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( Ashok Badigannavar ),( Ashok Kumar ),( G. Girish ),( T. R. Ganapathi ) 한국육종학회 2017 Plant Breeding and Biotechnology Vol.5 No.2
Sorghum is a major staple crop and vital for the marginal farmers in Asian and African countries. Landraces or germplasm lines adapted to biotic and abiotic stresses are the prime source of adaptive traits in the crop breeding programs. In order to assess the genetic variability, 141 exotic germplasm lines and 36 popular varieties were evaluated for eight agro-morphological traits. Wide range of values were observed for grain yield (6.6-124.4 g/plant), seed index (1.5-7.1 g/100 seeds), panicle length (8-44 cm) and panicle width (7.25 cm). High genotypic and phenotypic coefficient of variation was observed for grain yield per plant. The values of broad sense heritability in the sorghum core population ranged from 0.75 to 0.99. Seed weight, days to flowering, panicle width and leaf area were significantly correlated with grain yield (P ≤ 0.01). Seed weight was positively correlated with panicle width (correlation coefficient 0.31), while negatively correlated with panicle length (correlation coefficient -0.15). Cluster analysis resolved all the genotypes into four major clusters. Among germplasm lines, TSG-313 had high seed weight of 7.05 g/100 seeds, while TSG-325 had highest grain yield of 124.4 g/plant as against control variety. Germplasm lines with high heritability scores would help us to utilise them in recombination breeding.
Cell death in culture: Molecular mechanisms, detections, and inhibition strategies
Anandrao Ashok Patil,Sachin Ashok Bhor,Won Jong Rhee 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.91 No.-
Mammalian cell cultures are widely used in the biopharmaceutical industry to produce monoclonalantibodies, vaccines, growth factors, etc. Cell death is an essential biological process for physiologicalgrowth and development, but it is a major problem in biopharmaceutical production in bio-industry. Celldeath within bioreactor occurs due to various intracellular and extracellular stresses. These stressesnegatively affect the culture longevity, overall product quality, and yield. Among all cell death types,apoptosis accounts for most of the cellular death in the bioreactor. The implementation and developmentof various strategies to prevent the cellular death are crucial for robust bioprocess development. Celldeath during culture can be prevented or inhibited by supplementing media with specific chemicals,synthetic inhibitors, and genetic cell engineering approaches. In this review, we classified and describeddifferent types of cell death and their molecular mechanisms and summarized the cell death inhibitionapproaches implemented to inhibit cell death for various applications.
Effective D-shape fiber with air hole assistant design for birefringence analysis
Ashok, Nandam,Shin, WooJin Elsevier 2018 OPTIK -STUTTGART- Vol.162 No.-
<P><B>Abstract</B></P> <P>We propose an elliptic core effective D-shaped fiber with air hole assistant for birefringence applications. The proposed birefringent fiber works on the principle of asymmetric core region. The birefringence of the proposed D-shaped fiber has been numerically investigated in detail. The designed fiber has effective mode areas of 16.04 μm<SUP>2</SUP> and 16.06 μm<SUP>2</SUP> for <I>x-</I>and <I>y</I>-polarization modes, respectively, at a wavelength of 1550 nm. Our numerical results reveal a birefringence and confinement loss of 2.25 × 10<SUP>−4</SUP> and 6.66 <B>×</B> 10<SUP>−3</SUP> dB/m respectively at 1550 nm wavelength. The proposed fiber exhibits a dispersion variation of 1.3 ps/km-nm and 1.57 ps/km-nm respectively for <I>x-</I>and <I>y</I>-polarizations over a wavelength band of 200 nm ranging from 1.4 to 1.6 μm. We have also investigated the influence of variation in the fiber parameters on the birefringence and dispersion. Furthermore, we have calculated the fabrication tolerances, the variation of birefringence value is very small by varying the air hole diameters of the fiber. We believe that the reported results have potential importance in the design and development of polarization control devices applicable in fiber-optic sensors.</P>
Ashok Khanna,Debjit Sanpui,Manish K. Singh 한국화학공학회 2004 Korean Journal of Chemical Engineering Vol.21 No.2
Mass transfer studies in a laboratory scale extraction column have been conducted for Toluene-Acetone-Water and MIBK-Acetic Acid-Water systems. From these experiments stage-wise solute (Acetone or Acetic-Acid)composition profiles have been obtained for both dispersed and continuous phase. These composition profiles havebeen compared with those obtained from ASPENPLUS, CHEMSEP and LLXSIM simulators. For liquid-liquid equilib-rium calculations all these simulators use UNIFAC and UNIQUAC model. The binary interaction parameters for theUNIFAC are inbuilt in ASPENPLUS and CHEMSEP. UNIQUAC binary parameters were borowed from DECHEMA.Eror square analysis indicates that simulations based on non-equilibrium option of LLXSIM match closely withexperimental results. Temperature profiles and hydrodynamic features characterized by number of drops and staticholdup on the stages have been compared between the LLXSIM simulated and the experimental results and these matchwel. However simulations on ASPENPLUS give sum of relative error-squares for all the experimental runs at least tentimes higher, in spite of tuning the average stage efficiency.
Right Atrial Paraganglioma: An Extremely Rare Primary Cardiac Neoplasm Mimicking Myxoma
Ashok Garg,Deepika Mishra,Manish Bansal,Hari Ram Maharia,Vikram Goyal 한국심초음파학회 2016 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.24 No.4
In this report, we present a case of 35-year-old lady who had presented with atypical chest pain and exertional breathlessness forpast six months. Transthoracic and transesophageal echocardiograms showed a well-circumscribed, echo-dense mass in the rightatrium, attached to the interatrial septum at the level of atrioventricular junction and in the vicinity of coronary sinus ostium. Sheunderwent successful resection of the cardiac mass. Histopathology revealed paraganglioma, which was reconfirmed by immunohistochemistrystudy. This represents an extremely rare presentation as primary cardiac tumors are 20-times less common thanmetastatic tumors and paraganglioma is one of the rarest primary cardiac tumors, accounting for < 1% of all cases.
A Schematic Overview of the Current Status of Male Infertility Practice
Ashok Agarwal,Ahmad Majzoub,Neel Parekh,Ralf Henkel 대한남성과학회 2020 The World Journal of Men's Health Vol.38 No.3
The practice of male infertility has dramatically evolved over the past few decades. This has been achieved after the realization of the important contribution the male counterpart holds in the couple’s infertility. It is also supported by a number of breakthroughs in the diagnosis and treatment of this medical condition. Several tests to investigate sperm quality and function were introduced along with refinements and/or institution of novel surgical techniques that can correct several causes of infertility. This manuscript is aimed at highlighting the current state of male infertility practice in a robust, schematic method addressing a broader audience involved in the treatment of male infertility.
Effect of Oxidative Stress on Male Reproduction
Ashok Agarwal,Gurpriya Virk,Chloe Ong,Stefan S du Plessis 대한남성과학회 2014 The World Journal of Men's Health Vol.32 No.1
Infertility affects approximately 15% of couples trying to conceive, and a male factor contributes to roughly half of these cases. Oxidative stress (OS) has been identified as one of the many mediators of male infertility by causing sperm dysfunction. OS is a state related to increased cellular damage triggered by oxygen and oxygen-derived free radicals known as reactive oxygen species (ROS). During this process, augmented production of ROS overwhelms the body’s antioxidant defenses. While small amounts of ROS are required for normal sperm functioning, disproportionate levels can negatively impact the quality of spermatozoa and impair their overall fertilizing capacity. OS has been identified as an area of great attention because ROS and their metabolites can attack DNA, lipids, and proteins; alter enzymatic systems; produce irreparable alterations; cause cell death; and ultimately, lead to a decline in the semen parameters associated with male infertility. This review highlights the mechanisms of ROS production, the physiological and pathophysiological roles of ROS in relation to the male reproductive system, and recent advances in diagnostic methods; it also explores the benefits of using antioxidants in a clinical setting.