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Coal ash is produced at rate of 12.20% by weight of the original coal when coal is burned to produce steam for electricity generation. Korea consumes approximately 42 million tons of coal annually to generate electricity, and generates around 6 million tons of coal ash annually. However, only 60% of the coal ash by.products are recycled, while 40% are buried at the power plant site. In particular, most of bottom ash was landfilled, and then properly recycling methodology should be developed. Bottom ash is well known as soil amendment and high organic matter (ca. 0.3.15%) containing material. However, the function of organic matter containing in coal bottom ash as it applied in soil as an amendment was not studied well. In this study, in order to evaluate the properties of ash containing organic matter when it applied in soil as amendment, the chemical characteristics of humic substances in the coal bottom ash mixed soils were compared with the pure soil. We collected two types of soil from the landfill cover simulator which is bottom mixed soil (BA35% + soil 65%) and only soil (100%) treatment in the 5th year after installation. The soil organic matter was fractionized with humic, fulvic acid and humin by using the sequential extraction method. The chemical structure of humic substances was characterized by 13C NMR spectroscopy. Bottom ash mixed soil has 5 times higher total organic C content than the pure soil. In addition, humic substance C content was also higher in the bottom ash mixed soil with the similar composition ratio with total organic C content. However, there was no significant difference of chemical composition of humic substances between the bottom ash mixed soil and the pure soil. Aromatic group of humic acid and fulvic acid was most dominant with around 30% of TOC, and then followed by O.alkyl (126.96.36.199%), Alkyl (188.8.131.52%), and carboxyl OC groups (184.108.40.206%). Conclusively, the mineralogical effect of BA might be a main factor on accumulating SOM in BA mixed soil layer.