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김세은,황방연,김영호,김영중,이경순,이정준,Kim, Se-Eun,Hwang, Bang-Yeon,Kim, Young-Ho,Kim, Young-Choong,Lee, Kyong-Soon,Lee, Jung-Joon 한국생약학회 1997 생약학회지 Vol.28 No.4
Methanol extracts from 450 plants were screened for muttidrug-resistance reversing activity using drug sensitive KB-3-1 and multidrug-resistant KB-Vl cells. Among them, the extracts of Cynanchum wilfordii, Torilis japonica, Celastrus orbiculatus, Melia toosendan and Teminialia chebula strongly potentiated vinblastine cytotoxicity in KB-Vl cells. But their cytotoxicities to both sensitive KB-3-1 and resistant KB-Vl cells were in the same order of magnitude. These results indicate that the above samples would contain the active principles which do not exert their ativity solely by cytotoxicity.
자근으로부터 acetylshikonin의 분리 및 함량분석
황석연,황방연,강삼식,김창민,박정일,배기환,손건호,이승호,장승엽,강신정,노재섭,이경순,Hwang, Suk-Yeon,Hwang, Bang-Yeon,Kang, Sam-Sik,Kim, Chang-Min,Park, Jeong-Hill,Bae, Ki-Hwan,Son, Kun-Ho,Lee, Seung-Ho,Chang, Seung-Yeup,Kang, Shin-Jung 한국생약학회 2000 생약학회지 Vol.31 No.3
Lithospermi Radix has been used in traditional medicine for antimicrobial, antiinflammatory, immunostimulating, antitumor and wound-healing activities. For the quality control of this drug, acetylshikonin was isolated from the hexane extract of Lithospermum erythrorhizon(Boraginaceae) and identified by the spectroscopic evidences. A quantitative analysis of acetylshikonin using HPLC method showed that the average contents were $0.084{\pm}0.026%$ in 31 samples collected throughout the various regions of Korea.
정선채,황방연,오갑진,강신정,김미정,최우회,이경순,노재섭,Chung, Sun-Chae,Hwang, Bang-Yeon,Oh, Gab-Jin,Kang, Shin-Jung,Kim, Mi-Jeong,Choi, Woo-Hoi,Lee, Kyong-Soon,Ro, Jai-Seup 한국생약학회 1999 생약학회지 Vol.30 No.3
Seven compounds were isolated from the n-BuOH extract of the dried stem barks of Rhus javanica L. On the basis of physico-chemical, spectroscopic evidences and comparison with authentic samples, the compounds $1{\sim}7$ were identified as gallic acid (1), methyl gallate (2), scopoletin (3), scopolin (4), $1,\;2,\;3,\;4,\;6-penta-O-galloyl-{\beta}-D-glucose$ (5), orcinol (6) and $orcinol-{\beta}-D-glucoside$ (7). Among these compounds, scopolin, $orcinol-{\beta}-D-glucoside$ were isolated from this plant for the first time.
수치에 따른 숙지황 중의 5-hydroxymethyl-2-furaldehyde 함량분석
황석연,황방연,최우희,정한진,허재두,이경순,노재섭,Hwang, Suk-Yeon,Hwang, Bang-Yeon,Choi, Woo-Hoi,Jung, Han-Jin,Huh, Jae-Doo,Lee, Kyong-Soon,Ro, Jai-Seup 한국생약학회 2001 생약학회지 Vol.32 No.2
In order to determine the content of 5-hydroxymethyl-2-furaldehyde (5-HMF) in the Rehmanniae Radix Preparata, 5-HMF was isolated from the EtOAc fraction of the Rehmanniae Radix Preparata samples and identified by spectroscopic and physicochemical evidences. Twenty one samples prepared on the basis of increasing number of stewing times with wine were analyzed by HPLC. From this analysis, it was revealed that the samples processed under one to nine times of stewing showed increasing pattern in 5-HMF contents. And the samples processed under 9 to 18 times of stewing showed 0.53-0.74% in 5-HMF contents. However, for those samples processed more than 19 times, 5-HMF content profile showed decreasing pattern.
시판 오수유로부터 evodiamine의 분리 및 함량분석
황석연,황방연,주혜경,박정일,손건호,이승호,장승엽,강신정,노재섭,이경순,Hwang, Suk-Yeon,Hwang, Bang-Yeon,Ju, Hei-Kyoung,Park, Jeong-Hill,Son, Kun-Ho,Lee, Seung-Ho,Chang, Seung-Yeup,Kang, Shin-Jung,Ro, Jai-Seup,Lee, Kyong-Soon 한국생약학회 2001 생약학회지 Vol.32 No.2
Evodiae Fructus has been used in traditional medicine for the treatment of gastro- intestinal disorders, headache and migraine, and as a cardiotonic and analgesic. For the quality control of this crude drug, evodiamine was isolated from the methylene chloride extract of Evodia officinalis (Rutaceae) and identified by the spectroscopic evidences. A quantitative analysis of evodiamine using HPLC method exhibited that the average contents of evodiamine were $0.84{\pm}0.60%$ in 53 samples collected throughout the various regions of Korea.
시판 지각으로부터 Hesperidin의 분리 및 함량분석
한두일,황방연,황석연,박정일,손건호,이승호,장승엽,강신정,노재섭,이경순,Han, Doo-Il,Hwang, Bang-Yeon,Hwang, Suk-Yeon,Park, Jeong-Hill,Son, Kun-Ho,Lee, Seung-Ho,Chang, Seung-Yeup,Kang, Shin-Jung,Ro, Jai-Seup,Lee, Kyong-Soon 한국생약학회 2001 생약학회지 Vol.32 No.2
Aurantii Fructus has been used in traditional medicine as a digestant, expectorant, and in the treatment of diarrhea, anal prolapse. For the quality control of this crude drug, hesperidin was isolated from the ethylacetate fraction of Citrus aurantium (Rutaceae) and identified by the spectroscopic evidences. A quantitative analysis of hesperidin using HPLC method exhibited that the average contents of hesperidin were $2.13{\pm}1.16%$ in 57 samples collected throughout the various regions of Korea.
결명자로부터 aurantio-obtusin의 분리 및 함량분석
주혜경,황방연,강신정,장승엽,원도희,노재섭,이경순,Ju, Hei-Kyoung,Hwang, Bang-Yeon,Kang, Shin-Jung,Chang, Seung-Yeup,Won, Do-Hee,Ro, Jai-Seup,Lee, Kyong-Soon 한국생약학회 2001 생약학회지 Vol.32 No.2
For the quality control of Cassiae Semen, anthraquinone compound, aurantio-obtusin, was isolated from the MeOH extract of Cassiae Semen (Leguminosae) and identified by the spectroscopic analysis. A quantitative analysis of aurantio-obtusin using HPLC method showed that the average contents of aurantio-obtusin were $0.03{\pm}0.01%$ in 50 samples collected throughout the various regions of Korea.
이성우,황방연,김세은,김환묵,김영호,이경순,이정준,노재섭,Lee, Sung-Woo,Hwang, Bang-Yeon,Kim, Se-Eun,Kim, Hwan-Mook,Kim, Young-Ho,Lee, Kyong-Soon,Lee, Jung-Joon,Ro, Jai-Seup 한국생약학회 1995 생약학회지 Vol.26 No.4
Preliminary screening test of modulators for multidrug resistance with 400 medicinal plants was carried out by using human multidrug resistance cell line, KB-V1. Among active medicinal plants, the unripe fruits of Evodia officinalis showed a potent modulating activity of MDR. From MeOH extract of this plant, we isolated two indole alkaloids, rutaecarpine (1) and evodiamine (2), by repeated silicagel column chromatography. Rutaecarpine increased the cytotoxicities of vinblastine and taxol against multidrug resistance cells, but evodiamine showed no modulating activity in spite of its potent cytotoxic activities.
주혜경,황방연,안병태,김미정,최우회,조봉진,노재섭,이경순,Ju, Hei-Kyoung,Hwang, Bang-Yeon,Ahn, Byong-Tae,Kim, Mi-Jeong,Choi, Woo-Hoi,Cho, Bong-Jn,Ro, Jai-Seup,Lee, Kyong-Soon 한국생약학회 2000 생약학회지 Vol.31 No.3
Five compounds were isolated from the fruits of Evodia daniellii $H_{EMSLEY}$. Their structures were identified as 2-oxotridecanyl acetate, bergapten, limonin, squalene, ${\beta}-sitosterol$ on the basis of spectral data. Among these compounds, 2-oxotridecanyl acetate and squalene were isolated in this plant for the first time.