리베스 ( Ribes nigrum L. var. ' Rosenthals Langtraubige Schwarze ' ) 식물의 화아분화에 (花芽分化) 관한 연구

양덕조 (Deok Cho Yang) 한국식물학회 1984 Journal of Plant Biology Vol.27 No.3

N/A

장록에서 유도한 Betalain 합성 세포주의 액체 배양과 모상근의 특성

양덕조(Deok Cho Yang),이성종(Sung Jong Lee),윤길영(Kil Young Yun),강영희(Young Hee Kang) 한국생물공학회 1993 KSBB Journal Vol.8 No.1

Binary Vecotor System을 이용한 당근 (Daucus carota)세포의 형질전환

양덕조(Deok-Cho Yang),이성택(Sung-Taick Lee) 한국생물공학회 1990 KSBB Journal Vol.5 No.3

인삼엽요병에서 Active Oxygen Species (^1O_2, O_2^-, H_2O_2$)가 Chlorophyll Bleaching에 미치는 영향 및 방제대책에 관한 연구

양덕조,김명원,채쾌,김명식,Yang, Deok-Cho,Kim, Myoung-Won,Chae, Quae,Kim, Myeong-Sik 고려인삼학회 1989 Journal of Ginseng Research Vol.13 No.1

In order to determine the specific active oxygen species directly related to chlorophyll bleaching in the leaf-burning disease, we investigated the effects of singlet oxygen (1O2), superoxide radical (O2-), and hydrogen Peroxide (H2O2) on isolated chloroplast suspension and leaf discs from Panax ginseng C.A. Meyer. When the singlet oxygen was added to the chloroplast suspension, the chlorophyll and carotenoid contents were decreased by more than 809), similar to treatment with high light intensity (100 KLux). We assumed that the conversion of dioxygen (O2) produced either in photolysis or in breakdown of hydrogen peroxide to singlet oxygen resulted from photorespiration. On the basis of these experiments , :he inhibitory effects of active oxygen scavengers propylgallic acid (PGA), 2,5-ditetrabutyl hydroquinon (DBH), sodium pyrosulfate (SPS), and ascorbic acid (ABS) were examined. In chloroplast suspension all four scavengers inhibited chlorophyll bleaching by more than 75fl , and in the leaf discs the inhibition rates of SPS, PGA and ABS were 46%, 51%, and 96% respectively.

인삼 틸라코이드에서 Singlet Oxygen($^1$O$_2$) 생성에 미치는 전자전달계의 영향

양덕조,채쾌,이성종,김용해,강영희,Yang, Deok-Cho,Chae, Quae,Lee, Sung-Jong,Kim, Yong-Hae,Kang, Young-Hee 고려인삼학회 1990 Journal of Ginseng Research Vol.14 No.1

In order to Investigate the mechanism of the leaf-burning disease of ginseng (Panax ginseng C.A. Meyer), studies on the generation of singlet oxygen (1O2) and the photooxidation of the pigments were carried out in comparison with the ones of soybean (G1ycine max L). The studies were mainly focalized on the effects of light intensity, light intensity, inhibitor and electron donor/acceptor of the Photosynthetic electron transport system. When we measured the amounts of 1O2 generated in the thylakoids of ginseng and soybean by the irradiation of light (300 w/m2) as a function its time. It was identified that a higher amount of 1O2 was formed in the ginseng thylakoid than the case of soybean. A generation ratio of lO2 between ginseng and soybean sltbstantially identical in the range of light intensities 50∼150w/m2 However much higher amount of 1O2 was generated in ginseng by irradiation of strong intensity of light (200 500w/m2). Wave length dependency on the generation of 1O2 and the pigment photooxidation was observed on ginseng thylakoids; red light (600-700 nm) gave a maximum effect in the contrast with blur green light (400-60 nm). When the ginseng thylalioid was treated with the electron donor (Mn2+) and acceptors (DCPIP, FeCy) of the photosynthetic electron transport system. a drastic inhibition of 1O2 generation was observed. However, treatment with its inhibitors (DCMU, KCW) activated 1O2 generation. An interesting fact that an electron donor or acceptor of the photosystem II(P680) Inhibited 1O2 generation, suggests an intimate relationship between 1O2 generation and photosystem II.

국내 자생 그늘쑥 (Artemisia sylvatica MAX) 모상근의 대량배양을 위한 최적조건

신선희,양덕조,Shin, Sun-Hee,Yang, Deok-Cho 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.1

본 실험은 그늘쑥을 Agrobacterium을 통해 형질전환체인 모상근을 유기하고 모상근의 대량배양을 위해 모상근이 가장 잘 자라는 생장 조건을 찾기 위해 수행되었다. 실험 결과를 종합해 보면 선발된 그늘쑥 모상근 세포주의 대량 생산을 위한 최적 배지는 MS 기본 배지임을 확인하였다. 또한 최적의 havesting time 모상근의 성장률이 좋고 갈변과 배지의 미생물 오염도가 적은 4주가 적당한 것으로 판단된다. 그리고 광 처리 하에서 모상근을 배양하는 것보다는 암상태에서 모상근을 키우고 pH는 5.8 정도가 탄소원은 sucrose로 3.5%가 모상근 을 대량배양시 최적조건임을 확인되었다. 미량원소인 Fe$^{2+}$은 0.1 mM을 처리하는 것이 모상근의 성장을 촉진하였으며 spermine과 spermidine은 각각 10 mM와 100 mM로 처리하는 것이 모상근의 성장을 높일 수 있다. 앞으로 연구는 이 결과를 토대로 모상근 대량 배양을 위한 scale up조건과 bioreator의 설계에 관한 연구가 필요하다. This research aims the production of anti-tumor substances through in vitro culture of hairy roots transformed by Agrobacterium rhizogenes in Artemisia sylvatica MAX and the effect of culture conditions on optimum growth of hairy roots. We investigated the optimum medium, pH, carbon source, sucrose, light, Fe and polyamine conditions of various lines of hairy roots (NK3, NK4, YX. NK3-10) induced from Artemisia sylvatica to increase the optimum growth of hairy roots. MS medium was the best for optimum growth of hairy root clone, NK3-S10. The optimum culture period was 4 weeks for NK3-S10. The optimum sucrose concentration was 3.5%. The optimum concentration of FeSO$_4$, spermine and spermidine was 0.1 mM, 10 mM and 100 mM, respectively.

칼슘이온 influx 에 미치는 Cholesterol 과 Phytosterol 의 효과

여종원,김학룡,양덕조,채쾌 ( Jong Won Yeo,Hak Yong Kim,Deok Cho Yang,Quae Chae ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.1

There is a report that cholesterol and/or some sterols give a positive effect on flowering of plants. Under assumption that the phenomenon can be induced by the enhancement of Ca^(2+) influx through the interaction between cholesterol and the plasma membrane, we carried out the experiment to determine whether the change of the cytosolic Ca ^(2+) concentration could be induced by binding of these compounds to the plasma membrane of oat protoplast. The results show a linear increase of the cytosolic Ca^(2+) concentration as a function of cholesterol concentrations added to the protoplast. However, other phytosterols (sitosterol and stigmasterol) were ineffective. The cytosolic Ca^(2+) concentration was determined by using a calcium specific fluorescent indicator Quin II. Red light effect on Ca^(2+) influx was also investigated in the normal protoplast and the cholesterol bound one. In comparision with the control, a higher influx of Ca^(2+) in the case of the cholesterol bound protoplast was observed when irradiated with red light. However, when the Ca^(2+), influx was blocked by 2 mM EGTA, the cytosolic Ca^(2+) concentration was not changed by cholesterol addition, but slightly increased by irradiation with red light.

⁶⁰Co γ 를 조사한 인삼모상근 돌연변이 세포주의 생장과 Ginsenoside의 생산

최규명,권정희,반성희,양덕조,Choi, Kyu-Myoung,Kwon, Jung-Hee,Ban, Sung-Hee,Yang, Deok-Cho 고려인삼학회 2002 Journal of Ginseng Research Vol.26 No.4

본 연구는 인삼(Panax ginseng C. A. Meyer)의 모상근에 $^{60}$Co ${\gamma}$-ray의 조사에 따른 영향을 구명하고자 수행되었다. 0.5~4 Krad의 다양한 방사선을 조사한 결과 모상근은 3Krad이상에서 생장이 억제되었다. 2 Krad이하의 방사선을 조사한 모상근의 정단을 제거한후 자라난 측근을 하나의 세포주로 하여 생장과 형태가 다양한 206개의 모상근 세포주를 선발하고 1/2 MS 호르몬 무첨가 배지에서 배양하였다. 206개의 세포주 중 생장이 우수한 10개의 세포주를 선발하였는데 특히 대조구에 비해 ${\gamma}$-GHR 70과 ${\gamma}$-GHR 94의 생장이 각각 34.5%, 44.7% 으로 가장 높음을 확인하였다. 생장이 우수한 10개 세포주의 형태적 특징을 주근의 굵기와 측근의 돌기형성, 측근의 생장정도에 따라 관찰하였던 바, 생장이 우수한 세포주는 1차근이 가늘며 왕성한 생장을 나타남을 확인하였다. Ginsenoside의 함량에 있어서 생장이 우수한 10개의 세포주 중 8개의 모상근 세포주가 대조구보다 함량이 높았으며 특히 ${\gamma}$-GHR 59와 ${\gamma}$-GHR 94는 각각 19%와 16.9%로 가장 높은 함량을 나타내었다. 따라서 ${\gamma}$-ray를 조사한 모상근 세포주 중 총 ginsenoside 함량이 우수하고 생장 또한 우수한 세포주로 ${\gamma}$-GHR 70, ${\gamma}$-GHR 94를 선발하였다. 특정 ginsenoside의 함량별로 보면 항암에 효과적인 Rb$_2$는 ${\gamma}$-GHR 57가 7.6%, Rc는 ${\gamma}$-GHR 69가 16.2%의 함량 증가를 나타내어 특정 ginsenoside 생산에 효율적일 것으로 보인다. 돌연변이와 관련된 유전자(VNTRP)분석은 현재 진행 중에 있다. Study was performed to know the effects of Panax ginseng C.A. Meyer hairy root due to $^{60}$ Co ${\gamma}$-ray irradiation. We irradiated the hairy roots under the various $^{60}$ Co ${\gamma}$-ray; 0.5~4 Krad. The growth of hairy roots is inhibited over 3 Krad treatment. The lateral roots are used as a cell line after removing the apical meristem of hairy roots irradiated below 2 Krad. We selected 206 hairy root cell lines having various different growth rates and forms, and incubated in the 1/2 Murashige & Skoog(MS) medium in the absence of hormone. We selected 10 out of 206 showing superior growth. Among those, ${\gamma}$-GHR 70 and ${\gamma}$-GHR 94 showed higher growth; 34.5, 44.7%, respectively. We observed shapable, sizable characteristics according to the width of the primary roots, the process formation of the lateral roots, and the growth of lateral roots. The discriminable cell line showed that primary root is thinner, and has a vigorous growth. 8 out of 10 had much more contents than control in the aspect of the ginsenoside. ${\gamma}$-GHR 59 and ${\gamma}$-GHR 94 showed higher contents; 19, 16.9%, respectively. Therefore, we selected ${\gamma}$-GHR 70, ${\gamma}$-GHR 94 as a superior cell line in the aspect of ginsenoside contents, and growth among those irradiated by ${\gamma}$-ray. According to content of ginsenoside, Rb$_2$ effective in anticancer has 7.5% of ${\gamma}$-GHR 59. Rc, also effective in anticancer showed 16.2% content increasement of ${\gamma}$-GHR 69. It is thought that those lines will be effective in manufacturing ginsenoside. Gene analysis (VNTRP) related to the mutation is in progress.

송이(Tricholoma matsutake)에서 분리된 균사 배양액의 Tyrosinase 억제효과

우현정(Hyun-Jung Woo),양덕조(Deok-Cho Yang) 한국생물공학회 2003 KSBB Journal Vol.18 No.1

煙草 crown gall tumor 및 teratoma의 出現樣相

Kwang Tae Choi(崔光泰),Deok Chun Yang(梁德春),Deok Cho Yang(梁德祚) 한국육종학회 1986 한국육종학회지 Vol.18 No.2

These studies were carried out to clarify the formation pattern of crown gall tumor and teratoma in Nicotiana tabacum L. and the effect of arginine on the growth of tumor callus. Infection of N. tabacum var. NC 2326, NC 82, and BY 4 by A. tumefaciens C 58 led to the crown gall tumor, and teratoma and secondary tumor were also observed. The tobacco tumor callus was formed profusely on the phytohormone free medium. And shoots were, also, formed from tobacco tumor callus cultured on the phytohormone free medium. As for the effect of a arginine, it was found that arginine did not arfect the growth of tobacco tumor callus.