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Adriana Filip,Doina Daicoviciu,Simona Clichici,Teodora Mocan,Adriana Muresan,Ion Dan Postescu 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.7
Solar ultraviolet radiation (UV) is the major cause of nonmelanoma skin cancer in humans. Photochemoprevention with natural products represents a simple but very effective strategy for the management of cutaneous neoplasia. We studied the photoprotective activity of Calluna vulgaris and red grape seed (Vitis vinifera L, Burgund Mare variety [BM]) extracts in vivo in an SKH-1 hairless mice skin model. Fifty 8-week-old female SKH-1 hairless mice were randomly divided into 5 groups (n = 10 each): controls, UVB-irradiated, C. vulgaris plus UVB–irradiated, BM plus UVB–irradiated, and epigallocatechin gallate (EGCG) plus UVB–irradiated. A dose of 4 mg/mouse per ㎠ of skin area for both extracts was topically applied to the mice 30 minutes before a single-dose (240 mJ/㎠) UVB exposure. EGCG dissolved in phosphate-buffered saline (pH 6.6; 0.067 M) was administered at 2 mg/mouse per ㎠. Glutathione peroxidase and catalase activities, reduced glutathione (GSH), malondialdehyde, nitric oxide, and caspase 3 activity were determined in skin homogenates 24 hours after irradiation. A single dose of UVB increased GSH levels and glutathione peroxidase activity in the exposed skin. C. vulgaris and BM pretreatment significantly decreased GSH formation and glutathione peroxidase activity (P < .001) and inhibited UVB-induced lipid peroxidation (P < .0001) and nitric oxide production (C. vulgaris: P < .06). Topical treatments with C. vulgaris and particularly BM extracts (P < .002) significantly reduced caspase 3 activity, indicating that the cells were protected against apoptosis. These results suggest that C. vulgaris and BM extracts might be chemopreventive candidates for reducing UV-induced risk for skin cancer.
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( Adriana M. Rangel-rodriguez ),( J. Adriana Sañudo-barajas ),( Nagamani Balagurusamy ),( Louise Wicker ),( Rosabel Velez-de-la-rocha ),( Juan Carlos Contreras-esquivel ) 한국키틴키토산학회 2018 한국키틴키토산학회지 Vol.23 No.2
In this work, the preparation and characterization of water-soluble polysaccharides from chitosan and Aloe vera was studied. The water-soluble polysaccharides were used to study polyelectrolyte complexes. The reaction time effect for chitosan hydrolysis by endo-chitosanase was studied at 40ºC and pH 5.00 to produce water soluble chitosan (WSCh). The physico-chemical characteristics of chitosan hydrolysates, water-soluble A. vera polysaccharides and polyelectrolyte complexes were determined. After 3 h of chitosan processing, a viscosity reduction of 90%, while only 2.3% of reducing sugars were released. A WSCh was recover by ultrafiltration (1 kDa) from chitosan hydrolysate after 12 h and was spray-dried with a yield of 9.7%. Cold-water extraction of A. vera mucilage from pulp gives a crude polysaccharide yield of 0.81 g/kg (wet basis) based on whole leaf weight. The water isolated mucilage is composed of a mixture of protein and mannan rich-polysaccharide. The results show WSCh’s association capacity with A. vera mucilage by electrostatic interaction.
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Grape Seed Extract Effects in Brain After Hypobaric Hypoxia
Adriana Muresan,Soimita Suciu,Doina Daicoviciu,Adriana Gabriela Filip,Simona Clichici 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.9
Hypoxia induces a wide range of deleterious effects at the cellular level due to an increased production of reactive oxygen species (ROS). Polyphenols from grape seeds, which are potent antioxidants might protect the brain against oxidative stress produced by hypobaric hypoxia. The brain effects of three doses of grape seed extract intraperitoneally (i.p.)administered in rats after exposure to hypobaric hypoxia corresponding to 5500m altitude were investigated. Some oxygen and nitrogen reactive species, inflammatory cytokine (IL-6) and molecules involved in angiogenesis (vascular endothelial growth factor [VEGF], matrix metalloproteinase 2 [MMP2], and tissue inhibitors of metalloproteinase 1 [TIMP1]) were determined. Forty-two rats were divided in seven groups: group 1, control; groups 2, 3, and 4 were exposed to hypobaric hypoxia for 24 h in a hypobaric chamber; groups 5, 6, and 7 were exposed to hypobaric hypoxia for 5 days. After returning to normal atmospheric pressure, rats from groups 2 and 5 were sacrificed without other treatment. Animals from groups 3and 6 were i.p treated with carboxymethyl cellulose (CMC) vehicle and those from groups 4 and 7 were i.p. treated with grape seed extract (GSE) (50 mg gallic acid equivalents/kg body weight in 0.5mL CMC suspension/animal). The treatment was applied at 2, 24, and 72 h from returning to normoxia. Hypobaric hypoxia produced increased brain levels of ROS,nitric oxide (NO), IL-6, and VEGF after both time intervals (P < .05). The MMP2 concentration was significantly increased in groups treated only with vehicle, whereas TIMP1 was slightly changed. GSE produced a significant reduction of ROS and NO levels proving its antioxidant capacity. It also decreased IL-6 and MMP2 concentrations to values similar to controls. The VEGF concentration was also significantly reduced. These effects are indicative for anti-inflammatory and antiangiogenic properties of GSE.
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Biological Effects of Hydrolyzed Quinoa Extract from Seeds of Chenopodium quinoa Willd.
Quele Adriana Meneguetti,Mislaine Adriana Brenzan,Marcia Regina Batista,Roberto Barbosa Bazotte,Daniel Rodrigues Silva,Diógenes Aparício Garcia Cortez 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.6
An extract from seeds of Chenopodium quinoa Willd. (quinoa), termed hydrolyzed quinoa (HQ), was obtained by enzymatic hydrolysis from seeds of the quinoa variety BRS-Piabiru. Analysis of the physical and chemical properties of quinoa and HQ showed that the hydrolyzed extract is rich in essential amino acids, particularly those with branched chains (leucine, isoleucine, and valine). In addition, we evaluated the biological effects of HQ, particularly the toxicological potential. For this purpose, male Wistar rats were assigned randomly to four groups: (1) sedentary supplemented group, which received HQ (2,000 mg/kg); (2) sedentary control group, non-supplemented; (3) exercised supplemented group (i.e., rats subjected to aerobic physical exercise that received HQ [2,000 mg/kg]); and (4) exercised control group (i.e., rats subjected to aerobic physical exercise, non-supplemented). After 30 days, all groups were analyzed for levels of serum glucose, cholesterol, triacylglycerol, total protein, albumin, uric acid, and urea and activities of the enzymes alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase. Body weight gain, dietary intake, and lipid deposition were also analyzed. The results showed no hepatic and renal toxicity of HQ. Moreover, decreased food intake, body weight, fat deposition, and blood triacylglycerol level were observed in the supplemented groups (sedentary and exercised supplemented groups). These results suggest a potential use of HQ in human nutrition.
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An Analysis of the Mediterranean Cruise Ports’ Network Using Social Network Analysis
Adriana Estefania Valero Polasek,Tae-Hyeon Yang,Sung-Hoon Park,Gi-Tae Yeo 한국항해항만학회 2020 한국항해항만학회지 Vol.44 No.2
The cruise industry in the Mediterranean region increased from 2000-2018, being the second most important region after the Caribbean. The purpose of this study was to analyze the networks and hub ports of the Mediterranean. This paper used the SNA (Social Network Analysis) methodology, which includes Hub and Authority Combined Centrality (HACC) that has been used to analyze cruise port centrality, as well as degree centrality such as In-Degree, Out-Degree, and Betweenness. This empirical study suggests that the top three ports of the Mediterranean ports’ network in terms of hub index are Barcelona, Civitavecchia, and Palma de Mallorca. The academic implications are the suggestion for data integration based on real itineraries and numbers of POC (Port of Calls), as well as the selection of the hubs of the targeted areas. The practical implications are suggested such as a clear requirement for cruise industry, as a way to widen the scope for the Mediterranean region and a valuable reference for cruise ship companies to select the best fit home ports.
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De creonte(s) antígona(s) y caliban(es):ética y eticidad en José Martí
Adriana C. Rodríguez 한국외국어대학교 국제지역연구센터 2016 국제지역연구 Vol.20 No.2
The paper approaches the thought of José Martí in order to identify and analyze some of their ethical traits as bearers of a new ethics. The search for this new morality stands as an essential and dynamic topic that crosses all his ideas and is expressed in several of his productions. As emblematic examples: El Presidio Político, Madre América, Nuestra América y El Manifiesto de Monte Cristi. However its most important function is to establish a new awareness about the resistance and struggle in a context framed in the independence of Cuba and the Antilles.
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Adriana Basile,Barbara Conte,Daniela Rigano,Felice Senatore,Sergio Sorbo 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.1
Feijoa sellowiana Berg. fruit, widely used for human consumption, is well appreciated for its good nutritional characteristics and for its pleasant flavor and aroma. In a prior study we showed that the acetonic extract of F. sellowiana fruit exerts a potent antibacterial activity against some Gram-positive and Gram-negative bacterial strains. To confirm and deepen the study on antimicrobial activities of the fruit, in this article we analyze the same extract for its effectiveness against different kind of bacteria both as American Type Culture Collection standard strains and clinically isolates, the Gram-negative Helicobacter pylori, and three fungal strains (one human pathogen and two phytopathogens). Our aim was also to isolate and define the active component(s) involved in the antibacterial, antifungal, and anti-H. pylori activity of the extract through activity-guided fractionation procedures. This allowed us to identify flavone as the active compound of F. sellowiana fruit. Flavone showed a high antibacterial activity against the nine standard bacterial strains tested and the matched clinically isolated bacterial strains and was significantly more active against H. pylori than metronidazole. Pseudomonas aeruginosa, Proteus mirabilis, and Proteus vulgaris from both standard and clinical sources showed a very high sensitivity to flavone (minimal inhibitory concentration [MIC]=1.95μg/mL for standard strains and 3.9μg/mL for clinical isolates of the three bacteria), showing also good minimal bactericidal concentration values. Among fungi tested, Rhyzoctonia solani was the most sensitive strain to the action of flavone (MIC=62.5μg/mL).
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Effects of dietary fibers and prebiotics in adiposity regulation via modulation of gut microbiota
Adriana Rivera‑Piza,이성준 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.1
The microbiota is indispensable for human health and the regulation of various body functions, including energy metabolism. The harmonic crosstalk between the microbiota and the intestinal epithelial barrier determines gut homeostasis and health status in the healthy subject. Obesity and type 2 diabetes risk are, to some extent, explained by alterations in the microbiota. Since recent data indicate that the population of gut microorganisms can influence nutrient absorption and energy storage thus prevalence on obesity and metabolic disorders. Moreover, metabolic disease conditions, such as obesity, may be stimulated by genetic, environmental factors and by pathways that link metabolism with the immune system. On the basis of the above considerations, this review compiles the current results obtained in recent studies indicating the gut microbiota contribution to obesity development.
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