Acid Phosphatase반응을 중심으로한 호중성 백혈구과립의 존재양상에 관한 전자현미경적 세포화학적 연구

최세영,손태중 慶北大學校 醫科大學 1972 慶北醫大雜誌 Vol.13 No.1

Acid Phosphatase반응을 중심으로한 호중성 백혈구과립의 존재양상에 관한 전자현미경적 세포화학적 연구

최세영,손태중 慶北大學校 醫科大學 1972 慶北醫大誌 Vol.13 No.1

It is evident that nentrophils participate in an important role in body defense mechanisms. The neutrophilic granules -lysosone- are thought as main machinery in the process of digestion of phagocytized foreign materials. The polymorp-honuclear leukocyte granules were investigated by examining developing granulocyte from normal rabbit bone marrow, in order to study how they exist, from where they are derived and how they develop. In addition, acid phosphosphatase activity was scored at electron microscpic level. The results were: Two distinct types of granules, azurophil and specific, were distinguished on the basis of their differences in size, density and time of origin. Both ypes originated from Golgi complex, but azurophil granules originated in the progranuloyte stage and specific granules originated in the myelocyte stage. No transforming features were found between the two types but azurophil granules decreased in number throughout successive maturation stages. Azurophil granules were more dense and larger than specific granules. Acid phosphatase activity was found in both immature azurophil and specific granules, as well as cisternae of rEr and Golgi complex, but mature azurophil and specific granules did not exhibit this activity.

관상동맥협착증에 있어서 관상동맥우회술의 치료효과

유영선,박진상,최세영,박창권,이광숙 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.4

Sixty-one patients with coronary artery stenosis underwent coronary artery bypass grafting from February 1986 to September 1993 at the department of Thoracic and Cardiovascular Surgery. There were 41 men and 20 women with a mean age of 54 years(range, 25 to 79 years). History of myocardial infarction was noted in 17 patients(28%). Twenty-eight patients(46%) had single-vessel disease, 24 patients(39%) had double-vessel disease and 9 patients(15%) had triple-vessel disease. The left main disease was in 4 patients(7%). Single aorta-coronary grafts were placed in 37 patients(60%), double or more grafts in 24 patients(40%). An average of 1.4 grafts were performed per patient. Saphenous vein grafts were employed in 49 patients(80%), and the internal mammary grafts, which were anastomosed to left anterior descending artery in 23 patients(38%). The mean bypass time was 135 minutes and cross-clamp time was 45 minutes. Early mortality was 16.4% (10 patients). Perioperative myocardial infarction was the most common complication, followed by atrial arrhythmia, wound infection, bleeding, pneumonia, pneumothorax. The 51 survivors were followed-up for 1 to 72 months, averaging 31 months. Ninety-five percent of the survivors were in New York Heart Association class I or Ⅱ postoperatively. There was one late death. Poor ejection fraction was significantly associated with hospital death(p<0.05).

종격동종양-28례 보고-

최세영,유영선,이광숙 啓明大學校 醫科大學 1984 계명의대학술지 Vol.3 No.1

For the purpose of evaluation of clinical characteristics and histopathological properties in mediastinal tumors, 28 patients with mediastinal tumors treated during the period from January 1979 to June 1984 were reviewed. There were 15 males and 13 females, and their ages ranged from 1 year to 53 years, with average age of 31 years. The most frequent tumors were neurogenic tumors, followed by thymic tumors, teratodermoids, benign cysts, and miscellaneous tumors in order of decreasing frequency. The benign tumors were 16 cases(57.1%) and malignant tumors were 12 cases(42.8%). 32.1% of the patients were asymptomatic at admission. There was no operative death.

사염화탄소 장기투여로 초래된 간 세포의 핵변화에 관한 전자 현미경적 연구

김종원,최세영,염우권,김필곤,민병훈 慶北大學校 醫科大學 1972 慶北醫大雜誌 Vol.13 No.1

사염화탄소 장기투여로 초래된 간 세포의 핵변화에 관한 전자 현미경적 연구

김종원,최세영,염우권,김필곤,민병훈 慶北大學校 醫科大學 1972 慶北醫大誌 Vol.13 No.1

The author has carried out light and electron microscopic investigation to delineate the intriguing nuclear modifications of hepatocyte after long-term exposure to carbon tetrachloride. The female Sprague-Dawley albino rats were received subcutaneous injections, twice a week for thirteen weeks, of 0.13㎖. 50 per cent solution of carbon tetrachloride in olive oil per 100㎎ of body weight. In each instance, the rats were sacrificed three days after the last injection. The control rats were not received any treatment. The results obtained are as follow: 1. The author's findings on fine structure of nucleus in hepatocyte of normal rats are almost similar to that of other investigators. 2. The morphological effects of long term administration of carbon tetrachloride upon the nucleus of hepatocyte are summerized as below. a)Two types of morphologically altered hepatocytes are encountered. One of these, corresponding to slightly basophilic cell in the H-E preparation, is characterized by proliferation of rough-surfaced endoplasmic reticulum. And the other is characterized by irregularly arranged, dilated and reduced in amount of rough-surfaced endoplasmic reticulum which is corresponded to esinophilic and lipid containing cell in the H-E preparation. However, the nuclear pattern observed in the two types of these cells are, in some respect, similar. b) The nucleus are usually enlarged and chromatin material is more clumped then in the controls tending to be marginated near the perinuclear membrane. c) The nuclear pores show prominant and numerous then are evident in control one. d) The nucleoli of the slightly basophilic cell are relatively large and occasionally multiple. e) The space delimited by the perinuclear membrane is often widened in lipid containing cells. and such nuclei frequently contain inclusions. The inclusions display multiple vesicle, lipid bodies or mitochondria-like figures which are delimited from the nuclear substance by a distinct single or double-layered membranes. f) These inclusions are thought to be as investigation of cytoplasmic components. 3. the nuclear modifications following longterm carbon tetrachloride administration are believed due to secondary to cytoplasmic derangement, which suggest active participation of nuclei in cytoplasmic modification found after prolonged exposure to carbon tetrachloride.

만성 통증관리를 위한 정맥내 Lidocaine 점적주사시 Lidocaine 혈중 농도, 제통효과 및 부작용 관찰

송선옥,전동석,최세영 대한마취과학회 1999 Korean Journal of Anesthesiology Vol.37 No.3

Background : Lidocaine administered intravenously (IV lidocaine) is efficacious in the treatment of thic pain. There are many differences in doses and methods of IV lidocaine therapy. We have made a ous clinical report of IV lidocaine infusion for chronic pain patients. The aims of this study were to evaluate the plasma concentration of lidocaine, and the analgesic effect and safety of our method of IV idocaine infusion. Methods : Sixteen neuropathic pain patients received IV lidocaine infusion. Lidocaine of 5 mg/kg ixed in 150 ml of normal saline was infused over 40 min at a rate with 300 ml/h for the initial 10 min, and the remaining at 200 ml/h. Blood sampling, for the analysis of plasma lidocaine concentration, pain score by numerical rating scale, blood pressure and heart rate were obtained before the infusion and at 20, 40, 60, 90 and 120 min following the start of infusion. Results : Thirteen patients (81.3%) had analgesic effects in IV lidocaine infusion. Mean plasma caine concentrations were 0, 2.0, 2.7, 2.2, 1.5, 1.1 ug/ml, and mean pain scores were 7.6, 5.6, 3.7, 3.1, 3.0, and 3.1 before the infusion and at 20, 40, 60, 90 and 120 min following the start of infusion. Plasma aine over 2 μg/ml revealed an analgesic effect, and pain scores precipitously dropped around 40 min following the start of infusion. There were no significant changes of blood pressure and heart rate. Side effects were mild in terms of sedation, dizziness, light-headedness, nausea and metallic taste. Conclusions: These results suggest that our method of IV lidocaine infusion -within therapeutic nge of lidocaine not to allow toxic plasma concentration, and with any effective analgesia, little modynamic change and minimal side effects- is a useful and a safe diagnostic and therapeutic modality for hronic neuropathic pain. (Korean J Anesthesiol 1999; 37: 459∼466)