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Cell cycle arrest mediated by WEE1 is involved in the unfolded protein response in plants
고기성,유재용,Nirmal Kumar Ramasamy,RIKNOHARMOKO,Bích Ngọc Thị Vũ,박지예,이균오 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.5
Activation of the unfolded protein response (UPR) in mammalian cells leads to cell cycle arrest at the G1 phase (Thomas et al., J Biol Chem 288:7606–7617, 2013). However, how UPR signaling affects cell cycle arrest remains largely unknown in plants. Here, we examined UPR and endoreduplication in Col-0, wee1, and ER stress sensing-deficient ire1a&b plants during DNA replication and ER stress conditions. We found that WEE1, an essential negative regulator of the cell cycle, is involved in the maintenance of ER homeostasis during genotoxic stress and the ER stress hypersensitivity of ire1a&b is alleviated by loss-of-function mutation in WEE1. WEE1-mediated cell cycle arrest was required for IRE1–bZIP60 pathway activation during ER stress. In contrast, loss-of-function mutation in WEE1 caused increased expression of UPR-related genes during DNA replication stress. WEE1 and IRE1 were required for endoreduplication during DNA replication stress and ER stress, respectively. Taken together, these findings suggest that cell cycle regulation is associated with UPR activation in different manners during ER stress and DNA replication stress in Arabidopsis.
Customized N-glycosylation for the production of glucocerebrosidase in Arabidopsis
이균오,Ki Seong Ko,Jae Yong Yoo,Bích Ngọc Thị Vũ,Ji Ye Park 한국당과학회 2021 한국당과학회 학술대회 Vol.2021 No.01
Plants and plant cells are evolving with improved safety and production as attractive options for biopharmaceutical production. A significant barrier to the development of biopharmaceuticals in plants, however, lies in the fact that plant-derived N-glycans contain plant-specific sugar residues such as β1,2-xylose and α1,3-fucose bound to the pentasaccharide core (Man3GlcNAc2) as well as β1,3-galactose and α1,4-fucose involved in the formation of Lewis a (Lea) epitope that may cause allergic reactions in humans. Additionally, sugar residues such as α1,6-fucose, β1,4-galactose, and α2,6-sialic acid, which are believed to play important roles in biopharmaceutical action, storage, distribution, and half-life, are missing from the naturally occurring N-glycans in plants. To use plant cells as a means of producing biopharmaceuticals, it is essential to produce plants which contain N-glycan compatible with biopharmaceuticals. However, the structure of N-glycans appears to be related to hormone signalling and how the structure of N-glycans altered during glycoengineering influences plant production is still uncertain. Here, we suggest a strategy for producing customized N-glycans in plants and the related technological barriers.