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표준입도 분산조건에서 나노입자의 THP-1 세포에 대한 독성영향
이한들(Handule Lee),박주영(Juyoung Park),박광식(Kwangsik Park) 대한약학회 2017 약학회지 Vol.61 No.2
Effect of nanoparticles on cellular toxicity can be influenced by size and/or size distribution of the tested nanoparticles. Therefore, it is necessary to fulfill the requirement of standardized dispersion when toxicity potencies of different types of nanoparticles are compared. In this study, probe-sonicator calibration for delivered acoustic power was performed according to the NANoREG protocol for standardized size-distribution of test nanoparticles including silica nanoparticles (SiNP), ceria nanopaaticles (CeNP), titania nanoparticles (TiNP), silver nanoparticles (AgNP) and zinc nanoparticles(ZnNP). Based on the protocol, the increase of water temperature by sonication power was monitored and Pac value was obtained. When the time of 8 minutes and 25% amplitude calculated from the Pac value was applied to the sonication of silica nanoparticles, size distribution reached to the effective level, 210 nm < size < 270 nm, Polydispersity index(PDI) < 0.46. The same acoustic power used in the dispersion of silica nanoparticles was applied to the other test nanoparticles, and cytotoxicity test was performed by MTS assay in cultured THP-1 cells treated with nanoparticles for 24 hrs. As results, Lethal Concentration 50% (LC50) of silver nanoparticles was 78.44 ppm, which was most toxic among the test nanoparticles. The toxicity compared by the LC50 seemed to be AgNP > SiNP > TiNP > ZnNP > CeNP.
In vitro 독성시험에 적용할 나노입자의 입도분산 표준화 기법
박주영,이한들,박광식,Park, Juyoung,Lee, Handule,Park, Kwangsik 대한약학회 2016 약학회지 Vol.60 No.4
NANoREG is a program for a common European approach to the regulatory testing of manufactured nanomaterials. NANoREG has an objective to provide answers and solutions to the questions and requirements from regulatory authorities. For this, NANoREG is generating physicochemical and toxicological data by interdisciplinary approaches and we have joined the program since January, 2015. According to the NANoREG program, it is necessary to fulfil the requirement of nanomaterial dispersion protocol before starting toxicity tests. So, we performed probe-sonicator calibration of delivered acoustic power based on the protocols provided by NANoREG. As results, the amplitude and time for sonication was obtained for the proper size distribution of standard nanomaterial, silica nanoparticles NM 200. All data we obtained belonged to the effective level and quality of the dispersion, 210 nm < Size < 270 nm, PDI < 0.46. Sized distributions of other nanomaterials including titania, zinc, ceria, and silver were also measured with the same amplitude and time for sonication. However, a few problems were raised to the procedure of calibration methods and issues on the protocol were discussed in this study.
Juyoung Park(박주영),Handule Lee(이한들),Kwangsik Park(박광식) 환경독성보건학회 2021 한국독성학회 심포지움 및 학술발표회 Vol.2021 No.5
Some chemicals commonly used in personal care products, household items, food vessels, cosmetics, and other consumer products are potentially harmful, and several reviews of epidemiological studies have suggested the associations between the chemical exposure from consumer products, and respiratory diseases, skin sensitization, and reproductive problems. Therefore, risk assessment is essential for management of consumer products safety. Necessarily, the estimation of human exposure is an essential step in risk assessment, and the absorption rate of those chemicals via the gastrointestinal tract, respiratory tract, and skin are very critical in determining the internal dose of the exposed chemicals. In this study, parallel artificial membrane permeability assays (PAMPA) for the gastrointestinal tract and skin were performed to evaluate the permeability of parabens, bisphenols, isothiazolinones, and phthalates. Lipid solubility of test chemicals indicated by log P values was shown as the most critical factor and showed a positive association with the permeability of parabens, bisphenols, and isothiazolinones in PAMPA assay. However, phthalates showed a reverse-association between lipophilicity and permeability. The permeability of all the tested chemicals was higher in the gastrointestinal tract membrane than in the skin membrane. The pH in donor solution did not show significant effects on the permeability in all the chemicals, except the chemicals with a free hydrophilic moiety in their chemical structures.