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      • KCI등재

        연구보문 : 환경미생물 ; 미생물혼합제제 처리가 토양의 미생물상과 화학적 특성 및 시설 채소 생육에 미치는 영향

        류일환 ( Il Hwan Ryu ),정수지 ( Su Ji Jeong ),한성수 ( Seong Soo Han ) 한국환경농학회 2012 한국환경농학회지 Vol.31 No.4

        BACKGROUND: The urgency of feeding the world`s growing population while combating soil pollution, salinization and desertification requires suitable biotechnology not only to improve crop productivity but also to improve soil health through interactions of soil nutrient and soil microorganism. Interest in the utilization of microbial fertilizer has increased. A principle of nature farming is to produce abundant and healthy crops without using chemical fertilizer and pesticides, and without interrupting the natural ecosystem. Beneficial microorganisms may provide supplemental nutrients in the soil, promote crop growth, and enhance plant resistance against pathogenic microorganisms. We mixed beneficial microorganisms such as Bacillus sp. Han-5 with anti-fungal activities, Trichoderma harziaum, Trichoderma longibrachiatum with organic material degrading activity, Actinomycetes bovis with antibiotic production and Pseudomonas sp. with nitrogen fixation. This study was carried out to investigate the mixtures on the soil microflora and soil chemical properties and the effect on the growth of lettuce and cucumber under greenhouse conditions. METHODS AND RESULTS: The microbial mixtures were used with each of organic fertilizer, swine manure and organic+swine manure and compared in regard to changes in soil chemical properties, soil microflora properties and crop growth. At 50 days after the treatment of microorganism mixtures, the pH improved from 5.8 to 6.3, and the EC, NO3-Na and K decreased by 52.4%, 60.5% and 29.3%, respectively. The available P2O5 and SiO2 increased by 25.9% and 21.2%, respectively. Otherwise, the population density of fluorescent Pseudomonas sp. was accelerated and the growth of vegetables increased. Moreover, the population density of E. coli and Fusarium sp., decreased remarkably. The ratio of bacteria to fungi (B/F) and the ratio of Actinomycetes bovis to fungi (A/F) increased 2.3 (from 272.2 to 624.4) and 1.7 times (from 38.3 to 64), respectively. Furthermore, the growth and yield of cucumber and lettuce significantly increased by the treatment of microorganism mixtures. CONCLUSION(S): These results suggest that the treatment of microorganism mixtures improved the chemical properties and the microflora of soil and the crop growth. Therefore, it is concluded that the microorganism mixtures could be good alternative soil amendments to restore soil nutrients and soil microflora.

      • SCOPUSKCI등재

        Curcumin애 의해 유도된 egr-1 및 c-myc 유전자에 의한 발현 감소가 CpG oligodeoxynucleotide로 촉진된 미성숙 B 림프종 BKS-2의 성장 증가 억제

        한성수(Seong-Su Han),정승태(Seung-Tae Chung) 한국독성학회 1999 Toxicological Research Vol.15 No.3

        To investigate the role of egr-1 and c-myc in immature murine B cell lymphoma BKS-2, we treated BKS-2 with curcumin and(or) CpG oligodeoxynucleotide(ODN). CpG ODN increased proliferation of BKS-2 cells up to 170% at 10 μM. The mRNA levels of egr-1 and c-myc were dramatically upregulated as early as 30 min following stimulation and decreased eventually with the 1 μM of CpG ODN. c-myc mRNA level was increased and peaked at 3 h. Curcumin treatment caused growth arrest of BKS-2 by dose-dependent manner and dramatically downregulated the expression of egr-1 and c-myc after 0.5 h until 6 h. Moreover, BKS-2 cells were growth arrested from CpG ODN mediated proliferation by the treatment with curcumin. 50 μM of curcumin treatment suppressed the expression of egr-1 and c-myc upregulated by CpG ODN. In summary. Curcumin caused the growth arrest of BKS-2 immature B-cell lymphoma and also inhibited the proliferation effect of CpG ODN to BKS-2 via downregulation of egr-1 and c-myc.

      • SCOPUSKCI등재

        pTiA6 의 virE Promoter 내 역반복서열의 Mutational Analysis

        한성수(Seong Su Han),심웅섭(Woong Seop Sim) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.3

        To investigate how the dyad symmetry region (DSR) and the distance between vir box and -35 sequence of the virE promoter plays a role in virE gene expression, two mutants were constructed by base substitution and insertional mutagenesis. The base substitutional mutation, a AA → CG substitution at positions -39 and -40 on the DSR, showed the level of β-galactosidase activity approximately 91% of the wild type virE promoter activity. Therefore, the native structure or the DSR seems to be not essential for virE expression. The insertional mutation, constructed by inserting 8 bp ClaI linker between -49 and -50, displayed the β-galactosidase activity at 12% of the native virE promoter activity. However, this striking reduction appears to be not caused by destruction of the native DSR structure, but by shifting the vir box far from putative -35 sequence.

      • SCOPUSKCI등재

        Sodium arsenite가 GM-CSF로 자극한 골수세포에 미치는 영향

        한성수(Seong Su Han),정혜주(Hye Joo Chung),박재현(Jae Hyun Park),조대현(Dae Hyun Cho) 한국독성학회 1999 Toxicological Research Vol.15 No.3

        The effects of arsenic on the proliferation and differentiation of bone marrow cells were studied by treatment of bone marrow cells with sodium arsenite and/or granulocyte/macrophage-colony stimulating factor (GM-CSF). GM-CSF stimulated the bone marrow cells, and the maximum growth rate of bone marrow cells following exposure to GM-CSF for 1, 3, 5 and 7 days was 133, 275, 400 and 208% of the control respectively. When bone marrow cells were treated with sodium arsenite ranging from 0.01 to 50 μM for 1, 3, 5 or 7 days, the growth of bone marrow cells was inhibited by dose- and time-dependent manner at the concentration higher than 1 μM, and the mean IC50 value was 6.2, 7.5, 9.0 and 7.7 μM, respectively. Apoptosis was also observed in all groups treated with 10 μM sodium arsenite regardless of duration of treatment. By exposure of bone marrow cells to sodium arsenite concomitant with 10 μM GM-CSF for 1, 3, 5 and 7 days, IC50 value in each group was shifted to the higher value. These results indicate that fast proliferating bone marrow cells by stimulation with GM-CSF are likely to be less vulnerable to inhibiting effects of arsenic on the cell growth, and apoptosis induced by high concentration of sodium arsenite could be suggested as one of the mechanism for arsenic in treatment of leukemia.

      • KCI등재

        연구보문 : 토양환경 ; 갈탄과 아미노산액 혼합제 시용이 벼, 배추와 고추의 생육 및 토양의 화학적 특성에 미치는 영향

        한성수 ( Seong Soo Han ),유기용 ( Ki Yong Yoo ),박민수 ( Min Su Park ),이영일 ( Young Il Lee ),백승화 ( Seung Hwa Baek ) 한국환경농학회 2010 한국환경농학회지 Vol.29 No.2

        갈탄과 아미노산액 혼합처리가 벼, 배추 및 고추의 지상부와 지하부 생육 및 수량에 미치는 영향을 조사한 후 생육증진효과를 검토하여 친환경농산물 생산용자재로의 활용가능성을 구명하고, 갈탄과 아미노산액에 화학비료를 감량하여 혼합처리하여도 작물재배 가능성이 있는지를 검토하며, 갈탄과 아미노산액 혼합처리 전후의 토양에 미치는 화학적 특성을 조사하여 토양개량제로써의 활용가능성을 구명하고자 하였다. 그 결과, 갈탄과 아미노산액 혼합처리한 구의 벼 생육은 화학비료 표준시비량 단용구보다 양호하였으며, 특히 갈탄과 아미노산액 150 kg/ha 처리구와 갈탄과 아미노산액 150 kg/ha 에 화학비료 표준시비량의 혼합처리구에서 다른 처리구보다 전반적으로 양호한 것으로 나타났다. 배추와 고추의 생육은 갈탄과 아미노산액 600 kg/ha 처리구와 갈탄과 아미노산액 600 kg/ha에 화학비료 표준시비량의 혼합처리구에서 다른 처리구보다 전반적으로 양호한 것으로 나타났다. 벼와 배추의 수량은 갈탄과 아미노산액 150kg/ha 처리구와 갈탄과 아미노산액 150 kg/ha 에 화학비료 표준시비량의 혼합처리구에서 양호하였고, 고추의 경우 갈탄과 아미노산액 600 kg/ha 처리구와 갈탄과 아미노산액 600kg/ha에 화학비료 표준시비량의 혼합처리구에서 양호하였다. 논과 밭토양에 갈탄과 아미노산액과 화학비료감량 혼합처리구의 유기물함량은 전반적으로 높았고, EC는 낮았다. pH와 유효인산의 경우 밭토양에서는 약간 높아지는 경향이었고, 논토양에서는 큰 변화가 없었다. 논과 밭토양에 갈탄과 아미노산액과 화학비료감량처리시 토양의 부분적인 화학적 특성, 특히 유기물함량을 높일 수 있었다. 그러나 이와 같은 결과들이 연용처리하여 재배하였을 경우에도 유사한 결과를 초래할지에 대한 연구는 추후 면밀히 검토되어져야 할 것으로 판단된다. For the study of possibility of practical use as an organic farm materials of the mixtures with lignite and amino acid solution, this experiment was carried out to investigate the effects of the mixtures on the growth and the yield of rice plant, chinese cabbage, and red pepper, and the effects of the mixtures on chemical properties of soil. Also, when the mixtures of the lignite plus amino acid solution and the chemical fertilizer were applied to these three crop cultivation area, authors want to know how can the loss in quantity of chemical fertilizer affects the growth and the yield of these crops. As the results, growth of rice plant applied with the mixtures of lignite and amino acid solution was better than that applied with the recommended rate of chemical fertilizer. Especially, the growth of rice plant appeared to be good at the treatment of 150 kg/ha of the mixed lignite with amino acid solution and at that of its mixtures and standard fertilization. Growth of chinese cabbage and red pepper was good at the application of 600 kg/ha of the mixed lignite with amino acid solution and at that of its mixtures and standard fertilization. Yield of rice and chinese cabbage was good at the treatment of 150 kg/ha of the mixed lignite with amino acid solution and at that of its mixtures and standard fertilization, and yield of red pepper was good at the application of 600 kg/ha of the mixed lignite with amino acid solution and at that of its mixtures and standard fertilization. The organic matter content increased and while the exchangeable cation decreased when the lignite mixed with amino acid solution and the loss in quantity of chemical fertilizer applied at paddy field. Incase of these treatments, pH and available phosphorus increase at upland field, but did not change at paddy field.

      • KCI우수등재

        초고분자량 PE젤 필름과 매트의 Zone 연신 거동

        김상용,한성수,최경식,Kim, Sang-Yong,Han, Seong-Su,Choe, Gyeong-Sik 한국섬유공학회 1989 한국섬유공학회지 Vol.26 No.7

        Gel films and mats of ultra-high molecular weight polyethylene were stretched by zone drawing to orient biaxially, and their physical and mechanical properties were studied. The maximum storage modulus of the drawn films at -15$0^{\circ}C$ and room temperature were 215 and 165 GPa, respectively. The drawability of gel film is better than that of gel mat but the maximum storage modulus of gel mat is higher than that of gel film.

      • KCI등재

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