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쌀단백질(蛋白質)에 관(關)한 연구(硏究) (제2보(第2報)) 갱나수륙도(粳?水陸稻)의 미단백질(米蛋白質) 획분정량(劃分定量)
이춘영,김수일,김성곤,Lee, C.Y.,Kim, S.I.,Kim, S.G. 한국응용생명화학회 1969 Applied Biological Chemistry (Appl Biol Chem) Vol.12 No.1
The fractions of protein, albumin, globulin, oryzenin, and prolamine extracted from 20 varieties of rice were determined, which include upland and paddy rice with both waxy and nonwaxy types. The protein content is higher in the waxy type and especially in upland rice. The content of oryzenin and albumin were likewise higher in the waxy and upland types. The results were discussed in the view point of nutrition. 한국산(韓國産) 벼 20품종(品種)(주(主)로 도입(導入) 품종(品種)) 즉 수륙도(水陸稻) 찹쌀 및 멥쌀 각(各) 5품종(品種)씩에 대하여 그 조단백질량(粗蛋白質量)과 각획분(各劃分) 단백질의 량(量)을 분석 검토하였다. 멥쌀보다 찹쌀 그리고 수도(水稻)보다 육도(陸稻)가 평균으로 단백질함량이 많았으며 oryzenin 과 albumin의 각(各) 함량비(含量比)도 같은 경향을 보였다.
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Variability of Protein Content in Rice Grown at Several Different Environments
허문회,이춘영,최진용,김수일,Heu, M.H.,Lee, C.Y.,Choe, Z.R.,Kim, S.I. The Korean Society of Crop Science 1969 한국작물학회지 Vol.- No.7
Protein content of Korean native and currently recommended varieties varied from 5.3% to 9.4%. Higher protein content was shown in Indicas than Japonicas and generally in the earlier varieties than in the latter ones. Additional application of nitrogen significantly increased the protein content, and varietal difference in this response was noticed. Delayed harvesting reduced protein content and denser planting induced higher protein content in the brown rice.
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金秀一,李錫河,李弘石,文亢植,羅志英 서울大學校 農科大學 1985 서울대농학연구지 Vol.10 No.1
대두의 단백추출액을 polyacrylamide gel 전기영동에 의하여 분류하고 trypsin inhibitor (T.I) band를 동정하였다. T.I. band는 전기영동한 gel을 trypsin으로 가수분해하거나 추출액에 trypsin을 처리한 후 전기영동하거나 발색기질을 이용하여 gel을 착색시키거나 또는 gel slice의 T.I.activity를 측정하는 등 네 가지 방법을 사용하여 검정하였다. 이중 추출액을 trypsin으로 처리한 후 전기영동하는 방법과 gel slice의 T.I.activity를 측정하는 방법이 가장 적합하였으며 두 방법의 결과를 비교하여 T.I.band를 검정하는 것이 보다 확실하였다. Sephadex G-75 Chromatography 에서 물로 추출한 대두 단백질은 3 fraction으로 분리하였고 T.I.activity는 제 2 fraction 에만 나타났다. Kunitz 및 Bowman-Birk형 inhibitor는 DEAE-cellulose column chromatography로 분리하였다. Kunitz형은 5개의 fraction으로, Bowman-birk형은 4개의 fraction으로 분리되었다. 단백질 추출액과 DEAE-cellulose chormatography에서 분리된 Kuniz 및 Bowman-Birk T.I.의 polyacryamide gel 전기영동 pattern을 비교하여 본 결과, 확실하게 동정된 T.I.band는 band3과 band4로서 각각 Orf등이 발표한 Ti¹과 Ti2에 해당하였으며, 그 외에 band 6과 band 10이 T.I.로 추정되었고 band 1,2,5,7,8,9는 T.I.가 아닌 것으로 판명되었다. trypsin inhibitor 함유량은 총 trypsin units inhibited 값(T.U.I)으로 볼 때 42품종에서 25에서 76까지 품종 간에 차이가 현저하였으며 시비 및 파종기의 영향은 나타나지 않았다. Ti¹ inhibitor 를 보유하고 있는 것은 37품종이었고, Ti²를 보유하는 것은 7품종이었으며, Ti¹과 Ti²를 같이 가지고 있는 품종은 발견되지 않았다. 이러한 품종의 Ti¹,Ti² 보유 pattern은 재배조건에 의해 변화되지 않았다. 2조합의 pattern은 재배조건에 의해 변화되지 않았다. 2조합의 정역교배에서 얻은 F₁ 종자의 전기영동 pattern을 비교해 본 결과, Ti¹품종끼리의 교배종자에서는 정역교배에 상관없이 Ti¹ inhibitor 만 나타났고 Ti¹품종과 Ti²품종의 교배종자에서는 Ti²를 모본으로 한 종자에서는 Ti¹과 Ti² 두 inhibitor가 검출되었으나 여교배에서는 모본의 Ti¹ inhibitor만 검출되었다. 여교배에서 Ti¹만 나타난 것은 분석시료 종자가 적었고 교배의 여부를 확인할 수 없어 모본의 세포질적 영향에 의한 것인지 또는 자가수정에 의한 것인지 분명치 않았다. The protein extracts from soybean seeds were examined by polyacrylamide gel electrophoresis and the trypsin inhibitor (T.I) bands were detected. The water-extractable protein was fractionated into three fractions by Sephadex G-75 gel filtration. The T.I activity was found only in the second fraction. Kunitz and Bowman-Birk inhibitors were fractionated by DEAE-cellulose chromatography into seven and six fractions, respectively. In kunitz inhibitor, 5 fractions were found to have T.I activity and 4 fractions in Bowman-Birk inhibitor. From the and patterns of the protein extracts and those of DEAE-cellulose chromatographic fractions, it was found that band 3 and 4 were T.I. band, corresponding to Ti¹ and Ti² band, respectively. In addition, band 6 and 10 were presumed to be T.I. band. Of the 42 varieties sampled, 35 revealed only Ti¹ band and 7 only Ti² band. The T.I. band patterns were not changed by the culture condition. The T.I. content, when expressed as the number of trypsin units inhibited (T.U.I), showed remarkable differences from 25 to 76 between varieties. The seedtime and fertilization condition had no effect on the T.I. content. Judged from the results of F ₁seeds analysis, we assumed that Ti¹ and Ti²band were controlled by codominant allele at a single locus.
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돼지난소에서 난포폐쇄시 과립층세포의 아포토시스와 대식세포에 관한 형태학적 연구
박창식,한승로,김수일,조근자,김원식 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8
It is known widely that granulosa cell apoptosis leads follicu1ar atresia and macrophages exert their effects directly and/or indirectly from the initiation to the completion of follicular atresia by phagocytosis of apoptotic bodies and secretion of various cytokines. However, the site of initiation, propagation routes and the elimination methods of apoptotic bodies, and the time and methods of penetration of macrophages into the follicles are not known completely. Using pig(Yorkshire-breed) ovary, immunohistochemical studies with TUNEL far apoptotic bodies and pig macrophage monoclonal antibody 4E9 for macrophages, and light and transmission election microscopic observations were performed. In the pig, follicular atresia began with the granulosa cell apoptosis, and the apoptosis of theca intema cells occured at the same time. The apoptosis of granulosa cells initiated randomly within the granulosa cell layer and propagated rapidly into the whole layer. Ultrastructurally, apoptotic ranulosa cells showed characteristic pyknotic and deformed nucleus and intracytoplasmic vesicles. Apoptotic bodies were eliminated by intact granulosa cells and macrophages. Intact granulosa cells inserted apoptotic bodies transiently, soon after they fell into the apoptotic. Finally, apoptotic bodies and degenerated oocyte were phagocytosed by macrophages. Macmphages entered the ovarian follicle at the time of initiation of granulosa cell apoptosis, and migrated with the progession of apoptosis. By elimination of theca cells, macrophages contributed the completion of follicular atresia. These results will provide valuable informations on the study of the interrelation between mac개phage and ovarian follicular atresia.
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돼지난소에서 난포폐쇄시 과립층세포의 아포토시스와 대식세포에 관한 형태학적 연구
박창식,한승로,김수일,조근자,김원식 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10
It is known widely that granulosa cell apoptosis leads follicular atresia and macrophages exert their effects directly and/or indirectly from the initiation to the completion of follicular atresia by phagocytosis of apoptotic bodies and secretion of various cytokines. However, the site of initiation, propagation routes and the elimination methods of apoptotic bodies, and the time and methods of penetration of macrophages into the follicles are not known completely. Using pig(Yorkshire-breed) ovary, immunohistochemical studies with TUNEL for apoptotic bodies and pig macrophage monoclonal antibody 4E9 for macrophages, and light and transmission electron microscopic observations were performed. In the pig, follicular atresia began with the granulosa cell apoptosis, and the apoptosis of theca intema cells occured at the same time. The apoptosis of granulosa cells initiated randomly within the granulosa cell layer and propagated rapidly into the whole layer. Ultrastructurally, apoptotic granulosa cells showed characteristic pyknotic and deformed nucleus and intracytoplasmic vesicles. Apoptotic bodies were eliminated by intact granulosa cells and macrophages. Intact granulosa cells ingested apoptotic bodies transiently, soon after they fell into the apoptosis. Finally, apoptotic bodies and degenerated oocyte were phagocytosed by macrophages. Macrophages entered the ovarian follicle at the time of initiation of granulosa cell apoptosis, and migrated with the progression of apoptosis. By elimination of theca cells, macrophages contributed the completion of follicular atresia, These results will provide valuable informations on the study of the interrelation between macrophage and ovarian follicular atresia.
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돼지 황체에서 TNFα-분비 대식세포와 내피세포에 관한 면역조직화학적 연구
박창식,한승로,김수일,조근자,김원식 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10
In the corpus luteum, TNFα is known to induce functional and structural luteolysis. In addition, it acts as luteotropic agent during the initial and early stage of luteal development. In spite of its importance in corpus luteal development, there is still different opinions for the source cells of TNFα in the corpus luteum. One is the macrophages only, and the other is macrophages are the main source and endothelial cells are the minor source. In this experiment, using the porcine corpora lutea of pregnancy and ovulatory stages, hematoxylin-eosin stain, macrophage and TNFαimmunohistochemistry were carried to reveal the sources of TNFα. As a result, MAC 387-positive macrophages were present in all the stages of corpora lutea. In the mature corpora lutea of nonpregnant stages, the sites of MAC 387-positive macrophages and those of TNFα-positive macrophages were coincided, and the sites of endothelial cells and those of TNFα-positive endothelial cells weie nearly coincided. But, in the mature CL of pregnant stage, mid- and advanced luteolytic stages of both nonpregnant and pregnant stages, the sites of MAC 387-positive macrophages and those of TNFα-positive macrophages were coincided, but not in the endothelial cells. Accordingly, it can be concluded that macrophages are the main source of TNFα in the corpus luteum and endothelial cells are the minor source in the mature and mid-lytic stages, but, in the advanced luteolytic stage, macrophages are the only source of TNFα.
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Trypsin inhibitor 결여大豆品種의 탐색 및 그의 遺傳育種學的 硏究 : Ⅱ. Kunitz 및 Bowman-Birk trypsin inhibitor의 정제 및 분리
金秀一,이홍석 서울大學校 農科大學 1986 서울대농학연구지 Vol.11 No.1
Crude Kunitz and Bowman-Birk soybean trypsin inhibitors were fractionated into three fractions by Sephadex G-75 gel filtration respestively. The Kunitz and Bowman-Birk trypsin inhibitors from Sephadex G-75 column chromatography were resolved into nine and eight fractions by DEAE-cellulose chromatography respectively. All the fractions except ninth fraction of Kunitz inhibitors revealed trypsin inhibiting actvities. Each fraction isolated was examined their polyacrylamide gel electrophoretic patterns with or without SDS. Among 10 main bands, five were appeared as trypsin inhibitors. Kunitz inhibitors differ from Bowman-Birk inhibitors in their electrophoretic patterns, showing band 3 and 4 as main bands instead of band 6,7,8 and 9 for Bowman-Birk trypsin inhibitors. The moleculer weight of Kunitz trypsin inhibitors was 22 Kdaltons while that of Bowman-Birk inhibitors was lower than 14 Kdaltons. In addition, Bowman-Birk type inhibits chymotrypsin twice as much as Kunitz type inhibitors.
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Trypsin inhibitor 缺如大豆品種의 探索 및 그의 遺傳育種學的 硏究 : Ⅲ. Trypsin inhibitor의 活性度 및 電氣泳動樣相의 品種間 差異와 그들의 遺傳現象
이홍석,金秀一 서울大學校 農科大學 1986 서울대농학연구지 Vol.11 No.1
To obtain the basic information on the trypsin inhibitors of soybean seeds, the activity and electrophoretic band patterns of trypsin inhibitors were investigated. Materials tested were the seeds of 81 varieties including 71 local collections, F₁seeds of diallel cross of 6 varieties and F₂seeds of 2 crosses. The results are summarized as follows: 1. Among 81 varieties and lines tested, 64.2%, 16.0%, and 16.0% of the varieties indicated Ti¹,Ti²and both Ti¹and Ti²band, respectively. Raiden and Nemasilazu had only Ti³band and Geumdu was the only variety lacking of Kunitz trypsin inhibitor band. 2. Varietal differences in the trypsin inhibitor activity were highly significant, ranging from minimum of 18182 to maximum of 71000. The trypsin inhibitor activity was also considerably variable among varieties which have same trypsin inhibitor band of Ti¹and Ti². But three varieties which were lack of any Kunitz trypsin inhibitor band and only Ti³band indicated lower trypsin inhibitor activity. 3. Trypsin inhibitor band of Ti¹and Ti²was genetically controlled by codominant allele system, indicating 1:2:1 segregation ratio of Ti¹:Ti²:Ti²bands in F₂seeds.
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