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<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>
Good health and longevity are the goal of human beings. Dietary treatment for the improvement of health and chronic diseases therapy has been occupied an important position in Oriental and Western medicine. In this paper, the viewpoints of dietary treatment in Oriental and Western medicine were compared. The principle of Yin-Yang and five elements, as a standing rule that establishes the theory of Oriental medicine and origin of the oriental philosophy, it provides the based of the Oriental medicine's outlook of the world. The principles of dietary treatment in Oriental medicine was reasonable combination of food, balance of Oh-Mi and temperance of food. Nutritional therapy in Oriental medicine has done the phase of Bian Zheng by individual characteristics. The theory of Yin-Yang and five elements, Ki-Mi, Sasang Constitution Medicine and Kyi-Kyung were obviously recognized the theory of nutrition therapeutics in Oriental medicine. In Western medicine, dietary treatment supplies individuals with a recipe of calorie and specific nutrients. The Food Exchange System was very convenient way of practice in nutrition therapy. But each of dietary treatment in Oriental and Western nutrition has strong and week points. So we need to study enough materials to treatment of Oriental nutrition and Western nutrition for the improvement of health and chronic diseases therapy.
이경성과 크리에이티브 바키(Creative VaQi)의 연극은 희곡의 재현이 아니라 `공간`에 대한 질문에서 출발한다. 광화문이나 문화역 서울과 같은 일상적 공간 속에서 행해졌던 그의 작업은 이들 공간으로부터 고유의 장소성, 즉 그 공간에 축적된 역사적, 사회적 맥락을 드러냈다. 이후 그들은 일상적 공간으로부터 극장이라는 제도적 공간으로 중심을 옮겨 극장의 장소성에 대해 근본적인 질문을 던지는데, 이것은 연극이 무엇인가에 대한 자기질문이기도 하다. 이로써 이경성과 바키의 연극은 예의 그 `장소특정적 공연`의 카테고리를 벗어나 보다 더 미학적 지평을 확장해가고 있다. 그 과정에서 우리는 그들의 작업을 공간과 함께, 좀더 시각을 달리하여 공간을 점유한 몸의 문제를 근거로 새롭게 논의할 수 있는 단초를 얻게 된다. 사실 공간에 대한 질문에서 출발한 바키의 작업은 애초부터 그 공간을 점유하고 있는 배우의 몸을 배제하고는 논의할 수가 없는 것이었다. 본 논문은 크리에이티브 바키의 연극을 배우, 배우의 몸으로부터 새롭게 논의하고자 한다. 통상적 연극과 다르게 그들은 연출과 배우 모두가 직접 공연 전 과정을 이끌면서 관련 자료를 조사하고 인터뷰하며, 여러 번의 발제와 토론 등의 과정을 거쳐 공연의 틀을 잡아간다. 이런 작업과정에서 배우는 어떻게 대상을 체험하는지, 그리고 체화된 감각을 바탕으로 무대를 그들 연극의 주요 화두인 공간과 시간, 더 나아가 타자에 대한 감각의 장소로 발생시키는지를 살펴보고자 한다. 무대 위의 배우의 몸은 공간을 점유한 몸으로서 `여기`에 대해 질문하기도 하고, 시간을 점유하고 `지금`에 대해 묻는다. 그런가하면 보이지 않는 타자의 고통을 감각하는 몸으로서, 기억에 대해 묻기도 한다. 배우의 몸에 기반한 이경성의 연출미학을 풀어내는 이론적 근거는 몸과 지각의 문제를 중심에 둔 현상학에서 가져왔으며, 구체적인 사례들은 최근 2년 동안 발표된 이들의 작품들에서 찾아보았다. Lee Kyung-Sung and the theatergroup `Creative VaQi) have made their theaters focusing on space discourses. But for about last two years, most of their works have been performed not only in everyday spaces but also in various instituional theaterspaces. This is why the research about their theater aesthetics should be conducted from different perspectives. This study examines several recent works that Lee Kyung Sung and his VaQi have performed in institutional theatrical spaces. Actor`s bodies that occupy the space have been their theatrical interest that they have focused on from the beginning. What they emphasize is not how they make actors represent their drama roles. They see actors not as tools of theater but as productive subjects. Actors in their theaters stand on stage as themselves with their own identities. In other words, they reveal themselves on stages as persons who have their own thoughts about the world. This is due to their unique working style that are completed through thorough collaborative work, from data research to interviews, presentations and discussions. Of course, actors are the center of all these processes. They constantly interact with objects and build their own viewpoint on them. The body itself is a performing body that creates events and forms the framework of perception and experience. This way of working is closely linked to the phenomenological point of view. Based on this phenomenological point of view, this paper examines how actor`s body is posing new questions about space and time. It tries also to look at how actor`s body occurs as a place of sensation for the other.
This study examines the costume artifacts discovered in the burial site of Choi Kyung Sun(1561~1622), which was moved from Gangwon in Gangrung Province. About 30 artifacts, including costumes, were recovered from Choi Kyung Sun's grave. The collection of men's coats discovered in the grave consisted of a Dallyeong (Official's robe), Jing-nyeong(Man's coat), Jungchimak(Man's coat), Cheolrik(Man's coat), Chang-ui(Man's coat), Gwang-su-ju-ui(Man's coat), and Do-po(Man's coat). Han-sam(Undershirts), Jeo-go-ri(Jacket), and trousers were also recovered, along with several items made of textiles, such as the cloth used to shroud and wrap the body. The costume artifacts recovered from Choi Kyung Sun's grave have several characteristics that distinguish them from other early 17th-century costume artifacts. Firstly, the body had a hairband made from horsehair. Secondly, the men's trousers were of the type worn before the Japanese Invasion(1592~1597). Thirdly, the style of the coats' sleeves, when compared with artifacts from other regions, was from an earlier era. Through these costume artifacts, it can be inferred that people who lived in the Gangwon Province, which was far away from Seoul, continuously wore outfits from before the Japanese Invasion, and trends spread slowly.
<P><B>Abstract</B></P><P>Calmodulin-regulated protein phosphorylation plays a pivotal role in amplifying and diversifying the action of calcium ion. In this study, we identified a calmodulin-binding receptor-like protein kinase (CBRLK1) that was classified into an S-locus RLK family. The plasma membrane localization was determined by the localization of CBRLK1 tagged with a green fluorescence protein. Calmodulin bound specifically to a Ca<SUP>2+</SUP>-dependent calmodulin binding domain in the C-terminus of CBRLK1. The bacterially expressed CBRLK1 kinase domain could autophosphorylate and phosphorylates general kinase substrates, such as myelin basic proteins. The autophosphorylation sites of CBRLK1 were identified by mass spectrometric analysis of phosphopeptides.</P><P><B>Structured summary</B></P><P>MINT-6800947:<I>CBRLK1</I> (uniprotkb:Q9ZT06) and <I>AtCaM2</I> (uniprotkb:P25069) <I>bind</I> (MI:0407) by <I>electrophoretic mobility shift assay</I> (MI:0413)</P><P>MINT-6800966:<I>AtCaM2</I> (uniprotkb:P25069) and <I>CBRLK1</I> (uniprotkb:Q9ZT06) <I>bind</I> (MI:0407) by <I>competition binding</I> (MI:0405)</P><P>MINT-6800930:<I>CBRLK1</I> (uniprotkb:Q9ZT06) <I>binds</I> (MI:0407) to <I>AtCaM2</I> (uniprotkb:P25069) by <I>far Western blotting</I> (MI:0047)</P><P>MINT-6800978:<I>AtCaM2</I> (uniprotkb:P25069) <I>physically interacts</I> (MI:0218) with <I>CBRLK1</I> (uniprotkb:Q9ZT06) by <I>cytoplasmic complementation assay</I> (MI:0228)</P>
Kim,,Min,Jeong,Kwon,,Mi,Jung,Kang,,Ho,Suk,Choi,,Kyung,Chan,Nam,,Eun,Sook,Cho,,Seong,Jin,Park,,Hye-Rim,Min,,Soo,Kee,Seo,,Jinwon,Choe,,Ji-Young,Park,,Hyoung-Chul Hindawi 2018 BioMed research international Vol.2018 No.-
<P>Mitotic counts in the World Health Organization (WHO) grading system have narrow cutoff values. True mitotic figures, however, are not always distinguishable from apoptotic bodies and darkly stained nuclei, complicating the ability of the WHO grading system to diagnose well-differentiated neuroendocrine tumors (NETs). The mitosis-specific marker phosphohistone H3 (PHH3) can identify true mitoses and grade tumors reliably. The aim of this study was to investigate the correspondence of tumor grades, as determined by PHH3 mitotic index (MI) and mitotic counts according to WHO criteria, and to determine the clinically relevant cutoffs of PHH3 MI in rectal and nonrectal gastrointestinal NETs. Mitotic counts correlated with both the Ki-67 labeling index and PHH3 MI, but the correlation with PHH3 MI was slightly higher. The PHH3 MI cutoff ≥4 correlated most closely with original WHO grades for both rectal NETs. A PHH3 MI cutoff ≥4, which could distinguish between G1 and G2 tumors, was associated with disease-free survival in patients with rectal NETs, whereas that cutoff value showed marginal significance for overall survival in patient with rectal NETs. In conclusion, the use of PHH3 ≥4 correlated most closely with original WHO grades.</P>
안혜연 ( Hye Yeon An ), 정주희 ( Ju Hee Jeong ), 김태희 ( Taehee Kim ), 윤진아 ( Jinah Yun ), 김현수 ( Hyunsu Kim ), 오인보 ( Inbo Oh ), 이지호 ( Jiho Lee ), 원경미 ( Kyung Mi Won ), 이영미 ( Young Mi Lee ), 김유근 ( Yoo Keun Kim ) 한국환경과학회 2016 한국환경과학회지 Vol.25 No.6
The effect of weather on disease was investigated based on results reported in academic papers. Weather-sensitive disease was selected by analyzing the frequency distributions of diseases and correlations between diseases and meteorological factors (e.g., temperature, humidity, pressure, and wind speed). Correlations between disease and meteorological factors were most frequently reported for myocardial infarction (MI) (28%) followed by chronic ischemic heart disease (CHR) (12%), stroke (STR) (10%), and angina pectoris (ANG) (5%). These four diseases had significant correlations with temperature (meaningful correlation for MI and negative correlations for CHR, STR, and ANG). Selecting MI, as a representative weather-sensitive disease, and summarizing the quantitative correlations with meteorological factors revealed that, daily hospital admissions for MI increased approximately 1.7%-2.2% with each 1℃ decrease in physiologically equivalent temperature. On the days when MI occurred in three or more patients larger daily temperature ranges (2.3℃ increase) were reported compared with the days when MI occurred in fewer than three patients. In addition, variations in pressure (10 mbar, 1016 mbar standard) and relative humidity (10%) contributed to an 11%-12% increase in deaths from MI and an approximately 10% increase in the incidence of MI, respectively.
Obesity may be the consequence of various environmental or genetic factors, which may be highly correlated with each other. We aimed to examine whether grandmaternal and maternal obesity and environmental risk factors are related to obesity in daughters. Daughters (n = 182) recruited from female students, their mothers (n = 147) and their grandmothers (n = 67) were included in this study. Multivariable logistic regression was used to analyze the association between the daughter's obesity and maternal, grandmaternal, and environmental factors. Maternal heights of 161-175cm (OD: 8.48, 95% CI: 3.61-19.93) and 156-160 cm (2.37, 1.14-4.91) showed positive associations with a higher height of daughter, compared to those of 149-155 cm. Mothers receiving a university or a higher education had a significant OR (3.82, 1.27-11.50) for a higher height of daughter compared to those having a low education (elementary school). Mother having the heaviest weight at current time (59-80 kg, 3.78, 1.73-8.28) and the heaviest weight at 20 years of age (51-65 kg, 3.17, 1.53-6.55) had significant associations with a higher height of daughters, compared to those having the lightest weight at the same times. There was no association between the height, weight, and BMI of daughters and the characteristics and education of her grandmothers. In conclusion, although genetic factors appear to influence the daughter's height more than environmental factors, the daughter's weight appears to be more strongly associated with individual factors than the genetic factors.
( Mi Ae Kim ), ( Ji Hyun Lee ), ( Hye Cheol Jeong ), ( Seung Won Koo ), ( Kyung Mi Park ), ( Sang Ho Cho ), ( Hyeon Jae Lee ), ( Eun Kyung Kim ) 대한결핵 및 호흡기학회 2011 Tuberculosis and Respiratory Diseases Vol.70 No.6
The fat-forming variant of solitary fibrous tumors (SFTs) is a rare soft tissue neoplasm that was previously referred to as a lipomatous hemangiopericytoma (L-HPC). The most common affected site is deep soft tissue. Here, we present the first case, worldwide, of a fat-forming variant of SFT of the pleura. A 74-year-old man presented with left lower chest pain. Chest radiographs showed a mass-like lesion at the left lower lung field and chest computed tomography revealed a 12 cm fat-containing enhancing mass that was well-separated, lobulated and inhomogeneous. Radiology findings suggested a liposarcoma. Percutaneous needle biopsy was performed and pathological diagnosis of the mass was a fat-forming variant of SFT. Surgical resection was carried out and there has been no recurrence to date. So, a benign fat-forming variant of SFT must be considered as one of the differential diagnoses of lipomatous tumors of the pleura.