계면활성제/금속이온 혼합용액의 한외여과막에 의한 카드뮴 구리 및 아연이온의 제거

안순철,이광래 江原大學校 附設 環境硏究所 1999 環境硏究 Vol.16 No.-

계면활성제/금속이온 혼합물의 셀룰로즈 아세테이트막에 의한 금속이온의 배제율, 계면활성제의 배제율, 농도분극에 의한 투과저항 등은 계면활성제의 농도에 따라 증가하였으며, 용액의 투과도는 감소하였다. 계면활성제만이 용해되어 있는 용액에서, 계면활성제 SDS(M.W. 288) 와 SLS(M.W. 420)의 분자량이 세룰로즈 아세테이트 막의 분획분자량(MWCO 1,000) 보다 작음에소 불구하고, SDS의 17%와 SLS의 66%가 막에 의해 배제되었다. 그러나, 계면활성제/금속이온(Cd^(++), Cu^(++), Zn^(++)) 혼합물의 경우, SDS는 83%~88%, SLS는 약 94%가 배제되었다. 카드뮴이온(Cd^(++))은 100%, 구리이온은 75%, 아연은 79%의 배제율을 나타내었다. 금속이온/SDS 혼압용액 ((Cd^(++), Cu^(++), Zn^(++)) 의 투과도는 순수한 물의 투과도 (10.9×10^(-3)㎖/min · ㎠ · atm)보다 약 36%가 감소하였다. 금속이온/SDS 혼합용액((Cd^(++), Cu^(++), Zn^(++)) 의 농도분극에 의한 투과저항은 막의 고유저항 ((Rm=6.24×10^(13) m^(-1))의 약 55%에 이르렀다. 용질 (계면활성제)에 의한 막의 막힘(fouling)이 발생하지 않았으며, 막의 막힘현상에 의한 투과 저항은 없었다. 계면활성제/금속이온 혼합물의 농도분극 현상에 의한 삼투압(Δπ)은 무시할 만 하였다. Rejection rate of surfactant (SDS, SLS), rejection rate of metal ions, and permeation resistance were increased, and permeation flux due to concentration polarization was declined with increase in concentration of surfactant. Although the molecular weights of surfactant SDS(M.W. 288) and SLS(M.W. 420) are smaller than the molecular weigth cut-off(MWCO 1,000) of cellulose acetate membrane used in this study. 17% of SDS and 66% of SLS were rejected bly cellulose acetate membrane in case of the aqueous solution of surfactant only. However, 83%~88% of SDS and 94% of SLS in surfactant/metal ions mixture were rejected. The rejection rates of cadmiu,. copper, and zinc ion were 100%, 75%, and 79%, respectively. Permeation flux of 24mM SDS solution/metal ions was decreased by 36% of pure water permeation rate(10.9×10^(-3)㎖/min · ㎠ · atm). Permeation resistance due to concentration polarization increased upto 55% of intrinsic membrane resistance(Rm=6.24×10^(13) m^(-1)). There was no resistance to permeation rate by membrane fouling, and osmotic pressure due to the concentration polarization was negligible.

셀룰로즈 아세테이트 분리막에 대한 계면활성제의 투과거동

안순철,송근호,이광래 江原大學校 附設 環境硏究所 1999 環境硏究 Vol.16 No.-

계면활성제 SDS(M.W. 288)와 SLS(M.W. 420)의 분자량이 셀룰로즈 아세테이트 막의 분획분자량 (MWCO 1,000) 보다 작음에도 불구하고, SDS의 17%와 SLS의 66%가 막에 의해 배제되었다. SLS의 배제율이 SDS의 배제율보다 더 높은 것은 소수기 사슬이 길고 분자량이 큰것에 기인한다. 막오염에 의한 투과저항은 나타나지 않았으며, 농도분극현상에 의한 삼투압은 무시할만 하였다. 계면활성제 SDS와 SLS의 막에 의한 계면활성제의 배제율, 농도분극에 의한 투과저항 등은 계면활성제의 농도에 따라 증가하였으며, 용액의 투과도는 감소하였다. 투과플럭스는 SDS용액의 농도가 24Mm일 때, 막의 고유저항(Rm=6.24×10^(13) m^(1))의 47%에 이르렀다. SLS의 경우 165%에 달했다. The molecular weights of surfactant SDS(M.W. 288) and SLS(M.W. 420) are smaller than the molecular weight cut-off (MWCO 1,000) of cellulose acetate membrane used in this study. However, 17% of SDS and 66% of SLS were rejected by cellulose acetate membrane. The higher rejection rate of SLS than that of SDS is due to the longer hydrophobic chain and greater molecular weight. There was no resistance to permeation rate by membrane fouling, and osmotic pressure due to the concentration polarization was negligible. Rejection rate of surfactant (SDS, SLS) and permeation resistance were increased, and permeation flux due to concentration polarization was declined with increase in concentration of surfactant. Permeation flux of 24mM SDS solution was decreased by 32% as compared to pure water permeation rate (10.9×10^(-3)㎖/min · ㎠ · atm), and decreased by 62% in case of 24 24mM SDS. Permeation resistance due to concentration polarization of 24mM SDS increased upto 47% of intrinsic membrane resistance(Rm=6.24×10^(13) m^(1)), and increased upto 165% in case of 24mM SDS.

수학에 대한 태도와 불안에 관한 연구

박순철,고안상 朝鮮大學校 師範大學 附設 敎科敎育硏究所 2005 敎科敎育硏究 Vol.26 No.1

Looking into the number of students taking "Soo-neung" test or the results from the enquete for the purpose of organizing the option-based curriculum after the 7th curriculum began, we can see it becomes clearer that the number of students applying for humanity course has largely outnumbered the number of students applying for science course since the late 1990s. These phenomena must be big troubles for us looking forward to getting into the advanced country. So, there came the counterplans from government, college, and enterprise in the hope that the good students are willing to apply for science course. In order to find out why these phenomena of evasion from science course happens, this article deals with the attitude and anxiety of elementary, middle, and high school students about math. Consider the following: 1. How is the transition of elementary, middle, and high school students In the attitude about math? 2. How is the transition of elementary, middle, and high school students III math anxiety? 3. How are the attitudes about math related with math anxiety, and the researches about how to decrease math anxiety evolved? Analyzing enquete using attitude standard made by Aiken and Dreger(1963) and math anxiety standard (MAS) made by Fennema and Sherman(1976). we've found the conclusion is as follows: First, we should offer the positive learning experience to the students who have the negative attitude about math and try to help them lower the degree of the math anxiety. Secondly, there are plenty of room for making an effort in that the number of students who don't like math, as they grow older, increases, but the number of them is not that much. Thirdly, the math anxiety of the 2nd grade students in the middle school becomes more increased when compared in terms of a average of factors. But there was no meaningful difference over the schools. Finally, we have become aware that the math attitude is highly related with the math anxiety. And it is hoped that these studies of the attitude and anxiety about math would be well applied to the actual spots. schools.

변이주인 Trichoderma reesei YC-3156이 생산하는 Cellulase에 관한 연구

오두환,안순철,유주현,허남윤 연세대학교 산업기술연구소 1987 논문집 Vol.19 No.2

After the treatment 15 strains of Trichoderma sp. and 4 isolates with N-methyl-N´-nitro-nitrosoguanidine, T. reesei YC-3156 among the mutants showed the highest cellulase activity. The Avicelase of T. reesei YC-3156 was purified by 70% ammonium sulfate fractionation, dialysis, DEAE-Sepharose column chromatography and Sephadex G-100 Gel-filtration. With the result of the procedures, its purification degree was 13 folds, yield 9.3% and specific activity 0.9 unit/mg. The homogeneity and the molecular weights of the purified Avicelase were determined by SDS-polyacrylamide gel electrophoresis, it was electrophoretically homogeneous and showed the molecular weights of 46,000-48,000. The purified Avicelase was stable between pH 4.0 and pH 5.0 and it was inactivated rapidly above 60℃.

계면활성제의 분리막에 대한 투과거동

이광래,안순철,김기창 江原大學校 産業技術硏究所 1998 産業技術硏究 Vol.18 No.-

The molecular weights of surfactant SDS(M.W. 288) and SLS(M.W. 420) are smaller than the molecular weigh cur-off of cellulose acetate membrane used in this study. However, 20% of SDS and 67% of SLS were rejected by cellulose acetate membrane. The higher rejection rate of SLS than that of SDS is due to the longer hydrophobic chain and greater molecular weight. There was no resistance to permeation rate by membrane fouling. Most of permeation resistance was due to the concentration polarization. Permeation rate was declined with operating time and with increase in concentration of surfactant due to concentration polarization.

단무지 공장에서 발생한 질식 사고의 원인과 방사선학적 소견: 중례보고 및 가스분석 결과

박충기,김만구,김흥철,안범규,박만수,황우철,최철순,강익원 江原大學校 附設 環境硏究所 1994 環境硏究 Vol.11 No.-

목 적:단무지 공장에서 질식사고를 일으켰던 가스의 종류를 알아내고, 이러한 가스중독의 방서선학적 소견을 소개하기 위하여 본 연구를 하였다. 대상 및 방법:단무지 공장에서 발생한 질식 사고자 3예중 생존자 1예를 대상으로 단순흥부X-선검사와 CT 소견을 분석하였으며, 가스의 종류를 알아내기 위하여 단무지를 유리병에 넣어 발생된 가스를 가스크로마토그래픽을 이용하여 분석하였다. 결 과:단무지 공장에서 발생한 질식사고자중 생존한 1명의 방사선학적 검사에서 신속히 호전되는 폐경결(consolidation)을 볼 수 있었으며 이는 폐부종의 소견임을 알 수 있었다. 협기성 상태에서 단무지를 담은 유리병의 상층부 가스를 실험 분석한 결과 이산화탄소, 에칠알코올이 다량으로 검출되었으나 독성가스는 주로 황화수소이였다. 결 론:단무지 공장의 질식 사고자에서 폐방사선학적 소견은 폐경결을 보이는 폐부증으로서 이는 실험을 통해 단무지에서 발생되는 유독가스인 황화수소 때문임을 입증하였다. Purpose: To identify the main toxic gas released from salted radish in rice bran(Dan-M-Ji) and to introduce the radiological findings of the patient who was exposed to the gas. Materials and Methods: Chest radiographs and CT scans of one survivor among three men who were exposed to the gas from Dan-Mu-Ji were reviewed. Gas obtained from the closed bottle containing Dan-Mu-Ji was analized by using the gas chromatography. Results: The radiographlc examinations of the survivor were suggestive of pulmonary edema with it's rapidly improving consolidations in both lung. The headapace gas within the bottle containing Dan-Mu-Ji was mainly composed with carbon dioxide, ethyl alcohol and hydrogen sulfide, of which hydrogen sulfide was considered the main toxic gas released. Conclusion: Under the anaerobic condition, Dan-Mu-Ji released toxic hydrogen sulfide. Inhalation of hydrogen sulfide might produce non-cardiogenic pulmonary edema.

TiO₂미세분말 제조에 관한 연구

이광래,안순철,송근호,김종수 강원대학교 석재복합신소재제품연구센터 1998 석재연 논문집 Vol.3 No.-

역마이셀 기법에 의하여 TiO₂ 분말을 제조하였으며, TiO₂ 분말 제조에 미치는 공정변수들인 계면활성제의 종류 및 보조계면활성제가 생성입자의 입도분포에 미치는 영향을 규명하였다. 비이온계면활성제인 Span 80을 사용하였을 경우에는 입도분포가 unimodal형태였으나, 음이온계면활성제인 Aerosol-OT(AOT)를 사용하였을 경우, bimodal형태를 나타내었다. 에탄올(ethanol)을 cosurfactant로 첨가한 계로 부터 생성된 입자는 평균입경이 0.12㎛ 으로서 미세할 뿐아니라 입도분포도 매우 좁은 것을 알 수 있다. Titanium dioxide (TiO₂) fine particles were produced by the reverse micelle technique. The effects of process variables such as surfactant and cosurfactant on particle size distribution were investigated. The particle size distribution curve was unimodal for a nonionic surfactant (Span80), but was bimodal for an anionic surfactant (AOT). The addition of ethanol as a cosurfactant resulted in narrow particle size distribution and 0.12㎛ of smaller average particle diameter.

방선균 분리주가 생산하는 Phospholipase C 저해물질인 MT2617-2B의 분리 및 특성

고학룡,이현선,오원근,안순철,김보연,강대욱,민태익,안종석 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

방선균 분리주 MT2617-2의 배양액으로 부터 phospholipase C (PLC) 저해물질인 MT2617-2B를 n-butanol 추출 및 column chromatography 법을 이용하여 분리하였다. MT2617-2B는 IR ^13C- 및 ^1H-NMR 그리고 ESI-MS에 의한 구조분석 결과, 한 개의 hemiketal ring, polyhydroxyl 및polymethyl groups으로 구성되었으며 side chain으로 한 개씩의 malonate 및 guanidine group을 가지는 분자량 1057의 macrolide 화합물이었다. 따라서, MT2617-2B를 기존의 macrolide 항생제인 copiamycin 및 niphithricin A로 동정하였다. 한편, MT2617-2B는 methanol 용액에서 실온에서 방치하였을 때 도일한 분자량을 가진 두 개의 이성질체를 생성하였다. PLC γ1과 -β1에 대해 각각 25 및 50㎍/㎖의 IC_50 값을 가지며, Staphylococcus aureus 와 Candida albicans에 대해서는 항균활성을 나타내지만 Escherichia coli에는 나타내지 않았다. A phospholipase C (PLC) inhibitor (MT2617-2B) was isolated from the culture broth of actionmycetes isolate MT2617-2 by the extraction with n-butanol and column chromatographic techniques. The molecular weight of the inhibitor was 1057, by the spectroscopic analyses of IR ^13C- and ^1H-NMR and ESI-MS. The chemical structure of MT2617-2B was found to be a macrolide compound consisted of a hemiketal ring, polyhydroxyl and polymethyl groups, which had a malonate and guanidine group as its side chain. MT2617-2B produced its two isomers having the same molecular weight by standing in methanol solution at room temperature. Therefore, MT2617-2B was identified as copiamycin and niphithricin A, macrolide antibiotics. The values of IC_50 against PLC γ1 and PLC-β1 were 25 and 50㎍/㎖, respectively. MT2617-2B had antimicrobial activities against Staphylococcus aureus and Candida albicans, but not against Escherichia coli.

수술실 오염도감소를 위한 수술실공조시스템에 대한 연구

김천숙,박은정,강규식,김지은,안기량,권진형,유시현,주영철,권순정 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Background: The OR(operating room) should provide an optimum environment that is safe for the patient and the working personnel. The air ventilation system of OR has been studied to decrease the contamination of air. We investigated the flow and contamination of the air in OR at our hospital. Method: Cultures in OR were made and the number of bacteria and fungi were calculated. A two-dimensional model for the cross-section of an operation room is developed for the CFD (computational fluid dynamics) analysis. The characteristics of air flow in the empty operation room and in the occupied operation room are calculated by using a CFD program. Results: The current air ventilation system of our hospital does not deliver the clean air to the operating site efficiently in occupied OR. Conclusions: We suggested a new location of air ventilation system which improves air venting with little increase of the cost of equipment.

ABSTRACTS ; Posters : PURIFICATION OF MT6912B , INHIBITOR OF PHOSPHOLIPASE C AND PROTEIN KINASE C FROM ACTINOMYCETES

Soon Cheol Ahn,Bo Yeon Kim,Dae Oook Kang,Hyun Sun Lee,Joon Shick Rhee,Jong Seog Ahn 한국생화학분자생물학회 (구 한국생화학회) 1995 추계학술대회집 Vol.- No.-