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Suitable reference genes for relative quantification of miRNA expression in prostate cancer
Annika Schaefer,Monika Jung,Kurt Miller,Michael Lein,Glen Kristiansen,Andreas Erbersdobler,Klaus Jung 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.11
Real time quantitative PCR (qPCR) is the method of choice for miRNA expression studies. For relative quantification of miRNAs, normalization to proper reference genes is mandatory. Currently, no validated reference genes for miRNA qPCR in prostate cancer are available. In this study, the expression of four putative reference genes (hsa-miR-16, hsa-miR-130b, RNU6-2,SNORD7) was examined with regard to their use as normalizer. After SNORD7 was already shown an inappropriate reference gene in preliminary experiments using total RNA pools, we studied the expression of the putative reference genes in tissue and normal adjacent tissue sample pairs from 76 men with untreated prostate carcinoma collected after radical prostatectomy. hsa-miR-130b and RNU6-2 showed no significantly different expression between the matched malignant and non-malignant tissue samples, whereas hsa-miR-16was significantly underexpressed in malignant tissue. Softwares geNorm and Normfinder predicted hsamiR-130b and the geometric mean of hsa-miR-130b and RNU6-2 as the most stable reference genes. Normalization of the four miRNAs hsa-miR-96, hsamiR-125b, hsa-miR-205, and hsa-miR-375, which were previously shown to be regulated, shows that normalization to hsa-mir-16 can lead to biased results. We recommend using hsa-miR-130b or the geometric mean of hsa-miR-130b and small RNA RNU6-2 for normalization in miRNA expression studies of prostate cancer.
Annika Wilke,Richard Brans,Kathrin Nordheider,Antje Braumann,Anja Hübner,Flora K. Sonsmann,Swen M. John,Britta Wulfhorst 한국산업안전보건공단 산업안전보건연구원 2018 Safety and health at work Vol.9 No.4
Background: Occupational skin diseases (OSDs) are frequent in professions with exposure to skin hazards. Thus, a health educational intervention for apprentices of high-risk professions was conducted. It was the aim of this study to gain insight into possible effects of this intervention. Methods: A one-time skin protection seminar was conducted in 140 apprentices of health-related and non-healtherelated professions [trained cohort (TC)]. In addition, 134 apprentices of the same occupations were monitored [untrained cohort (UTC)]. The OSD-specific knowledge and the skin condition of the hands were assessed at baseline (T0), after the seminar (T1), and after 6 (T2) and 12 months (T3). Results: The OSD-specific knowledge increased in all cohorts from T0 to T3, but we found a significantly higher knowledge in the TC at T2 (p < 0.001, t = 3.6, df = 196, 95% confidence interval ¼ 0.9, 3.3) and T3 (p < 0.001, t = 3.8, df = 196, 95% confidence interval = 1.0, 3.2) compared to the UTC. Our results indicated a better skin condition of the hands in the TC of the health-related professions but not in the non-healtherelated professions. Conclusion: The study indicates that an educational intervention may positively influence the diseasespecific knowledge and the prevalence of OSD in apprentices. However, definite conclusions cannot be drawn because of the heterogeneous study cohorts and the study design. Future research should aim at tailoring primary prevention to specific target groups, e.g., in view of the duration and frequency of skin protection education, different professions, and gender-specific prevention approaches.
PRC2 loss amplifies Ras signaling in cancer
Baude, Annika,Lindroth, Anders M,Plass, Christoph Nature Pub. Co 2014 Nature genetics Vol.46 No.11
The histone-modifying PRC2 complex has an ambiguous role in cancer, bearing both oncogenic and tumor-suppressive features depending on cell type. Studies of malignant peripheral nerve sheath tumors (MPNSTs) have now identified loss-of-function mutations altering PRC2 subunits, leading to the amplification of Ras-driven transcription and conferring vulnerability to BRD4 inhibitors.
Wilke, Annika,Brans, Richard,Nordheider, Kathrin,Braumann, Antje,Hubner, Anja,Sonsmann, Flora K.,John, Swen M.,Wulfhorst, Britta Occupational Safety and Health Research Institute 2018 Safety and health at work Vol.9 No.4
Background: Occupational skin diseases (OSDs) are frequent in professions with exposure to skin hazards. Thus, a health educational intervention for apprentices of high-risk professions was conducted. It was the aim of this study to gain insight into possible effects of this intervention. Methods: A one-time skin protection seminar was conducted in 140 apprentices of health-related and non-health-related professions [trained cohort (TC)]. In addition, 134 apprentices of the same occupations were monitored [untrained cohort (UTC)]. The OSD-specific knowledge and the skin condition of the hands were assessed at baseline (T0), after the seminar (T1), and after 6 (T2) and 12 months (T3). Results: The OSD-specific knowledge increased in all cohorts from T0 to T3, but we found a significantly higher knowledge in the TC at T2 (p < 0.001, t = 3.6, df = 196, 95% confidence interval = 0.9, 3.3) and T3 (p < 0.001, t = 3.8, df = 196, 95% confidence interval = 1.0, 3.2) compared to the UTC. Our results indicated a better skin condition of the hands in the TC of the health-related professions but not in the non-health-related professions. Conclusion: The study indicates that an educational intervention may positively influence the disease-specific knowledge and the prevalence of OSD in apprentices. However, definite conclusions cannot be drawn because of the heterogeneous study cohorts and the study design. Future research should aim at tailoring primary prevention to specific target groups, e.g., in view of the duration and frequency of skin protection education, different professions, and gender-specific prevention approaches.
Suitable reference genes for relative quantification of miRNA expression in prostate cancer
Schaefer, Annika,Jung, Monika,Miller, Kurt,Lein, Michael,Kristiansen, Glen,Erbersdobler, Andreas,Jung, Klaus Korean Society for Biochemistry and Molecular Bion 2010 Experimental and molecular medicine Vol.42 No.11
Real time quantitative PCR (qPCR) is the method of choice for miRNA expression studies. For relative quantification of miRNAs, normalization to proper reference genes is mandatory. Currently, no validated reference genes for miRNA qPCR in prostate cancer are available. In this study, the expression of four putative reference genes (hsa-miR-16, hsa-miR-130b, RNU6-2, SNORD7) was examined with regard to their use as normalizer. After SNORD7 was already shown an inappropriate reference gene in preliminary experiments using total RNA pools, we studied the expression of the putative reference genes in tissue and normal adjacent tissue sample pairs from 76 men with untreated prostate carcinoma collected after radical prostatectomy. hsa-miR-130b and RNU6-2 showed no significantly different expression between the matched malignant and non-malignant tissue samples, whereas hsa-miR-16 was significantly underexpressed in malignant tissue. Softwares geNorm and Normfinder predicted hsamiR-130b and the geometric mean of hsa-miR-130b and RNU6-2 as the most stable reference genes. Normalization of the four miRNAs hsa-miR-96, hsamiR-125b, hsa-miR-205, and hsa-miR-375, which were previously shown to be regulated, shows that normalization to hsa-mir-16 can lead to biased results. We recommend using hsa-miR-130b or the geometric mean of hsa-miR-130b and small RNA RNU6-2 for normalization in miRNA expression studies of prostate cancer.
APIS – A Miniaturized Robot for Precision Assembly with Low-Cost Piezoelectric Motors
Gunnar Borchert,Arne Burisch,Annika Raatz 한국정밀공학회 2011 International Journal of Precision Engineering and Vol. No.
This paper deals with the requirements for and the realization of a miniaturized parallel kinematic robot named APIS, which is driven by low-cost piezoelectric motors and thus designed for low-cost applications. After the need and potentials of this concept have been clarified, a detailed description of the development process is given. In doing so, kinematic aspects as well as the driving concept and the robot control, including the development of a suitable motor control with power stage,are described. In addition to the first functional prototype some performance measurements are presented, which show that the robot is able to obtain a repeatability of less than 34 μm. To provide better results, possibilities for future developments are identified, including optimized sensor feedback and motion control.
Eva-Maria Fick,Alexander Katalinic,Annika Waldmann 대한암학회 2015 Cancer Research and Treatment Vol.47 No.4
Purpose The aim of this cross-sectional health care study (use of bisphosphonates in primary tumorsof the mammae, EBisMa) is to determine how often bisphosphonate medication is used inpatients with non-metastatic primary breast cancer treatment, but who do not suffer fromosteoporosis. Furthermore, we describe patients’ characteristics and the most frequentlyused type of bisphosphonate in adjuvant therapy. Materials and MethodsThe study population included primary breast cancer patients of four breast centers in northernGermany. Data on bisphosphonate therapy were collected by use of patient questionnaires;clinical data were extracted from the registers. Patients with and without prescribedbisphosphonate adjuvant treatment were tested for statistically significant differencesregarding their characteristics. ResultsFour hundred seventy-four of 663 contacted patients participated in the study. Thirty-nineout of 474 patients (9.6%) were on adjuvant bisphosphonate therapy. Zoledronic acid wasthe most frequently reported bisphosphonate used for prevention of bone metastases. Comparedto patients who did not report bisphosphonate medication, women who did reportbisphosphonate therapy had a significantly higher advanced tumor stage (p < 0.001). Boththe T2-T4 stage and N+ stage remained significant predictors in multivariate-adjustedregression models. ConclusionBisphosphonates are rarely used in the adjuvant treatment of primary breast cancer. Patients with advanced tumor stage were more likely to use bisphosphonates in the adjuvanttreatment of primary breast cancer. Further research is needed to identify patientswho may benefit most from adjuvant bisphosphonate treatment.