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서울동물원 야생동물의 임상 검체 내 Clostridium 균의 항생제 내성 분석
이하늬 ( Hany Lee ),여용구 ( Yong-gu Yeo ),안상진 ( Sangjin Ahn ),김종택 ( Jong-taek Kim ) 한국동물위생학회(구 한국가축위생학회) 2020 韓國家畜衛生學會誌 Vol.43 No.1
Clostridial bacteria are zoonotic agents, which cause severe necrotizing enteritis, pseudo-membrane colitis, enterotoxemia to both humans and animals. The objective of this study was to monitor the antibiotic resistance of Clostridium isolates on clinical specimens from wild animals in Seoul zoo for 5 years. Clostridium isolates were verified by using Vitek2 compact machine. Antibiotic susceptibility was assessed by antibiotic disc diffusion test, which was followed by Kirby-Bauer disc diffusion test method. The frequency of Antimicrobial resistance of Clostridium isolate was the greatest in gentamicin (87%), then in order of amikacin (80%). There were 55.6% of Clostridium isolates showed multiple drug resistance (MDR). These results showed that a lot of Clostridial bacteria from wild animals in Seoul zoo were acquired antibiotic resistance. Because of the wild animal’s aggressive manner, it has been hard to collect clinical samples from wild animals in a zoo to exam antibiotic susceptibility. For these reasons, empirical use of antibiotics has been performed in frequently. It may cause to increase the emergence of antibiotic resistance bacteria. In addition, the antibiotic resistance bacteria from zoo animals can be spread to other wild animals which inhabit around the zoo. Therefore, regular monitoring of antibiotic resistance Clostridial bacteria is important to protect animals and humans from Clostridial diseases.
PCR을 이용한 salmonella enteritidis의 특이적 검출
이정학 ( Jung Hak Lee ),이병동 ( Byung Dong Lee ),조미영 ( Mi Yeong Jo ),여용구 ( Yong Gu Yeo ),김영섭 ( Young Seb Kim ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.3
Salmonella enteritidis is the most prevalent etiologic agents of foodhome acute gastroenteritis. Direct isolation and identification of S enteritidis are time consuming work and not so highly sensitive. This study was conducted to develop for the specific detection of S enteritidis using polymerase chain reaction(PCR). PCR primers were selected to amplify a 351 -base pair(bp) DNA fragment from the salmonella plasmid virulence A(spv A) gene of S enteritidis. With the primers, 351 bp DNA products were amplified from S enteritidis but not from other B, D, Cl serogroup Saimoneilci spp. It was sensitive to detect up to 40 pg of template DNA by agarose gel electrophoresis. This PCR assay is very rapid and specific method and less time consuming than the standard bacteriological methods.