이환자돈으로부터 오제스키병 바이러스 분리와 생물학적 성상

전무형,조성환,안수환,박성국,윤석민,하용공,Jun Moo-Hyung,Cho Sung-Whan,An Soo-Hwan,Park Seong-Kuk,Yoon Seog-Min,Ha Yong-Kong 대한수의사회 1988 대한수의사회지 Vol.24 No.3

A swine farm located in Namyangju, Kyunggi-do was damaged by increased loss due to stillbirth, abortion and high mortality of suckling pig during October to December 1987. Three piglets of the farm, that were diagnosed clinico-pathologically to be affecte

Dot-immunoassay를 이용한 Aujeszky's disease virus항원 및 항체 검출

전무형,조용성,장경수,Jun, Moo-hyung,Cho, Young-sung,Chang, Kyung-soo 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.3

To establish more specific and simple diagnostic methods for detection of the antibodies and antigens of Aujeszky's disease virus(ADV), we designed indirect dot-immunoassay(IDI) and double sandwich dotimmunoassay(DSDI) using the solid phases of nitrocellolose paper and polystyrene plate. The diagnostic efficacy of these methods was investigated. As the sensitivity of IDI was tested by various virus concentration, the specimens with the virus titer above $10^{4.0}TCID_{50}/0.2ml$ showed positive reaction, but that below $10^{1.0}TCID_{50}/ml$ revealed negative. Tonsil emulsion at the virus titer of $10^{4.5}TCID_{50}/0.2ml$ showed the highest sensitivity as diluted by 1/100. In detection of ADV antigens from the various tissues of the rats and pigs infected with ADV, IDI using monoclonal antibody showed the higher specificity as compared with IDI using polyclonal antibody and virus isolation method. The efficacy of the DSDI for detection of ADV antibody was compared with other tests. The sensitivity of DSDI was higher than virus neutralization(VN) and agar gel immunodiffusion test(AGID). Meanwhile, specificity of DSDI was lower than AGID, but similar to IDEA. In comparison with VN test, DSDI showed 96.9% agreement to VN test that is the highest of three tests. In general, application of polyclonal antibody in both tests caused the higher sensitivity but the lower specificty.

단(單)클론성 항체(抗體)를 이용한 소전염성비기관염(傳染性鼻氣管炎)바이러스 진단법(診斷法) 개발 I. 소전염성비기관염(傳染性鼻氣管炎)바이러스에 대한 단(單)클론성 항체(抗體) 생산(生産)

전무형,김덕환,이헌준,안수환,권창희,Jun, Moo Hyung,Kim, Duck Hwan,Lee, Hun Jun,An, Soo Hwan,Kweon, Chang Hee 충남대학교 농업과학연구소 1987 Korean Journal of Agricultural Science Vol.14 No.2

세포융합기술(細胞融合技術)을 이용하여 소전염성비기관염(傳染性鼻氣管炎)바이러스(IBRV) 항원(抗原)에 대응(對應)하는 9주(株) 단(單)클론성항체(性抗體)를 생산하였으며, 이들 항체(抗體)의 특성(特性)을 형광항체법(螢光抗體法), 혈청중화시험(血淸中和試驗) 및 전기영동분석법(電氣泳動分析法)으로 시험(試驗)하였다. 생산된 2종(種)의 단(單)클론성 항체(抗體)중 8종(種)은 IBRV이외의 Pseudorabies virus, Infectious laryngotracheitis virus, Marek's disease virus, Japanese B encephalitis virus, Porcine parvovirus, Transmissible gastroenteritis virus, Hogcholera virus와 교차반응(交叉反應)이 없는 특이항체(特異抗體)였으나 1종(種)은 Pseudorabies virus와 교차(交叉)하는 항체(抗體)였다. 이들 단(單)클론성 항체중 중화능력(中和能力)이 있는 2주(株)는 IBRV 항원단백(抗原蛋白)중 분자량 72K 또는 125K 달톤의 항원(抗原)에 대응하는 항체(抗體)였으며, 94K 달톤의 항원(抗原)에 대응하는 단(單)클론성 항체(抗體)는 중화력(中和力)이 인정되지 않았다. 한편 1종의 교차반응성(交叉反應性) 항체(抗體)는 분자량 100K 달톤의 IBRV 항원(抗原)과 40K의 Pseudorabies virus 항원(抗原)에 대응하는 항체(抗體)로 밝혀졌다. 생산된 항체(抗體)중 중화력(中和力)이 있고 IBRV 항원(抗原)의 72K 달톤 단백질(蛋白質)에 대응하는 7-C-2 항체(抗體)를 이용하여 야외분리주(野外分離株) IBRV의 항원(抗原) 동정(同定)이 가능(可能)하였다. Nine monoclonal antibodies directed against infectious bovine rhinotracheitis virus (IBRV) were prepared by using cell hybridization technique, and the biological properties of the antibodies were investigated by means of immunofluorescence, serum neutralization, and electrophoretic analysis. Eight of 9 monoclonal antibodies reacted specifically with the antigenic constituents of IBRV, infectious laryngotracheitis virus, Marek's disease virus, turkey herpesvirus, hog cholera virus, porcine parvovirus and transmissible gastroenteritis virus. However, the remaining one, 26-2 clone, was found to be cross-reactive with pseudorabies virus. Two monoclonal antibodies, 7-C-2 and 12-A-2, which had neutralizing activity, were reactive with the molecular weights of 72 kilo daltons (72K) and 125K of IBRV proteins electrophoretically separated, respectively. The monoclonal antibody, 3-H-3, which is corresponding to 94K of IBRV proteins, revealed no neutralizing activity. The cross-reactive monoclonal antibody, 26-2, was proved by electrophoretical analysis to be reactive with 100K of IBRV proteins and 40K of pseudorabies virus.

소 전염성비기관염(傳染性鼻氣管炎) 바이러스에 대한 monoclonal antibody 생산(生産)과 진단법(診斷法) 개발 II. Monoclonal antibody를 이용한 소 전염성비기관염(傳染性鼻氣管炎)의 진단(診斷)

전무형,김덕환,안수환,이중복,민원기,Jun, Moo-hyung,Kim, Duck-hwan,An, Soo-hwan,Lee, Jung-bok,Min, Won-gi 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.1

To develop more specific and sensitive diagnostic methods for infectious bovine rhinotracheitis, 7-C-2 monoclonal antibody specific to polypeptides of infectious bovine rhinotracheitis virus (IBRV) was applied in indirect immunofluorescence antibody assay (IFA), indirect immunoperoxidase assay(IPA) and radial immunodiffusion enzyme assay (RIDEA). It was found that IBRV infected in MDBK cells could be detected as early as 8 hours post infection by IFA, and that IFA was more rapid and specific to identify IBRV antigen than IPA. The diagnostic efficacy of RIDEA and SN test was studied with 88 bovine sera. It was evident that RIDEA could eliminate the false positive reaction encountered in serum neutralization(SN) test, being more rapid and sensitive than the latter. Highly significant correlation coefficiency (r=0.76, p<0.01) was evaluated between the titers of sera and the diameters of RIDEA. Tracheal membranes and sera collected from 96 slaughtered cattle with lesions in respiratory organs were examined to detect IBRV antigen and antibody by IFA, RIDEA and SN test. It was presented that positive rates were 32.3% in IFA, 20.8% in RIDEA and 21.9% in SN test, and that coincidence rate between RIDEA and SN test were 100% in positive sera and 98.7% in negative sera. In conclusion, it was assumed that application of monoclonal antibody could improve the diagnostic efficacy of IBR by enhancing sensitivity and specificity of IPA, IFA and RIDEA.

Ovine Squamous Cell Carcinoma: A Review

전무형,로버트 죤슨,Jun Moo Hyung,Johnson Robert H. Korean Veterinary Medical Association 1980 대한수의사회지 Vol.16 No.2

양린상세포암은 태양자외선에 기인하여 발생되며 털이 엷게 덮혀 있는 체표면에 다발하는 악성피부암이다. 이 종설은 이 종양의 발병기인, 병리학적과정, 지리학적분포, 숙주와 종양의 면역관계 및 처치와 관리에 관연된 문헌을 수록하였다. Ovine squamous cell carcinoma(OSCC) is a naturally occurring malignant tumour, being considered due to solar radiation. The literature on OSCC is reviewed, pertaining to the following five aspects of the disease; aetiology, tumour development, geographica

SefA 유전자 PCR에 의한 Salmonella serogroup D1의 특이적 검출

전무형,김태중,장경수,강경임,김귀현,김기석,유상식,김현수,신광순,김철중,Jun, Moo-hyung,Kim, Tae-joong,Chang, Kyung-soo,Kang, Kyong-im,Kim, Kui-hyun,Kim, Ki-seok,Yoo, Sang-sik,Kim, Hyun-soo,Shin, Kwang-soon,Kim, Chul-joong 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.3

Sal enteritidis thin fimbriae, SEF14, were found to be restricted to the predominantly poultry-associated members of the Salmonella serogroup D1 that are considered as the important pathogens in poultry industry. SefA together with sefB and sefC encode the proteins involved in SEF14 biosynthesis. In order to develop the rapid and specific detection methods for Salmonella serogroup D1, a PCR technique for the amplification of sefA gene was established, and its specificity and sensitivity were investigated with various microorganisms. The bacterial genomic DNA was extracted by colony-picking and rapid boiled-lysate technique. In comparison of Sef I and Sef II primers used in the PCR, Sef I primer for sefA gene of 513bp showed higher specificity than that of Sef II. The established PCR was as sensitive as to detect 1pg of Sal enteritidis DNA. When 73 strains in 28 genera including the reference strains and the field isolates of various Salmonella serotypes, Bacillus subtilis, Bordetella bronchisepdca, E coli, Listeria spp., Micrococcus luteus, Rhodococcus equi, Staphylococcus spp., Streptococcus spp., Vibrio parahemolyticus, Yersinia spp. were studied, the established PCR yielded specifically positive results with only Salmonella serogroup D1. The results suggested that the PCR for sefA gene could be a potential candidate among the specific detection methods for Salmonella serogroup D1.

Seroepizootiological Study on Bovine Leucosis in Korea

전무형,정운익,이창구,백순용,임창형,Jun, Moo-Hyung,Chung, Un-Ik,Lee, Chang-Ku,Baig, Soon-Yong,Lim, Chang-Hyeong The Korean Society of Veterinary Science 1982 大韓獸醫學會誌 Vol.22 No.2

소백혈병(白血病)바이러스 봉입체단백(封入體蛋白)을 항원(抗原)으로 이용한 면역확산법(免疫擴散法)으로 전국에 산재해 있는 164개 목장(牧場)에서 사육중인 2003두(頭)의 유우(乳牛)에 대한 소백혈병(白血病)바이러스항체(抗體) 조사결과, 양성율(陽性率)은 충청도(忠淸道)가 41.8%로 가장 높았고, 전북(全北)은 24.4%로 가장 낮았다. 지역별(地域別) 양성율(陽性率)은 중부지역(中部地域)이 37.8%, 호남지역(湖南地域)이 27.2%, 영남지역(嶺南地域)이 28.0%, 그러고 영동지역(嶺東地域)이 25.2%였다. 전국의 평균 양성율(陽性率)은 29.8%였다. 혈청학적(血淸學的) 검사결과(檢査結果)를 분석(分析)하였던 바 양성율(陽性率)은 소의 연령(年齡)이 높을수록, 사육규모(飼育規模)가 클수록 높은 경향이 있었고, 소의 연령군(年齡群)이 6내지 8세에서, 사육규모(飼育規模)가 20내지 50두(頭)의 우군(牛群)에서 양성율(陽性率)이 가장 높였다. 중부지역(中部地域)에 사육중인 117두(頭)의 종모우(種牡牛)에 대해 조사한 결과 한우(韓牛)에서는 5.7%(4/70), 홀스타인 종모우(種牡牛)에서는 35.9% (14/39)의 양성율(陽性率)을 나타냈다. 우군별(牛群別)로는 혈청검사(血淸檢査)를 한 164개 우군(牛群)중에 양성우(陽性牛)가 전혀 없는 우군(牛群)이 17개군(個群)(10.4%), 20~40%의 양성우(陽性牛)가 있는 우군(牛群)이 42개(個)(25.6%)였고, 80%이상의 양성우(陽性牛)가 있는 우군(牛群)은 10개군(個群)(6.1%)이었다. 소백혈병(白血病)바이러스항체(抗體) 양성우(陽性牛)에서 분리(分離)한 임파구(淋巴球)를 phytohemagglutinin을 첨가한 배지(培地)에 단기배양(短期培養)한 후 BLV 형광항체(螢光抗體)를 이용하여 임파구(淋巴球)내의 BLV항원(抗原) 증명(證明)을 시도한 바, 양성우(陽性牛) 11두(頭)중 8두((頭)(72.7%)에서 특이(特異)한 BLV항원(抗原)이 규명(糾明)되었다. BLV 항체양성우(抗體陽性牛) 7두(頭)와 음성우(陰性牛) 4두(頭)에서 분리한 임파구(淋巴球)의 우태아비장세포(牛胎兒脾臟細胞)에 대한 syncytium 형성능(形成能)을 시험한 바, 양성우(陽性牛) 7두(頭) 중 5두(頭)(71.4%)의 임파구(淋巴球)가 syncytium을 형성(形成)하였다. 배양(培養)된 임파구(淋巴球)를 전자현미경(電子顯微鏡)으로 검사한 결과 6두(頭)의 양성우(陽性牛) 중 2두(頭)에서 90~110nm. 크기의 전형적(典型的)인 C형(型) 소백혈병(白血病)바이러스가 증명(證明)되었다. Since bovine lymphosarcoma causes considerable economic loss to the dairy industry, seroepidemiological survey on bovine leucosis virus (BLV) was carried out for the dairy herds throughout the country to observe the epidemiological situation of the disease by using immunodiffusion test. Attempts were simultaneously made to detect bovine leucosis virus in the lymphocytes from BLV antibody-positive cattle by means of fluorescent antibody techniques, syncytium assay and electron microscopy. In immunodiffusion test for BLV antibody in 2003 heads of dairy cattle selected randomly from 164 herds, the prevalence of positive reactors by regions were 37.8% in Central, 27.2% in Honam (Southwest), 28.0% in Youngnam (Southeast) and 25.2% in Youngdong (East coast)and averaging 29.7%. By provinces, Chungcheong appeared the highest prevalence of BLV antibody carriers (41.8%), while Jeonbug revealed the lowest incidence rate (24.4%). When the results of serological studies were analyzed by age groups and the sizes of herds, the number of reactors increased gradually with the advance in the age of cattle and the herd size. The highest rate of BLV carriers was found in the ages between 6 and 8 years, and in the size of herds with 20 to 50 heads. One hundred and seventeen breeding bulls from the central regions were tested for BLV antibody. Four out of 70 bulls (5.7%) of Korean cattle and 14 out of 39 bulls (35.9%) of Holstein were reactive for BLV antigens. Of 164 dairy herds examined, 17 herds (10.4%) have no BLV antibody-positive cattle, while 42 herds (25.6%) were included in the range of 20 to 40% of the positive rate and 10 herds (6.1%) in the range of over 80% of the rate. When the lymphocytes from the BLV antibody carrying cattle were cultured in the presence of phytohemagglutinin and stained with FITC-conjugated sheep anti-BLV serum, 8 out of 11 cases (72.7%) of BLV positive cattle revealed specific fluorescence for BLV in the lymphocytes. In syncytium assay of the peripheral lymphocytes of the cattle, 5 out of 7 (71.4%) lymphocytes from BLV antibody carriers induced syncytia in the indicators of bovine embryonic splenic cells. The cultured lymphocytes were examined with an electron microscope to detect the BLV particles. Two out of 6 specimens (33.3%) from the reactors showed the typical type C virus with the size of 90 to 110 nm around microvilli and in intracytoplasmic vacuoles.

Current Status of Rabies Control in Korea

Moo Hyung Jun(全茂炯),Yong Hee Kim(金龍熙) 한국예방수의학회 1983 예방수의학회지 Vol.7 No.2

가축 특히 개의 狂犬病 발생은 1977년이후 현저히 감소되어 1982년에는 1건만 발생되었다. 발생의 地域的分布는 주로 중부 경기지역에 局限되고 있어, 그동안의 정부와 민간차원의 狂犬病 撲滅事業이 많은 효과를 거두고 있는 것으로 나타났다. 과거 5년간 중부 휴전선일대에만 광견병이 계속 발생되고 있는 사실로 보아 非武裝地帶의 野生動物이 본 병 傳播에 주요한 역할을 하고 있는 것으로 사료된다.

Tumour Specific and Immunosuppressive Components in Soluble Cell Extracts from Ovine Squamous Cell Carcinoma

Moo Hyung Jun(全茂炯) 충남대학교 농업과학연구소 1986 農業技術硏究報告 Vol.13 No.2

Bovine ocular squamous cell carcinoma : A review

Moo Hyung Jun(全茂炯) 한국예방수의학회 1981 예방수의학회지 Vol.5 No.1

소의 眼部位에 發生하는 鱗狀細胞癌은 소의 腫瘍性疾病중에 가장 多發하는 것으로써 肉牛 특히 헤어포드種에서 그 發生率이 높다. 肉牛産業이 發達한 先進諸國에서는 本 病으로 基因된 經濟的 損失이 무척 큰 것으로 알려져 있다. 本 癌腫은 體外部에 露出돼 있고, 또한 사람의 固形癌腫과 性狀이 類似하여 사람 腫瘍性疾病硏究를 위한 한 實驗的 모델로써 看做되어서 그 病因學的, 疫學的 硏究가 많은 學者들에 의해 광범위하게 遂行됐었다. 특히 本 癌腫이 免疫療法에 의해 成功的으로 治療될 수 있다는 사실이 많은 學者들에 의해 報告된 사실은 사람이나 哺乳動物에 있어서 宿主와 癌의 關係를 免疫學的으로 糾明하는데 적절한 資料로 提供되고 있다. 本 綜說에서는 소鱗狀細胞癌에 대한 分類, 病因學的 硏究, 腫瘍發生過程, 地理學的 分布, 宿主와 腫瘍의 免疫關係, 治療 및 豫防에 關聯되어 그동안 報告된 文獻을 整理하고 分析하여 記述하였다.