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Bao‑Jian Zhu,Lin Tang,Ying‑Ying Yu,Dao‑Jun Wang,Chao‑Liang Liu 한국유전학회 2017 Genes & Genomics Vol.39 No.6
Ecdysteroids play an important role in the regulation of molting process in crustaceans. The suppression subtractive hybridization (SSH) technique was employed to search for 20-hydroxyecdysone-responsive genes in the hepatopancreas of the crayfish Procambarus clarkii. Ninety-three putative expressed sequence tags were identified by SSH, including three immune response-related genes, two cell cycle and apoptosis genes, four respiration and energy metabolism genes, four transport-related genes, six metabolism-related genes, two stress response genes, and eight transcription and translation regulation genes. The expression levels of the examined genes were regulated by 20-hydroxyecdysone and varied in the hepatopancreas during the molting stages; data were confirmed by real-time PCR. RNAi of the ecdysteroid receptor had significant effects on the expression levels of 20-hydroxyecdysone- responsive genes. These differentially expressed genes identified by SSH will provide insight into the ecdysteroid signaling pathway in P. clarkii.
In Vitro Inhibitory Effect of Triterpenoidal Saponins from Platycodi Radix on Pancreatic Lipase
Bao Jun Xu,Li Kun Han,Yi Nan Zheng,Jeong Hyun Lee,Chang Keun Sung 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.2
In the process of investigating anti-obesity effect of Platycodi Radix, we found that aqueous extract of Platycodi Radix might inhibit intestinal absorption of dietary fat by inhibiting pancreatic lipase (PL) activity. In order to clarify the anti-obesity mechanism of Platycodi Radix, activity- guided isolation was performed to find active components. The total saponin fraction of Platycodi Radix appeared to have a potent inhibitory activity against the hydrolysis of triolein emulsified with phosphatidycholine by pancreatic lipase in vitro. Based on these results, further purification of active components yielded 10 known triterpenoidal saponins, among these compounds, platycodin A, C, D, and deapioplatycodin D exhibited significant inhibitory effects on PL at the concentration of 500 µg/mL with 3.3, 5.2, 34.8, and 11.67% pancreatic lipase activity vs control, respectively. Platycodin D was found to inhibit the PL activity in a dosedependent manner. Therefore, the anti-obesity effect of Platycodi Radix might be due to the inhibition of pancreatic lipase by its saponins.
Organization process of the hierarchical structures in microbially synthesized polyhydroxyalkanoates
Jun Xu,Bao-Hua Guo,Qiong Wu,Jin-Chun Chen,Guo-Qiang Chen,Jian-Jun Zhou,Yong Jiang,Lin Li 한국물리학회 2007 Current Applied Physics Vol.7 No.s1
cess of the high-order structures in biomaterials. Real-time Fourier transform infrared spectroscopy and optical microscopy demon-strated that helical segments formed along with the spherulite growth. Atomic force microscopy revealed the details of growth,twisting and branching of lamellar crystals. Cooperative packing of these twisting lamellae led to regular banded spherulites observedunder polarized light microscopy. Real-time observation on the crystallization process provided richer information than the characterization of the final structures; consequently, it provides deeper insight into the organization mechanism of the hierarchical structures.
Bao Jun Liu,임소정 한국중국어교육학회 2023 중국어교육과연구 Vol.- No.40
In Modern Chinese grammar, it is stipulated that the locative object following a verb can only be placed in between the compound directional complement. However, there is a significant phenomenon of the locative object appearing after the compound directional complement in our surroundings. How is this ungrammatical word order phenomenon distributed and what differences exist between it and the normal grammatical expression? Based on previous research, this article presents a comparative analysis of a large corpus of data, enumerating the distribution and syntactic differences between two types of expressions. For instance, the verb category represented by "进" and "回" tends to use the intermediate-object structure, while the verb category represented by "出" and "过" tends to use the post-object structure in the compound directional complement. From an information structure perspective, the intermediate-object structure is often employed to emphasize the locative object at the end of a sentence, while the post-object structure is frequently used in serial verb constructions to highlight the following constituent. Through an analysis of the corpus and a comparison with actual usage through questionnaire surveys, this article discovers that the phenomenon of placing the locative object after the compound directional complement deserves our attention. It is not simply ungrammatical sentences but rather a special word order with usage patterns and tendencies. Moreover, to some extent, it can fulfill the needs of specific syntax and semantics. 现代汉语语法中规定动词后处所宾语只能用在复合趋向补语的中间使用,但在我们周围存在大量处所宾语用在复合趋向补语后的语序现象。这种不合语法的语序现象是如何分布的且与正常的语法表述之间又存在什么样的差异呢?本文在前人研究的基础上,对语料库中大量语料对比分析列举出两种表述格式的分布情况和语序差异。如“进、回”字类动趋式多用中宾式表述;“出、过”字类动趋式多用后宾式表述。从信息结构来看中宾式多用在句末突出处所宾语,而后宾式多用在连动句中突出后面成分。本文在对语料库分析的同时通过问卷调查与人们实际的使用情况对比发现,处所宾语用在复合趋向补语后的语序现象值得我们关注,其并非单纯的病句,而是有使用规律和倾向性的特殊语序,且在一定程度上能满足特殊句法和语义的需要。
Chemical Constituents and Pharmacological Activities of Hedyotis diffusa
Bao-Jun Xu,성창근 한국생약학회 2005 Natural Product Sciences Vol.11 No.1
The chemical constituents from Hedyotis difusa Wild and their pharmacological activities weresummarized. It has been known data that this herb contains anthraquinones, terpenoids, steroids, flavonoids,organic acid, and polysaccharides. The studies of pharmacology have shown that Hedyotis difusa Wild possessvarious levels of activities such as anticancer, anti-inflammatory, immunostimulatory, antioxidative, neuroprotective,and hepatoprotective activities. Keywords Hedyotis difusa, chemical constituents, pharmacological activities