Rapid detection of avian influenza A virus by immunochromatographic test using a novel fluorescent dye

Yeo, Seon-Ju,Cuc, Bui Thi,Kim, Soon-Ai,Kim, Do Thi Hoang,Bao, Duong Tuan,Tien, Trinh Thi Thuy,Anh, Nguyen Thi Viet,Choi, Do-Young,Chong, Chom-Kyu,Kim, Hak Sung,Park, Hyun Elsevier Applied Science 2017 Biosensors & Bioelectronics Vol. No.

<P><B>Abstract</B></P> <P>Sensitive and rapid diagnostic systems for avian influenza (AI) virus are required to screen large numbers of samples during a disease outbreak and to prevent the spread of infection. In this study, we employed a novel fluorescent dye for the rapid and sensitive recognition of AI virus. The styrylpyridine phosphor derivative was synthesized by adding allyl bromide as a stable linker and covalently immobilizing it on latex beads with antibodies generating the unique Red dye 53-based fluorescent probe. The performance of the innovative rapid fluorescent immnunochromatographic test (FICT) employing Red dye 53 in detecting the AI virus (A/H5N3) was 4-fold and 16-fold higher than that of Europium-based FICT and the rapid diagnostic test (RDT), respectively. In clinical studies, the presence of human nasopharyngeal specimens did not alter the performance of Red dye 53-linked FICT for the detection of H7N1 virus. Furthermore, in influenza A virus-infected human nasopharyngeal specimens, the sensitivity of the Red dye 53-based assay and RDT was 88.89% (8/9) and 55.56% (5/9) relative to rRT-PCR, respectively. The photostability of Red dye 53 was higher than that of fluorescein isothiocyanate (FITC), showing a stronger fluorescent signal persisting up to 8min under UV. The Red dye 53 could therefore be a potential probe for rapid fluorescent diagnostic systems that can recognize AI virus in clinical specimens.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Expression of the RERG Gene is Gender-Dependent in Hepatocellular Carcinoma and Regulated by Histone Deacetyltransferases

Ai-Guo Wang,Wan Fang,Ying-Hao Han,Sang-Mi Cho,Jong Young Choi,Kee Ho Lee,Wook Hwan Kim,Jin Man Kim,Moon Gi Park,Dae-Yeul Yu,Nam-Soon Kim,이동석 대한의학회 2006 Journal of Korean medical science Vol.21 No.5

측뇌실에서 고농도 Na^+에 대한 고농도 Ca^++의 심혈관계 영향

최애경,김경순,안동국,박윤엽,박재식 慶北大學校 醫科大學 1995 慶北醫大誌 Vol.36 No.2

목적 : 고농도의 NaCl 용액을 측뇌실에 주입하여 동맥압 및 심박수의 변화를 관찰하고, 또한 Ca^++을 전처치한 후 NaCl 용액을 주입하여 역시 동맥압과 심박수를 관찰하여 이들의 상호 관계를 알아보았다. 대상 및 방법 : 체중 340∼480g의 Wistar 숫쥐를 마취한 후 뇌정위 고정 장치에 쥐를 고정하고 측뇌실에 관을 삽입하였다. 수술후 5일이 경과한 다음 대회동맥에 관을 삽입한 후 깨어있는 상태에서 측뇌실에 용액을 2㎕/min의 속도로 주입하였다. 출혈 실험에서 출혈은 빠른 시간내에 1.5㎖/100g BW으로 하였다. 결과 : 960mOsm/l NaCl 용액 주입에 의해 동맥압은 증가하고 심박수는 감소하였다. Ca^++을 전처치한 후 NaCl을 주입한 경우에도 동맥압은 증가하고 심박수는 감소하였지만 NaCl 단독으로 주입한 경우보다는 동맥압의 증가가 둔화되었다. NaCl 용액 주입후 출혈을 시키면 대조군에 비해 동맥압 감소가 적었으며 심박수의 증가는 컸다. 그러나 Ca^++을 전처치한 후 NaCl을 주입하여 출혈시킨 경우는 동맥압 및 심박수 변화가 대조군과 비슷하였다. 출혈시 압감수성 반사는 NaCl 주입군에서 가장 큰 경향을 보였다. 한편 혈장 renin은 NaCl 주입군에서 대조군보다 낮았으며, Ca^++을 전처치한 후 NaCl 주입한 군에서는 대조군보다는 낮았지만 NaCl을 단독으로 투여한 군보다는 높았다. 혈장 AVP는 NaCl 주입군이 대조군보다 높았다. 결론 : 측뇌실내 고농도의 Na^+은 교감신경 활성도를 촉진시켜 동맥압을 상승시키며, Ca^++은 교감신경을 억제하여 Na^+와 반대되는 작용을 나타내었다. The contribution of the central interaction between calcium and sodium ions to hemodynamic regulation was assessed in Wistar rats. The effect of a high calcium solution (Ca^++, 130 ㎎/㎗, infused into the cerebral ventricle (i.c.v.) on hemodynamic responses induced by i.c.v. high sodium (Na^+, 960mOsm/1, 20㎕) was studied. And also the effect of hemorrhage on the cardiovascular response was investigated. Under pentobarbital anesthesia, stainless steel cannula (22 gauge) was implanted stereotaxically in a lateral cerebral ventricle according to Paxinos' brain atlas. After a 5-day recovery period, the femoral artery was catheterized, under thiopental anesthesia, with PE50 tubing filled with heparinized saline. Upon recovery, high NaCl solution was infused into the lateral ventricle through the cannula at a rate of 2㎕/min for 10 min. After the mean arterial pressure and heart rate had returned to their baseline values, high Ca^++, followed shortly by a second dose of high Na^+, was administered centrally. Acute hemorrhage (1.5㎖/100) was induced before and after infusion of NaCl solution and after infusion of Ca^++ pretreated NaCl solution. High i.c.v. Na^+ increased the MAP, but decreased the HR. Prior treatment with i.c.v. high Ca^++ reduced the pressor reponse to high Na^+ i.c.v. Treatment with high NaCl solution resulted in a less decrease in MAP caused by hemorrhage. After pretreatment with high Ca^++, however, MAP de creased to the same level as in the control group in response to hemorrhage. Plasma renin activity was lowered and arginine vasopressin elevated in high Na^+ group. These results suggest that high Na^+ in the lateral ventricle produces an increase in MAP by increasing the sympathetic nervous system activity. In this respect, high Na^+ might be helpful in recovering from blood loss. Also, central Ca^++ antagonizes the pressor response to central Na^+ by inhibiting the sympathetic nervous system activity. Central Ca^++, therefore, may have importance in the control of hypertension.

Expression of the RERG Gene is Gender-Dependent in Hepatocellular Carcinoma and Regulated by Histone Deacetyltransferases

Wang, Ai-Guo,Fang, Wan,Han, Ying-Hao,Cho, Sang-Mi,Choi, Jong Young,Lee, Kee Ho,Kim, Wook Hwan,Kim, Jin Man,Park, Moon Gi,Yu, Dae-Yeul,Kim, Nam-Soon,Lee, Dong-Seok The Korean Academy of Medical Sciences 2006 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.21 No.5

<P>Ras-related, estrogen-regulated, and growth-inhibitory gene (RERG) is a novel gene that was first reported in breast cancer. However, the functions of RERG are largely unknown in other tumor types. In this study, RERG expression was analyzed in hepatocellular carcinomas of human patients using reverse transcriptase PCR analysis. In addition, the possible regulation of RERG expression by histone deacetyltransferases (HDACs) was studied in several cell lines. Interestingly, the expression of RERG gene was increased in hepatocellular carcinoma (HCC) of male patients (57.9%) but decreased in HCC of females (87.5%) comparison with paired peri-tumoral tissues. Moreover, RERG gene expression was increased in murine hepatoma Hepa1-6 cells, human breast tumor MDA-MB-231 cells, and mouse normal fibroblast NIH3T3 cells after treated by HDAC inhibitor, trichostatin A. Our results suggest that RERG may function in a gender-dependent manner in hepatic tumorigenesis and that the expression of this gene may be regulated by an HDAC-related signaling pathway.</P>

여대생 기숙사 식단의 필순 아미노산 산출시와 분석시의 비교조사

박명순(Myoung Soon Park),김성애(Seong Ai Kim) 충남대학교 생활과학연구소 1988 忠南生活科學硏究誌 Vol.1 No.-

초등학교 급식유형에 따른 영양사의 직무 만족도

김성애,박명순,한장일 한국식생활문화학회 1999 韓國食生活文化學會誌 Vol.14 No.3

This study was to investigate the factors which influence foodservice dietitians' job satisfaction and dissatisfaction by the elementary school foodservice type using questionnaires. The results of the study are as followings : 1) Foodservice types of the subjects were urban 49(23.2%), country 141(66.8%) and island 21(10.0%). 79.6% of the urban type were operated by the conventional, independent management system, where 38.3% of the country and 52.4% of the island type were operated by conventional, joint management system. 2) Urban type dietitians showed significantly lower satisfaction on their salary & promotion(1.5) compare to country & island types(p<0.05). Island type dietitians showed significantly lower satisfaction on the student's health improving(2.4) compare to country & urban types(p<0.05). 3) Dietitians of urban type were dissatisfied more 'personnel management(3.2)' than dietitians of other two types. Dietitians of country type and island type were dissatisfied more 'extra work load(2.6)' than dietitians of urban type. 4) Work load was lots orderly island, country and urban types. Working attitudes were the most positive at dietitians of urban type and the most negative at dietitians of island type. 5) Job satisfaction and dissatisfaction of dietitians were correlated with number of meals, number of school transfer, cook license, additional job and school foodservice location type.

좋은 식단제를 위한 대전지역 요식업소 경영주들의 의식구조 조사

김성애,박명순,한장일 한국식생활문화학회 1999 韓國食生活文化學會誌 Vol.14 No.2

This study was conducted to investigate the sanitation status and food waste state from 220 restaurant owners in Taejon area by questionnaires. As a result, the restaurant owners had proper knowledges on the purposes of the good menu system which is to prove public sanitation and to reduce food waste by changing present food service style. According to the restaurant management characteristics study, the subjects recognized highly for the importance of cleanliness(4.6), taste(4.5) where diversity of portion size(3.9), attractiveness(4.0) were low. 86.3% of the subjects mentioned great reductions in food waste after practicing good menu system. Also the subjects recognized the necessity for further researches on this field. After practicing good menu system in restaurant, their food waste was reduced.

Treadmill exercise alleviates prenatal noise stressinduced impairment of spatial learning ability through enhancing hippocampal neurogenesis in rat pups

Tae-Woon Kim,Mal-Soon Shin,Joon-Ki Park,Mi-Ai Shin,Hee-Hyuk Lee,Sam-Jun Lee 한국운동재활학회 2013 JER Vol.9 No.5

Physician Compliance with Tube Feeding Protocol Improves Nutritional and Clinical Outcomes in Acute Lung Injury Patients

나성원,이호선,고신옥,Ai Soon Park,한아름 대한중환자의학회 2010 Acute and Critical Care Vol.25 No.3

Background: Nutrition delivery is frequently interrupted or delayed by physicians’ ordering patterns. We conducted this study to investigate the effect of physician compliance with tube feeding (TF) protocol on the nutritional and clinical outcomes in acute lung injury (ALI) patients. Methods: After implementing a TF protocol, 71 ALI patients with mechanical ventilation (MV) for ≥ 7 days were observed. A dietician assessed the nutritional status of the patients and established individualized nutrition plans according to the protocol. If the physicians followed the dietician’s recommendation within 48 hours, the patients were classified under the compliant group (Group 1). Results: Forty patients (56.3%) were classified into Group 1. Prealbumin was comparable in both groups at ICU admission but higher in Group 1 at the time of discharge from the ICU (228 ± 81 vs 157 ± 77 mg/dl, p =0.025). Nitrogen balance was only improved in Group 1. The time to reach calorie goal was shorter and non-feeding days were reduced in Group 1. The proportion of parenteral nutrition to nutritional support days was lower and delivered calories on the 4th and 7th day of TF were higher in Group 1 (p < 0.001). ICU mortality/stay and hospital mortality failed to show differences but hospital stay was prolonged in the noncompliant group (Group 2) (p =0.023). Arterial oxygen tension and PaO2/FiO2 were maintained during the 1st week of ICU stay in Group 1 but were decreased in Group 2. Conclusions: Physicians’ compliance with the TF protocol contributed to the likelihood of nutritional improvement and a shorter hospital stay in ALI patients with prolonged MV.

各種 疾患의 Gamma Globulin値의 比較觀察

金元徹,朴貞愛,徐永順,利鍾德,尹德鎭 대한의학협회 1966 대한의학협회지 Vol.9 No.9