생후 7일, 16일된 circling mouse 청각 뇌줄기에서 N-메틸-D 아스파르트산염 수용체(NMDA receptor)에 대한 면역염색학적 분포

최인영,박기섭,김혜진,디라즈 마스키,김명주,Choi, In-Young,Park, Ki-Sup,Kim, Hye-Jin,Maskey, Dhiraj,Kim, Myeung-Ju 한국현미경학회 2010 Applied microscopy Vol.40 No.2

글루타메이트 수용체는 신경연접의 발달에서 신경연접과 정제 등에 중요한 역할을 한다. 포유류 청각뇌줄기의 가쪽위올리브 핵-안쪽마름섬유체핵 신경연접에서의 흥분성 신경전달물질의 전달은 글루타메이트와 같은 많은 흥분성 신경전달물질의 전달을 조절하므로 발생학적 연구에 매우 유용한 모델이다. 귀먹음은 흥분성 회로 또는 억제성 회로의 신경연접에서 신경전달물질들의 변화뿐 아니라 형태학적 변화도 초래하게 된다. 이런 이유로 선천적으로 태어나면서 달팽이 기관이 퇴화되는 선회생쥐는 귀먹음의 병태생리를 연구하는데 가장 좋은 동물모델이다. 그러나, 선회생쥐에서 이런 NMDA 수용체 각각의 아형들이 발달에 따라 어떻게 나타나는지에 관해서는 거의 알려진 사실이 없다. 따라서, 본 연구에서는 선천적으로 속귀에 돌연변이를 가진 선회생쥐를 이용하여 면역조직화학염색법으로 생후 7일과 16일의 쥐를 정상쥐와 비교해서 글루타메이트성의 흥분성 신호가 전달되는 청각뇌줄기의 가쪽위올리브핵(LSO)에서 N-메틸-D-아스파르 트산염(NMDA) 수용체의 아형인 NR1, NR2A, NR2B의 분포를 각각 조사하였다. 생후 7일째 선회생쥐의 NR1에 대한 면역반응성의 세기는 정상군에서 $128.67\pm8.87$로 나타났고 이형접합체에서는 $111.06\pm8.04$, 동형접합체에서는 $108.09\pm5.94$으로 나타났다. 그리고 생후 16일째 선회생쥐에서는 정상군에서 $43.83\pm10.49$, 이형접합체에서는 $40\pm13.88$, 동형접합체에서는 $55.96\pm17.35$으로 나타났다. 생후 7일째 선회생쥐의 NR2A에 대한 면역반응성의 세기는 정상군에서 $97.97\pm9.71$, 이형접합체에서 $102.87\pm9.30$, 동형접합체에서 $106.85\pm5.79$로 나타났다. 생후 16일 선회생쥐의 가쪽위올리브핵에서의 NR2A에 대한 면역반응성 세기는 정상군에서 $47.4\pm20.6$, 이형접합체에서 $43.9\pm17.5$, 동형접합체에서 $49.2\pm20.1$로 나타났다. 생후 7일의 선회생쥐의 NR2B의 면역반응성 세기는 정상군에서 $109.04\pm6.77$, 이형접합체에서 $106.43\pm10.24$, 동형접합체에서 $105.98\pm4.10$으로 나타났다. 생후 16일째 선회생쥐의 NR2B의 면역반응성의 세기는 정상군에서 $101.47\pm11.50$, 이형접합체에서 $91.47\pm14.81$, 동형접합체에서 $93.93\pm15.71$으로 나타났다. 이 결과들은 생후 7일과 16일의 선회생쥐의 가쪽위올리브핵에서 NMDA 수용체의 면역반응성의 변화를 나타낸 것으로 인간 귀먹음의 주요한 병태생리를 파악하는 데 기본적인 자료를 제시할 수 있을 것이다. Glutamate receptors may play a critical role in the refinement of developing synapses. The lateral superior olivary nucleus (LSO)-medial nucleus of trapezoid body (MNTB) synaptic transmission in the mammalian auditory brain stem mediate many excitatory transmitters such as glutamate, which is a useful model to study excitatory synaptic development. Hearing deficits are often accompanied by changes in the synaptic organization such as excitatory or inhibitory circuits as well as anatomical changes. Owing to this, circling mouse whose cochlea degenerates spontaneously after birth, is an excellent animal model to study deafness pathophysiology. However, little is known about the development regulation of the subunits composing these receptors in circling mouse. Thus, we used immunohistochemical method to compare the N-Methyl-D-aspartate receptor (NMDA receptor) NR1, NR2A, NR2B distribution in the LSO which project glutamergic excitatory input into the auditory brainstem, in circling mouse of postnatal (p) 7 and 16, which have spontaneous mutation in the inner ear, with wild-type mouse. The relative NMDAR1 immunoreactive density of the LSO in circling mouse p7 was $128.67\pm8.87$ in wild-type, $111.06\pm8.04$ in heterozygote, and $108.09\pm5.94$ in homozygote. The density of p16 circling mouse was $43.83\pm10.49$ in wild-type, $40\pm13.88$ in heterozygote, and $55.96\pm17.35$ in homozygote. The relative NMDAR2A immunoreactive density of LSO in circling mouse p7 was $97.97\pm9.71$ in wild-type, $102.87\pm9.30$ in heterozygote, and $106.85\pm5.79$ in homozygote. The density of LSO in p16 circling was $47.4\pm20.6$ in wild-type, $43.9\pm17.5$ in heterozygote, and $49.2\pm20.1$ in homozygote. The relative NMDAR2B immunoreactive density of LSO in circling mouse p7 was $109.04\pm6.77$ in wild-type, $106.43\pm10.24$ in heterozygote, and $105.98\pm4.10$ in homozygote. the density of LSO in p16 circling mouse was $101.47\pm11.5$ in wild-type, $91.47\pm14.81$ in heterozygote, and $93.93\pm15.71$ in homozygote. These results reveal alteration of NMDAR immunoreactivity in LSO of p7 and p16 circling mouse. The results of the present study are likely to be relevant to understand the central change underlying human hereditary deafness.

중풍(中風)의 요일별(曜日別) 발생(發生)과 위험요인(危險要因)과의 상관성(相關性)에 관(關)한 연구(硏究)

김용형,최인영,마미진,강아미,최동준,한창호,이원철,전찬용,조기호,최선미,Kim, Yong-Hyung,Choi, In-Young,Ma, Mi-Jin,Gang, A-My,Choi, Dong-Jun,Han, Chang-Ho,Lee, Won-Chul,Jun, Chan-Yong,Cho, Ki-Ho,Choi, Sun-Mi 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.1

Objective : This study was aimed to investigated the correlation between the stroke incidence by day of the week and risk factors in acute stroke patients. Methods : From October in 2005 to October in 2007, 673 acute stroke patients wereincluded. Patients were hospitalized within 14 days after the onset of stroke in DongGuk University International Hospital, Kyungwon University In-cheon Oriental Medical Hospital or the Department of Cardiovascular and Neurologic Diseases (Stroke Center), Kyung Hee University Oriental hospital. We investigated general characteristics, stroke types, age group, educational backgrounds, medical history, lifestyle (the impact of stress, exercise, smoking and drinking) and Sasang constitution according to the stroke incidence by day of the week. Results : After examining each participants with day of the week, the order of days by incidence was Monday, Wednesday, Sunday, Saturday, Tuesday, Thursday and Friday respectively. Monday and Tuesday showed the highest ratio. As the aspect of demographic data of subjects, the group under 55 years and from 55 years to 70 years showed the highest ratio on Monday. In the group between 25 years to 55 years, the ratio, by the classification of actual productive age, was statistically higheron Monday. In addition, the ratio showed statistically higherby educational background, from elementary school to high school and over high school. In regard tothe medical aspect, incidence of cerebral hemorrhage showed higher ratio on Sunday, and incidence of brain infarction was higheron Monday. However, there was no difference of the past history between the two groups. With regard tothe aspect of lifestyle of subjects, the group with huge stress before the incidence of stroke showed a higher ratio of stroke incidence on Monday. Participants who didn't exercise regularly and participants who didn't smoke or drink showed higher ratio of stroke incidence on Monday. However, this was not important statistically. In regard to the aspect of Sasang constitution, Soeumin showed the highest ratio of stroke incidence on Wednesday, Taeuminon Monday, and Soyangin on Saturday and Monday. Conclusion : According to these results, several cardiovascular risk factors affect stroke incidences on Monday. Further studies will be needed to help understand the correlation between stroke incidence by day of the week and risk factors in acute stroke patients.

자가면역성 만성 췌장염으로 진단된 소아 1예

최인영,진소희,최경단,김경모,Choi, In-Young,Jin, So-Hee,Choi, Kyung-Dan,Kim, Kyung-Mo 대한소아소화기영양학회 2007 Pediatric gastroenterology, hepatology & nutrition Vol.10 No.2

저자들은 자가면역 질환이 없는 건강한 소아가 황달을 동반하지 않는 급성 복통으로 내원하여 혈청 amylase, lipase의 지속적인 상승과 혈청 IgG 증가, 자가항체(ANA, ANCA) 양성, 방사선 영상에서 췌장 실질종대와 췌장 미부 주췌관의 불규칙적 협착으로 자가면역성 만성 췌장염으로 진단받고 스테로이드와 azathioprine을 경구 복용하였으며 미부 주췌관 협착의 풍선확장술을 시행하고 회복된 1예를 경험하였기에 보고하는 바이다. We present a case of autoimmune chronic pancreatitis in a previously healthy child without any history of autoimmune disease. A 12-year-old boy was admitted to the hospital with abdominal pain. The serum amylase, lipase, and IgG levels were elevated and autoantibodies (antinuclear antibody, antineutrophil antibody) were detected. An abdominal CT (computed tomographic) scan revealed diffuse enlargement of the pancreas. ERCP (endoscopic retrograde cholangiopancreaticography) demonstrated an irregular stricture of the main pancreatic duct in the pancreas tail. After two years of oral steroid and immunosuppressive drug therapy, the clinical, laboratory and radiological findings were improved. The patient has been symptom-free for 18 months after the discontinuation of medication.

고혈압 환자에서 혈압 조절 여부에 따른 기혈수(氣血水) 변증(辨證)과 삶의 질 비교

최인영,한창호,최동준,정승현,신길조,Choi, In-Young,Han, Chang-Ho,Choi, Dong-Jun,Jung, Seung-Hyun,Shin, Gil-Jo 대한한방내과학회 2010 大韓韓方內科學會誌 Vol.31 No.4

This study is about a comparison of controlled and uncontrolled hypertension groups regarding comprehensive diagnosis of Qi blood water and quality of life. We surveyed "controlled and uncontrolled hypertension patients" using questionnaires for comprehensive diagnosis of Qi blood water, SF-36 and HTN QoL (Measurement Scale for the quality of life in hypertensive patients). There was no difference in comprehensive diagnosis of Qi blood water between the controlled and uncontrolled hypertension groups. Within the controlled hypertension group, the patients diagnosed with a Qi deficiency, Qi stagnation, Qi counterflow, blood deficiency, and water retention received lower total scores in SF-36 and HTN QoL than in undiagnosed patients. Within the uncontrolled hypertension group, the patients diagnosed with Qi deficiency, blood deficiency, and water retention got lower total scores in SF-36 and HTN QoL than in undiagnosed patients. These results were statistically significant. These results are insufficient that we and use comprehensive diagnosis of Qi blood water for a diagnosis tool of hypertension. But if we have better studies that make up for weak points, these results will help to make a diagnosis tool for hypertension.

급성기 중풍환자의 사상체질과 중풍 경고증상, 전조증상과의 상관성 연구

최인영,김용형,최동준,한창호,이원철,전찬용,조기호,최선미,Choi, In-Young,Kim, Yong-Hyung,Choi, Dong-Jun,Han, Chang-Ho,Lee, Won-Chul,Jun, Chan-Yong,Cho, Ki-Ho,Choi, Sun-Mi 대한한방내과학회 2007 大韓韓方內科學會誌 Vol.28 No.4

Objective : This study investigated the relationship between warning signs and Sasang constitution (SC) in acute stroke patients. Methods : From October in 2005 to March in 2007, 629 acute stroke patients were studied. Patients were hospitalized within 14 days after the onset ofstroke at one of 3 oriental medical hospitals in Seoul and Kyong-gi province. We assessed the type of SC of acute stroke patients by Questionnaire for Sasang Constitution Classification II (QSCC II). We investigated warning signs (motor weakness, blindness, dysarthria, sensory disturbance, finger dullness, facial & eyelid spasm, neck stiffness), general characteristics, etc. Results : This study showed a higher proportion of Tae-eum & So-eum experience upper extremity sensory disturbance but So-yangexperience lower extremity sensory disturbance. Smoker So-eumexperience 1st & 2nd finger dullness more than non-smoker So-eum. So-eum women experience motor weakness more than So-eum men. Conclusion : Besides these results, we could observe almost no relationship between warning signs and Sasang constitution (SC) in acute stroke patients. More data from prospective cohort studies will help people better understand the relationship between warning signs and Sasang constitution (SC) in acute stroke patients.

공통과학 교과서 화학영역의 STS 내용 분석

최인영,김윤희,이석희,문성배,Choi, In Young,Kim, Yun Hi,Lee, Seok Hee,Moon, Seong Bae 대한화학회 2001 대한화학회지 Vol.45 No.3

본 연구는 제 6차 교육과정에 의해 편찬된 12종의 검인정 공통과학 교과서 중 화학단원에서 전체 지면중 STS 내용의 포함정도를 알아보고 STS 내용을 단원별, Piel의 주제영역별, 활동영역별로 비교분석하였다. 그리고 교과서에 STS와 관련된 내용이 어느 정도 반영?품?있고, 교과서가 교육과정 목표에 어느 정도 부합되는지를 알아보았다. 12종의 공통과학 교과서 화학 영역에서는 STS 교육내용이 교과서 지면의 11.8%에서 33.6%로 평균 24.2%를 차지하고 있었다. STS 내용의 단원별 분포를 보면 현대과학과 가술 단원에 45.5%, 환경단원 38.7%, 에너지단원 29.1%, 물질단원14.0%로 현대과학과 기술단원에 STS에 관련된 내용이 가장 많았다. Piel에 따른 교과서별 STS 주제영역을 비교해 본 결과, 7개의 주제영역중에서 환경문제와 천연자원의 이용이 38.3%, 과학이 과학의 사회학이 29.6%, 기술발달의 영향이 23.6%, 에너지가 7.9%, 인간공학이 0.6%를 차지하고 있으며 인구 그리고 우주개발과 국방에 관련된 주제는 모든 교과서에서 다루고 있지 않았다. STS 주제를 단원별로 분석한 결과를 보면 에너지는 에너지 단원, 환경문제와 천연자원의 이용은 환경단원에 그리고 기술발달의 영향은 현대과학과 가술단원에 대부분 포함되어 있었다. 반면, 과학의 사회학은 전 단원에 걸쳐 골고루 포함되어 羚駭? STS 교육내용을 SATIS의 활동영역에 따라 분석해 보면 조사연구와 사례연구가 대부분이었고 현장활동, 역할놀이, 문제해결과 의사결정은 매우 적은 것으로 나타났다. The STS contents, emphasized in the 6th curriculum, in the chemistry parts of general science textbooks were analyzed. The STS contents of textbooks showed average of 24.4%. The chapter in "modern science and technology" were included 45.5% in STS contents, 38.7% in "environment", 29.1% in energy, and 14.0% in "materials". When the STS contents were analyzed by STS topics of Piel, the results are as follows; 38.3% on environmental quality and utilization of natural source, 29.6% on effect of technological developments, 7.9% on energy, and 0.6% on human engineering. However, there were no topics on population, space research and national defense. When the STS contents were analyzed by student activities of SATIS, most of the activities were research and case study. There were few field activities of role play, problem solving and decision making, and research design.

온라인 커뮤니티에 따른 영.유아 예방접종에 대한 정보습득 경로 및 지식수준 비교

최인영,정미은,최순,김석일,Choi, In-Young,Chung, Mi-Eun,Choy, Soon,Kim, Suk-Il 대한예방의학회 2007 예방의학회지 Vol.40 No.4

Objectives : To explore the information sources and knowledge on infant vaccinations of pro-vaccination community members and anti-vaccination community members on the internet. Methods : An online survey of 245 parents from three pro-vaccination communities and 92 parents from one anti-vaccination community was conducted from June 7 to June 23, 2006. Results : Parents from pro-vaccination communities usually gained the information regarding vaccination efficacy and risk mainly from healthcare providers (49.8%) and mass media (47.7%). Pro-vaccination community members considered healthcare providers as the most credible sources of information on vaccination, whereas the anti-vaccination community members usually gained their information regarding vaccine efficiency and risk from Internet child-care cafes and online vaccination communities. Parents of the anti-vaccination community considered the internet as the most credible information source (77.6% for efficacy, 94.8% for risk). In addition, the major reason why anti-vaccination community members didn't vaccinate and, will not vaccinate, was concern about possible side effects of the vaccine. The knowledge level on infant vaccination, education and economic status was higher in the anti-vaccination community. Conclusions : On-line communities concerned with vaccination are getting popular. The influence of anti-vaccination parents on the Internet is expected to be high. The government and healthcare providers need to increase their efforts to improve the credibility of information about vaccination. Our findings suggest that online communication regarding vaccinations needs to be considered as a means to increase vaccination rates.

고혈압 환자에서 혈압 조절 여부에 따른 신상(?象) 및 삶의 질 비교

최인영,한창호,최동준,정승현,신길조,이원철,Choi, In-Young,Han, Chang-Ho,Choi, Dong-Jun,Jung, Seung-Hyun,Shin, Gil-Jo,Lee, Won-Chul 대한한방내과학회 2009 大韓韓方內科學會誌 Vol.30 No.4

Objectives : This study is about a comparison of controlled and uncontrolled hypertension groups regarding pulse shape and quality of life. We aimed to find out if pulse shape would be able to help with hypertension treatment and care. Methods : We surveyed "controlled and uncontrolled hypertension patients" using SF-36, HTN QoL (measurement scale for the quality of life in hypertensive patients) and 3-D MAC pulse analyzer for pulse shape. Results : The uncontrolled hypertension group tended to have a sink pulse in the left cun. right guan & chi parts compared to the controlled hypertension group. Within the controlled hypertension group, patients who had a float pulse shape in both guan parts had a higher quality of life than patients who had a normal pulse shape. And the patients who had a normal pulse shape in both chi parts had a higher quality of life than patients with a slow or fast pulse shape. Within the uncontrolled hypertension grouP. the patients who had a normal pulse shape in right guan part had a higher quality of life than those with a choppy pulse shape. Nevertheless, these results were limited to only a few quality of life issues and were inconsistent in relation to specific pulse shapes. Conclusion : If we have more samples and better methods for collecting data, we will have better results, allowing us to improve our ability to predict and treat hypertension.

원거리 수소 가스 원격 계측을 위한 On-axis 라만 라이다 장치 개발

최인영,백성훈,임재영,차정호,김진호,Choi, In Young,Baik, Sung Hoon,Lim, Jae Young,Cha, Jung Ho,Kim, Jin Ho 한국광학회 2018 한국광학회지 Vol.29 No.3

수소 가스는 신 재생 에너지원으로서, 에너지의 발생과정에서 오염물질의 배출이 없는 친환경적인 에너지원이다. 그러나 수소 가스는 인화 에너지가 낮으며, 무색, 무취의 화염 전파성과 폭발성이 강한 매우 위험한 물질 중 하나이다. 수소 가스의 검출을 위한 다양한 기술이 있으나, 대부분 센서를 이용하여 대기 중의 수소 가스를 수집하여 측정하는 근거리 측정 기술이 대부분이다. 수소 가스 측정 기술 중 하나인 라만 라이다 장치는 수소 가스의 강한 라만 산란 현상을 이용하여 원거리에서 수소 가스 농도 검출 및 분포를 계측할 수 있는 장점을 갖고 있다. 본 연구에서는 넓은 영역에서(2~50 m) 누출된 수소 가스의 원거리 측정을 위한 on-axis 형태를 갖는 라만 라이다 장치를 개발하였다. 본 연구를 통하여 개발된 수소 가스 원거리 탐지 거리가 향상된 라만 라이다 장치의 성능을 검증하기 위하여, 수소 가스 폭발을 방지 및 농도 변화가 가능한 가스 챔버를 이용하여 라만 라이다 장치로부터 50 m 거리에 위치한 수소 가스 농도 측정 실험을 수행하였다. 그 결과, 개발된 라만 라이다 장치를 이용하여 50 m 거리에 위치한 0.66 Vol.%의 수소 가스 검출이 가능함을 증명하였다. Hydrogen gas is an important and promising energy resource that has no emissions of pollutants during power generation. However, hydrogen gas is very dangerous because it is colorless, odorless, highly flammable, and explosive at low concentration. Conventional techniques for hydrogen gas detection are very difficult for measuring the hydrogen gas distribution at long distances, because they sample the gas to measure its concentration. Raman lidar is one of the techniques for remotely detecting hydrogen gas and measuring the range of the hydrogen gas distribution. A Raman lidar system with an on-axis optical receiver was developed to improve the range of hydrogen gas detection at long distance. To verify the accuracy and improvement in the range of detecting the hydrogen gas, experiments measuring the hydrogen gas concentration are carried out using the developed on-axis Raman lidar system and a gas chamber, to prevent explosion of the hydrogen gas. As a result, our developed on-axis Raman lidar system can measure a minimum hydrogen gas concentration of 0.66 volume percent at a distance of 50 m.

운동실조와 현훈을 주소로 한 소뇌경색 환자의 척담탕 치험 1례

최인영,김미경,김보람,최동준,한창호,정승현,신길조,이원철,Choi, In-Young,Kim, Mi-Kyung,Kim, Bo-Ram,Choi, Dong-Jun,Han, Chang-Ho,Jung, Seung-Hyun,Shin, Gil-Jo,Lee, Won-Chul 대한중풍순환신경학회 2008 대한중풍.순환신경학회지 Vol.9 No.1

Ataxia and vertigo are frequent clinical findings in cerebellar infarction patients. In severe cases, they cannot achieve normal activity in daily life. A 65-year-old man was admitted with ataxia and vertigo diagnosed as acute cerebellar infarction. We prescribed for Chukdam-tang to treat the phlegm disease. After this treatment, ataxia and vertigo improved. We suggest Chukdam-tang is significantly effective on the treatment of cerebellar infarction.