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최희인,이경갑,윤영민,박성준,장정호,Choi Hee-in,Lee Kyung-kap,Yun Young-min,Park Seong-jun,Chang Jeong-ho 한국임상수의학회 1994 한국임상수의학회지 Vol.11 No.2
A consecutive Jiao Chio acupuncture therapy was performed for 3 days in the 45-90 days old 11 calves of which have been shown severe watery diarrhea. The discharge of the infected calves was yellowish brown in color. Two calves of these patients were infected wi pulmonary disease as well as diarrhea. Thus, Su Qi and Fei Yu acupuncture therapy was carried out additionally after dosing with antibiotics twice for The two infected calves. Blood chemical values and serum neutralizing antibody titers were checked, and total blood cell count was also carried out to know the therapeutic effect before and after(21 days) acupuncture therapeutics. The results are as follows ; 1, The diarrhea has ceased one day after begining of the acupuncture therapy in 5 calves, and the cessation of the diarrhea in remaining calves occurred in 1 calf each on 3rd and 4th day, and 2 calves on 6th day, respectively. Two calves infected with pulmonary disease as well diarrhea were cured 8 days after the begining of the therapcutics. 2. Rotaviruses wire detected in the feces of 2 calves, and bovine diarrhea viruses were detected in the 8 calves by the test for serum neutralizing antibody titers, and bovine coronaviruses were also detected in 5 calves. Four calves of the 5 bovine coronavirus infected calves were also infected with bovine diarrhea viruses. 3. Total leucocyte number, total amount of serum protein, and amount of fibringen were slightly increased, while total erythrocyte number, and erythrocyte packed cell volume were slightly decreased. These valucs were statistically not significant. Electrolytes of Na/sup +/, K/sup +/ and Cl/sup -/ were slightly decreased but these values also were not significant. These results indicate that the acupuncture therapeutics arc significantly effective to remove the viral diarrhea in the young calves.
SDS 처리한 브루셀라 항원과 Yersinia enterocolitica 0:9주의 혈청학적 교차반응 연구
임윤규,양기천,이경갑,박전홍,이두식,박용호,강승원,목지원,이영순,Lim, Yoon-kyu,Yang, Ki-chun,Lee, Kyung-kap,Park, Jun-hong,Lee, Du-sik,Park, Yong-ho,Kang, Seung-won,Mok, Ji-won,Lee, Yong-soon 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.1
Brucella abortus cell wall antigen was extracted from Brucella abortus 1119-3 by ultrasonication and followed by sodium dodecyl sulfate(SDS) treatment. In order to confirm whether this preparation is serologically cross reactive with Yersinia enterocolitica 0:9, Western blot analysis with mouse anit-Brucella abortus1119-3 and with mouse anti-Yersinia enterocolitica 0:9 was performed. ELISA results from using those Brucella antigen and Yersinia antigen were assessed whether they had correlation. According to the results of western blot analysis and ELISA, there was no evidence of cross reactivity between the Brucella abortus 1119-3 antigen preparation and Yersinia enterocolitica 0:9. Therefore the SDS treated antigen prepared in this study could be suitably used as specific ELISA antigen without confusion in the interpretation of serological tests for brucellosis in cattle.
김덕환,이교영,이경갑,최희인,오사메 사토시,이찌조 시게루 충남대학교 수의과대학 동물의과학연구소 1994 動物醫科學硏究誌 Vol.1 No.-
In prder to clarify the changes of serum chemical values(AST, CPK and LDH activities)and LDH isoenzyme fractions in frequent occuring some diseases of dairy cows, total 48 cows(40 patients: 18 with downer cow syndrome, 17 with abomasal displacement and 5 with acute mastitis and 8 controls: clinically healthy cows) were examined. The results obtained were as follows: 1. In changes of total serum enzyme activities of patients group, increase of serum AST(P<0.001), CPK(P<0.01) and LDH activities(P<0.01)were observed in groups of downer cow syndrome and abomasal displacement, respectively by comparison with control group. Increase of AST only was found in group of acute mastitis(P<0.05). 2. In LDH isoenzyme fractions, increase of LDH₁(P<0.01), LDH₂(P<0.05), LDH₃(P<0.01), LDH₄(P<0.01) and LDH_5(P<0.01)fractiona was observed in group of downer cow syndrome and also marker increase of LDH4 and LDH5 fractions was noted in this group. The increase of LDH₁, LDH₂ amd LDH₃ (P<0.01)fractions was found in group of abomasal displacement, but group of acute mastitis showed similar result to that of control group.
유기인제에 의한 뇌 손상에 있어서 흥분성 아미노산의 역할
김동식,이봉희,이영재,김소미,김대성,이경갑,조문제,고봉우,박은혜,방성현,진주연,주창완,류기증,박민경 한국농화학회 2001 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.3
This study investigated the role of excitatory amino acid systems in the initiation of organophosphate-induced seizures and brain damages in rats through quantitative in viva microdialysis. Microdialysates were collected from the hippocampus of rat brain, treated with diisopropylfluorophosphate (DFP; 2.67 ㎎/㎏, s.c.) alone, and/or atropine sulfate (15 ㎎/㎏, i.m.) and procyclidine (30 ㎎/㎏, i.m.). The protective effects of atropine, a muscarinic blacker, and/or procyclidine, a N-methyl-D-aspartate and cholinergic antagonist, against DFP were examined. DFP treatment increased the levels of aspartate (Asp) and glutamate (Glu) significantly in the hippocampal persuate with the induction of seizures. Treatment of procyclidine could effectively block the increase of Asp and Glu levels. Atropine treatment showed no significant anticonvulsive effects against DFP-induced seizures. The increases of Asp and Glu levels by DFP were also completely blocked through the combined treatment of atropine and procyclidine. Histopathological findings on the hippocampus confirmed the above results. More effective protection was observed through the treatments of procyclidine alone or of both procyclidine and atropine than atropine alone against DFP-induced brain damage. Procyclidine was shown to be effective in DFP-induced seizures.