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배합사료의 크기, 공급횟수 그리고 사육밀도가 조피볼락 (Sebastes schlegelii) 치어의 성장에 미치는 영향
오대한 ( Dae Han Oh ),송진우 ( Jin Woo Song ),김민기 ( Min Gi Kim ),이봉주 ( Bong Joo Lee ),김강웅 ( Kang Woong Kim ),한현섭 ( Hyon Sob Han ),이경준 ( Kyeong Jun Lee ) 한국수산과학회 2013 한국수산과학회지 Vol.46 No.4
We performed four sets of feeding trials to establish optimum feed size (Exp-I), stocking density (Exp-II) and feeding frequency (Exp-III and IV) for Korean rockfish. In Exp-I, three different particle sizes of a commercial diet (small, 2.0-2.1 mm; medium, 2.4-3.2 mm; and large, 4.0-5.3 mm) were fed to four replicate groups of fish (22.8±0.1g), each of which was fed to apparent satiation for six weeks. In Exp-II, fish (44.3±0.4 g) were reared at four stocking densities (1.5, 3.0, 4.5 and 6.0 kg/m3) and fed a commercial diet to apparent satiation for four weeks. In Exp-III, fish (14.8±0.1 g) were fed a commercial diet once, twice, thrice, 4× or 5× a day at a feeding ratio of 3.25% of body mass. Another group of fish was fed the same commercial diet 5× a day to apparent satiation; this treatment was designated FS (five times satiation/day). In Exp-IV, fish (31.3±0.1 g) were fed a commercial diet once, twice, thrice or 4× a day to apparent satiation. Another group of fish was fed to apparent satiation once every 48 hours. In Exp-I, fish fed the large particle (4.0-5.3 mm) diet had a significantly higher feed conversion ratio and lower protein efficiency ratio than fish fed the small particle diet. In Exp-II, groups of fish reared at densities of 4.5 and 6.0 kg/m3 had significantly higher feed intake and growth performance than fish reared at 1.5 and 3.0 kg/m3. In Exp-III, fish fed to apparent satiation had significantly higher growth performances than fish fed once or 4× a day. A significantly higher feed conversion ratio and a lower protein efficiency ratio were obtained in the FS group. In Exp-IV, growth performance and feed utilization efficiency were not significantly affected by experimental variation in feeding frequency. Fish fed to apparent satiation once every 48 hours had better feed utilization than those in other treatments and growth performances of those were comparable. Therefore, the optimum feed particle size, stocking density and feeding frequency for Korean rockfish under conditions we used were 2.0-3.2 mm, 4.5-6.0 kg/m3, and once a day or once every 48 hour, respectively.
적수온(21-24℃)에서 사육한 성장기(317 g) 넙치(Paralichthys olivaceus)의 배합사료 적정 공급률
오대한 ( Dae Han Oh ),김성삼 ( Sung Sam Kim ),김강웅 ( Kang Woong Kim ),김경덕 ( Kyoung Duck Kim ),이봉주 ( Bong Joo Lee ),한현섭 ( Hyon Sob Han ),김재원 ( Jae Won Kim ),( Okorie Eme Okorie ),배승철 ( Sung Chul Bai ),이경준 ( K 한국수산과학회 2014 한국수산과학회지 Vol.47 No.4
We investigated the effects of feeding rate on the growth performance, blood components, and histology of grow- ing olive flounder Paralichthys olivaceus. Optimum feeding rate (initial fish mean weight: 316.7±6.18 g) was de- termined under the optimum water temperature. Two replicated groups of fish were fed a commercial diet at rates of 0%, 0.4%, 0.6%, and 0.8% of body weight (BW) per day, and to satiation. Feeding trial was conducted using a flow-through system with 10 1.2-metric ton aquaria receiving filtered seawater at 21-24°C for 3 weeks. Weight gain (WG) and specific growth rate (SGR) were significantly higher in fish fed to satiation (1.0% BW/day) than in those in other treatments. These parameters were negative and significantly lower in the starved fish than in fish fed the experimental diet at all feeding rates. There were no significant differences in WG and SGR among fish fed at 0.4%, 0.6%, and 0.8% BW/day. Hematocrit and hemoglobin in fish fed to satiation were significantly lower than those in other treatments. Histological changes of fish fed at 0.6% BW/day indicated that this group was in the best condition; differences were not found in tissues of fish fed at 0%, 0.6% and 1.0% BW/day. Broken-line regression analysis of weight gain showed that the optimum feeding rate of olive flounder weighing 317 g was 0.99% BW per day at the optimum water temperature.
사료 내 단백질과 지방 수준이 참돔(Pagrus major) 치어의 성장, 사료효율 및 비특이적 면역력에 미치는 영향
김성삼 ( Sung Sam Kim ),오대한 ( Dae Han Oh ),최세민 ( Se Min Choi ),김강웅 ( Kang Woong Kim ),김경덕 ( Kyoung Duck Kim ),이봉주 ( Bong Joo Lee ),한현섭 ( Hyon Sob Han ),이경준 ( Kyeong Jun Lee ) 한국수산과학회 2015 한국수산과학회지 Vol.48 No.3
A 3×3 factorial study was conducted to investigate the effects of dietary protein and lipid levels on the growth, feed utilization and innate immunity of red seabream Pagrus major. Nine diets consisting of three protein levels (42%, 46% and 50% crude protein) and three lipid levels (10%, 14% and 18% crude lipid) were formulated. Triplicate groups of red seabream were fed the experimental diets to apparent satiation (5-6 times a day, from 08:00 to 18:00 h at 2-h intervals) for 10 weeks. At the end of the feeding trial, the weight gain and specific growth rate of fish fed P46L14 (46% protein and 14% lipid), P50L10 (50% protein and 10% lipid) and P50L14 (50% protein and 14% lipid) were significantly (P<0.05) higher than those of fish fed P42L18 (42% protein and 18% lipid). The feed conver-sion ratios (FCR) of the fish were affected by dietary lipid levels (P<0.039), but not dietary protein levels. The FCR tended to increase with increasing dietary lipid levels from 10% to 18% with the 46% and 50% protein levels. The weight gain, protein efficiency ratio, specific growth rate, feed intake and survival of fish were not affected by either dietary protein or lipid levels. Myeloperoxidase activity in the group fed P50L14 (50% protein and 14% lipid) was significantly higher than that in the group fed P42L10 (42% protein and 10% lipid) or P50L18 (50% protein and 18% lipid). However, the myeloperoxidase activity of fish was not affected by either dietary protein or lipid level. The fish fed P46L14 (46% protein and 14% lipid) and P46L18 (46% protein and 18% lipid) showed significantly higher superoxide dismutase activity than did the fish fed P46L10 (46% protein and 10% lipid), P50L10 (50% protein and 10% lipid) of P50L18 (50% protein and 18% lipid). In conclusion, the optimum protein and lipid levels for the growth and feed utilization of juvenile red seabream were 46% and 14%, respectively, and the optimum dietary protein to energy ratio was 27.4 g/MJ.
박은미,조현국,홍경은,남보혜,김영옥,김우진,이상준,한현섭,이재용,김종식,장인권,정재훈,최태진,공희정,Park, Eun-Mi,Cho, Hyun Kook,Hong, Gyeong-Eun,Nam, Bo-Hye,Kim, Young-Ok,Kim, Woo-Jin,Lee, Sang-Jun,Han, Hyon Sob,Lee, Jae Yong,Kim, Jong-Sheek,Ja 한국해양바이오학회 2007 한국해양바이오학회지 Vol.2 No.1
Penaeidins은 새우류에 존재하는 항미생물성펩티드의 한 종류로서 새우의 외부 병원체에 대한 방어기작을 구성하는 중요한 인자이다. 본 연구에서는 대하 혈구세포의 cDNA library로부터 분리된 EST 클론을 분리 동정하였다. 아미노산 염기서열 분석과 계통수 분석을 통하여 본 연구에서 분리한 EST 클론이 대하의 Penaeidin 3-2 유전자와 아미노산 수준에서 동일함을 밝혔다. 대하의 Penaeidin 3-2는 signal peptides로 예상되는 19개 아미노산 잔기를 포함하여 전체 71개의 아미노산 잔기로 이루어져 있고, C-말단에는 3개의 이중황화결합을 형성할 수 있는 6개의 cysteine가 존재하였다. 대하 Penaeidin 3-2 전사체는 혈구세포, 간췌장, 근육 조직에서 발현되었으며, 특히 혈구세포에서 주로 많이 발현되었다. 흰반점바이러스의 인위감염 실험에서 바이러스 감염 후 대하 Penaeidin 3-2의 발현이 증가하였다. 이상의 결과들로부터 대하 Penaeidin 3-2가 자체 방어 작용에서 중요한 역할을 할 것으로 예상된다. Penaeidins are members of a special family of antimicrobial peptides existing in several species of shrimp and play a crucial role in the immunological defense of shrimp. In this study, we isolated and characterized one EST clone (penaeidin) from cDNA library of fleshy prawn Fenneropenaeus chinensis hemocytes. Amino acids sequence comparison and phylogenetic analysis with other known penaeidins revealed that our clone was completely identical to F. chinensis Penaeidin 3-2 (Accession no. ABC33920), which composed of 71 amino acids with a putative signal peptide (1-19) and a cysteine-rich domain (C-terminal part). The expression and distribution of Penaeidin 3-2 transcripts in shrimp were detected in hemocytes, hepatopancreas, and muscles, and that Penaeidin 3-2 was constitutively expressed mainly in hemocytes. The artificial infection of white spot syndrome virus to F. chinensis resulted in Penaeidin 3-2 mRNA up-regulation in hemocytes, suggesting that the possible role of Penaeidin 3-2 in host defense system of F. chinensis.
넙치( Paralichthys olivaceus ) 치어 사료에서 산 가수분해 농축대두박 (Acid-concentrated soybean meal)의 어분대체효과 및 소화율 평가
김성삼 ( Sung Sam Kim ),오대한 ( Dae Han Oh ),조성준 ( Seong Jun Cho ),서상현 ( Sang Hyun Seo ),한현섭 ( Hyon Sob Han ),이경준 ( Kyeong Jun Lee ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.6
This study was conducted to investigate the effects of fishmeal replacement with acid-concentrated soybean meal (ACSBM) on growth performance, blood biochemistry, and ingredient digestibility in juvenile olive flounder Para lichthys olivaceus . Six experimental diets were formulated to replace fishmeal protein with ACSBM at 0%, 20%, 30%, 40%, 50%, and 60% (designated ACSBM0, ACSBM20, ACSBM30, ACSBM40, ACSBM50, and ACSBM60, respectively). Triplicate groups of fish (initial fish mean weight: 14.3±0.03 g) were fed the experimental diets to apparent satiation (twice daily at 08:00 and 18:00 h). After a 12-week feeding trial, a total of 180 healthy fish were randomly distributed into three Guelph system tanks at a density of 60 fish/tank (initial fish mean weight : 50.6±2.4 g) to test the apparent digestibility coefficients of the ingredients (ACSBM, fishmeal, and soybean meal). Although negative effects were observed with ACSBM40, ACSBM50 and ACSBM60 after 12 weeks of feeding, up to 20% of the fishmeal protein could be successfully replaced with ACSBM without significant growth depression. Hemoglobin and hematocrit values of fish fed the ACSBM50 and ACSBM60 diets were significantly lower than those of fish fed the ACSBM0 diet. Glucose values of fish fed the ACSBM60 diet were significantly higher than those of fish fed the ACSBM0 and ACSBM20 diets. Digestibility of protein in ACSBM and soybean meal was 85.9% and 82.5%, respectively. Results indicated that at least 20% of fishmeal protein can be replaced by ACSBM in diets of juvenile olive flounder without supplementation of limiting amino acids.
어린 해삼(Apostichopus japonicus)용 사료 개발을 위한 실험사료의 사육효능 평가
김경덕,배기민,김강웅,이봉주,허상우,장지원,한현섭,Kim, Kyoung-Duck,Bae, Ki-Min,Kim, Kang-Woong,Lee, Bong-Joo,Hur, Sang-Woo,Jang, Ji Won,Han, Hyon-Sob 한국수산과학회 2017 한국수산과학회지 Vol.50 No.4
Six experimental diets for juvenile sea cucumbers Apostichopus japonicus were evaluated and compared with current commercial diets. Sea cucumbers (50 per tank; initial mean weight $2.3{\pm}0.1g$) in three replicates of seven groups were fed one of six experimental diets (ED1, 20% wild-harvested Sargassum thunbergii powder; ED2, 20% commercial S. thunbergii powder; ED3, 20% Undaria pinnatifida and Ascophyllum nodosum powders; ED4, 40% U. pinnatifida and A. nodosum powders; ED5, 10% brewer's yeast; and ED6, 10% sea shell powder) or a commercial diet (CD) for 28 weeks. The survival rate in all groups was 85-94%, with no significant difference among the experimental groups. The mean body weight of the sea cucumbers was significantly different among the experimental groups 6 weeks after the feeding trial. The final mean weight of the sea cucumbers was highest in ED1, ED3, and ED4, followed by ED6, ED5, ED2, and CD, in decreasing order. The results of this study suggest that U. pinnatifida and A. nodosum powders could provide a substitute for the S. thunbergii powder commonly used in commercial sea cucumber diets. Thus, the EP4 diet formulation, which contains no S. thunbergii, could be used as a practical feed for juvenile sea cucumbers.
양식산 참조기 및 수조기 미성어의 계절별 성장 경향과 저수온 내성 특성
조기채 ( Kee Chae Cho ),강덕영 ( Duk Young Kang ),강희웅 ( Hee Woong Kang ),한현섭 ( Hyon Sob Han ) 한국어류학회 2012 韓國魚類學會誌 Vol.24 No.1
본 연구는 조기류(참조기, 수조기)의 양식생물학적 기초 자료를 확보하고자 육상수조에서의 성장 특성 및 저수온 노출에 대한 영향을 조사하였다. 1년 사육기간동안 수조기 가 참조기에 비해 성장이 빨랐고, CF는 모두 9월에 가장 높게 나타났으며, DFI는 모두 9~10월에 최대값을 보인 후 낮아졌다. 사료계수는 참조기가 2.20, 수조기가 1.34였다. 완 만한 수온 하강에 대한 영향은 참조기와 수조기 미성어 모 두 4.7˚C 이하에서 폐사가 발생하였으며, 조기류 월동의 임 계수온은 9˚C로 나타났다. In the present study, we investigated the characteristic of growth and the tolerance of cold temperature in small yellow croaker and yellow drum, the results shows that the growth of yellow drum were faster than those of small yellow croaker with high food convergency efficiency, In condition factor (CF), the results shows that the values of yellow croaker and yellow drum were significantly higher in September, respectively. In mortality according to cold shock, two fishes was died under 4.7˚C in slow temperature descending test, but was both died under 9˚C in acute temperature descending test.
김강웅 ( Kang Woong Kim ),최정욱 ( Jeong Wook Choi ),김경덕 ( Kyoung Duck Kim ),한현섭 ( Hyon Sob Han ),안철민 ( Cheul Min An ),이봉주 ( Bong Joo Lee ),최윤희 ( Youn Hee Choi ),남택정 ( Taek Jeong Nam ) 한국수산과학회 2012 한국수산과학회지 Vol.45 No.6
This study examined the effects of dietary glycoprotein extracted from Porphyra yezoensi on growth performance and resistance against the pathogenic bacteria Edwardsiella tarda in olive f lounder. A porphyra-originated glycoprotein (P) was extracted using sequential processes of water and ethanol treatment. P extracts were added to a f ishmeal-based diet at concentrations of 0.0, 0.5, and 1.0% (designated as Con, P0.5, and P1.0, respectively). Fish were fed one of the three experimental diets for 10 weeks. All f ish groups exhibited over 96.7% survival during the experimental period. Results indicated that the f ish fed diets containing P showed an increase in growth performance, including enhanced weight gain, specif ic growth rate, and feed eff iciency. An increase in insulin-like growth factor (IGF-1) was observed in the f ish fed the P1.0 diet, as compared to those fed Con. At the end of the 10-week feeding trial, all f ish were infected with E. tarda, and accumulated mortality was monitored for 8 days. Fish fed the Con diet exhibited increasing mortality from day 3 to the end of the challenge test, whereas the mortality of P-fed f ish ceased at day 5. We suggest that supplementation with P-originated glycoprotein in aquafeed may increase growth performance and resistance against pathogenic bacteria in olive f lounder juveniles.