심전도 이상군과 대조군에 었어서 이완기 및 수축기 혈압과 총콜레스테롤과의 관계

김병원 ( Byung Weon Kim ),김영활 ( Young Hwal Kim ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3

We have examined the blood pressure and the value of total cholesterol of 2,438 person visited on Soonchunhyang university hospital in Kumi for the hea1th examination from Jauary to December in 1995. The patient that was shown abnormal ECG was 317 person (13%) of the hea1th examined person (2,438) and in the abnormal ECG patient, male were 199 person (19.2%) and female were 118 person (9.9%), and the emerging ratio ofabnormal ECG was shown 24.1 % in 60 ages, 19.6% in 50 ages and 9.9% in 40 ages eκ.Also we have analyzed the blood pressure and the value of total ch이esterol from the normal electrocardiogram group (317 person) , control group and the abnormal ECG group (317 person) which were selected randomly. πle resu1t was followed as below.1. In the normal ECG group, the diastolic and systolic blood pressure and the value of total cholesterol was 78.61 i= 16.51 mmHg and 119.38 i= 10.56 mmHg, and 187.23 i= 14.52 mgjdl respectively but in the abnormal ECG group, 83.89 i= 13.34 mmHg and 131.98 i= 21.09 mmHg, and 187.23 i= 14.52 mgjdl respectively. 2. In the abnormal ECG group, the average of the diastolic blood pressure was high (94.03 i= 12.20 mmHg) in the left venπicular hypertrophy (L VH) and total cholester,이 195.24 i= 35.41 mg/dl in the ischemia. In the normal and abnormal ECG group, over 100 mmHg in the diastolic blood pressure was 11 and 57 person, over 160 mmHg in the diastolic blood pressure was 4 and 40 person, and over 250 mg/dl in the total cholesterol was 6 and 20 person respectively. 3. In the correlation analysis, the correlation of the diastolic and systolic blood pressure, and the total cholesterol was r=0.10 ψ<0.05), r=O. lO ψ<0.05) and r=0.12 (p<0.05) respectively. From these results, we knew that the emerging ratio of abnormal ECG was high according to increasing ages (50-60 ages) , and the diastolic and systolic blool prelusure and the value of total cholesterol was increased correlatively in the abnormal ECG group than normal ECG group

구기자의 세포생장과 Betaine 생산에 미치는 질산염과 인산염의 영향

김병원(Byung-Weon Kim),최명석(Myung-Suk Choi),노광수(Kwang-Soo Roh) 한국생물공학회 1997 KSBB Journal Vol.12 No.2

구기자 현탁 세포배양으로부터 이단계 배양과 Elicitor에 의한 Betaine 생산

김병원(Byung-Weon Kim),노광수(Kwang-Soo Roh) 한국생물공학회 1998 KSBB Journal Vol.13 No.5

구기자 투여 간손상 흰쥐에서 GOT 및 GPT의 활성화 연구

김병원(Byung-Weon Kim),노광수(Kwang-Soo Roh) 대한의생명과학회 2000 Biomedical Science Letters Vol.6 No.3

구기자의 한방 약리효과를 보기 위하여 구기자 혼합 생체시료와 세포배양한 캘러스로부터 추출한 열수 추출물을 사염화탄소로 유발된 간장해 흰쥐 (SD계)에 하루 0.5 g-㎏을 경구 투여한 결과, 대조군 (GOT 949, GPT 640 karmen unit)에 비하여 혼합 생체시료 (GOT 760.4, GPT 540 karmen unit)와 세포배양 캘러스 (GOT 772.1, GPT 556.4 karmen unit) 처리구에서 거의 비슷한 혈청 중 GOT, GPT 활성의 상승을 억제하는 효과를 보였으며, 또한 비교약물 sylimarin 0.1 g/㎏ 투여군에서도 대조군에 비하여 혼합 생체시료 (GOT 492.6, GPT 320.4 kannen unit)와 세포배양 캘러스 (GOT 492.6, GPT 320.4 karmen unit) 처리구에서 거의 비슷한 혈청 중 GOT, GPT 활성의 상승을 억제하는 효과를 보였다. 이상의 결과를 통하여 볼 때 구기자 생체시료와 세포배양 캘러스는 간손상 흰쥐에 대한 간보호 효과가 동일하게 있는 것으로 판단된다. The present study was undertaken in order to investigate betaine production by tissue culture and its medicinal effect in Lycium chinense Mill. In order to investigate the protective effect of L. chinense on the hepatoxicity induced by CCl₄, 0.5 g/㎏ water extract of the compound mixture (leaves, roots and shoots) of L. chinense and its callus were fed to rat (SD line) once a day. As a result, the activity of GOT and GPT in the the group fed compound mixture (GOT 760.4 and GPT 540 Karmen unit) and callus (GOT 772.1 and GPT 556.4 Karmen unit) was decreased in the blood serume relative to the controlled rat group (GOT 949 and GPT 640 Karmen unit) and the same result was obtained in the group fed with 0.1 g/㎏ sylimarin (the activity of GOT and GPT was shown 492.6 and 320.4 Karmen unit respectively. These results strongly indicate that water extracts of the mixture and callus from L. chinense do have the same decreasing effect of GOT and GPT in the hepatotoxic rat induced by CCl₄.

표면피부온도 변화가 정중신경의 운동신경전도 속도에 미치는 영향

김병원(Kim Byung Weon) 한국보건기초의학회 2018 한국보건기초의학회지 Vol.11 No.2

In this study we evaluated the effect of a changing cutaneous temperature on the motor nerve conduction velocity (MNCV) of the median nerve. We measured the MNCV of the median mortar nerve of heathy subjects in their twenties. From our results, we know that the MNCV of the median nerve decreases as the cutaneous temperature decreases. This result is especially noticeable with females, as their MNCV decreased about 5.4% more than with males. Also it was shown that the latency and amplitude of median nerve were increased by a cold cutaneous temperature. Our results can be used as a reference point to check for abnormalities of a patient’s medial nerve.

An Investigation of Glyceollin I’s Inhibitory Effect on The Mammalian Adenylyl Cyclase

Dong-Chan Kim(김동찬),Nam Doo Kim(김남두),Sung In Kim(김성인),Chul-Soo Jang(장철수),Chang Oh Kweon(권창오),Byung Weon Kim(김병원),Jae-Ki Ryu(류재기),Hyun-kyung Kim(김현경),Suk Jun Lee(이석준),Seungho Lee(이승호),Dongjin Kim(김동진) 한국생명과학회 2013 생명과학회지 Vol.23 No.5

글리세올린 I 은 다양한 피부 질환의 예방과 치료에 유용한 물질로 주목을 받아왔다. 그러나 피부 멜라닌 형성에 결정적 역할을 담당하는 포유류의 아데니닐 고리화 효소(이하 mAC)에 대한 직접적인 결합 형태와 상호 작용에 대한 연구는 현재까지 연구 보고된 사례가 없었다. 글리세올린 I 의 mAC 활성 부위에 대한 결합 작용 기작을 규명하기 위하여 우선 글리세올린 I과 SQ22,536 (mAC에 결합하여 mAC의 활성을 억제하는 것으로 이미 잘 알려진 단일 화합 물질)의 mAC에 대한 결합 친화도와 결합 형태를 비교 분석 하였다. 글리세올린 I은 mAC의 활성부위에 존재하는 Asp 1018, Trp 1020, Asn 1025와 각각 3개의 수소결합을 형성하는 것으로 분석되었고 SQ22,536은 mAC 활성부위의 Asp 1018, Asn 1025와 2개의 수소결합을 형성하여 글리세올린 I이 상대적으로 우월하게 결합하는 것으로 분석되었다. 글리세올린 I은 또한 포스콜린(forskolin)에 의해서 유도되는 세포내 멜라닌 형성 2차 신호전달 물질인 cyclic AMP의 생성과 이로인해 유발되는 단백질 인산화 효소 A의 인산화를 효과적으로 억제하는 것을 멜라노마 세포 실험을 통하여 확인하였다. 또한 글리세올린 I 을 장시간 세포에 투여하여도 세포의 생존률에는 영향을 주지 않음을 확인하였다. 본 연구 통하여 규명된 글리세올린 I의 mAC 활성 억제 효능 및 멜라닌 생성 신호전달 기작을 조절하는 성질을 이용하여 향후 흑색종과 같은 다양한 피부 질환 치료제 개발 및 미백화장품 핵심 물질 개발에 활용될 수 있을 것으로 사료된다. Glyceollin I has gained attention as a useful therapy for various dermatological diseases. However, the binding property of glyceollin I to the mammalian adenylyl cyclase (hereafter mAC), a critical target enzyme for the down-regulation of skin melanogenesis, has not been fully explored. To clarify the action mechanism between glyceollin I and mAC, we first investigated the molecular docking property of glyceollin I to mAC and compared with that of SQ22,536, a well-known mAC inhibitor, to mAC. Glyceollin I showed superiority by forming three hydrogen bonds with Asp 1018, Trp 1020, and Asn 1025, which exist in the catalytic site of mAC. However, SQ22,536 formed only two hydrogen bonds with Asp 1018 and Asn 1025. Secondly, we confirmed that glyceollin I effectively inhibits the formation of forskolin-induced cAMP and the phosphorylation of PKA from a cell-based assay. Long term treatment with glyceollin I had little effect on the cell viability. The findings of the present study also suggest that glyceollin I may be extended to be used as an effective inhibitor of hyperpigmentation.

구기자나무 體細胞胚 人工種子 生産과 發芽

Jae Ki Kim(金宰琪),Byung Weon Kim(金柄源),Kwang Soo Roh(盧光洙) 한국육종학회 1996 한국육종학회지 Vol.28 No.3

An artificial seeds was developed using somatic embryos derived from the leaf callus of Lycium chinenes Mill. Somatic embryos were singly alginate-encapsulated to be used as artificial seeds. When cultured on MS basal medium, 95% of the non-encapsulated and 49% of the encapsulated somatic embryos were capable of germination. Among various concentrations of sodium alginate tested, the best result for bead formation was obtained in 3% sodium alginate. Of all tested media, 52% of somatic embryos were germinated on 2 MS medium but up to 60% on 3/2 MS medium. Characoal supplemented to the sodium alginated solution promoted shooting and rooting. The highest germination(80%) was obtained when artificial seeds was encapsulated with sodium alginated containing 0.5% charcoal. Artificial seeds did not lose the capacity for germination even after storing at 4℃ for 45 days. Shoots and roots of the encapsulated somatic embryos were induced after 4 weeks on 3/2 MS medium without growth regulators. Whole plantlets were successfully transplanted to potting soil for futher growth.

에어로빅스가 청각유발전위에 미치는 영향

박상남 ( Sang Nam Park ),김영활 ( Young Hwal Kim ),김병원 ( Byung Weon Kim ) 대한임상검사과학회 2006 대한임상검사과학회지(KJCLS) Vol.38 No.3

Up to now, there have been rare clinical studies on leaders and aerobics athletes. To get the useful data for protecting from auditory disorder, we selected 15 female aerobics leaders (experimental group) and 15 females (control group) unexperienced in aerobics and a without neurological and octolaryngological disorder. The average age was 34.87±8.80 (experimental group) and 34.07±8.45 (control group) years, and the average career of an aerobics leader (experimental group) was 8.33±4.73 years. We measured the auditory evoked potential (AEP) of the two groups treated with 70, 75 and 85 dB intensity from January 2006 to May 2006 and analyzed the absolute latency (AL) and interpeak latency (IPL) by the SPSS/pc+ 12.0 program. In the comparison of the AL between the experimental group and the control group according to intensity, both ears treated with 70 and 75 dB had a significant difference (p<0.05) in the Ⅰ, Ⅲ, Ⅴ wave and in theⅠ, Ⅴ wave respectively, and the experimental group treated with 85 dB showed a difference in the Ⅰ, Ⅲ, Ⅴ wave (left ear) and in theⅠ wave (right ear) respectively. The IPL of the two groups treated with various intensities had no prolongation. In the comparison of the AL between the experimental group and the control group according to ages, the experimental group in their 20s treated with 70 dB showed a significant difference (p<0.05) in the Ⅴ wave (left ear) and in the Ⅰ, Ⅲ, Ⅴ wave (right ear), and the experimental group in their 20s treated with 75 dB in theⅠ, Ⅲ wave (left ear) and in Ⅰ, Ⅲ, Ⅴ wave (right ear), and experimental group in their 20s treated with 85 dB in the Ⅴ wave (left ear) and in the Ⅲ, Ⅴ wave (right ear). The experimental group in their 30s treated with 70 dB had a significant difference (p<0.05) only in the Ⅴ wave (right ear). Only in the IPL of subjects in their 20s treated with 85 dB, Ⅲ-Ⅴ and Ⅰ-Ⅴ of both ears was extended. In the comparison of the AL and IPL according to career, there was no significant difference between the two groups. From this results, we concluded that the lower sound intensity (70 dB) showed a more significant difference in the experimental group than the control group. We concluded that the leader of aerobics exposed to louder sounds than normal people are affected by auditory neurological and octolaryngological disorders. So we think that the leaders of aerobics need to control the noise level for protecting themselves against a disease.

알츠하이머병 환자에서 콜린에스터라제 억제제의 투여에 의한 대뇌피질 국소혈류의 변화

문성식 ( Seung Sik Moon ),김영활 ( Young Hwal Kim ),김병원 ( Byung Weon Kim ) 대한임상검사과학회 2003 대한임상검사과학회지(KJCLS) Vol.35 No.1

Alzheimer`s Disease(AD) is a progressive, degenerative disease that attacks the brain resulting in impaired memory, thought processing, and behavior. This is associated with a loss in presynaptic cholinergic function. It has been suggested that cholinergic inhibitors could restore this function and improve some symptoms of AD. Previous studies have shown that cholinesterase inhibitors(ChEI) improve cognitive and global functions in patients with mild to moderate AD. This study aims to evaluate regional cerebral blood flow(rCBF) changes and contemporary clinical responses such as cognitive and psychiatric symptoms after ChEI treatment. The subjects were eight at risk AD patients(four males and four females, mean age 69.63 years) recruited from the department of Neurology at Yeungnam University Medical Center between August 2000 and April 2002. The clinical diagnosis of AD was based on DSM-Ⅳ and NINCDS-ADRDA criteria. Huchinski ischemic scores of all the patients were below 1. The mean treatment duration was 30.38 weeks, ranging between 24 to 44 weeks. Four patients received Rivastigmine(Exelon®) 12mg after titration, three patients received Donepezil(Aricept®) 10mg during the whole period, and one patient received Donepezil 10mg after the initial 5mg for three weeks. The base line and follow up Ethylene Cysteine Diethylester Single Photon Emission Computed Tomography(99mTC-ECD SPECT) studies were done within one week prior to ChEI treatment and within one week following the study. Regions of interest(ROIs) were drawn over the left and right frontal lobe, temporal lobe, parietal lobe, and cerebellum. Region to cerebellar ratio(RCR)(each count in the ROIs divided by mean cerebellar ROI count) were calculated as a estimation of rCBF, and base line RCRs were compared with those of the follow up. The results display an overall increase in global cerebral blood flow through K-MMSE(Korean-Mini Mental State Examination), CDR(Clinical Dementia Rating), CDR-SB(Clinical Dementia Rating-Sum of Box), GDS(Global Deterioration Scale), and NPI(Neuropsychiatric Inventory, Cummings). We found that the most significant increase in blood flow occurred in the bilateral parietal lobes(p<0.05). Reduction in the rCBF is more profound on the left hemisphere in the base line(p<0.05) where there is a significant increase of rCBF after ChEI treatment compared with to right hemisphere(p<0.05).

성별 및 연령에 따른 안면신경마비 출현 양상에 관한 연구

황경희 ( Kyung Hee Hwang ),김병원 ( Byung Weon Kim ),김영활 ( Young Hwal Kim ) 대한임상검사과학회 2008 대한임상검사과학회지(KJCLS) Vol.40 No.2

Facial palsy is a general nerve disease which is occurred in a lateral incomplete or complete facial palsy. But it makes man restrict a social activity and so it is very important that we know the prognosis factors of a patient for medical care. We want to investigate the position and the patterns of the facial palsy according to sex and age. In this study, we performed the excitability test of the facial nerve on 103 patients (male 45 and female 58) which diagnosed as the Bell’s palsy having an acute facial palsy, and the rate of the facial palsy was 42% (43 case) in right oculi and 58% (60 case) in left oculi. The facial palsy was appeared widely from young to elderly patients and especially, the rate of the facial palsy was high in age groups from 40 to 50.