Association of sericin and swimming on the phenotype, motor plate, and functionality of the denervated plantar muscle of Wistar rats

André Junior Santana,Jean Carlos Debastiani,Regina Inês Kunz,Pamela Buratti,Rose Meire Costa Brancalhão,Lucinéia de Fátima Chasko Ribeiro,Márcia Miranda Torrejais,Gladson Ricardo Flor Bertolini 한국운동재활학회 2018 JER Vol.14 No.1

Physical exercise may help maintain muscle properties and functional improvement after peripheral nerve lesion, which may be enhanced by using biocompatible substances, such as sericin. The aim of this study was analyse the effect of sericin associated with swimming exercise on the phenotype, innervation, and functionality of the plantar muscle of Wistar rats. Forty randomly divided adult rats were used in five groups of eight animals: control, injury, sericin, exercise, exercise and sericin. The application of sericin was done on the spot, 100 μL, shortly after nerve compression. Three days after sciatic nerve compression, the swimming and swimming and sericin groups were submitted to physical swimming exercise for 21 days. Afterwards, the animals were euthanised and the plantar muscle was dissected and submitted to histochemical and histoenzymological techniques. The grip strength test did not show alterations in muscular functionality, and the control presented greater muscle mass in relation to the other groups, the same did not occur for muscle length. Polymorphic neuromuscular junctions were detected in the groups, although without significant morphometric alterations of the area, major and minor diameters. The percentage of type I fibres was lower in the lesion group and there was no difference in fibres IIa and IIb between groups. The area of fibres I, IIa and IIb remained constant between groups. Sericin biopolymer combined with swimming exercise did not affect plantar muscle function, submitted to experimental axonotmosis, whose contractile properties were altered by nerve injury.

Optimization of steel-concrete composite beams considering cost and environmental impact

Andréia Fátima Tormen,Zacarias Martin Chamberlain Pravia,Fernando Busato Ramires,Moacir Kripka 국제구조공학회 2020 Steel and Composite Structures, An International J Vol.34 No.3

In the optimized structure sizing, the optimization methods are inserted in this context in order to obtain satisfactory solutions, which can provide more economical structures, besides allowing the consideration of the factors related to the environmental impacts in the structural design. This work proposes a mathematical model for the optimization of steel-concrete composite beams aiming to minimize the monetary cost and the environmental impact, using the Harmonic Search optimization method. Discrete variables were the dimensions of the steel profiles and the thickness of the collaborating slab of the composite steel-concrete beam. The proposed model was implemented in Fortran programming language and based on improvements in the structure of the optimization method proposed by Medeiros and Kripka (2017). To prove the effectiveness and applicability of the model, as well as the Harmonic Search method, analyzes were performed with different configurations of steel-concrete composite beams, in order to provide guidelines that make the use of these systems more streamlined. In general, the Harmonic Search optimization method has proved to be efficient in the search for the optimized solutions, as well as important considerations on the optimization of the monetary and environmental costs of steel-concrete composite beams were obtained from the developed examples.

The Pectate Lyase Encoded by the pecCl1 Gene Is an Important Determinant for the Aggressiveness of Colletotrichum lindemuthianum

Andréia Cnossen-Fassoni,Denise Mara Soares Bazzolli,Sérgio Hermínio Brommonschenkel,Elza Fernandes de Araújo,Marisa Vieira de Queiroz 한국미생물학회 2013 The journal of microbiology Vol.51 No.4

Colletotrichum lindemuthianum is the causal agent of anthracnose in the common bean, and the genes that encode its cell-wall-degrading enzymes are crucial for the development of the disease. Pectinases are the most important group of cell wall-degrading enzymes produced by phytopathogenic fungi. The pecC1l gene, which encodes a pectate lyase in C. lindemuthianum, was isolated and characterized. Possible cis-regulatory elements and transcription factor binding sites that may be involved in the regulation of genetic expression were detected in the promoter region of the gene. pecCl1 is represented by a single copy in the genome of C. lindemuthianum, though in silico analyses of the genomes of Colletotrichum graminicola and Colletotrichum higginsianum suggest that the genome of C. lindemuthianum includes other genes that encode pectate lyases. Phylogenetic analysis detected two groups that clustered based on different members of the pectate lyase family. Analysis of the differential expression of pecCl1 during different stages of infection showed a significant increase in pecCl1 expression five days after infection, at the onset of the necrotrophic phase. The split-maker technique proved to be an efficient method for inactivation of the pecCl1 gene, which allowed functional study of a mutant with a site-specific integration. Though gene inactivation did not result in complete loss of pectate lyase activity, the symptoms of anthracnose were reduced. Analysis of pectate lyases might not only contribute to the understanding of anthracnose in the common bean but might also lead to the discovery of an additional target for controlling anthracnose.

Evaluation of Women with Myofascial Abdominal Syndrome Based on Traditional Chinese Medicine

Andréia Mitidieri,Maria Beatriz Gurian,Ana Paula Silva,Kalil Tawasha,Omero Poli-Neto,Antônio Nogueira,Francisco Reis,Júlio Rosa-e-Silva 대한약침학회 2015 Journal of pharmacopuncture Vol.18 No.4

Objectives: This study used semiology based on traditionalChinese medicine (TCM) to investigate vital energy(Qi) behavior in women with abdominal myofascialpain syndrome (AMPS). Methods: Fifty women diagnosed with chronic pelvicpain (CPP) secondary to AMPS were evaluated by usinga questionnaire based on the theories of “yin-yang,”“zang-fu”, and “five elements”. We assessed the followingaspects of the illness: symptomatology; specific locationof myofascial trigger points (MTrPs); onset, cause, durationand frequency of symptoms; and patient and familyhistory. The patients tongues, lips, skin colors, and tonesof speech were examined. Patients were questioned onvarious aspects related to breathing, sweating, sleepquality, emotions, and preferences related to color, food,flavors, and weather or seasons. Thirst, gastrointestinaldysfunction, excreta (feces and urine), menstrual cycle,the five senses, and characteristic pain symptoms relatedto headache, musculoskeletal pain, abdomen, andchest were also investigated. Results: Patients were between 22 and 56 years old,and most were married (78%), possessed a elementaryschool (66%), and had one or two children (76%). Themean body mass index and body fat were 26.86 kg/cm2(range: 17.7 — 39.0) and 32.4% (range: 10.7 — 45.7), respectively. A large majority of women (96%) exhibitedalterations in the kidney meridian, and 98% hadan altered gallbladder meridian. We observed majorchanges in the kidney and the gallbladder Qi meridiansin 76% and 62% of patients, respectively. Five of thetwelve meridians analyzed exhibited Qi patterns similarto pelvic innervation Qi and meridians, indicatingthat the paths of some of these meridians were directlyrelated to innervation of the pelvic floor and abdominalregion. Conclusion: The women in this study showed changesin the behavior of the energy meridians, and the pathsof some of the meridians were directly related to innervationof the pelvic floor and abdominal region.

Novel and Highly Diverse Fungal Endophytes in Soybean Revealed by the Consortium of Two Different Techniques

Tiago de Souza Leite,Andréia Cnossen-Fassoni,Olinto Liparini Pereira,Eduardo Seiti Gomide Mizubuti,Elza Fernandes de Araújo,Marisa Vieira de Queiroz 한국미생물학회 2013 The journal of microbiology Vol.51 No.1

Fungal endophytes were isolated from the leaves of soybean cultivars in Brazil using two different isolation techniques – fragment plating and the innovative dilution-to-extinction culturing – to increase the species richness, frequency of isolates and diversity. A total of 241 morphospecies were obtained corresponding to 62 taxa that were identified by analysis of the internal transcribed spacer (ITS) of the ribosomal DNA (rDNA). The Phylum Ascomycota predominated, representing 99% and 95.2% of isolates in the Monsoy and Conquista cultivars, respectively, whereas the Phylum Basidiomycota represented 1% and 4.8% of isolates, respectively. The genera Ampelomyces, Annulohypoxylon, Guignardia, Leptospora, Magnaporthe, Ophiognomonia, Paraconiothyrium, Phaeosphaeriopsis, Rhodotorula, Sporobolomyces, and Xylaria for the first time were isolated from soybean; this suggests that soybean harbours novel and highly diverse fungi. The yeasts genera Rhodotorula and Sporobolomyces (subphylum Pucciniomycotina) represent the Phylum Basidiomycota. The species richness was greater when both isolation techniques were used. The diversity of fungal endophytes was similar in both cultivars when the same isolation technique was used except for Hill’s index, N1. The use of ITS region sequences allowed the isolates to be grouped according to Order, Class and Phylum. Ampelomyces, Chaetomium, and Phoma glomerata are endophytic species that may play potential roles in the biological control of soybean pathogens. This study is one of the first to apply extinction-culturing to isolate fungal endophytes in plant leaves, thus contributing to the development and improvement of this technique for future studies.

Evaluation of Purple Onion (Allium cepa L.) Extract as a Natural Corrosion Inhibitor for Carbon Steel in Acidic Media

Gustavo Tomas Galo,Andréia de A. Morandim‑Giannetti,Fernando Cotting,Idalina Vieira Aoki,Isabella Pacifico Aquino 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.9

The capacity of the Allium cepa L. extract from peel (ACP) and bulb (ACB) as a natural corrosion inhibitor for carbon steelwas evaluated. The corrosion characterization was performed by a gravimetric test, showing an inhibitor efficiency of 60%(ACP) and 67% (ACB). Electrochemical tests indicated higher corrosion resistance for carbon steel in the presence of theextracts, and the SEM images of steel samples confirm the results. These extracts were analyzed by the DPPH (2,2-diphenyl-1-picrylhydrazyl) method, confirming the antioxidant activity. The chemical characterization of the extracts by FTIR measurementsrevealed the presence of phenolic compounds with antioxidant capacity.

Gynecologic cancer in Brazil and the law of sixty days

Eduardo Paulino,Eduardo Paulino,Andréia Cristina de Melo,Angélica Nogueira-Rodrigues,Luiz Claudio Santos Thuler 대한부인종양학회 2018 Journal of Gynecologic Oncology Vol.29 No.3

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Morphological traits of stem to indirect selection of resistance to lodging in Avena sativa L

Silveira Diógenes Cecchin,Basso Simone Meredith Scheffer,Ebone Luciano Antônio,Caverzan Andréia,Machado Juliana Medianeira,Schaeffer Afonso Henrique,Folchini Jessica Aneris,Lângaro Nadia Canali 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.1

The runout of newly cultivated areas in the world without recurring to the deforestation, demand a necessity to improve yield to sustain a growth population which demands each time more food. To avoid that is necessary to solve the main issues which cause crop productivity reduction, such as lodging which causes yield reduction in many major crops such as wheat, barley, rice and oat. In this sense, a study with eight cultivars evaluated morphological traits of the stem as crown diameter, internal and external diameter of the frst four basal internodes, the length of internodes, peduncle and panicle, plant height, and also lodging resistance factor (cLr), green and dry panicle matter. The diameter of the crown does not show diferences among the cultivars, the morphological characters which showed correlation and relative contribution with cLr were the internal and external internodes diameters, showing their capacity to be one of morphological traits of the stem to an indirect selection of resistance to lodging in Avena sativa L. The length did not show a good correlation with lodging, probably due to the fact that this trait was the focus of breeders for many years, reaching the plateau, demanding focus in other traits to avoid lodging such as interna and external diameter of internodes. The cultivar URS Brava and IPR Afrodite demonstrated to be a source of lodging resistance in oat.

Survival of women with ovarian carcinomas and borderline tumors is not affected by estrogen and progesterone receptor status

Luis Felipe Sallum,Luis Otavio Sarian,Liliana Lucci De Angelo Andrade,José Vassallo,Fernando Augusto Soares,Glauce Aparecida Pinto,Patrícia Andréia Ferreira,Sophie Derchain 대한부인종양학회 2013 Journal of Gynecologic Oncology Vol.24 No.2

Objective: To examine the patterns of estrogen receptor (ER) and progesterone receptor (PR) expression in borderline ovarian tumors (BOTs) and ovarian carcinomas. We also assessed the disease-free survival (DFS) and overall survival (OS) in women with ovarian carcinoma, in relation to ER and/or PR expression. Methods: We examined ER/PR expression in 38 BOTs and 172 ovarian carcinomas removed from patients treated at the State University of Campinas-UNICAMP (Brazil), from 1993 to 2008 and followed for up to 60 months using tissue microarray-based immunohistochemistry. Results: Twenty-eight (73.7%) mucinous and 10 (26.3%) serous BOTs were included. Ovarian carcinomas consisted mainly of 79 (46.0%) serous, 44 (25.5%) mucinous, 17 (9.8%) endometrioid, 10 (5.8%) clear-cell types. There was no significant difference of the ER/PR expression between BOT and ovarian carcinoma (p=0.55 for ER alone, 0.90 for PR alone, and 0.12 for combined expression). The level of ER/PR expression in BOTs was significantly higher in serous than in mucinous tumors (p<0.01). In carcinomas, ER/PR was higher in serous tumors than in mucinous (p<0.01) and clear cell tumors (p=0.02), and higher in endometrioid tumors than in mucinous tumors (p<0.01). DFS was affected neither by the clinical characteristics nor by combined steroid receptor status. OS was found to be significantly worse (p<0.01) only in women with stages II-IV tumors and those with residual disease after surgery (p<0.01). Conclusion: Overall, serous and endometrioid tumors were predominantly ER/PR positive, whereas mucinous and clear-cell tumors were preponderantly ER/PR negative. DFS and OS were not affected by ER/PR expression.

Effect of the Auricular Acupoint Associated with Physical Exercise in Elderly People: A Randomized Clinical Test

Roge´ria Gonc¸alves de Carvalho Fonseca,Andre´ia Maria Silva,Luiza Faria Teixeira,Vale´ria Regina Silva,Luciana Maria dos Reis,Adriana Teresa Silva Santos 사단법인약침학회 2018 Journal of Acupuncture & Meridian Studies Vol.11 No.4

Objective: To analyze the effect of auricular acupoint associated with physical exercise on balance, mobility, and fear of falling in the elderly. Method: The study is characterized as a clinical, controlled, and randomized trial with 22 elderly people divided into two groups: kinesiotherapy group (n Z 11) and kinesiotherapy/auriculotherapy group (n Z 11). The instruments used for evaluation were Falls Efficacy Scale International; Berg Balance Scale, and Timed up and Go Test. The intervention was performed with frequency 2Â/week for 8 weeks. In the kinesiotherapy/auriculotherapy group, in addition to kinesiotherapy, auriculotherapy was applied in specific acupoints. The ShapiroeWilk test was used to determine the normality of the data, and for comparison, analysis of variance was used for repeated measures of two factors.