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- 저자 : Chang-Eun Lee(이창은) (검색결과 89 건)
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이창은(Chang Eun Lee),황인경(In Kyoung Hwang),정영일(Young Il Chung),정기선(Key Sun Jung) 한국병원경영학회 2000 병원경영학회지 Vol.5 No.1
This study was designed to find out the relations between the major investment decision-making behaviors and profitability of the hospital. A total of 57 hospitals were analyzed on this study. The major findings were as follows ; 1. Among the types of the investment decision-making, major factors affecting the profitability were where the top management belongs among the defender, analyzer, prospector, and reactor type. Other factors were whether or not hospital analyzed which is more economical between the purchase by cash and lease of the medical equipment and whether or not hospital changes the decision before the actual investment. 2. Among the types of the investment decision-making, major factors affecting the financial structure and efficient operation of the assets were ranking of the priority and whether or not hospitals can get enough revenue and cash flow when hospitals have to borrow a big amount of fund from outside. 3. Among the financial indices regarding the financial stability, major factor affecting the profitability was fixed assets to long-term capital. Other factors affecting the financial structure and efficient operation of the assets were value added to medical equipment, normal profit to medical equipment, liability to total assets, current ratio, value added to payroll expenses. 4. Investment decision-making behaviors are partially influencing on the financial structure and efficient operation of the assets. However it was proved that the profitability was the most influencial factor than other factors related with the operation of the hospital. 5. To improve the irrational investment decision-making behaviors strategic management system should be introduced, and the top management`s investment decision-making style should be changed from reactor and analyser styles to prospector and reactor ones.
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신규 한천분해세균 Maribacter sp. SH-1의 분리 및 효소 특성조사
이창은 ( Chang Eun Lee ),이솔지 ( Sol Ji Lee ),이동근 ( Dong Geun Lee ),이상현 ( Sang Hyeon Lee ) 한국미생물생명공학회(구 한국산업미생물학회) 2016 한국미생물·생명공학회지 Vol.44 No.2
본 연구에서는 신규 해양성 한천분해균을 분리하고, 이 균주가 생성하는 한천분해효소의 특성을 조사하였다. 경상남도 남해군 미조면 연안에서 채취한 해수를 Marine agar 2216 배지에 도말하여 한천분해세균을 선별하였다. 선택된 한천분해균주는 16S rDNA 염기서열분석을 통해 Maribacter속 세균과 99% 유사하여 Maribacter sp. SH-1으로 명명하였다. 세포외로 분비되는 agarase는 Maribacter sp. SH-1 균주 배양액에서 획득하였으며, 이를 이용하여 특성을 조사 하였다. Maribacter sp. SH-1 균주의 한천분해효소는 20, 30, 40, 50 및 60℃에서 각각 56, 62, 94, 100, 8%의 상대활성을 나타냈으며, pH 5, 6, 7 및 8에서 각각 15, 100, 60, 21%의 상대활성을 나타냈다. 세포외 agarase는 50℃, pH 6인 20 mM Tris-HCl buffer를 사용하는 조건에서 최대활성(231 units/l)을 보였다. 한천이 sol 상태로 존재하는 50℃에서 최적활성을 보여 이 효소는 응용 가능성이 높다고 할 것이다. 효소 활성은 20, 30 및 40℃에서 30분 동안 열처리하였을 때 약 90% 이상의 상대활성을 보였다. TLC 분석 결과, Maribacter sp. SH-1 균주의 한천분해효소는 한천올리고당인 neoagarohexaose (34.8%), neoagarotetraose (52.2%) 및 neoagarobiose (13.0%)를 생성하는 것으로 보아 β-agarase로 확인되었다. 따라서 Maribacter sp. SH-1 균주와 이 균주가 생산하는 β-agarase는 보습효과, 미백효과, 세균성장 억제 혹은 전분노화 방지 등의 기능을 가지는 한천올리고당의 생산에 유용할 것으로 기대된다. In this study, we isolated a new agar-degrading marine bacterium and characterized its agarase. An agardegrading marine bacterium SH-1 was isolated from seawater, collected from the seashore of Namhae in Gyeongnam province, Korea, and cultured in marine agar 2216 media. It was identified as Maribacter. sp. SH-1 by phylogenetic analyses, based on 16S rRNA gene sequence. The extracellular agarase was extracted from culture media of Maribacter sp. SH-1 and characterized. Its relative activities were 56, 62, 94, 100, and 8% at 20, 30, 40, 50, and 60°C, respectively, whereas 15, 100, 60, and 21% relative activities were observed at pH 5, 6, 7, and 8, respectively. Its extracellular agarase exhibited maximum activity (231 units/l) at pH 6.0 and 50°C, in 20 mM Tris-HCl buffer. Therefore, this agarase would be applicable as it showed the maximum activity at the temperature at which the agar is in a sol state. Furthermore, the agarase activities remained over 90% at 20, 30, and 40°C after 0.5 h exposure at these temperatures. Thin layer chromatography analysis suggested that Maribacter sp. SH-1 produces extracellular β-agarase, as it hydrolyzes agarose to produce neoagarooligosaccharides, such as neoagarohexaose (34.8%), neoagarotetraose (52.2%), and neoagarobiose (13.0%). Maribacter sp. SH-1 and its β-agarase would be useful for the production of neoagarooligosaccharides, which shows functional properties, like skin moisturizing, skin whitening, inhibition of bacterial growth, and delay in starch degradation.
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이창은,윤종섭,문영찬,허정,Lee, Chang-Eun,Yun, Jong-Sup,Moon, Young-Chan,Hur, Jung 대한전자공학회 2004 電子工學會論文誌-TC (Telecommunications) Vol.41 No.5
위상 배열 안테나 기술을 이용하여 2차원적으로 빔의 방향을 조정할 수 있는 셀룰라 대역의 기지국 안테나를 개발하였다. 이 안테나는 복사 소자를 2×5로 배열하였으며, 14㏈i의 이득과 60°의 수평 빔폭, 그리고 13°의 수직 빔폭을 갖는다. 또한 수직면에서의 다운 틸팅 범위는 0°에서 12°이며, 수평면에서의 스티어링 범위는 -15° 에서 15°이다. 안테나를 필드에 설치한 후 DM 측정을 실시하여 빔 제어 성능을 확인하였다. Based on a Phased array technology, 2-dimensionally steerable base station antenna was developed at cellular band. The antenna, which consists of 2 by 5 radiating element, can provide 14㏈i gain with half power beam width of 0$^{\circ}$and 13$^{\circ}$ in horizontal and vertical plane respectively. It has beam scanning range of 0$^{\circ}$to 12$^{\circ}$in vertical down tilting and -15$^{\circ}$to 15$^{\circ}$in horizontal steering. The beam control Performance of antenna was verified by DM measurement on field trial.
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이창은,김기만,김우식,천승용,이상국,Lee, Chang-Eun,Kim, Ki-Man,Kim, Woo-Sik,Chun, Seung-Yong,Lee, Sang-Kook 한국음향학회 2014 韓國音響學會誌 Vol.33 No.4
This paper presents an underwater acoustic communication with nonlinear chirp modulation. The information is carried by the carrier amplitude, frequency or phase in the most common underwater acoustic communications. However, the proposed method includes the information within frequency variation of carrier wave for a symbol. Especially, as carrier wave the hyperbolic frequency modulated signal, one of the nonlinear chirp signal, is used and it is robust in the Doppler channel. The proposed method was analyzed and compared to conventional method by simulation. When the doppler shift existed, the error probability of the proposed method is reduced by 5~12 % than conventional method with linear frequency modulated signal. Sea trial was performed to analyze the performance of the proposed method. 본 논문에서는 수중음향통신에서 비선형 chirp 변조 신호를 사용하는 방법을 제안한다. 과거 대부분의 수중음향통신에서는 반송파의 크기, 주파수 또는 위상에 정보를 실었다. 이에 반해 제안된 방법은 심볼 내에서 반송파의 주파수 변화율에 정보를 포함하게 된다. 특히, 반송파로 비선형 chirp 신호의 일종인 하이퍼볼릭 주파수 변조 신호를 사용하는 경우, 도플러 채널에서 강한 특성을 가진다. 제안된 방법은 시뮬레이션을 통해 기존 방법과 비교 및 분석하였다. 제안된 방식과 선형 주파수 변조 신호를 갖는 기존 방식을 비교하였을 때 도플러 편이가 있는 경우 제안된 방법의 오차 확률이 평균 5~12 % 감소하는 것을 확인할 수 있었다. 또한 제안된 방법의 성능을 분석하기 위해 해상실험이 수행되었다.
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이창은(Chang-Eun Lee),조정권(Jung-Kwon Jo),김재덕(Jae-Deok Kim),이동근(Dong-Geun Lee),김원석(Won-Seok Kim),이상현(Sang-Hyeon Lee) 한국생명과학회 2017 생명과학회지 Vol.27 No.6
식중독의 원인 세균 및 병원성 세균 7종(Bacillus cereus (11.2, 20), Clostridium perfringens (2.4, 4.7), Escherichia coli (9.1, 9.1), Listeria monocytogenes (11.1, 20), Salmonella typhi (4.7, 9.1), Staphylococcus aureus (2.4, 4.7), Vibrio parahaemolyticus (2.4, 5.3))에 대해 한약재 추출물의 항균 활성을 측정하였다. 추출물은 증류수를 이용한 고온추출법(121℃에서 30분간)으로 제조하였고, 9가지 한약재 추출물의 수율은 0.5-33.4% 범위였다. 종이 디스크 확산법을 이용하여 박테리아에 대한 항균 활성을 평가하였을 때 황련, 지유, 오배자 추출물에서만 항균활성이 나타났다. 황련과 지유 추출물은 Clostridium perfringens과 Listeria monocytogenes에만 항균활성을 나타낸 반면, 오배자는 시험한 모든 세균에 대해 강한 항균 작용을 보였다. 오배자 추출물의 건조시료가 나타내는 최소억제농도(MIC)와 최소살균농도(MBC)는 2.4~20 mg/ml 범위였다. 본 연구결과는 추후 정제와 항균물질에 대한 연구를 통하여 기존 항균제의 보완제 및 식품의 유통기한 증진 등 항균소재로서 응용이 가능할 것으로 기대된다. The antibacterial activities of nine oriental herbal medicine extracts were measured against seven kinds of bacteria known to cause food poisoning and disease. The extracts were prepared with distilled water using an autoclave (121℃ for 30 min). The extraction yields of nine oriental herbal medicines were in the rage of 0.5%-33.4%. The antibacterial activities were evaluated using the paper-disc method. Extracts of Hwangryeon, Jiyu, and Ohbaeja showed antibacterial activities while the others did not. Extracts of Coptis japonica, Hwangryeon, and Sanguisorba officinalis, Jiyu showed antibacterial activities only against Clostridium perfringens and Listeria monocytogenes. Extracts of Rhus javanica, Ohbaeja showed the strongest antibacterial activities against all the bacteria tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of R. javanica extracts were in the range of 2.4~20 mg/ml for the bacteria tested. In conclusion, the extract of R. javanica showed superior antibacterial activities against the seven bacterial strains tested. The MIC and MBC values for each bacterium were represented as strain names (MIC, MBC), which were Bacillus cereus (11.2, 20), Clostridium perfringens (2.4, 4.7), Escherichia coli (9.1, 9.1), Listeria monocytogenes (11.1, 20), Salmonella typhi (4.7, 9.1), Staphylococcus aureus (2.4, 4.7), and Vibrio parahaemolyticus (2.4, 5.3). Further purification and study of this antibacterial material would be helpful for developing antibiotics or promoting synergistic effects with known antibiotics, producing antibacterial material for lengthening the shelf-life of food, and so on.
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