감마선 조사된 남양진주의 검지

최현민,이보현,김영출,Choi, Hyun-Min,Lee, Bo-Hyun,Kim, Young-Chool 한국결정성장학회 2012 韓國結晶成長學會誌 Vol.22 No.1

남양진주에 $Co^{60}$을 이용하여 0.1~100 kGy까지 선량의 범위에서 방사선 조사 실험을 행하였다. 더불어 방사선 조사된 남양진주의 검지를 위해 전자상자성공명(EPR)과 아미노산분석(AAA)을 행하였다. EPR 분석에서 방사선 조사후, 방사선 조사전에는 없었던 free radical이 생성되었으며 $CO_2^-$ radical의 g-factor는 $2.001{\pm}0.002$이었다. 진주층의 아미노산 분석 결과 방사선 조사후의 glutamic acid는 11.43 %, alanine은 3.11 %, histidine은 43.75 %의 감소량을 보였으며, 이는 일부 아미노산이 파괴되었음을 보여준다. 본 연구의 결과로 EPR 분석은 방사선 조사된 남양진주의 검지에 적합하였다. We have been performed on $Co^{60}$ ${\gamma}$-ray irradiation of South Sea cultured pearls, with the absorbed dose of 0.1~100 kGy at room temperature. In addition, it was investigated electron paramagnetic resonance (EPR) and amino acid analysis (AAA) for detection of Gamma irradiated South Sea cultured pearls. It was observed that the irradiated South Sea cultured pearls revealed additional free radical peak such as $CO_2^-$, at a g-factor of $2.001{\pm}0.002$ in EPR spectra. From the amino acid analysis (AAA), it was shown that some of amino acid in the protein of the nacre destroyed after ${\gamma}$-ray; glutamic acid residue by 11.43 %, alanine by 3.11 %, and histidine by 43.75 %. It was useful to detect the irradiated South Sea cultured pearls by EPR measurement in our study.

담배 조직배양에서 6 - Phosphogluconater Dehydrogenase 의 정제와 성질에 관한 연구

최현민,김승수 ( Hyun Min Choi,Soung Soo Kim ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.4

One 6-phosphogluconate dehydrogenase isoenzyme, designated 6PG DH II, was purified from tobacco callus culture line (Nicotiana tobacum L., var. Virginia 115) utilizing ammonium sulfate precipitation, DEAE-cellulose chromatography, Red Sepharose CL-6 B affinity chromatography followed by Sephadex G-100 gel filtration. The purified enzyme was homogeneous according to the criteria of polyacrylamide gel electrophoresis. 6 PG DH II appears to have a dimeric structure with subunit molecular weight of 44,000 and native molecular weight of approximately 83,000. Double reciprocal plot of reaction velocity vs. 6-phosphogluconate concentrations indicated Km values of 17 μM with a marked inhibition of initial velocity at high 6-phosphogluconate concentrations.The NADP^+ double reciprocal plot showed an abrupt increase in the apparent Km and Vmax with increasing NADP^+. The two Km`s for low and high NADP^+ concentrations were 6.5 μM and 70 μM, respectively. The pH optimum of the enzyme was 8.5 in Tris-HCl and 8.0 in phosphate buffer. MgCl₂ was not required for the enzymatic activity. Furthermore, MgCl₂ inhibited the activity at high concentration.

침탄 두께에 따른 피로강도 영향 특성 평가

최현민,박용하,신용택,김명현,Choi, Hyun Min,Park, Yong Ha,Shin, Yong Taek,Kim, Myung Hyun 대한용접접합학회 2014 대한용접·접합학회지 Vol.32 No.4

Carburizing treatments are the important way to developing fatigue strength and wear resistance. It is well known that the case depth is one of the most significant parameters determining fatigue strength. In this study, 3-point bending fatigue test was conducted to evaluate fatigue strength for the carburized depth with 18CrNiMo7-6 steel. As a result, fatigue strength increased with effective case depth decreased. It is shown that hardness in case hardened layer played principal role in the fatigue strength.

감마선 조사된 남양진주의 검지 II

최현민,이보현,김영출,Choi, Hyun-Min,Lee, Bo-Hyun,Kim, Young-Chool 한국결정성장학회 2012 韓國結晶成長學會誌 Vol.22 No.4

방사선 조사된 남양진주는 광투과 검사, 확대 검사, 형광반응 검사 등의 보석학적 검사방법으로는 감별이 어렵지만, ESR 분석을 통해 조사된 남양진주와 조사되지 않은 남양진주의 감별이 가능하였다. 본 연구에서는 조사선량이 증가함에 따른 $CO^-_2$ radical의 변화와 관련된 정보를 제공할 목적으로 진주층과 핵을 분리하여 분석하였으며, 핵보다는 진주층에서 $CO^-_2$ radical이 더 명료하게 나타남을 발견하였다. It is very difficult to detect irradiated South Sea cultured pearls (SSCPs) at a low dose by gemological methods (e.g. optical transmission method, microscopic examination, UV fluorescence reaction, and so on). In this study, however, it was possible to distinguish between irradiated SSCPs from un-irradiated SSCPs by ESR analysis. We have analyzed the pearl nacre and nucleus by separating them for the purposed of providing information about the change of $CO^-_2$ radical as growing absorbed doses. And it was found out that the signal of $CO^-_2$ radical of the nacre is more distinct than the nucleus.

단기간의 금연과 일회성 유산소 운동이 흡연자의 심혈관 반응에 미치는 영향

송준경(Jun Kyung·choi),최현민(Hyun Min Choi),김종경(Jong Kyung Kim),노호성(Ho Sung Nho) 한국사회체육학회 2012 한국사회체육학회지 Vol.0 No.48

The purpose of this study was to investigate cardiovascular responses to a bout of aerobic exercise and acute smoking cessation in smoker. nine collegiate male smoker who have been smoking(duration of smoking: 7.5 ± 0.9yrs; number of cigarettes per day: 15.5 ± 1.5) participated in this study. All subjects performed PEI to stimulate Group IV metaboreceptor at immediately after smoking, 24hours after smoking cessation and smoking after a bout of aerobic exercise. they were measured SBP, DBP, HR, SV, CO, TVC using by finometer(FMS co, Netherland). As a result, compared to immediately after smoking, SBP, DBP, and MAP were significantly decreased 24-hours after smoking cessation at rest and PEI(p<0.05). In addition, smoking after a bout of aerobic exercise caused by post-exercise hypotension indicated that SBP was significantly decreased compared to immediately after smoking(p<0.05). also, the changes in DBP and MAP at rest and handgrip exercise were significantly smaller compared to immediately after smoking(p<0.05). Thus, the results showed that smoking cessation during 24-hours and a bout of aerobic exercise relieve excess blood pressure response and enhanced arterial baroreflex in collegiate male smoker. It is concluded that caused exercise pressor reflex by stimulated Group IV may be effective to improve excess blood pressure responses.

유압식 저항운동이 허약한 고령자들의 기능적 체력과 삶의 질에 미치는 효과

이성철(Sung-Chul Lee),최현민(Hyun-Min Choi),타케시마 노부오(Nobuo Takeshima) 한국생활환경학회 2014 한국생활환경학회지 Vol.21 No.3

This study was to evaluate the effectiveness of hydraulic resistance exercise (HDRE) for frail elderly. Forty five participants were divided into exercise group (79±7.6 yr, 11 men, 14 women), and control group (76±7.9 yr, 11 men, 9 women). An exercise group was trained by 6 types of exercise: Knee extension (KE), knee flexion (KF), chest press (CPr), chest pull (CPu), low back flexion (LF), and low back extension (LE) 2 days/week. HDRE was consisted of 12-week supervised program, 50 min/day, and one set with an appropriate loading for 15 repetitions to fatigue. As a results, muscle strength and power were significantly increased between 16.6~27.1% and 18.9~81.2% in the KE, KF, CPr, CPu, and LF (p < .05). The functional fitness were also increased about 19~43% except for sit & reach test, and health related quality of life were improved about 15~24% in the sf-36v2 test (p<.05). However, there were no significant changes in the control group, and the HDRE is an effective form of resistance training that provides benefits using low and moderate intensity of training for frail elderly.

설문지를 이용한 신체활동량과 참여강도가 중년여성의 체력 및 관상동맥 위험인자에 미치는 영향

안영환(Young Hwan An),박세정(Saejong Park),최현민(Hyun Min Choi),김종경(Jong Kyung Kim),노호성(Hosung Nho) 한국발육발달학회 2015 한국발육발달학회지 Vol.23 No.1

The purpose of the study was to investigate the effects of the volume of physical activity on obesity, physical fitness and coronary risk factors among the middle-aged women. physical activity was assessed from International Physical Activity Questionnaire (IPAQ) and were categorized by health enhancing physical activity group (PG), minimum activity group (MG), and non-activity group (NG) according to the volume of physical activity. Apparently healthy middle-aged women (n=163; 52.1±8.6 yrs) participated and they were classified as PG (n=46; 5,878±3547 MET·min/week), MG (n=64; 1,678±886 MET·min/week), and NG (n=52; 456±389 MET·min/week). Also, method of the study was categorized by vigorous activity and moderate activity according to the study of WHO (2010) and Mikael Fogeholm (2006). Measurement variables were body composition, physical fitness (hand-grip, sit-up, pacer, sit & reach, shuttle run, and long jump), total cholesterol (TC), low-density lipoprotein cholesterol (LDLC), high-density lipoprotein cholesterol (HDLC), and triglycerides (TG). One-way ANOVA was used with Tukey post-hoc (p<.05). In the result, PG had significantly low percent body fat comparing with MG or NG (p=.02). And, among physical fitness variables flexibility was higher in PG than in MG (p=.03). While HDLC (p=.007) was higher in the PG than MG or NG, LDLC (p=.024) was lower in the PG than MG. TG (p=.021) was lower in the PG than MG or NG. Vigorous activity group had significantly high pacer in classification as physical activity intensity. Glucose was lower in vigorous activity group than in moderate activity group (p=.02). In conclusion, it was generally low body fat rate and coronary risk factors for middle-aged women to increased volume of physical activity of daily life and participating vigorous activity indicated that was able to keep a high fitness standard.

진로진학프로그램에서 진로멘토의 참여가 고등학생의 진로준비행동과 그릿에 미치는 영향

최현민(Choi Hyun Min) 한국진로교육학회 2018 진로교육연구 Vol.31 No.3

본 연구는 고등학교 방과후 수업으로 진행되는 진로진학프로그램에서 진로멘토의 참여여부가 일반계 고등학생의 진로준비행동과 그릿에 미치는 영향을 확인하고자 한다. 연구대상은 S리더십센터와 연계하여 진로진학프로그램을 실시하고 있는 2개의 일반계 고등학교 2학년 학생을 대상으로 하였다. 실험집단은 진로에 도움을 줄 수 있는 대학생 진로멘토가 프로그램 속에 참여하어 조별 과정 진행을 지원하고, 개별 전공에 대한 정보 및 상담을 제공받은 참여자 75명을 대상으로 선정하였다. 통제집단은 실험집단과 동일한 프로그램을 진행하였으나, 진로멘토의 참여 없이 강의 및 워크샵에 참여한 학습자 45명을 대상으로 선정하였다. 연구도구는 진로준비행동(박완성, 2003)검사와 그릿(Duckworth, 2007)검사를 사용하였으며, 자료분석으로 t-검정을 실시하였다. 설문수거 후, 프로그램 탈락자, 불성실한 답변과 무응답 설문 6부를 제외한 총 114부의 자료가 분석에 사용되었다. 연구결과는 다음과 같다. 첫째, 진로멘토가 참여한 진로진학프로그램을 실시한 실험집단이 진로멘토의 참여없이 진행한 통제집단 보다 진로준비행동 평균이 17.2점 향상되었으며, 통계적으로 유의미한 차이가 확인되었다. 둘째, 진로멘토가 참여한 진로진학프로그램을 실시한 실험집단이 진로멘토의 참여없이 진행한 통제집단 보다 그릿 평균이 11.2점 향상되었으며, 통계적으로 유의미한 차이가 확인되었다. 하위영역별로 살펴보면, 진로준비행동 중 정보수집활동, 직업체험활동, 그릿에서 관심의 일관성 영역의 통계적 차이가 확인되었다. 본 연구의 결과를 통해, 진로진학프로그램 운영에서 진로멘토의 관계성과 영향력을 확인할 수 있었다. 특히 학생들의 실질적인 진로준비행동 및 그릿 향상을 위해서 진로멘토가 적극적으로 활용되어야 하며, 이를 반영한 체계적이고 구체적인 진로진학프로그램 실행이 현장에서 이루어져야 할 필요가 있다는 것을 시사하고 있다. This study was to provide the data for the effects the career mentor in the career development program had on the high school students’ GRIT & career preparation behavior. To test these research purpose, A second-year high school student supported by career mentor in the career development program were selected as test group, and A second-year high school student without career mentor in the career development program were selected as control group. The career development program & Career preparation behavior test & GRIT test was used for research tools. The data were analyzed using t-test was performed. The summary of the principal results obtained from the study is as follows. First, the test group, for which the career development program with career mentor was performed, showed that the average career preparation behavior was increased by 17.2 points than the control group, and represented to have statistically significant difference. Second, the average GRIT of the test group, for which the career development program with career mentor was performed, was increased by 11.2 points than the control group, and represented to have statistically significant difference. This result was able to identify the relationship and influence of career mentor. And, it suggests that the appropriate career mentor need to be provided to students for upgrading student’s career preparation behavior & GRIT.

고활성수소수 복용이 대학 농구선수들의 근피로도에 미치는 영향

최현민(Hyun-Min Choi),이준희(Joon-Hee Lee),전종목(Jong-Mok Chun),노호성(Hosung Nho),김종경(Jong-Kyung Kim),전박근(Bak-Geun Jeon),박시영(Siyoung Park) 한국생활환경학회 2009 한국생활환경학회지 Vol.16 No.5

병원급 이상 의료기관의 간호등급 상승 요인 분석

최현민 ( Hyun Min Choi ),한남경 ( Nam Kyung Han ),이상규 ( Sang Kyu Lee ),김한성 ( Han Sung Kim ),최성경 ( Sung Kyoung Choi ),정우진 ( Woo Jin Chung ) 한국보건행정학회 2015 보건행정학회지 Vol.25 No.1

Background: The purpose of this study was to analyze the increase in Grade of Nursing Management Fee of medical institutions and establish a reasonable government policy by examining which factors affect the increase of nurse staffing. Methods: Analyzing data collected from the Health Insurance Review & Assessment Service resource management department with targets of 1,104 medical institutions. The study period was 5 years from June 30, 2008 to June 30, 2013. SAS ver. 9.2 (SAS Institute Inc., Cary, NC, USA) was used for statistical analysis. The data was analyzed by a chi-square test and also conducted muiltivariate logistic regression analyses for variables of basic characteristics, human resource characteristics, and material resources. Results: Adjusted odds ratio (AOR) of the rise in Grade of Nursing Management Fee among other hospitals compared to hospitals owned by government or universities was 0.264. The AOR in hospitals established after November 2006 compared to those before June 1995 was 2.383. The AOR in Gangwon, Chungcheng South, and Jeolla South Provinces compared to Seoul was 0.084, 0.036, and 0.194, respectively. The AOR in hospitals with more than 6.75 specialists per 100 beds compared to those with less than 6.75 specialists per 100 beds was 7.514. The AOR in hospitals with more than 17.48 nurse per 100 beds compared to those with less than 17.48 nurse per 100 beds was 3.300. The AOR in hospitals with 50% to 75% bed utilization, 75% to 90% bed utilization and more than 90% bed utilization compared to those with less than 50% bed utilization was 5.428, 9.884, and 10.699, respectively. The AOR in hospitals with one magnetic resonance imaging (MRI) and more than two MRI compared to those with no MRI was 2.018 and 2.942, respectively. Conclusion: This result has showed policies to induce the rise in Grade of Nursing Management Fee among old hospitals and the incentive system for local medical institutions are needed. Also we need to develop a governmental policy for medium-small hospitals with low operation rate of beds and insufficient medical personnel and number of equipment in hospitals.