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노인순,한정희,김재훈,안병우,Roh, In-soon,Han, Jeong-hee,Kim, Jae-hoon,Ahn, Byeong-woo 대한수의학회 1997 大韓獸醫學會誌 Vol.37 No.4
Suckling piglets and weaned pigs showed anorexia, dehydration, severe abdominal breathing, emaciation and paresis from Oct. 1993. to Nov. 1993. Five 2-week-old piglets were submitted for diagnosis in Kangwon National University. At necropsy, the pin-point well demarcated yellowish white foci were scattered on the surface of the lung, heart, liver, spleen and kidney. Histologically, multifocal areas of necrosis with mononuclear cells infiltration were found in the lung, heart, liver, lymph node, spleen, kidney and small intestine. These lesions tended to be associated with blood vessels. Variable round to ovoid tachyzoites were located at the periphery of the lesions. The organisms were demonstrated as Toxoplasma gondii by immunohistochemical staining method. Ultrastructurally, this parasite was surrounded with parasitophorous vacuole in alveolar macrophage. The parasite was crescent-shaped and $6{\sim}8{\times}1{\sim}2{\mu}m$ in size. It was enclosed by an thick outer membrane and an underlying thin inner membrane. Several club-shaped paired organelles and conoids lay in the cytoplasm at the anterior. Numerous round body and one to several mitochondria were presented in the cytoplasm. Based on the gross findings, histopathology, immunohistochemical and electron microscopic findings, this case was diagnosed as toxoplasmosis in piglets.
국내 분리 porcine circovirus 2의 이유자돈에 대한 병원성 시험연구
노인순,이경우,김재훈,한정희,진영화,Roh, In-Soon,Lee, Kyung-Woo,Kim, Jae-Hoon,Han, Jeong-Hee,Jean, Young-Hwa 대한수의학회 2007 大韓獸醫學會誌 Vol.47 No.2
This study was carried out to investigate the pathogenesis and pathogenicity of the porcine circovirus type 2 (PCV2) Korean isolate from weaned pigs. Twenty four weaned pigs, PCV2, porcine reproductive and respiratory syndrome virus (PRRSV) and porcine parvovirus (PPV) antibodies free, were allocated to 4 groups (n = 6). Six pigs were inoculated intranasally with PCV2 alone, 6 with PCV2 and PRRSV, 6 with the combined PCV2/PRRSV/PPV inoculum, and 6 were remained as a uninoculated negative control. Pigs were killed 3 and 6 weeks after inoculation and tissue samples examined for gross and microscopic lesions and for the presence of PCV2 antigens and nucleic acids. Experimentally inoculated pigs were evaluated for 3 considerations: 1. development of postweaning multisystemic wasting syndrome (PMWS), 2. distribution of viral antigens by immunohistochemistry and polymerase chain reaction (PCR), and 3. cytokine mRNA levels in lymph nodes. Pigs inoculated with PCV2/PRRSV/PPV showed typical clinical signs, gross findings, and histopathologic characteristics of PMWS. In the PCV2/PRRSV/PPV inoculated group, the PCV2 antigen was widely distributed in various parenchymal organs such as brain, spinal cord, tonsil, lymph nodes, lung, heart, liver, kidney, spleen, and peyer's patch. Lymph node mRNA expression of IL-$1{\alpha}$, IL-2R and IL-8 was determined by real-time PCR. The pigs of PCV2/PRRSV and PCV2/PRRSV/PPV inoculation group, the mRNA expression was characterized by a decrease of IL-$1{\alpha}$, IL-2R and IL-8. The decrease of cytokine mRNA represent the state of T cell immuno-suppression in pig, and nicely support the evidence for the impairment of immune system in pigs with PMWS. In conclusion, PCV2 infection and some additional infectious causes such as PRRSV and/or PPV are warranted for the presence of PMWS in weaned pigs in Korea.
강화정,강상철,정지열,조숙희,노인순,이주명,정종태,김정훈,안민찬,배종희,김재훈,Kang, Hwa-Jung,Kang, Sang-Chul,Jung, Ji-Youl,Jo, Suk-Hee,Roh, In-Soon,Lee, Joo-Myung,Cheong, Jongtae,Kim, Jung-Hun,An, Min-Chan,Bae, Jong-Hee,Kim, Jae-Hoon 대한수의학회 2008 大韓獸醫學會誌 Vol.48 No.3
A 10-year-old female Yorkshire terrier with the clinical signs of nasal swelling, epistaxis and nasal discharge was presented to the Veterinary Teaching Hospital in the Cheju National University. Abnormal nasal mass was detected in physical examination and radiographic findings. After surgical excision, the sample of nasal mass was referred to Pathology Department of Veterinary Medicine. Grossly, the mass was soft, friable, and $2.5{\times}4cm$ cm in size. Histopathologically, the mass was composed of mediumsized non-keratinizing columnar to polyhedral cells arranged in anastomosing ribbon and large nest. It has complex in-folding of thick epithelial layers separated by fibrovascular septa. Tumor cells showed characteristic palisading arrangement of columnar cells, and perpendicularly distributed to the basement membrane. The cells had pale basophilic cytoplasm, oval nucleus and one or more nucleoli, and indistinct cellular border. Many tumor cell emboli were presented in lymphatics. Immunohistochemistry revealed that tumor cells were cytokeratin (CK) 19 and CK clone MNF116 positive and but CK7 and CK high molecular weight negative. Based on the gross, histopathologic, and immunohistochemical findings, the mass was diagnosed as transitional carcinoma in nasal cavity. In our best knowledge, this is the first report of transitional carcinoma originated from transitional zone of canine nasal cavity in Korea.
A Case of Natural Mycoplasmosis in Spontaneously Hypertensive Rats
노인순,오승현,박지영,최경철,한정희,진희경,도선길,서준교,오양석,박성준,성제경,Roh, In-Soon,Oh, Seung Hyun,Park, Ji Young,Choi, Kyung-Chul,Han, Jeong-Hee,Jin, Hee-Kyung,Do, Seon Gil,Suh, Jun Gyo,Oh, Yang Seok,Park, Seong-Jun,Seong, Je- The Korean Society of Veterinary Science 2001 大韓獸醫學會誌 Vol.41 No.4
SHR 랫드 4-21 개월령 colony에서 혈청 및 조직학적으로 정기적인 health monitoring을 실시하던 중 ELISA test에서 21 개체 중 12 개체에서 Mycoplasma pulmonis에 대한 항체가 검출되었다. Mycoplasma 양성 개체군의 부검 소견에서 기관지, 세기관지 주변 림프절의 증식과 전형적인 폐 기관지의 병변 소견을 보였다. 혈청학적 검사와 조직학적 소견에서 SHR 랫드 군의 자연적인 Mycoplasm 감염 예로 진단되어 보고하는 바이다. Twenty one Spontaneously Hypertensive Rats(SHR) that were 4- to 21-month-old were examined histopathologically and serologically during a routine health monitoring of the rat colony. The results of the enzyme linked immunosorbent assay(ELISA) for murine pathogens demonstrated that 14 of 21 SHR rats had antibodies to Mycoplasma pulmonis. Histopathologically, the mycoplasma positive SHR rats were observed to have the typical pulmonary lesions which are characterized by the hyperplasia of the lymphoid tissue around the bronchi, bronchioles and vessels. Based on the histopathological findings and serological results, this case was diagnosed as a murine mycoplasmosis of SHR rats.
Porcine Circovirus Infection in Weaned Pigs with Postweaning Multisystemic Wasting Syndrome in Korea
김재훈,노인순,손현주,진영화,황의경,윤경진,Kim, Jae-hoon,Roh, In-soon,Sohn, Hyun-joo,Jean, Young-hwa,Hwang, Eui-kyung,Yoon, Kyoung-jin The Korean Society of Veterinary Science 2003 大韓獸醫學會誌 Vol.43 No.3
Eight nursery to grower pigs exhibiting weight loss and sudden death were diagnosed as postweaning multisystemic wasting syndrome (PMWS) based on the results of gross findings, histopathology, immunohistochemistry, fluorescent antibody test, virus isolation, PCR, serology, and electron microscopy. Groosly, the pigs had a rough hair coats and were severely emaciated. And moot lymph nodes were pale and enlarged. Lungs were not fully collapsed and exhibited 10 to 40% pale red cranioventral consolidation. Histopathologically, typical lymphohistiocytic interstitial to bronchointerstitial pneumonia, chronic lymphadenitis, severe lymphoid depletion, and basophilic intracytoplasmic inclusions were noted in the most lymphoid tissues. Porcine circovirus panicles were observed in the inguinal lymph node of the pigs by electron microscopy. Porcine circovirus type 2 (PCV2) antigens or viral DNAs were detected in the lesions of all pigs using immunohistochemistry or PCR. Two PCV2 were isolated from a homogenate of pooled lung and lymph node in 2 of the 5 pigs. Additionally, antigens of porcine reproductive and respiratory syndrome virus (PRRSV) and Hemophilus (H.) parasuis were also detected by immunofluorescent antibody test. Serologically, 55% of randomly selected sows and fattening pigs was serum antibody positive to PCV2 by an indirect fluorescent antibody (IFA) test and approximately 18 % of animals in the herd were serologically pooitive by the ELISA kit for PRRSV. To our knowledge, this is the first report of PMWS co-infected with PCV-2, PRRS, and H. parasuis in Korea.
Mycobacterium bovis에 의한 소 및 사슴 결핵의 폐 병리조직학적 소견 비교연구
진영화,노인순,이경현,이경우,조윤상,주이석,Jean, Young Hwa,Roh, In Soon,Lee, Kyung Hyun,Lee, Kyung Woo,Cho, Yoon Sang,Joo, Yi Seok 대한수의학회 2008 大韓獸醫學會誌 Vol.48 No.2
Comparative studies of histopathologic lesions from 23 purified protein derivative (PPD) positive cattle, 21 slaughter cattle found with tuberculosis, and 11 tuberculosis-positive elk (Cervus elaphus) were performed. PPD positive cattle did not show specific histopathologic lesions in all 23 heads that were no visible lesion reactor. Slaughter cattle found with tuberculosis revealed microscopically classical granulomatous lesion (tubercle) with central caseous necrosis surrounded by mantle of epithelioid cells and Langhan's giant cells capsuled by connective tissue in lung. Elk was noted with some different lesion patterns with classical granulomatous lesion and suppurative abscesses that was composed of fibrin, degenerated cells without having connective tissue. In addition, many Langhan's giant cells infiltration in alveoli at peripheral lesion were observed in some cases of classical granulomatous lesion and suppurative abscesses.
송아지에서 발생한 Mannheimia haemolytica 폐렴의 병리조직학적 소견
진영화,노인순,이경현,이경우,이희수,Jean, Young Hwa,Roh, In Soon,Lee, Kyung Hyun,Lee, Kyung Woo,Lee, Hee Soo 대한수의학회 2008 大韓獸醫學會誌 Vol.48 No.3
Four 5 month old calves were died after showing respiratory distress after long-distance transportation at winter season. They were diagnosed as fibrinous lobar pneumonia caused by Mannheimia (M.) haemolytica. Grossly, lungs were attached onto the pleura by fibrin, with a rich yellowish fluid in thorax. The cut surface of the lung was showed marbled pattern of the reddish or greyish consolidation and widened interlobular septa by fibrin deposition. Histopathologically, parenchymal necrosis was delineated by a band of the degenerated inflammatory cells, and distended interlobular septa with serofibrinous exudates and vascular thrombosis with alveolar capillaries degeneration and abundant serofibrinous exudates in alveoli. M. hemolytica were isolated from all calves, and bovine viral diarrhea virus and parainfluenza type 3 virus in one calf were detected by RT-PCR. Thus, it was concluded that this case was diagnosed as pneumonic mannheimiosis suggested by complex infection with viruses after long-distance transportation and coldness.
개 전염성 기관기관지염에 대한 modified Live vaccine의 방어효과
박영일 ( Young Il Park ),노인순 ( In Soon Roh ),한정희 ( Jeong Hee Han ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.1
The purpose of this study was to investigate the protective effects of the modified live vaccines against canine Bordetella bronchiseptica(Bb) and canine parainfluenza virus(CPIV) in puppies by nasal administration. A total of 24 puppies were classified as 3 groups consisting of 8, and each one was divided into two subgroups; vaccinated (n=4) and unvaccinated(n=4). Group I, group II and group III were challenged with Bb, CPIV, and Bb+CPIV, respectively. In group I vaccinated puppies(n=4) were experi-mentally challenged with Bb 2 weeks after vaccination and unvaccinated puppies(n=4) were experimentally challenged with Bb alone. The same methods of the above were applied for group II and group III. We carried out several studies including serum tests, isolation, and histopathological examination. The vaccinated puppies showed higher antibody titers than unvaccinated puppies and the titer sustained during the experi-mental period. In the isolation test, recovery period was shorter in the vaccinated subgroup than in the other. In clinical signs, the unvaccinated puppies showed the typical signs of tracheobronchitis (coughing, nasal and occular discharge), but another subgroup showed delayed incidence and mild clinical signs. The typical gross lesions and histopathological findings were observed in the unvaccinated puppies. In immuno-histochemical findings, the vaccinated puppies showed little intensive in reaction for CPIV antigen than the other. It could be concluded that intranasal vaccination of modified live Bb and CPIV vaccine to puppies is effective to prevent canine infectious tracheo-bronchitis.