on performance, slaughter and anti-oxidant variables, immune responses and serum protein fractions in broiler chicken. Methods: Inorganic S was supplemented at 0.05%, 0.10%, 0.15%, and 0.20% alone or in combination with FA (4 mg/kg) in basal diet (BD) containing no supplemental methionine (Met) and FA. A control group was fed with the recommended concentration of Met. Each diet was offered to 10 pens of 5 male broiler chicks (Cobb 400) and fed ad libitum from day 1 to 42. Results: The broilers fed the BD had lower body weight gain (BWG), feed efficiency (FE), higher lipid peroxidation (LP), lower activity of glutathione peroxidase (GSHPx), lower lymphocyte proliferation ratio (LPR), and reduced concentrations of total protein, albumin, and globulin in serum. Supplementation of FA and S to the BD improved the BWG (all concentrations of S) and FE (0.20% S) similar to the control group. Similarly, the combination of S and FA significantly improved the concentrations of total protein, albumin, and globulin in serum, reduced the LP and increased the activity of GSHPx and LPR. However, responses in the above parameters were related to the concentration of S in the diet. The slaughter variables and antibody titres against the Newcastle disease were not affected with the treatments. Conclusion: Based on the results, it is concluded that the combination of S (0.2%) and FA (4 mg/kg) improved the BWG and FE, similarly supplementation of these nutrients improved the concentration of protein fractions and reduced the stress (reduced LP and improved GSHPx) variables in serum and improved the cell mediated immune response (LPR) in broilers fed sub-optimal concentrations of Met in diet.

Graded concentrations of digestible lysine on performance of White Leghorn laying hens fed sub-optimal levels of protein

Savaram, Venkata Rama Rao,Paul, Shyam Sundar,Mantina, Venkata Lakshmi Narasimha Raju,Devanaboyina, Nagalakshmi,Bhukya, Prakash Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.5

Objective: An experiment was conducted to study the effect of graded concentration of digestible lysine (dLys) on performance of layers fed diets containing sub-optimal level of protein. Methods: Five diets were formulated to contain graded concentrations of dLys (0.700%, 0.665%, 0.630%, 0.593%, and 0.563%), but similar levels of crude protein (15% CP), energy (10.25 MJ ME/kg) and other nutrients. A total of 3,520 hens (26 wk of age) with mean body weight of 1,215+12.65 g were randomly divided into 40 replicate groups of 88 birds in each and housed in an open sided colony cage house. Each diet was offered ad libitum to eight replicates from 27 to 74 wk of age. The performance was compiled at every 28 d and the data for each parameter were grouped into three phases, that is early laying phase (27 to 38 wk), mid laying phase (39 to 58 wk), and late laying phase (59 to 74 wk of age) for statistical analysis. Results: Egg production, egg mass and feed efficiency (feed required to produce an egg) were significantly improved by the dLys level during the early and mid laying phases but not during the late phase. Whereas feed intake was significantly reduced by dLys concentration during mid and late laying phases but not during early laying phase. The egg weight was not affected by dLys concentration in any of the three phases. Conclusion: Based on best fitted statistical models, dietary requirements of dLys worked out to be 0.685%, 0.640%, and 0.586% during early phase, mid phase, and late egg laying phase, respectively. The calculated requirement of dLys for the respective production phases are 727 mg/b/d during the early and mid laying phases and 684 mg/b/d during the late laying phase in diets containing 15% CP.

Identification of a novel QTL for grain number per panicle employing NGS-based QTL-seq approach in rice (Oryza sativa L.)

Bommisetty Reddyyamini,Chakravartty Navajeet,Hariprasad K. R.,Rameshbabu P.,Sudhakar P.,Bodanapu Reddaiah,Naik Jeevula Bhukya,Reddy B. V. Bhaskar,Lekkala Sivrama Prasad,Gupta Saurabh,Tanti Bhaben,Lach 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.2

The grain number is one of the highly complex and crucial traits determining the grain yield in rice. To identify grain number QTLs in rice, we employed the QTL-seq approach in the 297 F2 population derived from the cross between BPT5204 and NLR33892. In this investigation, five regions viz., qGN1, qGN3, qGN7, qGN9, and qGN12 on chromosomes 1,3,7,9, and 12, respectively, were identified as QTLs governing grain number per panicle in rice. To verify the QTLs identified in the present study, traditional QTL mapping was carried out using InDel markers and previously reported SSRs in the QTL region. From qGN12 QTL, RM6953 showed a significant association with the number of grains per panicle with a phenotypic variance of 24.58, 22.10, and 17.20% in F2, F2:3, and BC1F2 populations, respectively, at < 0.0001 P value, indicating major QTL. From genome resequencing data, a missense variance was observed in the exonic region of LOC_Os12g39330 gene controlling AP2 domain-containing protein which plays an important role in seed development in rice. Hence, this QTL can be a potential target for map-based cloning and marker-assisted transfer to enhance the grain number in rice.