강원도 10종 산채류의 항산화 및 인지능력개선 효능 평가

한웅호,최선일,김민종,이옥환,Han, Xionggao,Choi, Sun-Il,Kim, Min-Jong,Lee, Ok-Hwan 한국식품영양학회 2019 韓國食品營養學會誌 Vol.32 No.6

The purpose of this study was to investigate effect of methanolic extracts of 10 kinds of wild vegetables cultivated in Gangwon province on antioxidant activity, acetylcholinesterase, and β-secretase inhibitory activities. Results showed that among the wild vegetables, Aralia elata(Miq.) Seem shoot extract exhibited the highest total phenol content (84.65±1.08 mg GAE/g) and total flavonoids content (70.77±0.55 mg RE/g), respectively. The antioxidant activity of wild vegetables extracts was measured by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. Aralia elata(Miq.) Seem shoot extracts had the highest DPPH and ABTS scavenging activity (90.16%, 40.18% at 2 mg/mL). As a result, Aralia elata(Miq.) Seem shoot extract was the most effective in terms of acetylcholinesterase inhibitory activity (35.94% at 1 mg/mL). In the β-secretase activity assay, all 10 kinds wild vegetables extracts showed low inhibitory activity, and Aralia elata(Miq.) Seem shoot extract had highest inhibitory activity among the 10 wild vegetables extracts was 14.99%. Taken together, these results showed that Aralia elata(Miq.) Seem shoot extract has potential cognition improvement impact, suggesting that it may provide an effective strategy for improving cognition.

곰피추출물의 지표성분 Dieckol의 분석법 개선 및 검증

한웅호,최선일,문효,이세정,오건,진희구,오현지,김은진,김종욱,이부용,이옥환,Han, Xionggao,Choi, Sun-Il,Men, Xiao,Lee, Se-jeong,Oh, Geon,Jin, Heegu,Oh, Hyun-Ji,Kim, Eunjin,Kim, Jongwook,Lee, Boo-Yong,Lee, Ok-Hwan 한국식품위생안전성학회 2022 한국식품위생안전성학회지 Vol.37 No.3

This study was to investigate an analytical method for determining dieckol content in Ecklonia stolonifera extract. According to the guidelines of International Conference on Harmonization. Method validation was performed by measuring the specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ) of dieckol using high-performance liquid chromatography-photodiode array. The results showed that the correlation coefficient of calibration curve (R²) for dieckol was 0.9997. The LOD and LOQ for dieckol were 0.18 and 0.56 ㎍/mL, respectively. The intra- and inter-day precision values of dieckol were approximately 1.58-4.39% and 1.37-4.64%, respectively. Moreover, intra- and inter-day accuracies of dieckol were approximately 96.91-102.33% and 98.41-105.71%, respectively. Thus, we successfully validated the analytical method for estimating dieckol content in E. stolonifera extract.

표준화된 곰피추출물의 항산화 활성 및 콜레스테롤 개선 효과

한웅호,김우혁,최선일,문효,이세정,진희구,오현지,강다혜,김형빈,이부용,이옥환,Han, Xionggao,Kim, Woo-Hyeok,Choi, Sun-Il,Men, Xiao,Lee, Se-jeong,Jin, Heegu,Oh, Hyun-Ji,Kang, Dahye,Kim, HyungBin,Lee, Boo-Yong,Lee, Ok-Hwan 한국식품위생안전성학회 2021 한국식품위생안전성학회지 Vol.36 No.4

본 연구에서는 표준화된 곰피추출물의 항산화 및 콜레스테롤 개선에 대한 효능평가를 통해 건강기능식품 소재로서의 가치를 검토하기 위해 총 폴리페놀, 총 플라보노이드 및 dieckol 함량을 측정하였으며 DPPH, ABTS radical 소거능, reducing power 및 FRAP 활성을 통하여 곰피추출물의 in vitro 항산화 활성을 조사하였고 표준화된 곰피추출물의 HMG-CoA reductase 저해 활성 및 세포 내 콜레스테롤 생성 억제 효능을 평가하였다. 표준화된 곰피추출물의 총 폴리페놀, 총 플라보노이드 및 dieckol 함량은 각각 9.64±0.04 mg GAE/g, 2.72±0.08 mg RE/g, 27.42±0.66 mg/g으로 나타났다. 표준화된 곰피추출물의 in vitro 항산화활성, HMG-CoA reductase 저해활성 및 세포 내 콜레스테롤 생성 억제 효능은 농도의존적으로 증가하는 경향을 보였으며 이는 표준화된 곰피추출물에 함유되어 있는 페놀성 화합물에 기인된 효능으로 사료되며 항산화성분, 항산화 효과, 콜레스테를 개선 효능간의 상관관계가 있음을 확인하였다. 향후, 표준화된 곰피추출물에 대한 in vivo 모델에서의 전임상 연구 및 작용기전 입증되면 인체적용시험을 통해 이중기능성을 갖는 건강기능식품의 개발이 가능할 것으로 사료된다. Ecklonia stolonifera, which belongs to the family Laminariaceae, is an edible perennial brown marine alga that is widely distributed, and is rich in polyphenols, including dieckol. Here, we investigated the radical scavenging activities of E. stolonifera extract (ESE) using various in vitro models. We further evaluated the effect of ESE on the cholesterol secretion inhibition activity in HepG2 cells, as well as the hydroxymethylglutaryl-coenzyme A (HMG-CoA) reductase activity. Our results showed that the total phenol, total flavonoid, and dieckol contents of ESE were 9.64±0.04 mg GAE/g, 2.72±0.08 mg RE/g and 27.42±0.66 mg/g, respectively. The antioxidant activity of ESE increased in a dose-dependent manner. In addition, the ESE inhibited cholesterol secretion from HepG2 cells with anti-HMG-CoA reductase activity. These results suggested that ESE possesses antioxidant and anti-cholesterol activities, and can therefore be used as a preclinical bioresource for development of health functional foods.

동과 추출물(HR1901-W)의 지표성분 2-Furoic acid 분석법 개발 및 검증

최선일 ( Sun-il Choi ),한웅호 ( Xionggao Han ),문효 ( Xiao Men ),이세정 ( Se-jeong Lee ),박미혜 ( Mi-hye Park ),이옥환 ( Ok-hwan Lee ),양정모 ( Jung-mo Yang ),최예은 ( Ye-eun Choi ),조주현 ( Ju-hyun Cho ) 강원대학교 농업생명과학연구원 2021 강원 농업생명환경연구 Vol.33 No.3

As interest and demand for functional foods increase, standardization becomes important to manage the quality of raw materials and products. Analytical methods for the standardization of bioactive compounds must be created in tandem with the development of functional foods. Therefore, the aim of this study was to validate an analytical method for the determination of 2-furoic acid in Benincasa hispida extract (HR1901-W). The 2-furoic acid analysis method was validated by evaluating the linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ), using a high-performance liquid chromatography-photodiode array spectrum. Our results showed that the correlation coefficients of the calibration curve for 2-furoic acid was 0.9999. Furthermore, the LOD and LOQ for 2-furoic acid were 0.10 μg/mL and 0.32 μg/mL, respectively. The range of inter-day and intra-day precision values (RSDs, relative standard deviation) for 2-furoic acid were 0.13%-0.56% and 0.27%-0.33, respectively, and the inter-day and intra-day accuracy values (recovery) were 98.47%-102.74% and 98.77%- 102.72%, respectively. In addition, the content of 2-furoic acid in HR1901-W was found to be 412.39 ± 0.91 μg/dry weight in grams. These results indicate that the analytical method used in this study should be appropriate for the quantitative analysis of 2-furoic acid in HR1901-W and considered for use as basic data for the standardization of HR1901-W.

정선 갓의 영양성분 및 세포기반 항산화 활성 분석

권희연,최선일,조봉연,최승현,심완섭,한웅호,장길웅,최예은,여진희,조주현,이옥환,Kwon, Hee-Yeon,Choi, Sun-Il,Cho, Bong-Yeon,Choi, Seung-Hyun,Sim, Wan-Sup,Han, Xionggao,Jang, Gill-Woong,Choi, Ye-Eun,Yeo, Jin-Hui,Cho, Ju-Hyun,Lee, Ok-Hwan 한국식품영양학회 2019 韓國食品營養學會誌 Vol.32 No.5

The purpose of this study was to demonstrate the quality characteristics of Brassica juncea cultivated in Jeongseon (BJJ), South Korea. We analyzed the nutritional components and antioxidant activity of BJJ. As a result of the free sugar analysis, the contents of glucose and fructose in BJJ were $0.29{\pm}0.02g/100g$ and $0.10{\pm}0.00g/100g$, respectively. The major fatty acids were palmitic acid, octadecenoic acid and stearic acid. The palmitic acid was the highest at 31.22% of all fatty acids. The major minerals were identified as Ca, P, K, Mg and Na. The contents of vitamin $B_1$, vitamin $B_2$, vitamin $B_6$, vitamin C and vitamin E in BJJ were $0.02{\pm}0.00mg/100g$, $0.087{\pm}0.01mg/100g$, $0.02{\pm}0.00mg/100g$, $0.56{\pm}0.06mg/100g$ and $0.20{\pm}0.03mg\;{\alpha}-TE/100g$, respectively. As a result of the free amino acid analysis, total amino acid contents in BJJ were $2,801.21{\pm}115.38mg/100g$. L-proline content was the highest ($744.30{\pm}119.06mg/100g$) in BJJ. BJJ extract inhibits reactive oxygen species production in 3T3-L1 adipocytes. Also, BJJ extract exhibits a protective effect on oxidative stress in $H_2O_2$-induced human dermal fibroblast. These results indicate that BJJ comprises various valuable nutrients which can be used as functional food ingredients.

피부 섬유아세포에서 갈참나무 잎 추출물의 항산화 및 항노화 효능

최선일,이종석,이사라,여주홍,정태동,조봉연,최승현,심완섭,한웅호,이진하,김종대,이옥환,Choi, Sun-Il,Lee, Jong Seok,Lee, Sarah,Yeo, Joohong,Jung, Tae-Dong,Cho, Bong-Yeon,Choi, Seung-Hyun,Sim, Wan-Sup,Han, Xionggao,Lee, Jin-Ha,Kim, Jong Dai 한국식품위생안전성학회 2018 한국식품위생안전성학회지 Vol.33 No.2

본 연구는 예부터 약용으로 사용되었거나, 현재 식품공전에 식품원료로 사용이 가능한 것으로 등록된 국내 산림지역에 자생하는 식물을 식품산업에 활용하고자 선행연구에서 우수한 항산화 활성을 보인 갈참나무 잎을 본 연구에서 사용하였다. 70% 에탄올을 이용하여 추출한 갈참나무 잎을 이용하여, hydrogen peroxide로 산화적 스트레스를 유도한 피부 섬유아세포에서의 세포 보호효과, 세포내 항산화 효과 및 항노화 효과를 측정하였다. 세포 독성을 평가한 결과 25, 50 및 $100{\mu}g/mL$의 갈참나무 잎 추출물을 처리하였을 때 모두 독성을 나타내지 않았으며, hydrogen peroxide로 산화적 스트레스를 유도한 상태에서는 세포를 보호하여 농도 유의적으로 세포생존율이 증가하였다. 특히, $100{\mu}g/mL$의 농도에서는 양성대조군으로 사용한 $50{\mu}M$ ascorbic acid 수준까지 세포 생존율이 증가하였다. 세포내 항산화 효과를 확인 하기위해 사용한 $H_2-DCFDA$ assay에서는 형광현미경과 형광흡광도 측정에서 모두 농도 유의적으로 세포내 ROS 저감 활성을 확인하였고 갈참나무 잎 추출물을 $100{\mu}g/mL$ 농도로 처리했을 때는 $50{\mu}M$ ascorbic acid와 비슷한 세포내 항산화 효과를 나타내었다. 또한 SA-${\beta}$-galactosidase assay를 이용한 갈참나무 잎 추출물의 피부 섬유아세포에 대한 항노화활성은 ROS 생성 억제 효과와 유사한 경향으로 갈참나무 잎 추출물의 농도 유의적으로 세포 노화 억제효과를 확인하였다. 이상의 결과를 종합하여 볼 때 갈참나무 잎 추출물이 hydrogen peroxide로 인한 산화적 스트레스 상태에서 세포 보호효과, 항산화 효과 및 항노화 효과가 관찰되어 기능성 식품원료로서의 활용도가 매우 넓을 것으로 판단된다. The skin of the human body occupies the largest surface area of the body and acts as a protection for the person's internal organs. As such, the skin is a major target of oxidative stressors, and these oxidative stressors are known to contribute to skin aging over the course of time. For the most part, an antioxidant is an effective approach to utilize to prevent symptoms related to the reactive oxygen species (ROS)-induced aging of the skin. Therefore, we investigated the antioxidant and anti-aging activity of the leaves of the Quercusaliena Blume extract (QBE). In our study, we confirmed that the cell viability tested with XTT {2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide innersalt} assay was not affected up to a concentration of $100{\mu}g/mL$. In addition, the cell viability of HDF cells induced by hydrogen peroxide was recovered from 81% to 104% after treatment with QBE, which showed the greater protective effect than that of ascorbic acid. Treatments of QBE dose-dependently inhibited reactive oxygen species (ROS) production in HDF cells induced by hydrogen peroxide, which correlated with their protective effects on cell viability. Since QBE treatment exhibited the suppression effect of skin aging by decreasing the ROS production, QBE could be used as a not only natural anti-aging but also antioxidant resource.

좁은잎천선과 및 조릿대 혼합 추출물의 항산화 성분과 항산화 활성

권희연(Hee-Yeon Kwon),최선일(Sun-Il Choi),한웅호(Xionggao Han),문효(Xiao Men),장길웅(Gill-Woong Jang),최예은(Ye-Eun Choi),강준철(Jun-Chul Kang),조주현(Ju-Hyun Cho),이옥환(Ok-Hwan Lee) 한국식품과학회 2020 한국식품과학회지 Vol.52 No.4

본 연구에서는 HR02/04(8:2)-W에 대한 항산화 성분 및 생리활성을 평가하기 위하여, p-coumaric acid 함량, 총 플라보노이드 및 총 페놀 함량, DPPH, ABTS 라디칼 소거능, FRAP 활성, reducing power, ORAC value를 측정하였다. 또한, 피부 섬유아세포에서 hydrogen peroxide에 의하여 산화적 스트레스가 유도된 세포모델에서의 HR02/04(8:2)-W의 산화 손상 보호효과를 측정하기 위하여 XTT assay 및 H₂-DCFDA assay를 진행하였다. HPLC를 이용한 p-coumaric acid 함량 분석 결과, HR02/04(8:2)-W 내의 pcoumaric acid는 75.62±1.56 mg/100 g 함유되어 있는 것으로 나타났으며, 총 플라보노이드 함량 및 총 페놀 함량은 각각 21.57±0.84 mg RE/g, 21.25±1.31 mg GAE/g으로 측정되었다. DPPH 및 ABTS 라디칼 소거활성 시험에서 HR02/04(8:2)-W의 농도 유의적으로 소거활성이 증가하는 것을 확인하였으며, FRAP 활성 및 reducing power 측정에서도 우수한 항산화 활성을 보였다. ORAC assay 결과 control 보다 5배 이상 높은 수치를 보이며 효과적으로 라디칼 저해 활성을 보였다. 항산화 실험에서의 뛰어난 항산화 활성은 HR02/04(8:2)-W에 함유된 페놀류들에 의한 것으로 판단된다. 피부 섬유아세포를 이용한 실험에서, HR02/04(8:2)-W을 300 μg/mL의 농도까지 세포의 생존력에 영향을 미치지 않는 것으로 나타났으며, hydrogen peroxide 처리에 의하여 56%까지 감소되었던 세포 생존율을 최대 78%까지 증가시키며 효과적으로 세포를 보호했음을 확인하였다. H₂-DCFDA 염색을 통하여 세포내의 ROS의 양을 측정하였을 때, hydrogen peroxide처리에 의하여 control군 대비 137%까지 증가하였던 ROS를 최대 89% 까지 감소시킴으로써 세포 내 활성산소를 효과적으로 억제하였다. 이상의 결과를 종합하여 볼 때, HR02/04(8:2)-W이 뛰어난 항산화 생리활성 기능을 가지고, 세포 내 활성산소를 효과적으로 제거할 수 있는 소재임이 확인되었으며, 천연물 유래 기능성 식품원료로서의 활용 가능성이 매우 넓을 것으로 판단된다. The purpose of this study was to evaluate the antioxidant components and activities of HR02/04(8:2)-W, a mixture of S. quelpaertensis Nakai and F. erecta var. sieboldii. We investigated the p-coumaric acid, total flavonoid, and total phenol contents. To evaluate the antioxidant efficacy, we measured the 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity, FRAP activity, reducing power, and ORAC value. We observed the protective effect of hydrogen peroxide against cell damage in human dermal fibroblasts. As a result of the experiment, the p-coumaric acid, total flavonoid, and total phenol contents were 75.62±1.56 mg/100 g, 21.57±0.84 mg rutin equivalent (RE)/g, and 21.25±1.31 mg gallic acid equivalent (GAE)/g, respectively. In the experiments on antioxidant activity, HR02/04(8:2)-W was found to have significantly increased antioxidant activity. In the human dermal fibroblasts, the HR02/04(8:2)-W treated groups could effectively protect cells against oxidative damage. In this study, we confirmed that HR02/04(8:2)-W is a material with effective physiological antioxidant activity.

아마란스 안토시아닌의 안정성과 항산화 작용 조사

이진하(Jin-Ha Lee),이혜빈(Hye-Bin Lee),최선일(Sun-Il Choi),정태동(Tae-Dong Jung),조봉연(Bong-Yeon Cho),최승현(Seung-Hyun Choi),심완섭(Wan-Sup Sim),한웅호(Xionggao Han),이옥환(Ok-Hwan Lee) 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.12

본 연구에서는 아마란스 안토시아닌 색소의 온도별(4, 25 및 40°C), pH별(3.0, 7.0, 및 9.0), 당에 대한 안정성을 12일간 4일 간격으로 색소 함량, 총 페놀 함량, DPPH 라디칼 소거능, 아질산염 소거능 및 FRAP를 측정하여 평가하였다. 아마란스 안토시아닌 색소는 저온 4°C과 pH 3.0에서 색소함량의 변화가 적었으며, glucose, xylose를 첨가 시 당의 첨가에 따른 색소 함량의 큰 변화는 보이지 않았으나 다른 당에 비해 fructose 첨가에 의해 색소 함량의 감소가 다소 크게 측정되었다. 아마란스의 항산화 효과를 측정한 결과 아마란스 추출물 1, 2.5, 5 mg/mL의 DPPH 라디칼 소거능은 37.67±0.87%, 57.65±0.52%, 88.09±0.10%로 평가되었고, 아질산염 소거능은 아마란스 추출물의 농도를 2.5, 5 및 10 mg/mL로 처리하였을 때 각각 61.53±0.03%, 64.54±0.46% 및 75.70±0.32%로 평가되었다. 또한, FRAP 활성은 아마란스 추출물 2.5, 5 및 10 mg/mL 농도에서 0.34±0.00, 0.40±0.01, 0.53±0.01로 추출물의 농도가 증가함에 따라 항산화 활성도 증가하였다. 이상의 결과는 향후 아마란스를 활용한 가공식품 미 천연 식품 색소 개발을 위한 기초자료로 활용할 수 있을 것으로 생각된다. This study investigated the stability of anthocyanin and antioxidant activities from amaranth baby leaf extracts. Amaranth baby leaf was examined to investigate the effects of pH, temperature, and sugars on the stability of anthocyanin. Antioxidant compounds and activities were measured based on total phenolic content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities, nitrite scavenging activity, and ferric ion reducing antioxidant power (FRAP). In the results, the stability of anthocyanin rapidly decreased at 40°C, while the stability of anthocyanin slowly decreased at 4°C. The type of sugar (glucose, fructose, xylose) did not influence the stability of anthocyanin. The stability of anthocyanin was reduced in the following order: pH 3> pH 9> pH 7.0. Total phenolic content was 5.62±0.12 mg gallic acid equivalent/g, DPPH radical scavenging activity of amaranth baby leaf extracts ranged from 37.67±0.87 to 88.09±0.10%, and FRAP value increased depending upon the concentrations of amaranth baby leaf extracts and ascorbic acid. The nitrite scavenging ability of amaranth baby leaf extracts ranged from 61.53±0.03 to 75.70±0.32%. In conclusion, amaranth baby leaf can be used as a potential source of natural antioxidant compounds and natural food colorants.

청경채 및 경수채 어린잎 추출물의 항산화활성

권희연(Hee-Yeon Kwon),이수미(Su-Mi Lee),최선일(Sun-Il Choi),조봉연(Bong-Yeon Cho),최승현(Seung-Hyun Choi),심완섭(Wan-Sup Sim),한웅호(Han Xionggao),장길웅(Gill-Woong Jang),이옥환(Ok-Hwan Lee),박덕환(Duck-Hwan Park) 한국식품저장유통학회 2019 한국식품저장유통학회지 Vol.26 No.3

표준화된 고려엉겅퀴 추출물의 아질산염 소거능 및 항염증효과

권희연(Hee-Yeon Kwon),최선일(Sun-Il Choi),조봉연(Bong-Yeon Cho),최승현(Seung-Hyun Choi),심완섭(Wan-Sup Sim),한웅호(Han Xionggao),장길웅(Gill-Woong Jang),이옥환(Ok-Hwan Lee) 한국식품저장유통학회 2019 한국식품저장유통학회지 Vol.26 No.3