Isoeugenol의 넙치(Paralichthys olivaceus)에 대한 진정, 마취 및 독성효과 검토

신기욱,신용승,김영림,이은영,양향희,팔락샤,허남응,연성찬,이희천,오명주,조성준,강승원,정태성,Shin, Gee-wook,Shin, Yong-seung,Kim, Young-rim,Lee, Eun-young,Yang, Hyang-hee,Palaksha, K.J.,Huh, Nam-eung,Yeon, Seong-chan,Lee, Hee-chun,Oh, Myung- 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.2

Olive flounder (Paralichthys olivaceus) is the major mariculture fish in Korea. The annual aquaculture production of olive flounder in Korea during the period of 2003 was 300,000 ton (2002 Statistics, Ministry of Maritime Affairs & Fisheries, Korea Government). Anesthetics is very necessary in aquaculture to minimize stress and damage during harvesting, grading, transportation, spawning induction and handling to fish. In the present study, isoeugenol as new anesthetic in marine fish, especially olive flounder, was examined to know the efficacy and proper concentration. As a result, olive flounder adult was exhibited sedation at 5 ppm at 10 and $15^{\circ}C$, and 7.5 ppm at $20^{\circ}C$, respectively. Anesthesia was required at least 10, 7.5 and 10 ppm at $10^{\circ}C$, $15^{\circ}C$ and at $20^{\circ}C$, respectively. In case of fry, the effect of sedation was observed from 2.5 ppm at $10^{\circ}C$ and 5 ppm at 15 and $20^{\circ}C$, respectively. Anesthesia was observed from 2.5 ppm at $10^{\circ}C$, 5 ppm at 15 and $20^{\circ}C$, respectively. In acute toxicity test, it was impossible to explore $LD_{50}$ with the concentration of isoeugenol adult at $15^{\circ}C$ used, but over immersion volume of 15 ppm at $15^{\circ}C$ was observed mortality in fry. Based on the present study, isoeugenol was identified as a safe and active anesthetic to olive flounder.

이차원전기영동을 이용한 Escherichia coli O157:H7 균주간 항원 Spot의 비교

안영창,신기욱,신용승,이응구,이형준,박미림,김영림,정태성,김곤섭,김용환,Ahn, Yeong-Chang,Shin, Gee-Wook,Shin, Yong-Seung,Lee, Eung-Goo,Lee, Hyoung-Jun,Park, Mi-Rim,Kim, Young-Rim,Jung, Tae-Sung,Kim, Gon-Sup,Kim, Yong-Hwan 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.2

Proteomics is an emerging powerful tool in studying protein expression and function. At present study, proteomics was employed to evaluate the antigenicity among Escherichia coli O157:H7 strains using 2-dimensional gel electrophoresis (2-DE) and immunoblotting, SDS-PAGE and immunblotting analysis revealed no big differences among E coli O157:H7 strains. 2-DE analysis, however, revealed common antigens as well as specific antigens. The immunoblotting analysis revealed 20 common antigenic spots among E coli O157:H7 strains. In addition, there were 3 and 13 spots as common antigens between ATCC 43894 and KSC 109, and between ATCC 43894 and ACH 5, respectively. Antigenic spots specific for individual strain were also identified as 15, 8 and 22 for ATCC 43894, ACH 5 and KSC 109, respectively. The common antigens would be useful by employing either vaccine development or diagnosis marker, or both, whereas the specific antigens of individual strains would be applicable for epidemiological study. This study suggest that proteome analysis, representative as 2-DE, is valuable tool in exploring the E. coli antigenicity.

야생 멧돼지에서 돼지신충(swine kidney worm) 감염 예

서명득,신기욱,김종섭,곽수동,김순복,연성찬,Suh, Myung-deuk,Shin, Gee-wook,Kim, Chong-sup,Kwak, Soo-dong,Kim, Soon-bok,Yeon, Seung-chan 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.1

This is the first case report of Stephanurus dentatus infection of a feral pig in Korea. In late April, 2000, a weakened feral pig was caught by blow gun from a very low level mountain near the Gyeongsang National University. We autopsied the feral pig in the laboratory of veterinary anatomy at the College of Veterinary Medicine. A total of 27 adult parasites, 11 females and 16 males, and numerous eggs were observed from the cysts formed in the perirenal tissues and ureters. The average size of males was $25.1{\pm}3.2mm$ long and of the females was $34.2{\pm}2.9mm$. The worms were stout, the females being about 2mm broad, and the internal organs were partly visible through the cuticle. The shape of thin-shelled eggs found in the cysts of perirenal tissues and ureter was ellisoidal and oval, and measured $40{\sim}65{\times}90{\sim}115{\mu}m$. The adult parasites were found in cysts which varied from 0.6 to 4cm in diameter, each cyst usually containing a pair of adult worms embedded in green pus. The ureter was thickened and almost occluded, with consequent hydronephrosis.

Polymerase chain reaction을 이용한 실험적 감염 돼지의 혈액과 조직으로부터 Toxoplasma gondii 검출

신명득,신기욱,Suh, Myung-deuk,Shin, Gee-wook 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.1

This study was conducted to detect the toxoplasma specific-DNA in circulating blood and organs collected from slaughtered pigs at slaughtering house and experimentally infected pigs with Toxoplasma gondii tachyzoites by polymerase chain reaction(PCR), and also PCR was applied to diagnose for acute phase of swine toxoplasmosis as a newly developed diagnostic test. The sensitivity of oligonucleotide primer, T-1 & T-2, designed from toxoplasma B1 gene amplification method was compared with Tp parasite detection by mouse inoculation(MI). On the other hand, latex agglutination test(LAT) was conducted to detect the serum antibodies comparing with the detection of toxoplasma by PCR and MI. The results obtained were summarized as follows. PCR was able to determine at the lowest level of $10^0/ml$ T. gondii in blood samples which were blended with a serial diluted T gondii in vitro. On the other hand, $10^2/5g$ of T gondii could detect from a variety of tissues including lung, diaphragm, liver, heart, spleen and brain in vitro. The primer was proved to specifically determine T gondii in blood and tissues in vitro but it did not detect Neospora caninum used as a negative control. DNA of T. gondii was effectively extracted by freezing, thawing and grinding twice both tissues mixed with T gondii in vitro and in experimentally infected pig's tissues. PCR detected specific DNA in the blood of experimentally infected pigs at 108 hrs and 120 hrs post-infection, it was the same time that the pigs showed fever and parasitaemia. In case of tissue, specific DNA was, however, detected only lung from experimentally infected pigs. Even though the duration of acute phase was from 3 to 7 days post-infection, but the latex agglutination test (LAT) results appeared from 8 days post-infection. A comparison of sensitivity in determining T gondii in blood samples between PCR and MI, PCR positive rate ranged from 25 to 33.3%, but that of MI covered from 75 to 100%.

원저 : Propolis의 어류 병원성 세균에 대한 in vitro 항균 효과

허강준 ( Gang Joon Heo ),원태경 ( Tae Gyeong Won ),신기욱 ( Gee Wook Shin ) 한국가축위생학회 2015 韓國家畜衛生學會誌 Vol.38 No.2

The present study was to investigate in vitro antimicrobial effects of propolis against six different fish bacterial pathogens, Aeromonas hydrophila, Edwardsiella tarda, Vibrio vulnificus, V. parahaemolyticus, A. salmonicida subsp. masoucida, A. salmonicida subsp. salmonicida) using minimum inhibitory concentration (MIC) and minimum bacteriocidal concentration (MBC) tests. In the results, propolis exhibited antimicrobial activity against all bacteria used in the present study, but there was no marked difference in bacterial species except Vibrio species. Collectively, propolis was thought to be an usefulness antimicrobial substance for controlling bacterial diseases in the fish industry.

어류의 병원성 세균에 대한 curcumin의 항균효과

허강준 ( Gang Joon Heo ),강진휘 ( Jin Hui Kang ),신기욱 ( Gee Wook Shin ) 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.4

The present study was to investigate anti-microbial effects of curcumin on major bacterial pathogens for farmed fish, such as Aeromonas hydrophila, A. salmonicida subsp. masoucida, A. salmonicida subsp. salmonicida, Edwardsiella tarda, Vibrio vulnificus, V. paraheamolyticus using disk diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests. In disc diffusion test, curcumin exhibited concentration-dependent antimicrobial activities to all bacteria pathogens used in the study. Antimicrobial effects of curcumin was found differently depending on bacterial species when determined by MIC or MBC tests. For examples, E. tarda and A. hydrophila was respectively the most sensitive bacterium for bacteriostatic and bacteriocidal effect of curcumin. Collectively, curcumin could be a potential natural drug for controlling pathogenic bacteria in the aquaculture industry.

이스라엘 잉어( Cyprinus carpio)에서 Aeromonas veronii 감염증의 증례 phylogenetic analysis와 항생제 내성

이승원 ( Seung Won Yi ),유명조 ( Myung Jo You ),이해범 ( Hae Beom Lee ),신기욱 ( Gee Wook Shin ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.3

We reported an outbreak of Aeromonas (A.) veronii responsible for ulcerative dermatitis in Israeli carp (Cyprinus carpio). The major clinical signs were darkening body, abdominal extension, exophthalmos and severe ulcerative necrosis in the skin. The necropsy showed yellowish ascites, necrosis in liver and enlargement of kidney and spleen in the morbid fish. In blood agar for culturing bacteria, three different colonies were identified as A. veronii, Plesimonas shigelloides and Shewanella putrefaciens by phylogenetic identification using 16S rRNA or gyrB gene sequences. A. veronii was the most dominant species among them and was resistant to ampicillin, nalidixic acid and oxytetracycline.

In vitro에서 미색류 껍질로부터 추출한 콘드로이틴황산의 기능성 화장품 소재로서의 가능성

김영림(Yong-Rim Kim),안삼환(Sam-Hwan Ahn),최병대(Byeong-Dae Choi),강석중(Seok-Joong Kang),신기욱(Gee-Wook Shin),오명주(Myung-Joo Oh),정태성(Tae-Sung Jung) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.4

CCD 1108Sk 를 이용한 세포 활성을 조사한 결과 Sigma사 제품(Bovine Trachea에서 얻어진 콘드로이틴황산)은 80 ㎍/mL에서 높은 활성을 보였으나, 미더덕 및 멍게로부터 추출한 콘드로이틴황산은 400~600 ㎍/mL에서 높은 활성을 보였다. 이러한 활성의 차이는 순도의 차이에 기인하거나 서로 다른 구조에 기인한다고 여겨졌다. 그러나 MDD F1은 1,000㎍/mL에서 활성이 증가하는 모습이 보였지만 MDD F2는 미약하게 증가하는 모습을 보여 fraction들간에 차이가 있음을 보였다. A 431 주화세포를 이용한 실험에서는 Sigma사 제품과 미더덕과 멍게에서 추출한 crude 콘드로이틴황산의 경우는 CCD 1108Sk에서 보여준 결과와 비슷하였으나, MDD F1과 F2는 모두 600~1,000 ㎍/mL 사이에서 최고의 활성을 보였다. Collagen 합성에 있어서는 bovine trachea, MDD F1과 F2는 조사 범위에서는 특이 활성을 보이지 않았다. 그러나 멍게에서 추출한 콘드로이틴황산의 경우는 100 ㎍/mL에서, 미더덕에서 추출한 콘드로이틴황산의 경우는 50 ㎍/mL에서 CCD 1108Sk 세포에서 각각 최고의 활성을 보였다. 그러나, A 431 세포에서는 검사한 모든 물질에 대하여 특이 반응을 확인할 수 없었다. 자외선 손상에 대한 회복능 조사는 세포 상층액과 세포 자체가 생성한 LDH를 조사한 결과, 멍게와 미더덕에서 추출한 콘드로이틴황산의 경우는 400 ㎍/mL에서 CCD 1108Sk 세포의 상층액에서 높은 LDH를 보였으나, 다른 물질의 경우 조사범위에서는 반응을 보이지 않았고, 세포 자체에서도 LDH는 조사 물질간 큰 차이를 나타내지는 않았다. 멍게와 미더덕에서 추출한 crude 콘드로이틴황산의 경우는 400 ㎍/mL에서 최고의 활성을 보였다. 전체적으로 멍게에서 추출한 콘드로이틴황산이 미더덕에서 추출한 콘드로이틴황산의 경우보다 세포활성이 높게 나타났다. 그렇지만 두 해양생물로부터 추출한 콘드로이틴황산은 화장품 원료로서의 사용이 가능함이 판명되었다. With the aim of using a cosmetic material, chondroitin sulfates extracted from midduck tunics (Styela clava) and munggae tunics (Halocynthia roretzi) were examined in vitro with two cell lines for cell toxicity, collagen synthesis, cell growth and recovery ability after U.V. irradiation. Cell toxicity test with A 431 and CCD 1108Sk was able to observe high activity between 400 and 600 ㎍/m while standard chondroitin sulfate (CS) purchased from Sigma was showed at 80 ㎍/mL. Even fraction 1 and 2 collected from chondroitin sulfates originated from midduck appeared having the highest activity between 600 and 1000 ㎍/mL, but slightly lower compared to crude chondroitin sulfates from both mideduck and munggae. In cell growth examination, it was not able to find significant differences between chondroitin sulfates used. Both crude chondroitin sulfates were exhibited the highest activity for two cell lines except that of mideduck which was showed activity for CCD 1108Sk. CS, fraction 1 and 2 from midduck were not able to demonstrate a significant activity in collagen synthesis. On the contrary, crude chondroitin sulfates from both munggae and midduck were showed the highest activity at 100 and 50 ㎍/mL with only CCD 1108Sk. The recovery ability after U.V. irradiation with crude chondroitin sulfates from both munggae and midduck were showed high activity at 400 ㎍/mL with CCD 1108Sk and A 431. But there were no activity observed in fractions examined. As a consequence, the crude chondroitin sulfates from both munggae and midduck might not only be available as a cosmetic material but also useful for increasing some activity by blending properly.